Reviewed, UniProtKB/Swiss-Prot Q24708 (CSW_DROVI)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein phosphatase corkscrew
    EC=3.1.3.48

Gene names

Name:

csw

Organism

Drosophila virilis (Fruit fly)

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	764 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required in all receptor tyrosine kinase signaling pathways. Functions downstream of the receptor tyrosine kinase torso, acting in concert with D-Raf via tailless. Also functions downstream of Egfr (epidermal growth factor receptor) and btl (fibroblast growth factor receptor). The SH2 domain suggests that csw effects its role by mediating heteromeric protein interactions. Maternally required for normal determination of cell fates at the termini of the embryo. Required for cell fate specification of the ventral ectoderm, in the developing embryonic CNS and for embryonic tracheal cell migration. Functions during imaginal development for proper formation of adult structures such as eyes, aristae, L5 wing vein and the tarsal claw By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains at least 1 SH2 domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	SH2 domain
Molecular function	Developmental protein Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro terminal region determination Inferred from sequence or structural similarity. Source: UniProtKB torso signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 764	›764	Tyrosine-protein phosphatase corkscrew		PRO_0000094852
Regions
	Domain	1 – 95	95	SH2
	Domain	117 – 522	406	Tyrosine-protein phosphatase
	Region	174 – 325	152	PTPase insert (Cys/Ser-rich)
	Compositional bias	186 – 191	6	Poly-Ser
	Compositional bias	278 – 281	4	Poly-Ala
	Compositional bias	576 – 600	25	Ala-rich
	Compositional bias	613 – 616	4	Poly-Asn
	Compositional bias	617 – 656	40	Ser-rich
	Compositional bias	687 – 690	4	Poly-Gln
	Compositional bias	694 – 753	60	Ala-rich
	Compositional bias	755 – 761	7	Poly-Pro
Sites
	Active site	460	1	Phosphocysteine intermediate By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q24708-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 66008EA8560A2F7D	FASTA	764	82,126
	10 20 30 40 50 60 WFHGNLSGKE AEKLILERGK NGSFLVRESQ SKPGDFVLSV RTDDKVTHVM IRWQDKKYDV 70 80 90 100 110 120 GGGESFATLS ELIEHYKRHP MVETCGTVVH LRQPFNATRI TAAGINARVE QLVKGGFWEE 130 140 150 160 170 180 FESLQQDSRD TFSRHEGYKD ENRLKNRYHD HTRVKLQDVE RSAPGAEYIN ANYIRLPTDG 190 200 210 220 230 240 DLYNMSSSSE SLNSTVAACP ACTAAQTQRN CPNCHLLNKT CVKCAVKSAT LPTNCATCNR 250 260 270 280 290 300 KSDSLSKHKR SESMSASANA SAAGTGPGTP TAAGNTSAAA ALNGCLAVLL KKHCGDASPP 310 320 330 340 350 360 PSTTSSCSGP LTGSLLNGEG NQFKTYIATQ GCLANTKTDF WNMIWQENTR VIVMTTKEIE 370 380 390 400 410 420 RGKTKCERYW PDEGQCKQFG HAKVHCIKEN STNDYTLREF LFSWRDKPER RIYHYHFQVW 430 440 450 460 470 480 PDHGVPADPG CVLNFLQDVN TKQSSLAQAG EKPGPICVHC SAGIGRTGTF IVIDMILDQI 490 500 510 520 530 540 VRNGLDTEID IQRTIQMVRS QRSGMVQTEA QYKFVYYAVQ HYIQTLIARK RAEEQSLQVG 550 560 570 580 590 600 REYTNIKYTG EIGNDSQRSP LPPAISNLSL VSCKSAVAEP LTAAAAAAAV AANAGNKHAA 610 620 630 640 650 660 KLQPPLPPLG ASNNNNSSGN SGSYCNSSSS TSTAQHNGVV SSSNNCSSGS GSANSSNANG 670 680 690 700 710 720 NGNILGNGSN MRKSNFYSDS LAALKLQQQQ LHDAATAAAA AALASAAAPA ATTTAASASA 730 740 750 760 AAAAAAAAKY KNIPKDMNSL RQPHAAYVAA APALPPPPTP PRKT

References

[1]	"The role of the Drosophila corkscrew protein as a transducer downstream of receptor tyrosine kinases is functionally conserved." Melnick M.B., Melnick C.B., Larsen I., Perrimon N., Perkins L.A. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U22356 Genomic DNA. Translation: AAB02545.1.
3D structure databases
HSSP	HSSP built from PDB template 2SHP based on UniProtKB Q06124.
SMR	Q24708. Positions 1-174.
ModBase	Search...
Organism-specific databases
FlyBase	FBgn0016496. Dvir\csw.
Enzyme and pathway databases
BRENDA	3.1.3.48. 141708.
Family and domain databases
InterPro	IPR000387. Dual-sp/Tyr_phosphatase. IPR000980. SH2. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE. PR00401. SH2DOMAIN.
ProDom	PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00194. PTPc. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS50001. SH2. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSW_DROVI

Accession

Primary (citable) accession number: Q24708

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	November 1, 1996
Last modified:	July 28, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project