Reviewed, UniProtKB/Swiss-Prot Q25263 (CYAB_LEIDO)

Names and origin

Protein names

Recommended name:
    Receptor-type adenylate cyclase B
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase
    Adenylyl cyclase

Gene names

Name:

RAC-B

Organism

Leishmania donovani

Taxonomic identifier

5661 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	1331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Could act as a receptor for a unknown ligand.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Developmental stage	Expressed in the insect stage (promastigote) but not in the mammalian host stage of the parasite life cycle.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase Receptor
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1331	1331	Receptor-type adenylate cyclase B		PRO_0000195735
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Potential
	Topological domain	55 – 898	844	Extracellular Potential
	Transmembrane	899 – 919	21	Potential
	Topological domain	920 – 1331	412	Cytoplasmic Potential
	Domain	940 – 1094	155	Guanylate cyclase
Sites
	Metal binding	945	1	Magnesium By similarity
	Metal binding	988	1	Magnesium By similarity
Amino acid modifications
	Glycosylation	255	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Glycosylation	558	1	N-linked (GlcNAc...) Potential
	Glycosylation	574	1	N-linked (GlcNAc...) Potential
	Glycosylation	657	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q25263-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5FC3AA22DC1E5072	FASTA	1,331	144,163
	10 20 30 40 50 60 MYADATHPRR ACWCGAGGVS GCVRQRHAYR CSRLLAGVLL IVGALTLTLA VSTVPAAWAA 70 80 90 100 110 120 GAVASSDEPV YLLNAMYSLS DYNAKHAKAL WLGIDSALHA VGYTAARGRP IKIIEPDPTD 130 140 150 160 170 180 DLSDIVAVVL KALKDYPTLL GVIGPYSDTR LGAVLISPEI QNSGLMFLGP FTGSSSMRAW 190 200 210 220 230 240 NENLYFMRAE PRLEIMAMVK HIANTFRARR TAFMYLTGEQ YGSFEHKSLV ELMTSLSLDP 250 260 270 280 290 300 PAVYSASYST STAVNMTAFD AMADTRPQVI IIWGIPAGQV EELLKVVLTD PRTSSAYIMP 310 320 330 340 350 360 SFALQQMTFQ VYYDLAMAGK LTPVDGQIIS SATSFPLTEP ASVHLRVFRA QMGEYMVKTG 370 380 390 400 410 420 RVDASLWADE AKAVQQYGPW EHEASSSDSA AYVNNFFNEH PCVTQLMIAG WISGSLIAQT 430 440 450 460 470 480 LAEENRIANR TAYRQYMFSQ QRYIVGEDFV LGDYGGPCNG VAEFLGAVCY CNQGGHSAVL 490 500 510 520 530 540 SRLDRAVWTV ITESGVSFTQ KNCYSDGTTL PRPLNFLTLI FAEHPLLAQV GLTFKTSIST 550 560 570 580 590 600 LVAYLQYNAS PVNAATVNVT DTTPQALHDA VTTNYTTDVV VGVTVKGMNV DGYLVPSPIH 610 620 630 640 650 660 PRPHLVELLR NYVYLMPTLE QQMFVLYAKL SAVRGVTSID SAVHMILHGY ASDEVANITA 670 680 690 700 710 720 VLRKSAATFN YDNPTVTAVP STKTVGSALA HGQINFVLAV TAADVADIVD FLVEEKTSIV 730 740 750 760 770 780 VIVFDDLVIQ YPTLVTALKS KPASVQARVI TFTNLPLWSD TSESAHAASK LLTVFHDALP 790 800 810 820 830 840 DPSQHTPGFL SAVLTGSFCA SMRRLADSVH STSLTDMVYR EGSVTTFAEP FGRFQWGCTT 850 860 870 880 890 900 TPTDRFCVYH NYGAQGIVML SVQRMLDPTV PQLSSPMTPT MDYRPRQRSH ALTPAQRGGA 910 920 930 940 950 960 IAGIALLTVI LLAVAGLALY CCMDNRNNDA APKDGDEPVT LLFTDIESST ALWAALPQLM 970 980 990 1000 1010 1020 SDAIAAHHRV IRQLVKKYGC YEVKTIGDSF MIACRSAHSA VSLACEIQTK LLKHDWGTEA 1030 1040 1050 1060 1070 1080 LDRAYREFEL ARVDTLDDYE PPTARLSEEE YAALWCGLRV RVGIHTGLTD IRYDEVTRGY 1090 1100 1110 1120 1130 1140 DYYGDTSNMA ARTEAVANGG QVVATEAAWW ALSNDERAGI AHTAMGPQGL RGVPFAVEMF 1150 1160 1170 1180 1190 1200 QLNAVPGRRH AALRTEIEAI LPDDTATDTA SSAAGALLSS VGTINGPAAG IAFVLASCFA 1210 1220 1230 1240 1250 1260 PYPVAQRVRE LQPLLSKWGV GAPPRSRLVS EEDYCQGLMN RLAIRIATVS QARCPVGNNG 1270 1280 1290 1300 1310 1320 AAVDLDVQHA GTAEVMNPLL GEGSFISDGA RARHSGLTAV PPSAEPSAMR MRRVGRKVPE 1330 RPTVCNVRGA H

References

[1]	"A family of putative receptor-adenylate cyclases from Leishmania donovani." Sanchez M.A., Zeoli D., Klamo E.M., Kavanaugh M.P., Landfear S.M. J. Biol. Chem. 270:17551-17558(1995) [PubMed: 7615561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MHOM/SD/62/1S.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U17043 Genomic DNA. Translation: AAA74999.1.
PIR	T18310.
3D structure databases
HSSP	HSSP built from PDB template 1FX2 based on UniProtKB Q99279.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.6.1.1. 882.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYAB_LEIDO

Accession

Primary (citable) accession number: Q25263

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)