Reviewed, UniProtKB/Swiss-Prot Q254B9 (AMPA_CHLFF)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	CF0597

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019904
Sites
	Active site	276	1	Potential
	Active site	350	1	Potential
	Metal binding	264	1	Manganese 2 By similarity
	Metal binding	269	1	Manganese 1 By similarity
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	287	1	Manganese 2 By similarity
	Metal binding	346	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q254B9-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 7CD627209DC1BD65	FASTA	500	54,235
	10 20 30 40 50 60 MVLFHSQASC CKRVKADAIV LPFWQVQDKV RCAASIVEEY EPLYQVALEN FAGKTGEAEL 70 80 90 100 110 120 IYSYGQGKEK RILLLGLGKN EELSSQDVLE AYAKATRILR KAKCTTVNIV LPVISELRIS 130 140 150 160 170 180 AEDFLSNLTS GILSLNYNYP KYTKECQSSE PLLTKVTVLG IVPKIADRIF RKEESIFEGV 190 200 210 220 230 240 YLTRDLVNGN ADEVTPQKLA NIAKGLAKEF PSVDAKILNK DAILKEKMGL LAAVAKGSAV 250 260 270 280 290 300 DPCFIVLSYQ GKPKSKDHTV LIGKGVTFDS GGLDLKPGKA MLTMKEDMAG AATALGILSG 310 320 330 340 350 360 VAALELPVNV TAIIPATENA IDGASYKMGD VYIGMSGLSV EIGSTDAEGR LILADAITYA 370 380 390 400 410 420 LKYCKPTRII DFATLTGAMV VSLGESVAGF FSNNDVLAQD LSEASAETGE ALWRLPLVEK 430 440 450 460 470 480 YDKALQSDIA DMKNIGSNRA GAITAALFLR RFLEDQPVAW AHLDIAGTAY REKDEDPYPK 490 500 YASGFGVRCL IYYIEKFLSK

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006861 Genomic DNA. Translation: BAE81369.1.
RefSeq	YP_515514.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q254B9.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	3959155.
GenomeReviews	Gene locus CF0597 in contig AP006861_GR.
KEGG	cfe:CF0597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q254B9.
OMA	ECEDLGM.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CHLFF

Accession

Primary (citable) accession number: Q254B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)