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Q24K02 (IDE_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-degrading enzyme

EC=3.4.24.56
Alternative name(s):
Insulin protease
Short name=Insulinase
Insulysin
Gene names
Name:IDE
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1019 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP By similarity.

Catalytic activity

Degradation of insulin, glucagon and other polypeptides. No action on proteins.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Activated by ATP, other nucleotide triphosphates and small peptides. Inhibited by bacitracin By similarity.

Subunit structure

Homodimer. Can form higher oligomers By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity. Secreted By similarity.

Domain

The SlyX motif may be involved in the non-conventional secretion of the protein By similarity.

Miscellaneous

ATP-binding induces a conformation change By similarity.

Sequence similarities

Belongs to the peptidase M16 family.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
Secreted
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbeta-amyloid metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

bradykinin catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

determination of adult lifespan

Inferred from electronic annotation. Source: Ensembl

insulin catabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein oligomerization

Inferred from electronic annotation. Source: Ensembl

protein homooligomerization

Inferred from electronic annotation. Source: Ensembl

proteolysis involved in cellular protein catabolic process

Inferred from electronic annotation. Source: Ensembl

ubiquitin homeostasis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

peroxisome

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

insulin binding

Inferred from electronic annotation. Source: Ensembl

metalloendopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10191019Insulin-degrading enzyme
PRO_0000283041

Regions

Nucleotide binding895 – 9017ATP By similarity
Motif853 – 8586SlyX motif

Sites

Active site1111Proton acceptor By similarity
Metal binding1081Zinc By similarity
Metal binding1121Zinc By similarity
Metal binding1891Zinc By similarity
Binding site4291ATP By similarity

Amino acid modifications

Modified residue1921N6-succinyllysine By similarity
Modified residue6971N6-succinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q24K02 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: E3B589575B95C861

FASTA1,019118,102
        10         20         30         40         50         60 
MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI GHHIIKSHED 

        70         80         90        100        110        120 
KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP PNIAGLSHFC EHMLFLGTKK 

       130        140        150        160        170        180 
YPKENEYSQF LSEHAGSSNA FTSGEHTNYY FDVSHEHLEG ALDRFAQFFL CPLFDESCKD 

       190        200        210        220        230        240 
REVNAVDSEH EKNVMNDAWR LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE 

       250        260        270        280        290        300 
LLKFHSTYYS SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ 

       310        320        330        340        350        360 
LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL KSKGWVNTLV 

       370        380        390        400        410        420 
GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK LRAEGPQEWV FQECKDLNAV 

       430        440        450        460        470        480 
AFRFKDKERP RGYTSKIAGI LHYYPLEEVL TAEYLLEEFR PDLIEMVLDK LRPENVRVAI 

       490        500        510        520        530        540 
VSKSFEGKTD RTEEWYGTQY KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL 

       550        560        570        580        590        600 
EKEATPYPSL IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL 

       610        620        630        640        650        660 
KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA TFEIDEKRFE 

       670        680        690        700        710        720 
IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL KEALDDVTLP RLKAFIPQLL 

       730        740        750        760        770        780 
SRLHIEALLH GNITKQAALG IMQMVEDTLI EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ 

       790        800        810        820        830        840 
QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA 

       850        860        870        880        890        900 
NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL 

       910        920        930        940        950        960 
SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR RHKVSVHVLA 

       970        980        990       1000       1010 
REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR GLPLFPLVKP HINFMAAKL 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Hypothalamus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC114105 mRNA. Translation: AAI14106.1.
RefSeqNP_001069317.1. NM_001075849.1.
UniGeneBt.6675.

3D structure databases

ProteinModelPortalQ24K02.
SMRQ24K02. Positions 43-1016.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000026332.

Protein family/group databases

MEROPSM16.002.

Proteomic databases

PRIDEQ24K02.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000026332; ENSBTAP00000026332; ENSBTAG00000019759.
GeneID523752.
KEGGbta:523752.

Organism-specific databases

CTD3416.

Phylogenomic databases

eggNOGCOG1025.
GeneTreeENSGT00530000063327.
HOGENOMHOG000161331.
HOVERGENHBG106799.
InParanoidQ24K02.
KOK01408.
OMASIFHIIK.
OrthoDBEOG7HHWRD.
TreeFamTF106275.

Family and domain databases

Gene3D3.30.830.10. 4 hits.
InterProIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 2 hits.
[Graphical view]
SUPFAMSSF63411. SSF63411. 4 hits.
PROSITEPS00143. INSULINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20873816.

Entry information

Entry nameIDE_BOVIN
AccessionPrimary (citable) accession number: Q24K02
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries