Q254I9 (LPXD_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	CF0527

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264358
Sites
	Active site	248	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q254I9 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: BE6AC7E69F0C3ECC	FASTA	359	38,886
	10 20 30 40 50 60 MPQELVYTLQ QLADLLKVEV QGNTETPISG VEEISEAQSH HVTFLDNEKY SRFIKNTEAG 70 80 90 100 110 120 AIILSKAQAQ KYGHLNKNFL IVSEFPSIAF QKCIELFIPP IDSGFPGIHP TAVIHPTAHI 130 140 150 160 170 180 GKDVFLEPYA VICQHAQIGD SSHIGAGSVI GAFSTLGEHC YVHPKVVIRE RVVIGKRVIV 190 200 210 220 230 240 QPGAIIGACG FGYITNAFGR HKHLKHLGQV IIEDDVEIGA NTTIDRGRFK NSVIREGTKI 250 260 270 280 290 300 DNQVQIAHHV EVGKHSMIVA QAGIAGSTKI GNHVIIGGQT GITGHISITD HVIMMAQTGV 310 320 330 340 350 TKSISSPGIY GGAPARPYQE IHRQVAKIRG LPKLEERLGM LEEKVKGLSA QSEEAQITP

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fe/C-56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006861 Genomic DNA. Translation: BAE81299.1.
RefSeq	YP_515444.1. NC_007899.1.
3D structure databases
ProteinModelPortal	Q254I9.
SMR	Q254I9. Positions 7-348.
ModBase	Search...
Protein-protein interaction databases
STRING	Q254I9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3958328.
GenomeReviews	Gene locus CF0527 in contig AP006861_GR.
KEGG	cfe:CF0527.
PATRIC	20204693. VBIChlFel51660_0553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q254I9.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	CFEL264202:CF0527-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_CHLFF

Accession

Primary (citable) accession number: Q254I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program