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Protein

Insulin-degrading enzyme

Gene

IDE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP (By similarity).By similarity

Miscellaneous

ATP-binding induces a conformation change.By similarity

Catalytic activityi

Degradation of insulin, glucagon and other polypeptides. No action on proteins.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Activated by ATP, other nucleotide triphosphates and small peptides. Inhibited by bacitracin (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108ZincPROSITE-ProRule annotation1
Active sitei111Proton acceptorPROSITE-ProRule annotation1
Metal bindingi112ZincPROSITE-ProRule annotation1
Metal bindingi189ZincPROSITE-ProRule annotation1
Binding sitei429ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi895 – 901ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Protein family/group databases

MEROPSiM16.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-degrading enzyme (EC:3.4.24.56)
Alternative name(s):
Insulin protease
Short name:
Insulinase
Insulysin
Gene namesi
Name:IDE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002830411 – 1019Insulin-degrading enzymeAdd BLAST1019

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192N6-succinyllysineBy similarity1
Modified residuei697N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiQ24K02.
PRIDEiQ24K02.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019759.

Interactioni

Subunit structurei

Homodimer. Can form higher oligomers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026332.

Structurei

3D structure databases

ProteinModelPortaliQ24K02.
SMRiQ24K02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi853 – 858SlyX motif6

Domaini

The SlyX motif may be involved in the non-conventional secretion of the protein.By similarity

Sequence similaritiesi

Belongs to the peptidase M16 family.Curated

Phylogenomic databases

eggNOGiKOG0959. Eukaryota.
COG1025. LUCA.
GeneTreeiENSGT00530000063327.
HOGENOMiHOG000161331.
HOVERGENiHBG106799.
InParanoidiQ24K02.
KOiK01408.
OMAiMAHAVEH.
OrthoDBiEOG091G0255.
TreeFamiTF106275.

Family and domain databases

InterProiView protein in InterPro
IPR011249. Metalloenz_LuxS/M16.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
IPR032632. Peptidase_M16_M.
PfamiView protein in Pfam
PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 2 hits.
PF16187. Peptidase_M16_M. 1 hit.
SUPFAMiSSF63411. SSF63411. 4 hits.
PROSITEiView protein in PROSITE
PS00143. INSULINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q24K02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYRLAWLLH SALPSTFRSV LGARLPPSER LCGFQKKTYS KMNNPAIKRI
60 70 80 90 100
GHHIIKSHED KREYRGLELA NGIKVLLVSD PTTDKSSAAL DVHIGSLSDP
110 120 130 140 150
PNIAGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY
160 170 180 190 200
FDVSHEHLEG ALDRFAQFFL CPLFDESCKD REVNAVDSEH EKNVMNDAWR
210 220 230 240 250
LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE LLKFHSTYYS
260 270 280 290 300
SNLMAICVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ
310 320 330 340 350
LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL
360 370 380 390 400
KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK
410 420 430 440 450
LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGI LHYYPLEEVL
460 470 480 490 500
TAEYLLEEFR PDLIEMVLDK LRPENVRVAI VSKSFEGKTD RTEEWYGTQY
510 520 530 540 550
KQEAIPDEVI KKWQNADLNG KFKLPMKNEF IPTNFEILSL EKEATPYPSL
560 570 580 590 600
IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL
610 620 630 640 650
KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA
660 670 680 690 700
TFEIDEKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL
710 720 730 740 750
KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG IMQMVEDTLI
760 770 780 790 800
EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ
810 820 830 840 850
STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII
860 870 880 890 900
QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL
910 920 930 940 950
SAECAKYWGE IISQQYNFDR DNIEVAYLKT LTKEDIIKFY KEMLAVDAPR
960 970 980 990 1000
RHKVSVHVLA REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR
1010
GLPLFPLVKP HINFMAAKL
Length:1,019
Mass (Da):118,102
Last modified:April 18, 2006 - v1
Checksum:iE3B589575B95C861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114105 mRNA. Translation: AAI14106.1.
RefSeqiNP_001069317.1. NM_001075849.1.
UniGeneiBt.6675.

Genome annotation databases

EnsembliENSBTAT00000026332; ENSBTAP00000026332; ENSBTAG00000019759.
GeneIDi523752.
KEGGibta:523752.

Similar proteinsi

Entry informationi

Entry nameiIDE_BOVIN
AccessioniPrimary (citable) accession number: Q24K02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 18, 2006
Last modified: August 30, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families