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Q24JJ8

- GLO3_ARATH

UniProt

Q24JJ8 - GLO3_ARATH

Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO3

Gene

GLO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071FMNPROSITE-ProRule annotation
    Binding sitei128 – 1281FMNPROSITE-ProRule annotation
    Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
    Binding sitei156 – 1561FMNPROSITE-ProRule annotation
    Binding sitei165 – 1651SubstratePROSITE-ProRule annotation
    Binding sitei228 – 2281FMNPROSITE-ProRule annotation
    Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation
    Binding sitei255 – 2551SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 30725FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    3. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    4. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response signaling pathway, resistance gene-independent Source: TAIR
    2. defense response to bacterium Source: TAIR
    3. hydrogen peroxide biosynthetic process Source: TAIR
    4. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolate pathway, Photorespiration

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciARA:AT3G14150-MONOMER.
    ARA:GQT-2460-MONOMER.
    UniPathwayiUPA00951; UER00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (EC:1.1.3.15)
    Alternative name(s):
    Glycolate oxidase 3
    Short name:
    AtGLO3
    Short name:
    GOX 3
    Short chain alpha-hydroxy acid oxidase GLO3
    Gene namesi
    Name:GLO3
    Ordered Locus Names:At3g14150
    ORF Names:MAG2.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G14150.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. peroxisome Source: TAIR

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363Peroxisomal (S)-2-hydroxy-acid oxidase GLO3PRO_0000403406Add
    BLAST

    Proteomic databases

    PaxDbiQ24JJ8.
    PRIDEiQ24JJ8.

    Expressioni

    Gene expression databases

    GenevestigatoriQ24JJ8.

    Interactioni

    Subunit structurei

    Homotetramer or homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT3G14150.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ24JJ8.
    SMRiQ24JJ8. Positions 3-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 357357FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi361 – 3633Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    HOGENOMiHOG000217463.
    InParanoidiQ24JJ8.
    KOiK11517.
    OMAiYVQHAEA.
    PhylomeDBiQ24JJ8.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q24JJ8-1 [UniParc]FASTAAdd to Basket

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    MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP    50
    RVLVDVSKID MSTKILGYPI SAPIMIAPTG NHKLAHPEGE TATAKAAAAC 100
    NTIMIVSYMS SCTFEEIASS CNAVRFLQIY VYKRRDITAQ VVKRAEKAGF 150
    KAIVLTVDVP RLGRREADIK NKMISPQLKN FEGLFSTEVR PSKGSGVQAF 200
    ASRAFDASFS WKDIEWLRSI TELPILVKGI LTREDALKAV EAGVDGIIVS 250
    NHGGRQLDYS PATITVLEEV VQVVRGRIPV LLDGGVRRGT DVFKALALGA 300
    QAVLIGRPII YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDITRN 350
    HVRTENERLH SML 363
    Length:363
    Mass (Da):40,130
    Last modified:April 18, 2006 - v1
    Checksum:i8AA94B1A354BA60C
    GO

    Sequence cautioni

    The sequence BAB02979.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 871P → L in AAM67194. 1 PublicationCurated
    Sequence conflicti309 – 3091I → M in AAM67194. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000600 Genomic DNA. Translation: BAB02979.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75477.1.
    CP002686 Genomic DNA. Translation: AEE75478.1.
    BT024891 mRNA. Translation: ABD85162.1.
    AY088888 mRNA. Translation: AAM67194.1.
    RefSeqiNP_001078152.1. NM_001084683.1.
    NP_188031.1. NM_112271.3.
    UniGeneiAt.39282.

    Genome annotation databases

    EnsemblPlantsiAT3G14150.1; AT3G14150.1; AT3G14150.
    AT3G14150.2; AT3G14150.2; AT3G14150.
    GeneIDi820632.
    KEGGiath:AT3G14150.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000600 Genomic DNA. Translation: BAB02979.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75477.1 .
    CP002686 Genomic DNA. Translation: AEE75478.1 .
    BT024891 mRNA. Translation: ABD85162.1 .
    AY088888 mRNA. Translation: AAM67194.1 .
    RefSeqi NP_001078152.1. NM_001084683.1.
    NP_188031.1. NM_112271.3.
    UniGenei At.39282.

    3D structure databases

    ProteinModelPortali Q24JJ8.
    SMRi Q24JJ8. Positions 3-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G14150.1-P.

    Proteomic databases

    PaxDbi Q24JJ8.
    PRIDEi Q24JJ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G14150.1 ; AT3G14150.1 ; AT3G14150 .
    AT3G14150.2 ; AT3G14150.2 ; AT3G14150 .
    GeneIDi 820632.
    KEGGi ath:AT3G14150.

    Organism-specific databases

    TAIRi AT3G14150.

    Phylogenomic databases

    eggNOGi COG1304.
    HOGENOMi HOG000217463.
    InParanoidi Q24JJ8.
    KOi K11517.
    OMAi YVQHAEA.
    PhylomeDBi Q24JJ8.

    Enzyme and pathway databases

    UniPathwayi UPA00951 ; UER00912 .
    BioCyci ARA:AT3G14150-MONOMER.
    ARA:GQT-2460-MONOMER.

    Miscellaneous databases

    PROi Q24JJ8.

    Gene expression databases

    Genevestigatori Q24JJ8.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
      Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
      J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiGLO3_ARATH
    AccessioniPrimary (citable) accession number: Q24JJ8
    Secondary accession number(s): Q8L8P3, Q9LJH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3