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Q24K09

- DNMT1_BOVIN

UniProt

Q24K09 - DNMT1_BOVIN

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Protein

DNA (cytosine-5)-methyltransferase 1

Gene

DNMT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9 By similarity.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi351 – 3511ZincBy similarity
Metal bindingi354 – 3541ZincBy similarity
Metal bindingi412 – 4121ZincBy similarity
Metal bindingi416 – 4161ZincBy similarity
Sitei507 – 5071Important for activityBy similarity
Active sitei1223 – 12231PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri643 – 68947CXXC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB
  3. methyl-CpG binding Source: Ensembl
  4. RNA binding Source: Ensembl
  5. zinc ion binding Source: Ensembl

GO - Biological processi

  1. cellular response to amino acid stimulus Source: Ensembl
  2. chromatin modification Source: UniProtKB-KW
  3. gene silencing Source: Ensembl
  4. maintenance of DNA methylation Source: UniProtKB
  5. negative regulation of histone H3-K9 methylation Source: Ensembl
  6. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  7. positive regulation of gene expression Source: Ensembl
  8. positive regulation of histone H3-K4 methylation Source: Ensembl
  9. regulation of cell proliferation Source: Ensembl
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiREACT_207393. PRC2 methylates histones and DNA.
REACT_214253. NoRC negatively regulates rRNA expression.

Protein family/group databases

REBASEi7406. M.BtaDnmt1AP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
Short name:
Dnmt1
Gene namesi
Name:DNMT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 7

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. pericentric heterochromatin Source: Ensembl
  3. replication fork Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16111611DNA (cytosine-5)-methyltransferase 1PRO_0000239845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6,N6-dimethyllysine; by EHMT2By similarity
Modified residuei133 – 1331PhosphoserineBy similarity
Modified residuei142 – 1421N6-methyllysine; by SETD7By similarity
Modified residuei143 – 1431Phosphoserine; by PKB/AKT1By similarity
Modified residuei152 – 1521PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei160 – 1601N6-acetyllysineBy similarity
Modified residuei188 – 1881N6-acetyllysineBy similarity
Modified residuei257 – 2571N6-acetyllysineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei507 – 5071PhosphoserineBy similarity
Modified residuei711 – 7111PhosphoserineBy similarity
Modified residuei729 – 7291PhosphoserineBy similarity
Modified residuei746 – 7461N6-acetyllysineBy similarity
Modified residuei888 – 8881N6-acetyllysineBy similarity
Modified residuei954 – 9541N6-acetyllysineBy similarity
Modified residuei958 – 9581N6-acetyllysineBy similarity
Modified residuei972 – 9721N6-acetyllysineBy similarity
Modified residuei1051 – 10511N6-acetyllysineBy similarity
Modified residuei1108 – 11081N6-acetyllysineBy similarity
Modified residuei1110 – 11101N6-acetyllysineBy similarity
Modified residuei1112 – 11121N6-acetyllysineBy similarity
Modified residuei1114 – 11141N6-acetyllysineBy similarity
Modified residuei1118 – 11181N6-acetyllysineBy similarity
Modified residuei1346 – 13461N6-acetyllysineBy similarity
Modified residuei1412 – 14121N6-acetyllysineBy similarity

Post-translational modificationi

Sumoylated.By similarity
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1346 and Lys-1412 by SIRT1 increases methyltransferase activity By similarity.By similarity
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability By similarity.By similarity
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.By similarity
Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ24K09.

Interactioni

Subunit structurei

Homodimer. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B By similarity. Interacts with the PRC2/EED-EZH2. complex. Interacts with UBC9 and BAZ2A/TIP5 By similarity. Binds to CSNK1D. Interacts with BAZ2A/TIP5. Binds to CSNK1D. Interacts with UHRF1; promoting its recruitment to hemimethylated DNA. Interacts with USP7, promoting its deubiquitination By similarity.By similarity

Protein-protein interaction databases

BioGridi158481. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ24K09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 10689DMAP-interactionAdd
BLAST
Domaini752 – 877126BAH 1PROSITE-ProRule annotationAdd
BLAST
Domaini969 – 1097129BAH 2PROSITE-ProRule annotationAdd
BLAST
Repeati1106 – 110721
Repeati1108 – 110922
Repeati1110 – 111123
Repeati1112 – 111324
Repeati1114 – 111525; approximate
Domaini1136 – 1595460SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 334334Interaction with the PRC2/EED-EZH2 complexBy similarityAdd
BLAST
Regioni1 – 148148Interaction with DNMT3ABy similarityAdd
BLAST
Regioni1 – 120120Interaction with DMAP1By similarityAdd
BLAST
Regioni149 – 21668Interaction with DNMT3BBy similarityAdd
BLAST
Regioni163 – 17412Interaction with PCNABy similarityAdd
BLAST
Regioni306 – 603298Interaction with the PRC2/EED-EZH2 complexBy similarityAdd
BLAST
Regioni329 – 548220DNA replication foci-targeting sequenceBy similarityAdd
BLAST
Regioni690 – 75162Autoinhibitory linkerAdd
BLAST
Regioni1106 – 1115105 X 2 AA tandem repeats of K-G
Regioni1118 – 1611494Interaction with the PRC2/EED-EZH2 complexBy similarityAdd
BLAST
Regioni1136 – 1611476CatalyticBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi177 – 20428Nuclear localization signalSequence AnalysisAdd
BLAST

Domaini

The N-terminal part is required for homodimerization and acts as a regulatory domain.
The CXXC-type zinc finger specifically binds to unmethylated CpG dinucleotides, positioning the autoinhibitory linker between the DNA and the active site, thus providing a mechanism to ensure that only hemimethylated CpG dinucleotides undergo methylation.By similarity

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 2 BAH domains.PROSITE-ProRule annotation
Contains 1 CXXC-type zinc finger.PROSITE-ProRule annotation
Contains 1 DMAP-interaction domain.Curated
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri643 – 68947CXXC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0270.
GeneTreeiENSGT00390000005100.
HOGENOMiHOG000082497.
HOVERGENiHBG051384.
InParanoidiQ24K09.
KOiK00558.
OMAiCPNLAVK.
OrthoDBiEOG77WWBH.
TreeFamiTF328926.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR010506. DMAP1-bd.
IPR017198. DNA_C5-MeTrfase_1_euk.
IPR029063. SAM-dependent_MTases-like.
IPR002857. Znf_CXXC.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF01426. BAH. 2 hits.
PF06464. DMAP_binding. 1 hit.
PF00145. DNA_methylase. 1 hit.
PF12047. DNMT1-RFD. 1 hit.
PF02008. zf-CXXC. 1 hit.
[Graphical view]
PIRSFiPIRSF037404. DNMT1. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
PS51058. ZF_CXXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24K09-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPARTAPARV PALASRAFSL PDDVRRRLKD LERDSLTEKE CVKEKLNLLH
60 70 80 90 100
EFLRTEIKNQ LCDLETKLHK EELSEEGYLA KVKSLLNKDL SLENGAHAFS
110 120 130 140 150
REANGCLENG SQTSGEDCRV VMAEKGKPPK PVSRLYTPRR SKSDGETKSE
160 170 180 190 200
VSSSPRITRK TTRQTTITSH FPRGPAKRKP EEEPEKVKSD DSVDEEKDQE
210 220 230 240 250
EKRRRVTSRE RVAGLLPAEE PGRVRPGTHM EEEGRDDKEE KRLRSQTKEP
260 270 280 290 300
TPKHKAKEEP DRDVRPGGAQ AEMNEGEDKD EKRHRSQPKD LASKRRPEEK
310 320 330 340 350
EPERVKPQVS DEKDEDEKEE KRRRTTYREL TEKKMTRTKI AVVSKTNPPK
360 370 380 390 400
CTECLQYLDD PELRYEQHPP DAVEEIQILT NERLSIFDAN ESGFESYEDL
410 420 430 440 450
PQHKLTCFSV YCKRGHLCPI DTGLIEKDVE LLFSGSAKPI YEDDPSPEGG
460 470 480 490 500
INGKNFGPIN EWWIAGFDGG EKALLGFSTS FAEYILMDPS PEYAPLFSVM
510 520 530 540 550
QEKIYISKIV VEFLQSNPDS TYEDLINKIE TTVPPCMLNL NRFTEDSLLR
560 570 580 590 600
HAQFVVEQVE SYDRAGDSDE QPIFLSPCMR DLIKLAGVTL GKRRAERRQT
610 620 630 640 650
IRQPAKEKDK GPTKATTTKL VYQIFDTFFA EQIEKDDKED KENAFKRRRC
660 670 680 690 700
GVCEICQQPE CGKCKACKDM VKFGGSGRSK QACQKRRCPN MAMKEADDDE
710 720 730 740 750
EVDDNIPEMP SPKKMHQGKK KKQNKNRISW VGDAVKTDGK KSYYKKVCID
760 770 780 790 800
SETLEVGDCV SVIPDDSSKP LYLARVTALW EDSSNGQMFH AHWFCAGTDT
810 820 830 840 850
VLGATSDPLE LFLVDECEDM QLSYIHSKVQ VIYKAPSENW AMEGGVDPEA
860 870 880 890 900
LMSEDDGKTY FYQLWYDQDY ARFESPPKTQ PTEDNKYKFC ASCARLAEMR
910 920 930 940 950
QKEIPRVVEQ LQDLEGRVLY SLATKNGVQY RVGDGVYLPP EAFTFNIKLS
960 970 980 990 1000
SPVKRPRKEP VDEALYPEHY RKYSDYIKGS NLDAPEPYRI GRIKEIFCSK
1010 1020 1030 1040 1050
KSNGRPNETD IKIRVNKFYR PENTHKSTPA SYHADINLLY WSDEEAVVDF
1060 1070 1080 1090 1100
KAVQGRCTVE YGEDLPQCLQ DFSAGGPDRF YFLEAYNAKS KSFEDPPNHA
1110 1120 1130 1140 1150
RSTGNKGKGK GKGKNRTKSQ TCEPSELETE IKLPKLRTLD VFSGCGGLSE
1160 1170 1180 1190 1200
GFHQAGISET LWAIEMWDPA AQAFRLNNPG STVFTEDCNV LLKLVMAGEV
1210 1220 1230 1240 1250
TNSRGQKLPQ KGDVEMLCGG PPCQGFSGMN RFNSRTYSKF KNSLVVSFLS
1260 1270 1280 1290 1300
YCDYYRPRYF LLENVRNFVS FKRSMVLKLT LRCLVRMGYQ CTFGVLQAGQ
1310 1320 1330 1340 1350
YGVAQTRRRA IILAAAPGEP LPLFPEPLHV FAPRACQLSV VVDDKKFVSN
1360 1370 1380 1390 1400
ITRLSSGPFR TITVRDTMSD LPEIRNGASA LEISYNGEPQ SWFQRQLRGS
1410 1420 1430 1440 1450
QYQPILRDHI CKDMSALVAA RMRHIPLAPG SDWRDLPNIE VRLSDGTLAR
1460 1470 1480 1490 1500
KLRYNYHDKK NGCSSSGALR GVCSCVEGKP CEPAARQFNT LIPWCLPHTG
1510 1520 1530 1540 1550
NRHNHWAGLY GRLEWDGFFS TTVTNPEPMG KQGRVLHPEQ HRVVSVRECA
1560 1570 1580 1590 1600
RSQGFPDTYR LFGNILDKHR QVGNAVPPPL AKAIGLEIKR CMLAKARESA
1610
SAKIKEEAAK D
Length:1,611
Mass (Da):182,842
Last modified:April 18, 2006 - v1
Checksum:iA7F0D9B24A18771C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti655 – 6551I → V in AAO44952. 1 PublicationCurated
Sequence conflicti716 – 7161H → R in AAO44952. 1 PublicationCurated
Sequence conflicti777 – 7771T → A in AAO44952. 1 PublicationCurated
Sequence conflicti1176 – 11761L → F in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1186 – 11861E → K in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1192 – 11921L → V in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1209 – 12091P → L in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1287 – 12871M → R in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1387 – 13871G → R in AAP20551. (PubMed:12971987)Curated
Sequence conflicti1405 – 14051I → T in AAO44952. 1 PublicationCurated
Sequence conflicti1466 – 14661S → T in AAO44952. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY244709 mRNA. Translation: AAP20551.1.
AY173048 mRNA. Translation: AAO44952.1.
BC114063 mRNA. Translation: AAI14064.1.
RefSeqiNP_872592.2. NM_182651.2.
UniGeneiBt.108052.
Bt.48560.

Genome annotation databases

EnsembliENSBTAT00000003549; ENSBTAP00000003549; ENSBTAG00000002736.
GeneIDi281119.
KEGGibta:281119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY244709 mRNA. Translation: AAP20551.1 .
AY173048 mRNA. Translation: AAO44952.1 .
BC114063 mRNA. Translation: AAI14064.1 .
RefSeqi NP_872592.2. NM_182651.2.
UniGenei Bt.108052.
Bt.48560.

3D structure databases

ProteinModelPortali Q24K09.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 158481. 1 interaction.

Protein family/group databases

REBASEi 7406. M.BtaDnmt1AP.

Proteomic databases

PRIDEi Q24K09.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000003549 ; ENSBTAP00000003549 ; ENSBTAG00000002736 .
GeneIDi 281119.
KEGGi bta:281119.

Organism-specific databases

CTDi 1786.

Phylogenomic databases

eggNOGi COG0270.
GeneTreei ENSGT00390000005100.
HOGENOMi HOG000082497.
HOVERGENi HBG051384.
InParanoidi Q24K09.
KOi K00558.
OMAi CPNLAVK.
OrthoDBi EOG77WWBH.
TreeFami TF328926.

Enzyme and pathway databases

Reactomei REACT_207393. PRC2 methylates histones and DNA.
REACT_214253. NoRC negatively regulates rRNA expression.

Miscellaneous databases

NextBioi 20805188.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR010506. DMAP1-bd.
IPR017198. DNA_C5-MeTrfase_1_euk.
IPR029063. SAM-dependent_MTases-like.
IPR002857. Znf_CXXC.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF01426. BAH. 2 hits.
PF06464. DMAP_binding. 1 hit.
PF00145. DNA_methylase. 1 hit.
PF12047. DNMT1-RFD. 1 hit.
PF02008. zf-CXXC. 1 hit.
[Graphical view ]
PIRSFi PIRSF037404. DNMT1. 1 hit.
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00439. BAH. 2 hits.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
PS51058. ZF_CXXC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and expression in bovine preimplantation embryos, fetal and adult tissues."
    Golding M.C., Westhusin M.E.
    Gene Expr. Patterns 3:551-558(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Bovine (Bos taurus) cytosine-5-methyltransferase (Dnmt1) cDNA."
    Lee P., Min K.S., Seong H.H., Park J.K., Lee Y.K., Kim S.W., Kim S.J., Lee H.G., Chung H.K., Chang Y.M., Chang W.K., Kwon M.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiDNMT1_BOVIN
AccessioniPrimary (citable) accession number: Q24K09
Secondary accession number(s): Q6Y856, Q7YS60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: April 18, 2006
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3