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Q24K02

- IDE_BOVIN

UniProt

Q24K02 - IDE_BOVIN

Protein

Insulin-degrading enzyme

Gene

IDE

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP By similarity.By similarity

    Catalytic activityi

    Degradation of insulin, glucagon and other polypeptides. No action on proteins.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    Activated by ATP, other nucleotide triphosphates and small peptides. Inhibited by bacitracin By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081ZincPROSITE-ProRule annotation
    Active sitei111 – 1111Proton acceptorPROSITE-ProRule annotation
    Metal bindingi112 – 1121ZincPROSITE-ProRule annotation
    Metal bindingi189 – 1891ZincPROSITE-ProRule annotation
    Binding sitei429 – 4291ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi895 – 9017ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. insulin binding Source: Ensembl
    3. metalloendopeptidase activity Source: UniProtKB
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. beta-amyloid metabolic process Source: UniProtKB
    2. bradykinin catabolic process Source: UniProtKB
    3. determination of adult lifespan Source: Ensembl
    4. insulin catabolic process Source: Ensembl
    5. positive regulation of protein oligomerization Source: Ensembl
    6. protein homooligomerization Source: Ensembl
    7. proteolysis involved in cellular protein catabolic process Source: Ensembl
    8. ubiquitin homeostasis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Protein family/group databases

    MEROPSiM16.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-degrading enzyme (EC:3.4.24.56)
    Alternative name(s):
    Insulin protease
    Short name:
    Insulinase
    Insulysin
    Gene namesi
    Name:IDE
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 26

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity. Secreted By similarity

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. cytosol Source: Ensembl
    3. extracellular space Source: Ensembl
    4. mitochondrion Source: Ensembl
    5. nucleus Source: Ensembl
    6. peroxisome Source: Ensembl
    7. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10191019Insulin-degrading enzymePRO_0000283041Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921N6-succinyllysineBy similarity
    Modified residuei697 – 6971N6-succinyllysineBy similarity

    Proteomic databases

    PRIDEiQ24K02.

    Interactioni

    Subunit structurei

    Homodimer. Can form higher oligomers By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000026332.

    Structurei

    3D structure databases

    ProteinModelPortaliQ24K02.
    SMRiQ24K02. Positions 43-1016.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi853 – 8586SlyX motif

    Domaini

    The SlyX motif may be involved in the non-conventional secretion of the protein.By similarity

    Sequence similaritiesi

    Belongs to the peptidase M16 family.Curated

    Phylogenomic databases

    eggNOGiCOG1025.
    GeneTreeiENSGT00530000063327.
    HOGENOMiHOG000161331.
    HOVERGENiHBG106799.
    InParanoidiQ24K02.
    KOiK01408.
    OMAiSIFHIIK.
    OrthoDBiEOG7HHWRD.
    TreeFamiTF106275.

    Family and domain databases

    Gene3Di3.30.830.10. 4 hits.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view]
    PfamiPF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF63411. SSF63411. 4 hits.
    PROSITEiPS00143. INSULINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q24K02-1 [UniParc]FASTAAdd to Basket

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    MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI     50
    GHHIIKSHED KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP 100
    PNIAGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY 150
    FDVSHEHLEG ALDRFAQFFL CPLFDESCKD REVNAVDSEH EKNVMNDAWR 200
    LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE LLKFHSTYYS 250
    SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ 300
    LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL 350
    KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK 400
    LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGI LHYYPLEEVL 450
    TAEYLLEEFR PDLIEMVLDK LRPENVRVAI VSKSFEGKTD RTEEWYGTQY 500
    KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL EKEATPYPSL 550
    IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL 600
    KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA 650
    TFEIDEKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL 700
    KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG IMQMVEDTLI 750
    EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ 800
    STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII 850
    QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL 900
    SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR 950
    RHKVSVHVLA REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR 1000
    GLPLFPLVKP HINFMAAKL 1019
    Length:1,019
    Mass (Da):118,102
    Last modified:April 18, 2006 - v1
    Checksum:iE3B589575B95C861
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114105 mRNA. Translation: AAI14106.1.
    RefSeqiNP_001069317.1. NM_001075849.1.
    UniGeneiBt.6675.

    Genome annotation databases

    EnsembliENSBTAT00000026332; ENSBTAP00000026332; ENSBTAG00000019759.
    GeneIDi523752.
    KEGGibta:523752.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114105 mRNA. Translation: AAI14106.1 .
    RefSeqi NP_001069317.1. NM_001075849.1.
    UniGenei Bt.6675.

    3D structure databases

    ProteinModelPortali Q24K02.
    SMRi Q24K02. Positions 43-1016.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000026332.

    Protein family/group databases

    MEROPSi M16.002.

    Proteomic databases

    PRIDEi Q24K02.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000026332 ; ENSBTAP00000026332 ; ENSBTAG00000019759 .
    GeneIDi 523752.
    KEGGi bta:523752.

    Organism-specific databases

    CTDi 3416.

    Phylogenomic databases

    eggNOGi COG1025.
    GeneTreei ENSGT00530000063327.
    HOGENOMi HOG000161331.
    HOVERGENi HBG106799.
    InParanoidi Q24K02.
    KOi K01408.
    OMAi SIFHIIK.
    OrthoDBi EOG7HHWRD.
    TreeFami TF106275.

    Miscellaneous databases

    NextBioi 20873816.

    Family and domain databases

    Gene3Di 3.30.830.10. 4 hits.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view ]
    Pfami PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF63411. SSF63411. 4 hits.
    PROSITEi PS00143. INSULINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.

    Entry informationi

    Entry nameiIDE_BOVIN
    AccessioniPrimary (citable) accession number: Q24K02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ATP-binding induces a conformation change.By similarity

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3