Reviewed, UniProtKB/Swiss-Prot Q253Y4 (MURE_CHLFF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CF0632

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012344
Regions
	Nucleotide binding	111 – 117	7	ATP Potential
	Region	153 – 154	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	405 – 408	4	Meso-diaminopimelate binding By similarity
	Motif	405 – 408	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	32	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	180	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	188	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	382	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q253Y4-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 91A8312A531EFE64	FASTA	485	53,404
	10 20 30 40 50 60 MNLKELLHNI DIDAKVYGKI SPIEVRNLTK DSRNIGFGDI FIANKGKRCD GNDFASLAVE 70 80 90 100 110 120 NGAIAVVSSI YNPFLSVVQI ISPNLSLLEA QLAAKYYNYP SRKLCVVGIT GTNGKTTVSH 130 140 150 160 170 180 LIKFLFDACD KPSGLIGTIE HILGNNRIQD GFTTPESCLL QKYLAEMVKS NLTAAVIETS 190 200 210 220 230 240 SIGLVLDRLA NVEFDVGVLT NVTLDHLDFH DSFEEYIKAK LQLFAQLSDS GLAVVNNDLP 250 260 270 280 290 300 QAKQFLEATR AVPVTYGIEQ LADYRASNLR FSPFGADFDL IHKGEIFPCH SPLIGQYNVY 310 320 330 340 350 360 NVLAAIAVTH QRLNCDLQQL VSLVASVKSP RGRLEPIQSG PCPIYIDYAH TPDALDNVCQ 370 380 390 400 410 420 TLRTLLPSGG KLIVVFGCGG DRDRSKRKIM AQVVEKYGFA VVTSDNPRGE DPETIVNEIC 430 440 450 460 470 480 SGFVKRNFSI EIDRKQAITY ALSIASDRDI VLVAGKGHET YQIFKHQTIA FDDREVVHEV LSSYV

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006861 Genomic DNA. Translation: BAE81404.1.
RefSeq	YP_515549.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q253Y4.
Genome annotation databases
GeneID	3958925.
GenomeReviews	Gene locus CF0632 in contig AP006861_GR.
KEGG	cfe:CF0632.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q253Y4.
OMA	FPVIVDY.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_CHLFF

Accession

Primary (citable) accession number: Q253Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)