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Q24TH1 (SYI_DESHY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:DSY2882
OrganismDesulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]
Taxonomic identifier138119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium

Protein attributes

Sequence length922 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 922922Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000022059

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif594 – 5985"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8921Zinc By similarity
Metal binding8951Zinc By similarity
Metal binding9121Zinc By similarity
Metal binding9151Zinc By similarity
Binding site5531Aminoacyl-adenylate By similarity
Binding site5971ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q24TH1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: EE79E4B06F05EABC

FASTA922104,696
        10         20         30         40         50         60 
MDYRNTLNLP ETDFPMRGNL PQREPEILQK WEEEDIYATV QKARAGRPKF VLHDGPPYAN 

        70         80         90        100        110        120 
GDIHLGHALN KVIKDIIVKY KTMAGFDAPY VPGWDTHGLP IEQQVIKKLG VNRHAVSVVE 

       130        140        150        160        170        180 
FRRMCKEYAK KYISIQKEQF KRLGVRGDWK NPYLTLEKEY EAAQIGVFGK MARKKYIYKG 

       190        200        210        220        230        240 
LKPVYWCPSC ETALAEAEIE YAEKTSHAIY VKFPVKEGKG VLTDENTFVI IWTTTPWTLP 

       250        260        270        280        290        300 
ANLAITLHEE FSYVQVQVEK EHWLVAEGML ESLRSLWNLE LPVEKRFVGK ELEGVICKHP 

       310        320        330        340        350        360 
FIERDSVLIL GEHVTLEAGT GCVHTAPGHG EEDFNVGKKY GLPVLCPVDH QGKFTAEGGA 

       370        380        390        400        410        420 
YAGMKVDKAN PVIIEDLKNL HALVHEDKIK HSYAHCWRCN NPIIYRATEQ WFASIDGFRK 

       430        440        450        460        470        480 
AALEEIDKVQ WIPSWGKDRI YNMIADRGDW CISRQRTWGV PIPIFYCEDC GKEIISDETI 

       490        500        510        520        530        540 
AKVQEIFREE GSDAWFLRPA AELLPEGFTC ACGGKSFRKE TDIMDVWFDS GTSHTSVLME 

       550        560        570        580        590        600 
RKELAWPADL YMEGSDQHRG WFNSSLSTSV AAYGKAPYKA VLTHGFLVDE KGRKMSKSLG 

       610        620        630        640        650        660 
NGVDPLQVTK EMGADILRLW VCAADYKNDV AVSPRIMKQM SEAYRKIRNT LRFLLSNLND 

       670        680        690        700        710        720 
FDPAKDRVAY KDLPEIDRWA LLQLGKVTQR VLQGYEKYEF HWVYHSVHNF CAVELSAIYL 

       730        740        750        760        770        780 
DIVKDRLYVE GKNSTLRRAS QTVLYDVLNA LVRLMAPVLT YTADEIWPYV PGVPAGSHVQ 

       790        800        810        820        830        840 
TEEMPEALPQ WLDEALEKKW DTLLAVRSEV TKALEKARQD KLINHPLTAQ VDLYPNAELE 

       850        860        870        880        890        900 
GFLRGIPNLS EIFIVSAVQL HSAGEEKPEG LSMAEDLAGF GIAVNSAAGE KCERCWIYDT 

       910        920 
GVGENQEHPT LCPRCASVVS HL 

« Hide

References

[1]"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195."
Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H.
J. Bacteriol. 188:2262-2274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y51.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008230 Genomic DNA. Translation: BAE84671.1.
RefSeqYP_519115.1. NC_007907.1.

3D structure databases

ProteinModelPortalQ24TH1.
SMRQ24TH1. Positions 1-922.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING138119.DSY2882.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE84671; BAE84671; DSY2882.
GeneID3955638.
KEGGdsy:DSY2882.
PATRIC21674594. VBIDesHaf65307_3232.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycDHAF138119:GHT5-2946-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_DESHY
AccessionPrimary (citable) accession number: Q24TH1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries