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Q25490 (APLP_MANSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apolipophorins

Cleaved into the following 2 chains:

  1. Apolipophorin-2
    Alternative name(s):
    Apolipophorin II
    apoLp-2
  2. Apolipophorin-1
    Alternative name(s):
    Apolipophorin I
    apoLp-1
OrganismManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifier7130 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Sphingidae › Sphinginae › Sphingini › Manduca

Protein attributes

Sequence length3305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes the major component of lipophorin, which mediates transport for various types of lipids in hemolymph. Acts by forming lipoprotein particles that bind lipoproteins and lipids. May be required for morphogens wingless (wg) and hedgehog (hh) function, possibly by acting as vehicles for the movement of wg and hh By similarity.

Subcellular location

Secreted.

Post-translational modification

Cleaved into 2 chains by furin protease. However, prevention of cleavage does not impair its function By similarity.

N-glycosylated By similarity.

Sequence similarities

Contains 1 vitellogenin domain.

Contains 1 VWFD domain.

Ontologies

Keywords
   Biological processLipid transport
Transport
Wnt signaling pathway
   Cellular componentSecreted
   DomainSignal
   LigandLipid-binding
   PTMCleavage on pair of basic residues
Glycoprotein
Gene Ontology (GO)
   Biological_processWnt receptor signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlipid binding

Inferred from electronic annotation. Source: UniProtKB-KW

lipid transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 711688Apolipophorin-2 By similarity
PRO_0000041530
Chain712 – 33052594Apolipophorin-1 By similarity
PRO_0000041531

Regions

Domain39 – 640602Vitellogenin
Domain2734 – 2929196VWFD

Sites

Site711 – 7122Cleavage; by furin By similarity

Amino acid modifications

Glycosylation6431N-linked (GlcNAc...) Potential
Glycosylation27691N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q25490 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B27D611410285FD7

FASTA3,305366,947
        10         20         30         40         50         60 
MGKSNRLLSV LFVISVLWKA AYGNGKCQIA CKGSSSPSFA AGQKYNYGVE GTVSVYLTGA 

        70         80         90        100        110        120 
DNQETSLKML GQASVSAISN CELELSVHNM VLSGPDGKKY PCPQGIEKPV RFSYQDGRVG 

       130        140        150        160        170        180 
PEICAAEDDS RRSLNIKRAI ISLLQAEQKP SVQVDVFGVC PTEVSSSQEG GAVLLHRSRD 

       190        200        210        220        230        240 
LSRCAHREQG RNDFVNSIAN PDAGIKDLQV LQSMLNVESK VNNGVPEKVS AIEEYLYKPF 

       250        260        270        280        290        300 
SVGENGARAK VHTKLTLSGK GGAGGGNAHC TESRSIIFDV PHGTSSASGN LNSVISAVKE 

       310        320        330        340        350        360 
TARTVANDAS SKSAGQFAQL VRIMRTSSKD DLMRIYSQVK AHQLEKRVYL DALLRAGTGE 

       370        380        390        400        410        420 
SIEASIQILK SKDLSQLEQH LVFLSLGNAR HVNNPALKAA AGLLDMPNLP KEVYLGAGAL 

       430        440        450        460        470        480 
GGAYCREHDC HNVKPEGIVA LSNKLGSKLQ NCRPKNKPDE DVVVAILKGI RNIRHLEDSL 

       490        500        510        520        530        540 
IDKLVHCAVD NNVKARVRAV ALEAFHADPC SAKIHKTAMD IMKNRQLDSE IRIKAYLAVI 

       550        560        570        580        590        600 
ECPCSHSASE IKNLLDSEPV HQVGNFITSS LRHIRSSSNP DKQLAKKHYG QIRTPNKFKV 

       610        620        630        640        650        660 
DERKYSFYRE MSYKLDALGA GGSVDQTVIY SQTSFLPRSV NFNLTVDLFG QSYNVMELGG 

       670        680        690        700        710        720 
RQGNLDRVVE HFLGPKSFLR TEDPQALYDN LVKRFQESKK KVEDSLSRGR RSIKSEIDVF 

       730        740        750        760        770        780 
DKNLKAESAP YNNELDLDIY VKLFGTDAVF LSFGDDKGFD FNKMLDQILG GCNSGINKAK 

       790        800        810        820        830        840 
HFQQEIRSHL LFMDAELAYP TSVGLPLRLN LIGAATARLD VATNIDIRQI FQSPQNAKAD 

       850        860        870        880        890        900 
IKFVPSTDFE ISGAFIIDAD AFSTGIKVIT NLHSSTGVHV NAKVLENGRG IDLQIGLPVD 

       910        920        930        940        950        960 
KQELIAASSD LVFVTAEKGQ KEKQKVIKME KGENEYSACF DQLSGPLGLT MCYDMVLPFP 

       970        980        990       1000       1010       1020 
IVNRNDKLDS IAKAMGKWPL SGSAKFKLFL EKNDLRGYHI KAVVKEDKDA GRRSFELLLD 

      1030       1040       1050       1060       1070       1080 
TEGAKTRRSQ LTGEAVYNEN EVGVKLGLEA VGKVIYGHIW AHKKPNELVA SVKGKLDDIE 

      1090       1100       1110       1120       1130       1140 
YSGKLGFSVQ GNEHRAVYKP IFEYSLPDGS SPGSKKYEVK IDGQVIRECD GRVTKYTFDG 

      1150       1160       1170       1180       1190       1200 
VHVNLQNAEK PLEICGSVST VAQPREVEFD VEVKHYASLK GSWKGSDVVL AFNNQLNPKI 

      1210       1220       1230       1240       1250       1260 
NFDLKGKFEN TDSMHNELDI HYGPNRGDNN ARITFSQILK YHVENSKNFN VITKNNLEIR 

      1270       1280       1290       1300       1310       1320 
AVPFKLVANA DVDPKKIDID IEGQLQDKSA GFNLDARTHI KKEGDYSIKV KANLNNANLE 

      1330       1340       1350       1360       1370       1380 
AFSRRDIVNA EKSNVENYID MKGVGRYELS GFVLHKTKPN DVNVGFIGHL KINGGGKNED 

      1390       1400       1410       1420       1430       1440 
FKINIGHIET PAVFSSHATI SGSRGDIIDY LLKIMRTANP NGNFKLVIKD SIAANGQYKV 

      1450       1460       1470       1480       1490       1500 
TDADGKGNGL IIIDFKKINR KIKGDVRFTA KEPVFNADID LFLNFEKDNS DKVHFSTYNK 

      1510       1520       1530       1540       1550       1560 
KTDKVMDTKN KLEYAGKRTE VNIHQDGILA VTGKAHTVAE LVLPTERCLS LKIDHDGAFK 

      1570       1580       1590       1600       1610       1620 
DGLYNGHMDM TISDAPKRGS GASTISYKGK VSNSNLDQEI IDYEGQINFK LKDGKNLQST 

      1630       1640       1650       1660       1670       1680 
FSLKNNPDGD KFKYEFKSDV NGNLIPKPAN LVATGTYSNS ENEIDETYRL KGSYGSDIGF 

      1690       1700       1710       1720       1730       1740 
ELAGVGTIKF LDAGDKKYLD DYTLTVRLPF EKAHDIKWVS TVLFLQPQGQ EMTEYTLVES 

      1750       1760       1770       1780       1790       1800 
VQINADVYKI DANGKVGPKN GYGAVKVLVP HVEPFVLDYN YKSSHEGEKN NNYVELKTKY 

      1810       1820       1830       1840       1850       1860 
GKGKSASMVV DSSYAPHYST LKVKANTPNN DKFKKLDVTV HSKNPSPDAY SNSVVVDADG 

      1870       1880       1890       1900       1910       1920 
RVYKIDSSIV LSKAHPVLDI QYHSPSSDKI RRLYLQGSSL SSTQGKLEVK VDNINDICLD 

      1930       1940       1950       1960       1970       1980 
AVSEANVQKD NVAFKVVANA KELGWKNYGI DISSKDSGSG KRLEFHATND NKNVLSGSTS 

      1990       2000       2010       2020       2030       2040 
FISKQEGQKT IIEGSGSVKV KEEQKSANFK YIRTVFTDSN EKGVETFFNV ALGERSYVAE 

      2050       2060       2070       2080       2090       2100 
SRVTNYEYKN SYVYCEEKKQ CAHAEIQSKI DMSTPGMIVN VINAGLDLRK LGVAPELGLQ 

      2110       2120       2130       2140       2150       2160 
MRDEVSDRRP PRFTLDLHIN KEDRKYHLHA YNTPENGHYA SGVTVRLPSR VMALEYTLTH 

      2170       2180       2190       2200       2210       2220 
PTSQDLPFPI KGEACLDLDK NRPGHKTSAR FLVDYSNSGS EDKAVAEIGF FHPKIEKEAV 

      2230       2240       2250       2260       2270       2280 
IRLNAFMKRP ENGCFKIESS ASLCHSALGT DRVAKVMFET TPNSVKFLAD TPFVKAIDVE 

      2290       2300       2310       2320       2330       2340 
GSFNVNQQQR TQQCLFRICL LEGKPVQMSA LVKDYQYYEF TTEESNRKLS YVGHLIPEKR 

      2350       2360       2370       2380       2390       2400 
VDISTDIILS GDKKNIAHGA LFLQDNLVKS DYGLSKENFN YFLNALKKDL DTLEDRIKNV 

      2410       2420       2430       2440       2450       2460 
GEKASKDVEA VTQRAAPYFK KVEDNFRAEW NRFYQEIADD KVFKEISHVF NEIVQYIAKF 

      2470       2480       2490       2500       2510       2520 
IDEILQGTKR SWTPSCRPTL SHPRNREMYK KQIEPQVKQL YDTLGALMKE YLDGVIDVVA 

      2530       2540       2550       2560       2570       2580 
HFAAIVTDFF EKHKAELQEL TNVFTEIFKD LTRLVVAQLK ELPPKIAQIY NDIVSQITNM 

      2590       2600       2610       2620       2630       2640 
PFVVVLQEKW KEFNFAERAV QLVSQAYEAF SKILPTDELK EFAKALNAYL LKKIKEEKME 

      2650       2660       2670       2680       2690       2700 
ESKELPRAVR EAGQRVLLIT SIPALAVRRP RLRRWTWHHL KLAVGAGASA PSLGAASWSA 

      2710       2720       2730       2740       2750       2760 
LRQLAAGDGP PALAPRGLPT AQLDPLDEVP NKLRAVVVNG QHIFTFDGRH LTFPGTCRYV 

      2770       2780       2790       2800       2810       2820 
LIHDHVDRNF TVLMQLANGQ PKALVLEDKS GTIIELKDNG QVILNCQSHG FPVVEQDVFA 

      2830       2840       2850       2860       2870       2880 
FRQTSGRIGL CSKYGLMAFC TSKFEVCYFE VNGFYLGKLP GLLGDGNNEP YDDFRMPNGK 

      2890       2900       2910       2920       2930       2940 
ICSSESEFGN SYRLSRSCPA ANAPAHDHHQ MHAPLPKPCE RVFSGTSPLR PLSLMLDIAP 

      2950       2960       2970       2980       2990       3000 
FRQACIHAVT GADADKDLQQ ACDLARGYRR SRSRGCCPPR CPTPACAART ATGPGSWATP 

      3010       3020       3030       3040       3050       3060 
TSTNCPTDSL ISSSPLRPLR TTPAHYKNMV VPLVSQLVDM LKGKHCTDIK VFLVGHTSKH 

      3070       3080       3090       3100       3110       3120 
PYPILYDTDL KLKNAKVSFD DKSRYDRIPF VKTGHEKFDS YSKTVVDFLN YIKIELGITN 

      3130       3140       3150       3160       3170       3180 
IEASQGQIFD LPLRPGAVKH VIFVTGGPTI SQFFLLETVR ALRNKVIIDE MAMSASLVTS 

      3190       3200       3210       3220       3230       3240 
TPGLKIGGGK NAAQIVGYEK HGVLLLGEKK QSKDSEAVRA TLEVEDDPFS DAVEFANGVV 

      3250       3260       3270       3280       3290       3300 
FSASNYAALP AGQQKQFIQT AAHNIIQRMW REQIVQQCTC VFVDPFRVRS VCFNKARTEV 


ARRRK

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References

[1]"The precursor protein of the structural apolipoproteins of lipophorin: cDNA and deduced amino acid sequence."
Sundermeyer K., Hendricks J.K., Prasad S.V., Wells M.A.
Insect Biochem. Mol. Biol. 26:735-738(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fat body.

Web resources

Protein Spotlight

Lipid freight - Issue 59 of June 2005

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U57651 mRNA. Translation: AAB53254.1.
PIRT18358.

3D structure databases

ProteinModelPortalQ25490.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.25.10.20. 1 hit.
2.20.50.20. 2 hits.
2.20.80.10. 1 hit.
2.30.230.10. 1 hit.
InterProIPR015819. Lipid_transp_b-sht_shell.
IPR001747. Lipid_transpt_N.
IPR009454. Lipid_transpt_open_b-sht.
IPR015816. Vitellinogen_b-sht_N.
IPR015255. Vitellinogen_open_b-sht.
IPR015817. Vitellinogen_open_b-sht_sub1.
IPR015818. Vitellinogen_open_b-sht_sub2.
IPR011030. Vitellinogen_superhlx.
IPR001846. VWF_type-D.
[Graphical view]
PfamPF06448. DUF1081. 1 hit.
PF09172. DUF1943. 1 hit.
PF01347. Vitellogenin_N. 1 hit.
PF00094. VWD. 1 hit.
[Graphical view]
SMARTSM00638. LPD_N. 1 hit.
SM00216. VWD. 1 hit.
[Graphical view]
SUPFAMSSF56968. Lipid_transp_b-sht_shell. 2 hits.
SSF48431. LV_superhelical. 1 hit.
PROSITEPS51211. VITELLOGENIN. 1 hit.
PS51233. VWFD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPLP_MANSE
AccessionPrimary (citable) accession number: Q25490
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents