Reviewed, UniProtKB/Swiss-Prot Q24XQ1 (THIM_DESHY)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	DSY1402

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000336553
Sites
	Binding site	44	1	Substrate; via amide nitrogen By similarity
	Binding site	120	1	ATP By similarity
	Binding site	166	1	ATP By similarity
	Binding site	193	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q24XQ1-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 049C1D24411E604E	FASTA	267	27,836
	10 20 30 40 50 60 MKEKLTEALQ ALKQKTPLVH AITNVVTVND CANSLLAVGA SPAMCEAADE VFEFSQLAGS 70 80 90 100 110 120 LYLNLGTLTK EQEMAAYLAI RGATLKGIPV ILDPVACGAI PRKKATIERL GHFGRFTVIK 130 140 150 160 170 180 GNLGEVKALA GLAARVRGVD SLDEGNDGLE ACQSLARAYG CVVAATGKVD IVADGQRACL 190 200 210 220 230 240 IENGTEMLTR ITGAGCMAGA LVAGFCGAYE DAFGATVAAL LTMSLAGELA QETSGGELPG 250 260 TFRAHLIDQL SLVDEALIEK RGRVQWL

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008230 Genomic DNA. Translation: BAE83191.1.
RefSeq	YP_517635.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3954052.
GenomeReviews	Gene locus DSY1402 in contig AP008230_GR.
KEGG	dsy:DSY1402.
NMPDR	fig|138119.3.peg.1674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q24XQ1.
OMA	Q24XQ1. MANEAPE.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY1402-MON.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_DESHY

Accession

Primary (citable) accession number: Q24XQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)