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Q24ZG8 (ASSY_DESHY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:DSY0785
OrganismDesulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]
Taxonomic identifier138119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263921

Regions

Nucleotide binding15 – 239ATP By similarity

Sites

Binding site421ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site931Citrulline By similarity
Binding site981Citrulline By similarity
Binding site1231ATP; via amide nitrogen By similarity
Binding site1251Aspartate By similarity
Binding site1291Aspartate By similarity
Binding site1291Citrulline By similarity
Binding site1301Aspartate By similarity
Binding site1331Citrulline By similarity
Binding site1821Citrulline By similarity
Binding site1911Citrulline By similarity
Binding site2671Citrulline By similarity
Binding site2791Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q24ZG8 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: DE018F6449827761

FASTA40945,535
        10         20         30         40         50         60 
MLRKEVIPMK KVVLAYSGGL DTSIIIPWLK ENYGYEVIAM AADLGQGEEL EPLHEKAIKS 

        70         80         90        100        110        120 
GASKLYIEDL QEEFVTDFIY PTLKAGAVYE GKYLLGTSFA RPLIAQRLVE IAAKEGAVAI 

       130        140        150        160        170        180 
AHGATGKGND QVRFELAVKA LNPDLEIIAP WRIWDIKSRE DAIDYAVERG IPVPVTKDRP 

       190        200        210        220        230        240 
YSMDRNVWHL SHEGGDLEDP WNEPKKDLYL LGVSPEDAPD EAEYLELDFE QGIPVSLNGE 

       250        260        270        280        290        300 
KLGPVQLLET LNEVGGKHGI GIVDMVENRL VGMKSRGVYE TPGGTILYTA HQALEHLTLD 

       310        320        330        340        350        360 
RLTLHYKEQI ALKYAELVYD GVWHSPLREA LDAFVDVTQK NVTGTVRLKL YKGNCSLAGV 

       370        380        390        400 
KSPYSLYSEE FATFGRDGVY NQKDAEGFIN LFGLPLKVRA LMEKKSGLR 

« Hide

References

[1]"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195."
Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H.
J. Bacteriol. 188:2262-2274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y51.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008230 Genomic DNA. Translation: BAE82574.1.
RefSeqYP_517018.1. NC_007907.1.

3D structure databases

ProteinModelPortalQ24ZG8.
SMRQ24ZG8. Positions 11-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING138119.DSY0785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE82574; BAE82574; DSY0785.
GeneID3954984.
KEGGdsy:DSY0785.
PATRIC21669835. VBIDesHaf65307_0887.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycDHAF138119:GHT5-810-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_DESHY
AccessionPrimary (citable) accession number: Q24ZG8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways