Reviewed, UniProtKB/Swiss-Prot Q24857 (ADH3_ENTHI)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 3
      Short name=ADH
    EC=1.1.1.2

Gene names

Name:

ADH3

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADP-dependent ADH activity.
Catalytic activity	An alcohol + NADP+ = an aldehyde + NADPH.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Alcohol dehydrogenase 3		PRO_0000087836
Experimental info
	Sequence conflict	193	1	R → I in CAA88639. Ref.2
	Sequence conflict	235 – 242	8	TLENHQDY → HKKIIKTI in CAA88639. Ref.2
	Sequence conflict	273 – 283	11	Missing Ref.2
	Sequence conflict	307 – 309	3	VMR → NE in CAA88639. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q24857-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 506FBBA933631FA9	FASTA	395	43,485
	10 20 30 40 50 60 MTMLNFTYYN PVRLIYGKGS LDEIEKQHLI PEDARIMMTY GGGSIKKNGV YEEVLKHIKP 70 80 90 100 110 120 IVEFGGIEPN PSHETCIKAI KIAKENKINF LVAVGGGSII DATKYIALGM EHTYSDDPYD 130 140 150 160 170 180 ICLKGGKFKV NPAQAKIGVV LTIPATGSET NCWGVISRHA DKLKLPFNNE SVFPTWSIVD 190 200 210 220 230 240 PCFTMSLPDN QIRNGLVDSF VHCIEQYIGN YHLNPVVEAE TEGVMRTIIG VSHKTLENHQ 250 260 270 280 290 300 DYQARITFCY AATVALNMSL LCGVTLCGGA HAVGVTLCGG AHAVGHELTS YYGLAHGETL 310 320 330 340 350 360 AITTPGVMRF NKEKNAKKLI QMGEQVFGIK NSTPEAAIEA TEKWFKSIGM KTRLSEWGKG 370 380 390 KEEFETIARK FEGNPAGAHK DIDYKGCLQI LNDIY

References

[1]	"Cloning and expression of a putative alcohol dehydrogenase gene of Entamoeba histolytica and its application to immunological examination." Kimura A., Hara Y., Kimoto T., Okuno Y., Minekawa Y., Nakabayashi T. Clin. Diagn. Lab. Immunol. 3:270-274(1996) [PubMed: 8705667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: HM-1:IMSS.
[2]	"Cloning and expression of an Entamoeba histolytica NAPD+(-)dependent alcohol dehydrogenase gene." Rodriguez M.A., Baez-Camargo M., Delgadillo D.M., Orozco E. Biochim. Biophys. Acta 1306:23-26(1996) [PubMed: 8611619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HM-1:IMSS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49910 mRNA. Translation: BAA08651.1. Z48752 Genomic DNA. Translation: CAA88639.1.
PIR	S68143.
3D structure databases
SMR	Q24857. Positions 2-374.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.2. 323.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH3_ENTHI

Accession

Primary (citable) accession number: Q24857
Secondary accession number(s): Q94560

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)