Q24JP5 (T132A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protein 132A

Alternative name(s):
HSPA5-binding protein 1

Gene names

Name:	TMEM132A
Synonyms:	HSPA5BP1, KIAA1583

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1023 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in embryonic and postnatal development of the brain. Increased resistance to cell death induced by serum starvation in cultured cells. Regulates cAMP-induced GFAP gene expression via STAT3 phosphorylation By similarity.
Subunit structure	Interacts with HSPA5/GRP78 By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the TMEM132 family.
Sequence caution	The sequence BAB13409.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14613.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q24JP5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q24JP5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     337-337: D → DS

Note: No experimental confirmation available.

Isoform 3 (identifier: Q24JP5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: MCARMAGRTTAAPRGPYGPWLCLLVALALDVVR → MAPGSAPASSGAVIISPSYASS
     405-423: AEELVNTAPLTGVPQHVPV → VRRPPSLPGKAGGQVPGAP
     424-1023: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q24JP5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     46-295: Missing.
     337-337: D → DS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 1023	988	Transmembrane protein 132A		PRO_0000287096
Regions
	Topological domain	36 – 852	817	Extracellular Potential
	Transmembrane	853 – 873	21	Helical; Potential
	Topological domain	874 – 1023	150	Cytoplasmic Potential
	Region	611 – 916	306	Binds to HSPA5/GRP78 By similarity
	Region	671 – 1023	353	Confers cellular localization similar to full-length form By similarity
	Compositional bias	812 – 835	24	Glu-rich
Amino acid modifications
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 33	33	MCARM…LDVVR → MAPGSAPASSGAVIISPSYA SS in isoform 3.		VSP_025302
	Alternative sequence	46 – 295	250	Missing in isoform 4.		VSP_025303
	Alternative sequence	337	1	D → DS in isoform 2 and isoform 4.		VSP_025304
	Alternative sequence	405 – 423	19	AEELV…QHVPV → VRRPPSLPGKAGGQVPGAP in isoform 3.		VSP_025305
	Alternative sequence	424 – 1023	600	Missing in isoform 3.		VSP_025306
	Natural variant	699	1	R → H. Corresponds to variant rs524523 [ dbSNP | Ensembl ].		VAR_032262
	Natural variant	969	1	A → V. Ref.2 Corresponds to variant rs2469887 [ dbSNP | Ensembl ].		VAR_032263
Experimental info
	Sequence conflict	10	1	T → R in BAA91245. Ref.2
	Sequence conflict	824	1	E → K in BAB14613. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 464408B227D764C8	FASTA	1,023	110,110
	10 20 30 40 50 60 MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLPAAL ELLDAPEHFR 70 80 90 100 110 120 VQQVGHYPPA NSSLSSRSET FLLLQPWPRA QPLLRASYPP FATQQVVPPR VTEPHQRPVP 130 140 150 160 170 180 WDVRAVSVEA AVTPAEPYAR VLFHLKGQDW PPGSGSLPCA RLHATHPAGT AHQACRFQPS 190 200 210 220 230 240 LGACVVELEL PSHWFSQAST TRAELAYTLE PAAEGPGGCG SGEENDPGEQ ALPVGGVELR 250 260 270 280 290 300 PADPPQYQEV PLDEAVTLRV PDMPVRPGQL FSATLLLRHN FTASLLTLRI KVKKGLHVTA 310 320 330 340 350 360 ARPAQPTLWT AKLDRFKGSR HHTTLITCHR AGLTEPDSSP LELSEFLWVD FVVENSTGGG 370 380 390 400 410 420 VAVTRPVTWQ LEYPGQAPEA EKDKMVWEIL VSERDIRALI PLAKAEELVN TAPLTGVPQH 430 440 450 460 470 480 VPVRLVTVDG GGALVEVTEH VGCESANTQV LQVSEACDAV FVAGKESRGA RGVRVDFWWR 490 500 510 520 530 540 RLRASLRLTV WAPLLPLRIE LTDTTLEQVR GWRVPGPAEG PAEPAAEASD EAERRARGCH 550 560 570 580 590 600 LQYQRAGVRF LAPFAAHPLD GGRRLTHLLG PDWLLDVSHL VAPHARVLDS RVASLEGGRV 610 620 630 640 650 660 VVGREPGVTS IEVRSPLSDS ILGEQALAVT DDKVSVLELR VQPVMGISLT LSRGTAHPGE 670 680 690 700 710 720 VTATCWAQSA LPAPKQEVAL SLWLSFSDHT VAPAELYDRR DLGLSVSAEE PGAILPAEEQ 730 740 750 760 770 780 GAQLGVVVSG AGAEGLPLHV ALHPPEPCRR GRHRVPLASG TAWLGLPPAS TPAPALPSSP 790 800 810 820 830 840 AWSPPATEAT MGGKRQVAGS VGGNTGVRGK FERAEEEARK EETEAREEEE EEEEEMVPAP 850 860 870 880 890 900 QHVTELELGM YALLGVFCVA IFIFLVNGVV FVLRYQRKEP PDSATDPTSP QPHNWVWLGT 910 920 930 940 950 960 DQEELSRQLD RQSPGPPKGE GSCPCESGGG GEAPTLAPGP PGGTTSSSST LARKEAGGRR 970 980 990 1000 1010 1020 KRVEFVTFAP APPAQSPEEP VGAPAVQSIL VAGEEDIRWV CEDMGLKDPE ELRNYMERIR GSS
	Isoform 2 [UniParc]. Checksum: 0CF1381EF2F126CE	FASTA	1,024	110,197
	10 20 30 40 50 60 MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLPAAL ELLDAPEHFR 70 80 90 100 110 120 VQQVGHYPPA NSSLSSRSET FLLLQPWPRA QPLLRASYPP FATQQVVPPR VTEPHQRPVP 130 140 150 160 170 180 WDVRAVSVEA AVTPAEPYAR VLFHLKGQDW PPGSGSLPCA RLHATHPAGT AHQACRFQPS 190 200 210 220 230 240 LGACVVELEL PSHWFSQAST TRAELAYTLE PAAEGPGGCG SGEENDPGEQ ALPVGGVELR 250 260 270 280 290 300 PADPPQYQEV PLDEAVTLRV PDMPVRPGQL FSATLLLRHN FTASLLTLRI KVKKGLHVTA 310 320 330 340 350 360 ARPAQPTLWT AKLDRFKGSR HHTTLITCHR AGLTEPDSSS PLELSEFLWV DFVVENSTGG 370 380 390 400 410 420 GVAVTRPVTW QLEYPGQAPE AEKDKMVWEI LVSERDIRAL IPLAKAEELV NTAPLTGVPQ 430 440 450 460 470 480 HVPVRLVTVD GGGALVEVTE HVGCESANTQ VLQVSEACDA VFVAGKESRG ARGVRVDFWW 490 500 510 520 530 540 RRLRASLRLT VWAPLLPLRI ELTDTTLEQV RGWRVPGPAE GPAEPAAEAS DEAERRARGC 550 560 570 580 590 600 HLQYQRAGVR FLAPFAAHPL DGGRRLTHLL GPDWLLDVSH LVAPHARVLD SRVASLEGGR 610 620 630 640 650 660 VVVGREPGVT SIEVRSPLSD SILGEQALAV TDDKVSVLEL RVQPVMGISL TLSRGTAHPG 670 680 690 700 710 720 EVTATCWAQS ALPAPKQEVA LSLWLSFSDH TVAPAELYDR RDLGLSVSAE EPGAILPAEE 730 740 750 760 770 780 QGAQLGVVVS GAGAEGLPLH VALHPPEPCR RGRHRVPLAS GTAWLGLPPA STPAPALPSS 790 800 810 820 830 840 PAWSPPATEA TMGGKRQVAG SVGGNTGVRG KFERAEEEAR KEETEAREEE EEEEEEMVPA 850 860 870 880 890 900 PQHVTELELG MYALLGVFCV AIFIFLVNGV VFVLRYQRKE PPDSATDPTS PQPHNWVWLG 910 920 930 940 950 960 TDQEELSRQL DRQSPGPPKG EGSCPCESGG GGEAPTLAPG PPGGTTSSSS TLARKEAGGR 970 980 990 1000 1010 1020 RKRVEFVTFA PAPPAQSPEE PVGAPAVQSI LVAGEEDIRW VCEDMGLKDP EELRNYMERI RGSS
	Isoform 3 [UniParc]. Checksum: D64FED452F29A03C	FASTA	412	44,358
	10 20 30 40 50 60 MAPGSAPASS GAVIISPSYA SSVDCGQAPL DPVYLPAALE LLDAPEHFRV QQVGHYPPAN 70 80 90 100 110 120 SSLSSRSETF LLLQPWPRAQ PLLRASYPPF ATQQVVPPRV TEPHQRPVPW DVRAVSVEAA 130 140 150 160 170 180 VTPAEPYARV LFHLKGQDWP PGSGSLPCAR LHATHPAGTA HQACRFQPSL GACVVELELP 190 200 210 220 230 240 SHWFSQASTT RAELAYTLEP AAEGPGGCGS GEENDPGEQA LPVGGVELRP ADPPQYQEVP 250 260 270 280 290 300 LDEAVTLRVP DMPVRPGQLF SATLLLRHNF TASLLTLRIK VKKGLHVTAA RPAQPTLWTA 310 320 330 340 350 360 KLDRFKGSRH HTTLITCHRA GLTEPDSSPL ELSEFLWVDF VVENSTGGGV AVTRPVTWQL 370 380 390 400 410 EYPGQAPEAE KDKMVWEILV SERDIRALIP LAKVRRPPSL PGKAGGQVPG AP
	Isoform 4 [UniParc]. Checksum: 7CE22843F97A190E	FASTA	774	83,098
	10 20 30 40 50 60 MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLHVTA ARPAQPTLWT 70 80 90 100 110 120 AKLDRFKGSR HHTTLITCHR AGLTEPDSSS PLELSEFLWV DFVVENSTGG GVAVTRPVTW 130 140 150 160 170 180 QLEYPGQAPE AEKDKMVWEI LVSERDIRAL IPLAKAEELV NTAPLTGVPQ HVPVRLVTVD 190 200 210 220 230 240 GGGALVEVTE HVGCESANTQ VLQVSEACDA VFVAGKESRG ARGVRVDFWW RRLRASLRLT 250 260 270 280 290 300 VWAPLLPLRI ELTDTTLEQV RGWRVPGPAE GPAEPAAEAS DEAERRARGC HLQYQRAGVR 310 320 330 340 350 360 FLAPFAAHPL DGGRRLTHLL GPDWLLDVSH LVAPHARVLD SRVASLEGGR VVVGREPGVT 370 380 390 400 410 420 SIEVRSPLSD SILGEQALAV TDDKVSVLEL RVQPVMGISL TLSRGTAHPG EVTATCWAQS 430 440 450 460 470 480 ALPAPKQEVA LSLWLSFSDH TVAPAELYDR RDLGLSVSAE EPGAILPAEE QGAQLGVVVS 490 500 510 520 530 540 GAGAEGLPLH VALHPPEPCR RGRHRVPLAS GTAWLGLPPA STPAPALPSS PAWSPPATEA 550 560 570 580 590 600 TMGGKRQVAG SVGGNTGVRG KFERAEEEAR KEETEAREEE EEEEEEMVPA PQHVTELELG 610 620 630 640 650 660 MYALLGVFCV AIFIFLVNGV VFVLRYQRKE PPDSATDPTS PQPHNWVWLG TDQEELSRQL 670 680 690 700 710 720 DRQSPGPPKG EGSCPCESGG GGEAPTLAPG PPGGTTSSSS TLARKEAGGR RKRVEFVTFA 730 740 750 760 770 PAPPAQSPEE PVGAPAVQSI LVAGEEDIRW VCEDMGLKDP EELRNYMERI RGSS

References

[1]	"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 364-1023 (ISOFORM 1), VARIANT VAL-969. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-560 (ISOFORM 2). Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 188-560 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046803 mRNA. Translation: BAB13409.1. Different initiation. AK000546 mRNA. Translation: BAA91245.1. AK023577 mRNA. Translation: BAB14613.1. Different initiation. BC028106 mRNA. Translation: AAH28106.1. BC032059 mRNA. Translation: AAH32059.1. BC114569 mRNA. Translation: AAI14570.1. AL359557 mRNA. Translation: CAH10668.2.
IPI	IPI00301865. IPI00383814. IPI00845230. IPI00845309.
RefSeq	NP_060340.2. NM_017870.3. NP_821174.1. NM_178031.2.
UniGene	Hs.118552.
3D structure databases
ProteinModelPortal	Q24JP5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q24JP5. 1 interaction.
STRING	9606.ENSP00000005286.
PTM databases
PhosphoSite	Q24JP5.
Polymorphism databases
DMDM	121940967.
Proteomic databases
PaxDb	Q24JP5.
PRIDE	Q24JP5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000005286; ENSP00000005286; ENSG00000006118. ENST00000453848; ENSP00000405823; ENSG00000006118.
GeneID	54972.
KEGG	hsa:54972.
UCSC	uc001nqi.3. human. uc001nqj.3. human. uc001nqk.3. human.
Organism-specific databases
CTD	54972.
GeneCards	GC11P060691.
HGNC	HGNC:31092. TMEM132A.
HPA	HPA051979.
neXtProt	NX_Q24JP5.
PharmGKB	PA134886953.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG253956.
HOVERGEN	HBG099719.
InParanoid	Q24JP5.
OMA	GACVVEL.
Gene expression databases
ArrayExpress	Q24JP5.
Bgee	Q24JP5.
CleanEx	HS_TMEM132A.
Genevestigator	Q24JP5.
Family and domain databases
InterPro	IPR026307. TMEM132. [Graphical view]
PANTHER	PTHR13388. PTHR13388. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	54972.
NextBio	58216.

Entry information

Entry name

T132A_HUMAN

Accession

Primary (citable) accession number: Q24JP5
Secondary accession number(s): Q69YU7 , Q86VZ8, Q86W97, Q9H8K3, Q9HCI9, Q9NWY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.