Q24YU1 (KATG_DESHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	DSY1012

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Catalase-peroxidase HAMAP MF_01961		PRO_0000354767
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q24YU1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 1345E2AFC4F51603	FASTA	730	80,533
	10 20 30 40 50 60 MEEKKCPVTG HTQHTPTGGG TKNKDWWPNQ LNLNILHQNS ALGNPMDPDF NYAEEFKKLD 70 80 90 100 110 120 LAAVKKDLYA LMTDSQDWWP ADYGHYGPLF IRMAWHSAGT YRLNDGRGGA GDGTQRFAPL 130 140 150 160 170 180 NSWPDNVNLD KARRLLWPIK QKYGKKISWA DLMVLAGNCA LESMGFKTFG FAGGREDVWE 190 200 210 220 230 240 PQEDIYWGSE GEWLGDQRYS GDRDLENPLA AVQMGLIYVN PEGPNGQPSV LASGRDVRDT 250 260 270 280 290 300 FKRMAMNDEE TVALVAGGHT FGKCHGAGPA SHVGPEPEAA PLEEQGLGWK STFRSGKGGD 310 320 330 340 350 360 TIGSGIEGAW KPNPTTWDMG YLETLFKYDW DLVKSPAGAW QWVPTDPAAA DTVQDAHDPA 370 380 390 400 410 420 KRHAPMMTTA DLSLRMDPIF GPIAKRFRDN PEEFADAFAR AWFKLTHRDM GPRSRYLGPE 430 440 450 460 470 480 VPEEELIWQD PVPPVDHELI DEQDIADLKA KLLASGLSVS QLVSTAWASA STFRGSDKRG 490 500 510 520 530 540 GANGARIRLA PQKDWEINQP AQLAEVLAAL EGIQTEFNSS QSGTKKVSLA DLIVLGGAAA 550 560 570 580 590 600 IEQAARNAGH TLVLPFTPGR TDATQEQTEI YSFAVMEPKA DGFRNYLKGK SSAPAEEMLV 610 620 630 640 650 660 DRAQLLTLTA PEMTVLLGGL RVLNANYGQS KHGVFTQRPE TLTNDFFVNL LDMGTEWKAV 670 680 690 700 710 720 SEEKALYEGR DRATGELKWT GTRVDLVFGS NSQLRALAEV YACSDSQDKF LQDFVSAWNK 730 VMNADRFDLA

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y51.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008230 Genomic DNA. Translation: BAE82801.1.
RefSeq	YP_517245.1. NC_007907.1.
3D structure databases
ProteinModelPortal	Q24YU1.
SMR	Q24YU1. Positions 20-730.
ModBase	Search...
Protein-protein interaction databases
STRING	Q24YU1.
Protein family/group databases
PeroxiBase	3653. DhaCP01_Y51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3953187.
GenomeReviews	Gene locus DSY1012 in contig AP008230_GR.
KEGG	dsy:DSY1012.
NMPDR	fig|138119.3.peg.1282.
PATRIC	21670328. VBIDesHaf65307_1131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	Q24YU1.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY1012-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_DESHY

Accession

Primary (citable) accession number: Q24YU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program