Q250Q8 (SYE_DESHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	DSY0445

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001893
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	249 – 253	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Metal binding	105	1	Zinc By similarity
	Metal binding	107	1	Zinc By similarity
	Metal binding	132	1	Zinc By similarity
	Metal binding	134	1	Zinc By similarity
	Binding site	252	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q250Q8 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: F2B01FF825543AEA	FASTA	488	54,926
	10 20 30 40 50 60 MLKVRFAPSP TGPLHIGGAR SALFNYLLAR KEDGVFIVRS EDTDLERSSR ESEHNIMEAL 70 80 90 100 110 120 RWLNIQWDEG IEVGGDNGPY RQTERLALYQ EYTDRLLASG DAYYCYCSEE ELEQERQDLM 130 140 150 160 170 180 AKGETPRYLG KCRHLSAAER QTYEAAGRKP VVRFRVPEGR QILINDRVRG EVVFDSDGIG 190 200 210 220 230 240 DYVIVKSDGI PTYNFAVVID DTTMNITHVI RGEEHLSNTP RQVLIYQALG LPTPEFAHIS 250 260 270 280 290 300 LILNTEGKKM SKRDGDTAVI DYQAKGYLPE AVVNFIALMG WSPPGEEEFF TLEEMTQAFS 310 320 330 340 350 360 LERVSKSPAV FDLNKLNYMN AHYIKQADPE RLTDLAVPYL REMGAIPQGT LSEEERAWVT 370 380 390 400 410 420 HYVQAIINHL SYMAQVKDFV HYVQGGEAPT PEGEALEILQ GEQVPAVLDL FVEKLKSLEA 430 440 450 460 470 480 IRVDTVKPLF KQITKETKLG GKQVFMPIRI ALTGQMHGPE LYDIVPLLGL ENVLSRLAGT KALLAGSR

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y51.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008230 Genomic DNA. Translation: BAE82234.1.
RefSeq	YP_516678.1. NC_007907.1.
3D structure databases
ProteinModelPortal	Q250Q8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q250Q8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3955455.
GenomeReviews	Gene locus DSY0445 in contig AP008230_GR.
KEGG	dsy:DSY0445.
NMPDR	fig|138119.3.peg.525.
PATRIC	21669057. VBIDesHaf65307_0501.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	Q250Q8.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY0445-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_DESHY

Accession

Primary (citable) accession number: Q250Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program