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Protein

Protein kinase C

Gene

PKC1

Organism
Lytechinus pictus (Painted sea urchin)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.By similarity
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei354 – 3541ATPPROSITE-ProRule annotation
Active sitei449 – 4491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 7751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri92 – 14251Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi331 – 3399ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C (EC:2.7.11.13)
Gene namesi
Name:PKC1
OrganismiLytechinus pictus (Painted sea urchin)
Taxonomic identifieri7653 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaEchinozoaEchinoideaEuechinoideaEchinaceaTemnopleuroidaToxopneustidaeLytechinus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658Protein kinase CPRO_0000055729Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ25378.
SMRiQ25378. Positions 28-280, 323-649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini164 – 25188C2PROSITE-ProRule annotationAdd
BLAST
Domaini325 – 583259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini584 – 65471AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 7751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri92 – 14251Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q25378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSTLEMKG FARRGALRQK NVHEIKNHKF IPRFFKQPTF CSHCKDFIWG
60 70 80 90 100
FGKQGFQCKV CSFVVHKRCH EFVTFQCPGL DPGVDSDDPR NKHKFKVHSY
110 120 130 140 150
NSPTFCDHCG SLLYGLYHQG MKCGACDMNV HKRCQKSVPN LCGADHTERR
160 170 180 190 200
GRIKVKAEVI GNKLQVTVAE AKNLIPMDPN GLSDPFVKLK LIPDQKRETK
210 220 230 240 250
KKTRTIKGSL NPTWGESFDF NLEDTDRNRR LLVEVWDWDR ATRNDFMGAL
260 270 280 290 300
SFGISELMKA GVDAWYKLLG QEEGEYYNVP AIAETESIDE LTSNIKKLPM
310 320 330 340 350
PTQEHVKPQN SNSMSGMGVV RASDFNFLSV LGKGSFGKVM LAEKKGTDEL
360 370 380 390 400
YAIKILKKDV IIQDDDVECT MTEKRVLGLP SKPAFLTALH SCFQTMDRLF
410 420 430 440 450
FVMEFVNGGD LMFQIQKVGK FREPHAVFYA AEIAVGLFYL HSQGVIYRDL
460 470 480 490 500
KLDNVLVDAE GHIKIADFGM CKEHMNEGDT TRTFCGTPDY IAPEIVAYQP
510 520 530 540 550
YGKAVDWWAF GVLLYEMLAG QPPFDGEDED ELFQSIMEHV PSYPKSMSRE
560 570 580 590 600
SVTMCKGFLT KHPGKRLGSG PTGEQDIREH QFFRRIDWEK LANREIQPPF
610 620 630 640 650
VPSVRNPRAA ENFDPYFTKI PCALTPTDKL IIMNIQDEFQ GFTFVNEVFD

GYVRSLPS
Length:658
Mass (Da):74,871
Last modified:November 1, 1996 - v1
Checksum:i74B5A27A49C835A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02967 mRNA. Translation: AAA03447.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02967 mRNA. Translation: AAA03447.1.

3D structure databases

ProteinModelPortaliQ25378.
SMRiQ25378. Positions 28-280, 323-649.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of protein kinase C from the sea urchin Lytechinus pictus."
    Rakow T.L., Shen S.S.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiKPC1_LYTPI
AccessioniPrimary (citable) accession number: Q25378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.