Reviewed, UniProtKB/Swiss-Prot Q251P8 (FTHS1_DESHY)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase 1
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase 1
      Short name=FHS 1
      Short name=FTHFS 1

Gene names

Name:	fhs1
Ordered Locus Names:	DSY0205

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase 1 HAMAP MF_01543		PRO_0000293034
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q251P8-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 3ED979494B7BE038	FASTA	556	60,058
	10 20 30 40 50 60 MKTDIEIAQE ATMKPITEIA QGLDLLEDEI ELYGKYKAKV NFSAWERLKD KPDAKLILVT 70 80 90 100 110 120 AINPTPAGEG KTTTTVGLGQ AMSKIGKNAM IALREPSLGP CFGVKGGAAG GGYAQVVPME 130 140 150 160 170 180 DINLHFTGDF HAITSTHNLL AALLDNHIQQ GNLLNIDPRQ IVFRRVMDMN DRALRKIVIG 190 200 210 220 230 240 LGGRTEGIPR ENGFDITVAS EIMAILCLAK DLMDLKERFG RIVVAYTYDG KAITAHDLEA 250 260 270 280 290 300 EGAMALLMKD AIKPNLVQTL ENTPVFIHGG PFANIAHGCN SVVATRMAMK LADYVITEAG 310 320 330 340 350 360 FGADLGAEKF YDLKCRFAEL KPAATVIVAT VRALKMNGGV AKEDLGPENL EALAKGIVNL 370 380 390 400 410 420 EKHIENIGKF GVPAVVAINR FPTDTDAELE FVAERCRQLG AEFALSEVFT KGGEGGIELA 430 440 450 460 470 480 KAVLNIVDNK ESNFHVLYEL DLPIAKKIET ICKEVYGADG VNFTKEALTS MKKYEELGYG 490 500 510 520 530 540 QLPICMAKTQ YSLTDDQNVL GRPSGFTITV RELRLSAGAG FLVAITGAIM TMPGLPKRPA 550 ALRMDIDAAG RITGLF

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008230 Genomic DNA. Translation: BAE81994.1.
RefSeq	YP_516438.1.
3D structure databases
SMR	Q251P8. Positions 4-554.
ModBase	Search...
Protein-protein interaction databases
STRING	Q251P8.
Genome annotation databases
GeneID	3953868.
GenomeReviews	Gene locus DSY0205 in contig AP008230_GR.
KEGG	dsy:DSY0205.
NMPDR	fig|138119.3.peg.285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q251P8.
OMA	GMKGGAT.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY0205-MON.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS1_DESHY

Accession

Primary (citable) accession number: Q251P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)