Reviewed, UniProtKB/Swiss-Prot Q24K02 (IDE_BOVIN)

Names and origin

Protein names

Recommended name:
    Insulin-degrading enzyme
    EC=3.4.24.56
Alternative name(s):
    Insulin protease
      Short name=Insulinase
    Insulysin

Gene names

Name:

IDE

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1019 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the cellular processing of insulin. May be involved in intercellular peptide signaling By similarity.
Catalytic activity	Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer Probable.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1019	1019	Insulin-degrading enzyme		PRO_0000283041
Sites
	Active site	111	1	Proton acceptor By similarity
	Metal binding	108	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	189	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q24K02-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: E3B589575B95C861	FASTA	1,019	118,102
	10 20 30 40 50 60 MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI GHHIIKSHED 70 80 90 100 110 120 KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP PNIAGLSHFC EHMLFLGTKK 130 140 150 160 170 180 YPKENEYSQF LSEHAGSSNA FTSGEHTNYY FDVSHEHLEG ALDRFAQFFL CPLFDESCKD 190 200 210 220 230 240 REVNAVDSEH EKNVMNDAWR LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE 250 260 270 280 290 300 LLKFHSTYYS SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ 310 320 330 340 350 360 LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL KSKGWVNTLV 370 380 390 400 410 420 GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK LRAEGPQEWV FQECKDLNAV 430 440 450 460 470 480 AFRFKDKERP RGYTSKIAGI LHYYPLEEVL TAEYLLEEFR PDLIEMVLDK LRPENVRVAI 490 500 510 520 530 540 VSKSFEGKTD RTEEWYGTQY KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL 550 560 570 580 590 600 EKEATPYPSL IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL 610 620 630 640 650 660 KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA TFEIDEKRFE 670 680 690 700 710 720 IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL KEALDDVTLP RLKAFIPQLL 730 740 750 760 770 780 SRLHIEALLH GNITKQAALG IMQMVEDTLI EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ 790 800 810 820 830 840 QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA 850 860 870 880 890 900 NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL 910 920 930 940 950 960 SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR RHKVSVHVLA 970 980 990 1000 1010 REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR GLPLFPLVKP HINFMAAKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC114105 mRNA. Translation: AAI14106.1.
IPI	IPI00716156.
RefSeq	NP_001069317.1.
UniGene	Bt.6675
3D structure databases
SMR	Q24K02. Positions 45-1016.
ModBase	Search...
Protein family/group databases
MEROPS	M16.002. M16.984.
Genome annotation databases
Ensembl	ENSBTAG00000019759. Bos taurus. [Contig view]
GeneID	523752.
KEGG	bta:523752.
Phylogenomic databases
HOVERGEN	Q24K02.
Enzyme and pathway databases
BRENDA	3.4.24.56. 251.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 1 hit.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 2 hits. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDE_BOVIN

Accession

Primary (citable) accession number: Q24K02

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)