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Q24574

- UBPE_DROME

UniProt

Q24574 - UBPE_DROME

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Protein

Ubiquitin carboxyl-terminal hydrolase 64E

Gene

Ubp64E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has an effect on position-effect variegation.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophilePROSITE-ProRule annotation
Active sitei720 – 7201Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. compound eye cone cell differentiation Source: FlyBase
  2. protein deubiquitination Source: FlyBase
  3. protein stabilization Source: FlyBase
  4. regulation of chromatin silencing Source: FlyBase
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.105.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 64E (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase 64E
Ubiquitin-specific-processing protease 64E
Short name:
Deubiquitinating enzyme 64E
Gene namesi
Name:Ubp64E
Synonyms:D-UBP-64E
ORF Names:CG5486
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0016756. Ubp64E.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15561556Ubiquitin carboxyl-terminal hydrolase 64EPRO_0000080687Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei172 – 1721Phosphoserine1 Publication
Modified residuei173 – 1731Phosphoserine1 Publication
Modified residuei238 – 2381Phosphoserine1 Publication
Modified residuei1131 – 11311Phosphoserine1 Publication
Modified residuei1132 – 11321Phosphoserine1 Publication
Modified residuei1140 – 11401Phosphoserine1 Publication
Modified residuei1141 – 11411Phosphoserine1 Publication
Modified residuei1199 – 11991Phosphoserine1 Publication
Modified residuei1201 – 12011Phosphoserine1 Publication
Modified residuei1205 – 12051Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24574.

Expressioni

Gene expression databases

BgeeiQ24574.

Interactioni

Protein-protein interaction databases

BioGridi64105. 7 interactions.
IntActiQ24574. 4 interactions.
MINTiMINT-971166.

Structurei

3D structure databases

ProteinModelPortaliQ24574.
SMRiQ24574. Positions 394-812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini396 – 779384USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
InParanoidiQ24574.
KOiK11857.
OMAiLECNTEK.
OrthoDBiEOG7M3HZD.
PhylomeDBiQ24574.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q24574-1) [UniParc]FASTAAdd to Basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDKESEQCT VSVFDQTPGS EQKKINVVVR SHFTVKRVID LIGTQFSYEK
60 70 80 90 100
FELLLQPHDN KDLVNLNALE SQLMYEVAGF EPQLKNHLIL LPSGSWDGDV
110 120 130 140 150
TKRFELPIKR VVVKKVMKSD GEKAKSPATG EKKKRVVGEK TKKKPASGSS
160 170 180 190 200
SPSKAKTTSE DSLAKTSISS ESSPEKTSKI KTTAAKISKP GSEKAPRASP
210 220 230 240 250
EECPELSTEI NSKNTSSESP VAKKTAKVTS KPTLELLSPI KPSSPIKELD
260 270 280 290 300
CEPVDTLSKQ QLSEQLQLYP QGRNLISPVD DAPSDLFISD AEQLSDDDLA
310 320 330 340 350
LGASASPTML GPGYDYGAPT GDSDVEGVTG VTDPSTIGTD DGTYPALSNF
360 370 380 390 400
YRRKYGGDEL RAWQRVNTTG ADFVSSATTE TEAEARQASL GPRGYVGLVN
410 420 430 440 450
QAMTCYLNSL LQALFMTPEF RNALYRWEFD NDNEAKNIPY QLQKLFLNLQ
460 470 480 490 500
TSPKAAVETT DLTRSFGWDS TEAWQQHDIQ ELCRVMFDAL EHKFKNTKQA
510 520 530 540 550
NLISNLYEGK MNDYVKCLEC NTEKTREDTF LDIPLPVRPF GSSSAYGSIE
560 570 580 590 600
EALRAFVQPE TLDGNNQYLC EKCKKKCDAH KGLHFKSFPY ILTLHLKRFD
610 620 630 640 650
FDYQTMHRIK LNDRVTFPQT LNLNTFINRS GNSGEQNSQL NGTVDDCSTA
660 670 680 690 700
DSGSAMEDDN LSSGVVTTAS SSQHENDLND EDEGIDMSSS TSKSAKQGSG
710 720 730 740 750
PYLYELFAIM IHSGSASGGH YYAYIKDFDN NEWFCFNDQN VTSITQEDIQ
760 770 780 790 800
RSFGGPNGSY YSSAYTSSTN AYMLMYRQVD AKRNELVAKV ADFPEHIKTL
810 820 830 840 850
LPKLHSEEET RVSRLGRHIT VTDLALPDLY KPRVYFYNPS LKKMKITRVY
860 870 880 890 900
VSQSFNINLV LMSAYEMLNV EQFAPLSRCR LVAYNSSMDT IIQSLESCTD
910 920 930 940 950
PALTELRAAQ NYSLDFLLEY RAEDQEFEVY PPNGITWYVF KVDLSTMAMD
960 970 980 990 1000
GPFLVYSAAR EREASDVLRR SIALRLHISE QQFLLATVRA TVPKAFVSYD
1010 1020 1030 1040 1050
PHPTPEALQH LQNMANTQFK SITYFYLNVP NTDAATLEML GVPTVESVEC
1060 1070 1080 1090 1100
ASGGDVVDAA MMNGVAPGHM SSSNDYDWRR YKRDLVEPMS QPSPSHGHES
1110 1120 1130 1140 1150
NSEDSSLSDG DRTLVETDNM AHRGGGDSQV SSTSHSPQLS SPEDEAASHD
1160 1170 1180 1190 1200
AMMRVHAYCN GNGSYAAADV VDPLLLPTST NHFFYATKVE CVDVVGTGSS
1210 1220 1230 1240 1250
SGHQSDEEAQ LRKPTRAYKL LVGTHMRMGA FKKHIEQLIQ VPAAHFKLQR
1260 1270 1280 1290 1300
KHDNNLSNNQ NNSLVHLIEG ETLTVELGKT LEPDEFKAKI HFLRLADIDN
1310 1320 1330 1340 1350
ETSKLPCVCE WVYNANTTAE QAKKELVAKL HRIDAKYATL SVQNCRIWLK
1360 1370 1380 1390 1400
GGRIPIKILS DDETLYCDMR SSIAAEFIVQ ECEEEVDPQP KDDSLTLFVR
1410 1420 1430 1440 1450
RWCPAKLEFG KFQEITLDQD SEIRLSLSQI SDIPIDKLSY MKLNSNFPCT
1460 1470 1480 1490 1500
SISALSVNES SSWYSVPTTL DKYPLNSTQT GNIYLYKDRT VPARELTLEE
1510 1520 1530 1540 1550
RRLMNAREKA RLDRVGCVST TRYAQRRERA LKIYLDSPEK SSNVTASAPM

DVHVNN
Length:1,556
Mass (Da):173,794
Last modified:September 13, 2005 - v2
Checksum:iF70AE5BB2914E8BE
GO
Isoform D (identifier: Q24574-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-289: Missing.

Note: No experimental confirmation available.

Show »
Length:1,538
Mass (Da):171,896
Checksum:i497CFA02811B7D18
GO

Sequence cautioni

The sequence CAA67601.1 differs from that shown. Reason: Frameshift at several positions.
The sequence AAL13901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAA67601.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4393NIP → KS in CAA67601. (PubMed:8816485)Curated
Sequence conflicti513 – 5131D → A in CAA67601. (PubMed:8816485)Curated
Sequence conflicti519 – 5191E → G in CAA67601. (PubMed:8816485)Curated
Sequence conflicti525 – 5262TR → NDC in CAA67601. (PubMed:8816485)Curated
Sequence conflicti645 – 6451D → DS in AAL13901. (PubMed:12537569)Curated
Sequence conflicti791 – 7911A → R in CAA67601. (PubMed:8816485)Curated
Sequence conflicti910 – 9101Missing in CAA67601. (PubMed:8816485)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei272 – 28918Missing in isoform D. CuratedVSP_054033Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014296 Genomic DNA. Translation: AAF50752.2.
AE014296 Genomic DNA. Translation: AAN12105.1.
AE014296 Genomic DNA. Translation: AAS65076.2.
BT003784 mRNA. Translation: AAO41467.1.
X99211 mRNA. Translation: CAA67601.1. Sequence problems.
AY058672 mRNA. Translation: AAL13901.1. Different initiation.
RefSeqiNP_523937.2. NM_079213.4. [Q24574-1]
NP_729087.1. NM_168129.4. [Q24574-1]
NP_996001.2. NM_206279.4. [Q24574-2]
UniGeneiDm.3704.

Genome annotation databases

EnsemblMetazoaiFBtr0077095; FBpp0076802; FBgn0016756. [Q24574-1]
FBtr0077096; FBpp0076803; FBgn0016756. [Q24574-1]
GeneIDi38644.
KEGGidme:Dmel_CG5486.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014296 Genomic DNA. Translation: AAF50752.2 .
AE014296 Genomic DNA. Translation: AAN12105.1 .
AE014296 Genomic DNA. Translation: AAS65076.2 .
BT003784 mRNA. Translation: AAO41467.1 .
X99211 mRNA. Translation: CAA67601.1 . Sequence problems.
AY058672 mRNA. Translation: AAL13901.1 . Different initiation.
RefSeqi NP_523937.2. NM_079213.4. [Q24574-1 ]
NP_729087.1. NM_168129.4. [Q24574-1 ]
NP_996001.2. NM_206279.4. [Q24574-2 ]
UniGenei Dm.3704.

3D structure databases

ProteinModelPortali Q24574.
SMRi Q24574. Positions 394-812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 64105. 7 interactions.
IntActi Q24574. 4 interactions.
MINTi MINT-971166.

Protein family/group databases

MEROPSi C19.105.

Proteomic databases

PaxDbi Q24574.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0077095 ; FBpp0076802 ; FBgn0016756 . [Q24574-1 ]
FBtr0077096 ; FBpp0076803 ; FBgn0016756 . [Q24574-1 ]
GeneIDi 38644.
KEGGi dme:Dmel_CG5486.

Organism-specific databases

CTDi 38644.
FlyBasei FBgn0016756. Ubp64E.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00760000119158.
InParanoidi Q24574.
KOi K11857.
OMAi LECNTEK.
OrthoDBi EOG7M3HZD.
PhylomeDBi Q24574.

Miscellaneous databases

ChiTaRSi Ubp64E. drosophila.
GenomeRNAii 38644.
NextBioi 809673.
PROi Q24574.

Gene expression databases

Bgeei Q24574.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  4. "The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster."
    Henchoz S., de Rubertis F., Pauli D., Spierer P.
    Mol. Cell. Biol. 16:5717-5725(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 318-1556 (ISOFORMS A/D).
    Tissue: Embryo.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-1556 (ISOFORMS A/D).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172; SER-173; SER-238; SER-1131; SER-1132; SER-1140; SER-1141; SER-1199; SER-1201 AND SER-1205, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiUBPE_DROME
AccessioniPrimary (citable) accession number: Q24574
Secondary accession number(s): A4V1I7, Q95TM9, Q9VRP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3