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Q24803 (ADH2_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde-alcohol dehydrogenase 2

Including the following 2 domains:

  1. Alcohol dehydrogenase
    Short name=ADH
    EC=1.1.1.1
  2. Acetaldehyde dehydrogenase
    Short name=ACDH
    EC=1.2.1.10
Gene names
Name:ADH2
OrganismEntamoeba histolytica
Taxonomic identifier5759 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme has two NAD+-dependent activities: ADH and ACDH. May be a critical enzyme in the fermentative pathway.

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

Cofactor

Zinc or iron.

Subunit structure

Seems to form a rod shaped homopolymer composed of at least 20 identical subunits.

Sequence similarities

In the N-terminal section; belongs to the aldehyde dehydrogenase family.

In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.

Sequence caution

The sequence CAA54388.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Aldehyde-alcohol dehydrogenase 2
PRO_0000087835

Regions

Nucleotide binding431 – 4366NAD Potential
Compositional bias54 – 596Poly-Ala

Sites

Active site2521 By similarity

Experimental info

Sequence conflict181R → A in CAA54388. Ref.2
Sequence conflict3801R → E AA sequence Ref.1
Sequence conflict3821H → Q AA sequence Ref.1
Sequence conflict387 – 3882HS → NP AA sequence Ref.1
Sequence conflict4821A → R in CAA54388. Ref.2
Sequence conflict5341T → I in CAA54388. Ref.2
Sequence conflict5661W → R in CAA54388. Ref.2
Sequence conflict7391A → G in CAA54388. Ref.2
Sequence conflict8211K → Q in CAA54388. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q24803 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 66733AD08CE3D0A5

FASTA87095,659
        10         20         30         40         50         60 
MSTQQTMTVD EHINQLVRKA QVALKEYLKP EYTQEKIDYI VKKASVAALD QHCALAAAAV 

        70         80         90        100        110        120 
EETGRGIFED KATKNIFACE HVTHEMRHAK TVGIINVDPL YGITEIAEPV GVVCGVTPVT 

       130        140        150        160        170        180 
NPTSTAIFKS LISIKTRNPI VFSFHPSALK CSIMAAKIVR DAAIAAGAPE NCIQWIEFGG 

       190        200        210        220        230        240 
IEASNKLMNH PGVATILATG GNAMVKAAYS SGKPALGVGA GNVPTYIEKT CNIKQAANDV 

       250        260        270        280        290        300 
VMSKSFDNGM ICASEQAAII DKEIYDQVVE EMKTLGAYFI NEEEKAKLEK FMFGVNAYSA 

       310        320        330        340        350        360 
DVNNARLNPK CPGMSPQWFA EQVGIKVPED CNIICAVCKE VGPNEPLTRE KLSPVLAILK 

       370        380        390        400        410        420 
AENTQDGIDK AEAMVEFNGR GHSAAIHSND KAVVEKYALT MKACRILHNT PSSQGGIGSI 

       430        440        450        460        470        480 
YNYIWPSFTL GCGSYGGNSV SANVTYHNLL NIKRLADRRN NLQWFRVPPK IFFEPHSIRY 

       490        500        510        520        530        540 
LAELKELSKI FIVSDRMMYK LGYVDRVMDV LKRRSNEVEI EIFIDVEPDP SIQTVQKGLA 

       550        560        570        580        590        600 
VMNTFGPDNI IAIGGGSAMD AAKIMWLLYE HPEADFFAMK QKFIDLRKRA FKFPTMGKKA 

       610        620        630        640        650        660 
RLICIPTTSG TGSEVTPFAV ISDHETGKKY PLADYSLTPS VAIVDPMFTM SLPKRAIADT 

       670        680        690        700        710        720 
GLDVLVHATE AYVSVMANEY TDGLAREAVK LVFENLLKSY NGDLEAREKM HNAATIAGMA 

       730        740        750        760        770        780 
FASAFLGMDH SMAHKVGAAF HLPHGRCVAV LLPHVIRYNG QKPRKLAMWP KYNFYKADQR 

       790        800        810        820        830        840 
YMELAQMVGL KCNTPAEGVE AFAKACEELM KATETITGFK KANIDEAAWM SKVPEMALLA 

       850        860        870 
FEDQCSPANP RVPMVKDMEK ILKAAYYPIA 

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References

[1]"Entamoeba histolytica has an alcohol dehydrogenase homologous to the multifunctional adhE gene product of Escherichia coli."
Yang W., Li E., Kairong T., Stanley S.L. Jr.
Mol. Biochem. Parasitol. 64:253-260(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 377-395 AND 860-870.
Strain: ATCC 30459 / HM-1:IMSS.
[2]"Purification and molecular characterization of the NAD(+)-dependent acetaldehyde/alcohol dehydrogenase from Entamoeba histolytica."
Bruchhaus I., Tannich E.
Biochem. J. 303:743-748(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ATCC 30459 / HM-1:IMSS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04863 mRNA. Translation: AAA81906.1.
X77132 mRNA. Translation: CAA54388.1. Different initiation.
PIRS53319.

3D structure databases

ProteinModelPortalQ24803.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG1012.

Family and domain databases

Gene3D3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald/ADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMSSF53720. SSF53720. 2 hits.
PROSITEPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADH2_ENTHI
AccessionPrimary (citable) accession number: Q24803
Secondary accession number(s): Q27649
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 16, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families