Reviewed, UniProtKB/Swiss-Prot Q25378 (KPC1_LYTPI)

Names and origin

Protein names

Recommended name:
    Protein kinase C
    EC=2.7.11.13

Gene names

Name:

PKC1

Organism

Lytechinus pictus (Painted sea urchin)

Taxonomic identifier

7653 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Echinoidea › Euechinoidea › Echinacea › Temnopleuroida › Toxopneustidae › Lytechinus

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme By similarity. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Protein kinase C		PRO_0000055729
Regions
	Domain	164 – 251	88	C2
	Domain	325 – 583	259	Protein kinase
	Domain	584 – 654	71	AGC-kinase C-terminal
	Zinc finger	27 – 77	51	Phorbol-ester/DAG-type 1
	Zinc finger	92 – 142	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	331 – 339	9	ATP By similarity
Sites
	Active site	449	1	Proton acceptor By similarity
	Binding site	354	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q25378-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74B5A27A49C835A2	FASTA	658	74,871
	10 20 30 40 50 60 MSNSTLEMKG FARRGALRQK NVHEIKNHKF IPRFFKQPTF CSHCKDFIWG FGKQGFQCKV 70 80 90 100 110 120 CSFVVHKRCH EFVTFQCPGL DPGVDSDDPR NKHKFKVHSY NSPTFCDHCG SLLYGLYHQG 130 140 150 160 170 180 MKCGACDMNV HKRCQKSVPN LCGADHTERR GRIKVKAEVI GNKLQVTVAE AKNLIPMDPN 190 200 210 220 230 240 GLSDPFVKLK LIPDQKRETK KKTRTIKGSL NPTWGESFDF NLEDTDRNRR LLVEVWDWDR 250 260 270 280 290 300 ATRNDFMGAL SFGISELMKA GVDAWYKLLG QEEGEYYNVP AIAETESIDE LTSNIKKLPM 310 320 330 340 350 360 PTQEHVKPQN SNSMSGMGVV RASDFNFLSV LGKGSFGKVM LAEKKGTDEL YAIKILKKDV 370 380 390 400 410 420 IIQDDDVECT MTEKRVLGLP SKPAFLTALH SCFQTMDRLF FVMEFVNGGD LMFQIQKVGK 430 440 450 460 470 480 FREPHAVFYA AEIAVGLFYL HSQGVIYRDL KLDNVLVDAE GHIKIADFGM CKEHMNEGDT 490 500 510 520 530 540 TRTFCGTPDY IAPEIVAYQP YGKAVDWWAF GVLLYEMLAG QPPFDGEDED ELFQSIMEHV 550 560 570 580 590 600 PSYPKSMSRE SVTMCKGFLT KHPGKRLGSG PTGEQDIREH QFFRRIDWEK LANREIQPPF 610 620 630 640 650 VPSVRNPRAA ENFDPYFTKI PCALTPTDKL IIMNIQDEFQ GFTFVNEVFD GYVRSLPS

References

[1]	"Molecular cloning and characterization of protein kinase C from the sea urchin Lytechinus pictus." Rakow T.L., Shen S.S. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02967 mRNA. Translation: AAA03447.1.
3D structure databases
HSSP	HSSP built from PDB template 1TBN based on UniProtKB P05697.
SMR	Q25378. Positions 86-150.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.13. 3099.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR020477. C2_region. IPR020454. DAG/PE_bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014375. Protein_kinase_C_a/b/g. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000550. PKC_alpha. 1 hit.
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPC1_LYTPI

Accession

Primary (citable) accession number: Q25378

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)