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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO3

Gene

GLO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107FMNPROSITE-ProRule annotation1
Binding sitei128FMNPROSITE-ProRule annotation1
Binding sitei130SubstratePROSITE-ProRule annotation1
Binding sitei156FMNPROSITE-ProRule annotation1
Binding sitei165SubstratePROSITE-ProRule annotation1
Binding sitei228FMNPROSITE-ProRule annotation1
Active sitei252Proton acceptorPROSITE-ProRule annotation1
Binding sitei255SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 307FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • defense response to bacterium Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolate pathway, Photorespiration
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14150-MONOMER
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
R-ATH-390918 Peroxisomal lipid metabolism
UniPathwayiUPA00951; UER00912

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 3
Short name:
AtGLO3
Short name:
GOX 3
Short chain alpha-hydroxy acid oxidase GLO3
Gene namesi
Name:GLO3
Ordered Locus Names:At3g14150
ORF Names:MAG2.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G14150
TAIRilocus:2087517 AT3G14150

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034061 – 363Peroxisomal (S)-2-hydroxy-acid oxidase GLO3Add BLAST363

Proteomic databases

PaxDbiQ24JJ8
PRIDEiQ24JJ8

Expressioni

Gene expression databases

ExpressionAtlasiQ24JJ8 baseline and differential
GenevisibleiQ24JJ8 AT

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G14150.1

Structurei

3D structure databases

ProteinModelPortaliQ24JJ8
SMRiQ24JJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 357FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST357

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi361 – 363Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538 Eukaryota
COG1304 LUCA
HOGENOMiHOG000217463
InParanoidiQ24JJ8
KOiK11517
OMAiTTMKMCG
OrthoDBiEOG09360EDE
PhylomeDBiQ24JJ8

Family and domain databases

CDDicd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012133 Alpha-hydoxy_acid_DH_FMN
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
PfamiView protein in Pfam
PF01070 FMN_dh, 1 hit
PIRSFiPIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEiView protein in PROSITE
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q24JJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP
60 70 80 90 100
RVLVDVSKID MSTKILGYPI SAPIMIAPTG NHKLAHPEGE TATAKAAAAC
110 120 130 140 150
NTIMIVSYMS SCTFEEIASS CNAVRFLQIY VYKRRDITAQ VVKRAEKAGF
160 170 180 190 200
KAIVLTVDVP RLGRREADIK NKMISPQLKN FEGLFSTEVR PSKGSGVQAF
210 220 230 240 250
ASRAFDASFS WKDIEWLRSI TELPILVKGI LTREDALKAV EAGVDGIIVS
260 270 280 290 300
NHGGRQLDYS PATITVLEEV VQVVRGRIPV LLDGGVRRGT DVFKALALGA
310 320 330 340 350
QAVLIGRPII YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDITRN
360
HVRTENERLH SML
Length:363
Mass (Da):40,130
Last modified:April 18, 2006 - v1
Checksum:i8AA94B1A354BA60C
GO

Sequence cautioni

The sequence BAB02979 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87P → L in AAM67194 (Ref. 4) Curated1
Sequence conflicti309I → M in AAM67194 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA Translation: BAB02979.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE75477.1
CP002686 Genomic DNA Translation: AEE75478.1
BT024891 mRNA Translation: ABD85162.1
AY088888 mRNA Translation: AAM67194.1
RefSeqiNP_001078152.1, NM_001084683.2
NP_188031.1, NM_112271.4
UniGeneiAt.39282

Genome annotation databases

EnsemblPlantsiAT3G14150.1; AT3G14150.1; AT3G14150
AT3G14150.2; AT3G14150.2; AT3G14150
GeneIDi820632
GrameneiAT3G14150.1; AT3G14150.1; AT3G14150
AT3G14150.2; AT3G14150.2; AT3G14150
KEGGiath:AT3G14150

Similar proteinsi

Entry informationi

Entry nameiGLO3_ARATH
AccessioniPrimary (citable) accession number: Q24JJ8
Secondary accession number(s): Q8L8P3, Q9LJH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 18, 2006
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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