ID   IDE_BOVIN               Reviewed;        1019 AA.
AC   Q24K02;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Insulin-degrading enzyme;
DE            EC=3.4.24.56;
DE   AltName: Full=Insulin protease;
DE            Short=Insulinase;
DE   AltName: Full=Insulysin;
GN   Name=IDE;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a role in the cellular processing of insulin.
CC       May be involved in intercellular peptide signaling (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Degradation of insulin, glucagon and other
CC       polypeptides. No action on proteins.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Homodimer (Probable).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
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DR   EMBL; BC114105; AAI14106.1; -; mRNA.
DR   IPI; IPI00716156; -.
DR   RefSeq; NP_001069317.1; -.
DR   UniGene; Bt.6675; -.
DR   SMR; Q24K02; 45-1016.
DR   MEROPS; M16.002; -.
DR   MEROPS; M16.984; -.
DR   Ensembl; ENSBTAG00000019759; Bos taurus.
DR   GeneID; 523752; -.
DR   KEGG; bta:523752; -.
DR   HOVERGEN; Q24K02; -.
DR   BRENDA; 3.4.24.56; 251.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR011237; Pept_M16_core.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   Gene3D; G3DSA:3.30.830.10; Pept_M16_core; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Metal-binding; Metalloprotease; Protease; Zinc.
FT   CHAIN         1   1019       Insulin-degrading enzyme.
FT                                /FTId=PRO_0000283041.
FT   ACT_SITE    111    111       Proton acceptor (By similarity).
FT   METAL       108    108       Zinc (By similarity).
FT   METAL       112    112       Zinc (By similarity).
FT   METAL       189    189       Zinc (By similarity).
SQ   SEQUENCE   1019 AA;  118102 MW;  E3B589575B95C861 CRC64;
     MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI GHHIIKSHED
     KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP PNIAGLSHFC EHMLFLGTKK
     YPKENEYSQF LSEHAGSSNA FTSGEHTNYY FDVSHEHLEG ALDRFAQFFL CPLFDESCKD
     REVNAVDSEH EKNVMNDAWR LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE
     LLKFHSTYYS SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ
     LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL KSKGWVNTLV
     GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK LRAEGPQEWV FQECKDLNAV
     AFRFKDKERP RGYTSKIAGI LHYYPLEEVL TAEYLLEEFR PDLIEMVLDK LRPENVRVAI
     VSKSFEGKTD RTEEWYGTQY KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL
     EKEATPYPSL IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL
     KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA TFEIDEKRFE
     IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL KEALDDVTLP RLKAFIPQLL
     SRLHIEALLH GNITKQAALG IMQMVEDTLI EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ
     QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA
     NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL
     SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR RHKVSVHVLA
     REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR GLPLFPLVKP HINFMAAKL
//