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Q24SP5 (SYL_DESHY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DSY3158
OrganismDesulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]
Taxonomic identifier138119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334749

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif583 – 5875"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5861ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q24SP5 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 9ABC3B33C891064E

FASTA82794,532
        10         20         30         40         50         60 
MQEKYLFSEI EPKWQKKWVD RKDYQAEEHS DKPKFYALAM FPYPSGNLHM GHVRNYSIVD 

        70         80         90        100        110        120 
VIARFKRMRG YNVLHPIGWD SFGLPAENAA IKNQTPPAEW TWKNIANMKR QLQEMGISYD 

       130        140        150        160        170        180 
WEREVTTCHP DYYKFTQWIF LEFYKHGLVY KKKAGVNWCP SCATVLANEQ VVDGACERCD 

       190        200        210        220        230        240 
TAVTKKDLEQ WFFKITDYAQ VLLDDLEKLP GWPDKVKTMQ KNWIGRSEGA EVEFDLENHG 

       250        260        270        280        290        300 
DKIRVYTTRV DTIFGVSYVV LAPEHPLVQK LIAGTEYEND VQAFIERMKG LNEIARTSTE 

       310        320        330        340        350        360 
TEKEGLFTGA YCINPYSGEK VPIWIANYVL FEYGTGAVMG VPAHDERDFE FAGKYKLPIK 

       370        380        390        400        410        420 
TVILPEGTPV EEKDTPLQAA FVEEGMMVNS GEYDGLKNVE AWEKMCDKAE HDGFGERKVN 

       430        440        450        460        470        480 
FRLRDWLISR QRYWGAPIPM IYCDHCGIVP VPQDQLPVML PDDVVFKAGE NPLTTSESFK 

       490        500        510        520        530        540 
QTICPTCGGK ARRETDTMDT FMCSSWYFLR YTDPHNAQLP FAKEAADHWM NVDQYVGGVE 

       550        560        570        580        590        600 
HAILHLLYSR FFTKALRDFG YLKVDEPFAN LLTQGMVCLG GAKMSKSKGN VVSPEEIISK 

       610        620        630        640        650        660 
YGADTARLFI LFAAPPERDL EWNDQGVEGC YRFLNRVWRL AAQYEEVLKT TGAGANEFGE 

       670        680        690        700        710        720 
LDKAAKDMRR QTHQTIQRVT SDVGARFNFN TAVSAIMELV NALYLYKEQP QVNLAVAREA 

       730        740        750        760        770        780 
LESILILLAP FAPHITEEIW SELGHEDSIH SREWPQVDEE ALVQEEVTVV LQINGKVKER 

       790        800        810        820 
IQVPAQISAA ELEAQVRQLP RLGEWTQGKQ ILKIVTVPGK LVNVVVK 

« Hide

References

[1]"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195."
Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H.
J. Bacteriol. 188:2262-2274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y51.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008230 Genomic DNA. Translation: BAE84947.1.
RefSeqYP_519391.1. NC_007907.1.

3D structure databases

ProteinModelPortalQ24SP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING138119.DSY3158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE84947; BAE84947; DSY3158.
GeneID3956540.
KEGGdsy:DSY3158.
PATRIC21675200. VBIDesHaf65307_3532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycDHAF138119:GHT5-3226-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DESHY
AccessionPrimary (citable) accession number: Q24SP5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries