Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 - Arabidopsis thaliana (Mouse-ear cress)

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Q24JJ8 (GLO3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO3
EC=1.1.3.15

Alternative name(s):
Glycolate oxidase 3
Short name=AtGLO3
Short name=GOX 3
Short chain alpha-hydroxy acid oxidase GLO3

Gene names

Name:	GLO3
Ordered Locus Names:	At3g14150
ORF Names:	MAG2.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-2-hydroxy acid + O₂ = 2-oxo acid + H₂O₂.
Cofactor	FMN By similarity.
Pathway	Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.
Subunit structure	Homotetramer or homooctamer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.
Sequence caution	The sequence BAB02979.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response signaling pathway, resistance gene-independent Inferred from mutant phenotype PubMed 22286136. Source: TAIR defense response to bacterium Inferred from mutant phenotype PubMed 22286136. Source: TAIR hydrogen peroxide biosynthetic process Inferred from mutant phenotype PubMed 22286136. Source: TAIR oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	peroxisome Inferred from direct assay PubMed 19329564. Source: TAIR
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Inferred from electronic annotation. Source: EC medium-chain-(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC very-long-chain-(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 363

363

Peroxisomal (S)-2-hydroxy-acid oxidase GLO3

PRO_0000403406

Regions

Domain

1 – 357

357

FMN hydroxy acid dehydrogenase

Nucleotide binding

283 – 307

FMN By similarity

Motif

361 – 363

Microbody targeting signal Potential

Sites

Active site

252

Proton acceptor By similarity

Binding site

107

FMN By similarity

Binding site

128

FMN By similarity

Binding site

130

Substrate By similarity

Binding site

156

FMN By similarity

Binding site

165

Substrate By similarity

Binding site

228

FMN By similarity

Binding site

255

Substrate Potential

Experimental info

Sequence conflict

P → L in AAM67194. Ref.4

Sequence conflict

309

I → M in AAM67194. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q24JJ8 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 8AA94B1A354BA60C
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FASTA

363

40,130

        10         20         30         40         50         60 
MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP RVLVDVSKID 

        70         80         90        100        110        120 
MSTKILGYPI SAPIMIAPTG NHKLAHPEGE TATAKAAAAC NTIMIVSYMS SCTFEEIASS 

       130        140        150        160        170        180 
CNAVRFLQIY VYKRRDITAQ VVKRAEKAGF KAIVLTVDVP RLGRREADIK NKMISPQLKN 

       190        200        210        220        230        240 
FEGLFSTEVR PSKGSGVQAF ASRAFDASFS WKDIEWLRSI TELPILVKGI LTREDALKAV 

       250        260        270        280        290        300 
EAGVDGIIVS NHGGRQLDYS PATITVLEEV VQVVRGRIPV LLDGGVRRGT DVFKALALGA 

       310        320        330        340        350        360 
QAVLIGRPII YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDITRN HVRTENERLH 


SML

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice." Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X. J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000600 Genomic DNA. Translation: BAB02979.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE75477.1. CP002686 Genomic DNA. Translation: AEE75478.1. BT024891 mRNA. Translation: ABD85162.1. AY088888 mRNA. Translation: AAM67194.1.
IPI	IPI00531259.
RefSeq	NP_001078152.1. NM_001084683.1. NP_188031.1. NM_112271.3.
UniGene	At.39282.
3D structure databases
ProteinModelPortal	Q24JJ8.
SMR	Q24JJ8. Positions 3-355.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G14150.1-P.
Proteomic databases
PaxDb	Q24JJ8.
PRIDE	Q24JJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G14150.1; AT3G14150.1; AT3G14150. AT3G14150.2; AT3G14150.2; AT3G14150.
GeneID	820632.
KEGG	ath:AT3G14150.
Organism-specific databases
TAIR	At3g14150.
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HOG000217463.
InParanoid	Q24JJ8.
KO	K11517.
OMA	DEWFETV.
PhylomeDB	Q24JJ8.
ProtClustDB	PLN02535.
Enzyme and pathway databases
UniPathway	UPA00951; UER00912.
Gene expression databases
Genevestigator	Q24JJ8.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO3_ARATH

Accession

Primary (citable) accession number: Q24JJ8
Secondary accession number(s): Q8L8P3, Q9LJH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	April 18, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents