Reviewed, UniProtKB/Swiss-Prot Q24PT9 (ALLB_DESHY)

Names and origin

Protein names

Recommended name:
    Allantoinase
    EC=3.5.2.5
Alternative name(s):
    Allantoin-utilizing enzyme

Gene names

Name:	allB
Ordered Locus Names:	DSY4164

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring By similarity.
Catalytic activity	(S)-allantoin + H2O = allantoate. HAMAP MF_01645
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1. HAMAP MF_01645
Subunit structure	Homotetramer By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity.
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	allantoin catabolic process Inferred from electronic annotation. Source: HAMAP purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	allantoinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Allantoinase HAMAP MF_01645		PRO_0000317673
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	148	1	Zinc 1; via carbamate group By similarity
	Metal binding	148	1	Zinc 2; via carbamate group By similarity
	Metal binding	184	1	Zinc 2 By similarity
	Metal binding	240	1	Zinc 2 By similarity
	Metal binding	313	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	148	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q24PT9-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: E96C55461047E725	FASTA	449	49,228
	10 20 30 40 50 60 MSHYDLILRN GNVVCPDGVR KADIAVSDGK IVLIAEEIPG DAKEVIDAAG KHVFPGITDG 70 80 90 100 110 120 HVHFNDPGRT EWETISTGSS ALAAGGGVAY FDMPLNCSPC TLDAVNFNNK LAVAQKDSLV 130 140 150 160 170 180 DYGFWGGLTS ANLDKLDELA ECGVIGFKAF ACHSGIDEFP RMDDYTALVG MEKLAKLGLP 190 200 210 220 230 240 LMVHCENAEI TKELTELSLA NNRTGVRDYF AARPPITEIE NVSRMITFAE ETGCKLIIAH 250 260 270 280 290 300 ISTAKAVDLV AQARARGVDV YCETIGHYLY LTGDDVERLG TVAKCSPPIR DGENQLQMWG 310 320 330 340 350 360 RLFNDNIAFV SSDHSPCDPK LKNGEFMRVW GGISACQTTL QGLLTHAYHD RKFPLVKIAQ 370 380 390 400 410 420 LTAQHVNEIF KIKGKGQINL GYDADFALVD LNHEFTLQAE DLFYKHKVSP YVGDRFRGSV 430 440 SQTILRGTTI YKDGKIVSQP IGKHLRPHQ

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008230 Genomic DNA. Translation: BAE85953.1.
RefSeq	YP_520397.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q24PT9.
Genome annotation databases
GeneID	3954326.
GenomeReviews	Gene locus DSY4164 in contig AP008230_GR.
KEGG	dsy:DSY4164.
NMPDR	fig|138119.3.peg.4339.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q24PT9.
OMA	FEAWGGI.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY4164-MON.
Family and domain databases
HAMAP	MF_01645. [Tree]
InterPro	IPR017593. Allantoinase. IPR006680. Amidohydro_1. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03178. allantoinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALLB_DESHY

Accession

Primary (citable) accession number: Q24PT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)