Reviewed, UniProtKB/Swiss-Prot Q24MK1 (SYN_DESHY)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	DSY4952

Organism

Desulfitobacterium hafniense (strain Y51) [Complete proteome] [HAMAP]

Taxonomic identifier

138119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051390

Sequences

	Sequence		Length	Mass (Da)
	Q24MK1-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 62D5C3F18AD4E9B3	FASTA	463	53,279
	10 20 30 40 50 60 MENTLIKSIY RDTPAYLNQE LQISGWVRTL RVSKSFGFIE INDGSFFKGI QVVFEEDLNN 70 80 90 100 110 120 FEEIAKVATG SSLIIKGLLV ESPGAKQPFE LKAQSIMIEG ACSGDYPLQK KRHSFEYLRT 130 140 150 160 170 180 IAHLRPRTNT FAAVFRVRSL VAYAIHKFFQ DKGFVYIHTP IITGSDAEGA GEMFRVTTLD 190 200 210 220 230 240 LDALPRTEEG RIDYAKDFFG KETNLTVSGQ LNAETYCMAF RNVYTFGPTF RAENSNTARH 250 260 270 280 290 300 AAEFWMIEPE IAFADLQDDM ELAEEMMKYL ISYVLEHAPE EMAFFNEFVD KTLFDRLDNI 310 320 330 340 350 360 VNSDFGRLTY TEAIEILQKE KERFEFPVEW GADLQTEHER FLTEQIFKKP VFVVDYPKDI 370 380 390 400 410 420 KAFYMRLNDD QKTVAAMDLL VPGVGEIIGG SQREERLDYL EKRMAELGLH KEDYSWYLDL 430 440 450 460 RKYGGTRHAG YGLGFERVIM YLTGISNIRD VVAFPRTVKN ADF

References

[1]

"Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195." Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K., Inatomi K., Furukawa K., Inui M., Yukawa H. J. Bacteriol. 188:2262-2274(2006) [PubMed: 16513756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008230 Genomic DNA. Translation: BAE86741.1.
RefSeq	YP_521185.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q24MK1.
Genome annotation databases
GeneID	3957891.
GenomeReviews	Gene locus DSY4952 in contig AP008230_GR.
KEGG	dsy:DSY4952.
NMPDR	fig|138119.3.peg.4968.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q24MK1.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	DHAF138119:DSY4952-MON.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_DESHY

Accession

Primary (citable) accession number: Q24MK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)