Q24K02 (IDE_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-degrading enzyme
EC=3.4.24.56

Alternative name(s):
Insulin protease
Short name=Insulinase
Insulysin

Gene names

Name:

IDE

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1019 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP By similarity.
Catalytic activity	Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Activated by ATP, other nucleotide triphosphates and small peptides. Inhibited by bacitracin By similarity.
Subunit structure	Homodimer. Can form higher oligomers By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Secreted By similarity.
Domain	The SlyX motif may be involved in the non-conventional secretion of the protein By similarity.
Miscellaneous	ATP-binding induces a conformation change By similarity.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Secreted
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	beta-amyloid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB bradykinin catabolic process Inferred from sequence or structural similarity. Source: UniProtKB determination of adult lifespan Inferred from electronic annotation. Source: Compara insulin catabolic process Inferred from electronic annotation. Source: Compara positive regulation of protein oligomerization Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara proteolysis involved in cellular protein catabolic process Inferred from electronic annotation. Source: Compara ubiquitin homeostasis Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara cytosol Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara peroxisome Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB insulin binding Inferred from electronic annotation. Source: Compara metalloendopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1019	1019	Insulin-degrading enzyme		PRO_0000283041
Regions
	Nucleotide binding	895 – 901	7	ATP By similarity
	Motif	853 – 858	6	SlyX motif
Sites
	Active site	111	1	Proton acceptor By similarity
	Metal binding	108	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	189	1	Zinc By similarity
	Binding site	429	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q24K02 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: E3B589575B95C861	FASTA	1,019	118,102
	10 20 30 40 50 60 MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI GHHIIKSHED 70 80 90 100 110 120 KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP PNIAGLSHFC EHMLFLGTKK 130 140 150 160 170 180 YPKENEYSQF LSEHAGSSNA FTSGEHTNYY FDVSHEHLEG ALDRFAQFFL CPLFDESCKD 190 200 210 220 230 240 REVNAVDSEH EKNVMNDAWR LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE 250 260 270 280 290 300 LLKFHSTYYS SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ 310 320 330 340 350 360 LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL KSKGWVNTLV 370 380 390 400 410 420 GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK LRAEGPQEWV FQECKDLNAV 430 440 450 460 470 480 AFRFKDKERP RGYTSKIAGI LHYYPLEEVL TAEYLLEEFR PDLIEMVLDK LRPENVRVAI 490 500 510 520 530 540 VSKSFEGKTD RTEEWYGTQY KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL 550 560 570 580 590 600 EKEATPYPSL IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL 610 620 630 640 650 660 KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA TFEIDEKRFE 670 680 690 700 710 720 IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL KEALDDVTLP RLKAFIPQLL 730 740 750 760 770 780 SRLHIEALLH GNITKQAALG IMQMVEDTLI EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ 790 800 810 820 830 840 QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA 850 860 870 880 890 900 NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL 910 920 930 940 950 960 SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR RHKVSVHVLA 970 980 990 1000 1010 REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR GLPLFPLVKP HINFMAAKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC114105 mRNA. Translation: AAI14106.1.
IPI	IPI00716156.
RefSeq	NP_001069317.1. NM_001075849.1.
UniGene	Bt.6675.
3D structure databases
ProteinModelPortal	Q24K02.
SMR	Q24K02. Positions 43-1016.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000026332.
Protein family/group databases
MEROPS	M16.984.
Proteomic databases
PRIDE	Q24K02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000026332; ENSBTAP00000026332; ENSBTAG00000019759.
GeneID	523752.
KEGG	bta:523752.
Organism-specific databases
CTD	3416.
Phylogenomic databases
eggNOG	COG1025.
GeneTree	ENSGT00530000063327.
HOGENOM	HOG000161331.
HOVERGEN	HBG106799.
InParanoid	Q24K02.
KO	K01408.
OMA	FFHELRT.
OrthoDB	EOG4H4630.
Family and domain databases
Gene3D	3.30.830.10. 4 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 2 hits. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 4 hits.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20873816.

Entry information

Entry name

IDE_BOVIN

Accession

Primary (citable) accession number: Q24K02

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program