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Protein

Ubiquitin carboxyl-terminal hydrolase 47

Gene

Usp47

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-specific protease that deubiquitinates target proteins to regulate different cellular and developmental pathways (PubMed:10949024, PubMed:18160715, PubMed:26169834, PubMed:27552662). Functions downstream of Dsor1/MEK to positively regulate the Ras/MAPK signaling pathway (PubMed:27552662). Likely to modulate the pathway during various cellular and developmental processes including rl/MAPK activation by the receptors InR, Egfr and sevenless/sev (PubMed:27552662). Functions in the post-translational stabilization of rl/MAPK levels in a mechanism that is independent of rl activity and opposes the activity of the E2 enzyme Unc6 and the putative E3 ligases poe, Ufd4 and Kcmf1, which mediate the ubiquitination and proteasomal degradation of rl (PubMed:27552662). During eye development it may also act downstream of rl/MAPK to negatively regulate the Ras/MAPK signaling pathway by stabilizing the transcriptional repressor ttk and consequently inhibiting photoreceptor cell development (PubMed:18160715). This suggests that at least during eye development, it may act in both the positive and negative regulation of the Ras/MAPK signaling pathway to mediate the development of different cell types (PubMed:18160715, PubMed:27552662). Positively regulates border follicle cell migration during oogenesis by mediating the deubiquitination and stabilization of slbo (PubMed:10949024). In the wing disks it positively regulates wg signaling by stabilizing arm (PubMed:26169834). Has an effect on position-effect variegation (PubMed:8816485).5 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei405NucleophilePROSITE-ProRule annotation2 Publications1
Active sitei720Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  • base-excision repair Source: GO_Central
  • compound eye cone cell differentiation Source: FlyBase
  • negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • protein deubiquitination Source: FlyBase
  • protein stabilization Source: FlyBase
  • regulation of apoptotic process Source: GO_Central
  • regulation of chromatin silencing Source: FlyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC19.105

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 47By similarity (EC:3.4.19.123 Publications)
Alternative name(s):
Ubiquitin thioesterase 47By similarity
Ubiquitin-specific-processing protease 471 Publication
Gene namesi
Name:Usp471 PublicationImported
Synonyms:Ubp64E1 Publication
ORF Names:CG5486Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0016756 Usp47

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in the adult wing results in the development of wing margin bristles (PubMed:26169834). RNAi-mediated knockdown in the posterior compartment of the wing reduces accumulation of arm, resulting in reduced expression of sens and wg (PubMed:26169834). RNAi-mediated knockdown in larval wing or eye imaginal disks results in decreased rl protein levels (PubMed:27552662).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi405C → A: Loss of activity. 1 Publication1
Mutagenesisi405C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806871 – 1556Ubiquitin carboxyl-terminal hydrolase 47Add BLAST1556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei172Phosphoserine1 Publication1
Modified residuei173Phosphoserine1 Publication1
Modified residuei238Phosphoserine1 Publication1
Modified residuei1131Phosphoserine1 Publication1
Modified residuei1132Phosphoserine1 Publication1
Modified residuei1140Phosphoserine1 Publication1
Modified residuei1141Phosphoserine1 Publication1
Modified residuei1199Phosphoserine1 Publication1
Modified residuei1201Phosphoserine1 Publication1
Modified residuei1205Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24574
PRIDEiQ24574

PTM databases

iPTMnetiQ24574

Expressioni

Developmental stagei

Ubiquitously expressed in the embryo (at protein level). Ubiquitously expressed in the developing eye (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0016756
GenevisibleiQ24574 DM

Interactioni

Subunit structurei

Interacts with ttk.1 Publication

Protein-protein interaction databases

BioGridi64105, 10 interactors
IntActiQ24574, 4 interactors
STRINGi7227.FBpp0076802

Structurei

3D structure databases

ProteinModelPortaliQ24574
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini396 – 779USPPROSITE-ProRule annotationAdd BLAST384

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG4598 Eukaryota
COG5077 LUCA
GeneTreeiENSGT00760000119158
InParanoidiQ24574
KOiK11857
OMAiDCDWRRY
OrthoDBiEOG091G0173
PhylomeDBiQ24574

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
SUPFAMiSSF54001 SSF54001, 2 hits
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AImported (identifier: Q24574-1) [UniParc]FASTAAdd to basket
Also known as: BImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDKESEQCT VSVFDQTPGS EQKKINVVVR SHFTVKRVID LIGTQFSYEK
60 70 80 90 100
FELLLQPHDN KDLVNLNALE SQLMYEVAGF EPQLKNHLIL LPSGSWDGDV
110 120 130 140 150
TKRFELPIKR VVVKKVMKSD GEKAKSPATG EKKKRVVGEK TKKKPASGSS
160 170 180 190 200
SPSKAKTTSE DSLAKTSISS ESSPEKTSKI KTTAAKISKP GSEKAPRASP
210 220 230 240 250
EECPELSTEI NSKNTSSESP VAKKTAKVTS KPTLELLSPI KPSSPIKELD
260 270 280 290 300
CEPVDTLSKQ QLSEQLQLYP QGRNLISPVD DAPSDLFISD AEQLSDDDLA
310 320 330 340 350
LGASASPTML GPGYDYGAPT GDSDVEGVTG VTDPSTIGTD DGTYPALSNF
360 370 380 390 400
YRRKYGGDEL RAWQRVNTTG ADFVSSATTE TEAEARQASL GPRGYVGLVN
410 420 430 440 450
QAMTCYLNSL LQALFMTPEF RNALYRWEFD NDNEAKNIPY QLQKLFLNLQ
460 470 480 490 500
TSPKAAVETT DLTRSFGWDS TEAWQQHDIQ ELCRVMFDAL EHKFKNTKQA
510 520 530 540 550
NLISNLYEGK MNDYVKCLEC NTEKTREDTF LDIPLPVRPF GSSSAYGSIE
560 570 580 590 600
EALRAFVQPE TLDGNNQYLC EKCKKKCDAH KGLHFKSFPY ILTLHLKRFD
610 620 630 640 650
FDYQTMHRIK LNDRVTFPQT LNLNTFINRS GNSGEQNSQL NGTVDDCSTA
660 670 680 690 700
DSGSAMEDDN LSSGVVTTAS SSQHENDLND EDEGIDMSSS TSKSAKQGSG
710 720 730 740 750
PYLYELFAIM IHSGSASGGH YYAYIKDFDN NEWFCFNDQN VTSITQEDIQ
760 770 780 790 800
RSFGGPNGSY YSSAYTSSTN AYMLMYRQVD AKRNELVAKV ADFPEHIKTL
810 820 830 840 850
LPKLHSEEET RVSRLGRHIT VTDLALPDLY KPRVYFYNPS LKKMKITRVY
860 870 880 890 900
VSQSFNINLV LMSAYEMLNV EQFAPLSRCR LVAYNSSMDT IIQSLESCTD
910 920 930 940 950
PALTELRAAQ NYSLDFLLEY RAEDQEFEVY PPNGITWYVF KVDLSTMAMD
960 970 980 990 1000
GPFLVYSAAR EREASDVLRR SIALRLHISE QQFLLATVRA TVPKAFVSYD
1010 1020 1030 1040 1050
PHPTPEALQH LQNMANTQFK SITYFYLNVP NTDAATLEML GVPTVESVEC
1060 1070 1080 1090 1100
ASGGDVVDAA MMNGVAPGHM SSSNDYDWRR YKRDLVEPMS QPSPSHGHES
1110 1120 1130 1140 1150
NSEDSSLSDG DRTLVETDNM AHRGGGDSQV SSTSHSPQLS SPEDEAASHD
1160 1170 1180 1190 1200
AMMRVHAYCN GNGSYAAADV VDPLLLPTST NHFFYATKVE CVDVVGTGSS
1210 1220 1230 1240 1250
SGHQSDEEAQ LRKPTRAYKL LVGTHMRMGA FKKHIEQLIQ VPAAHFKLQR
1260 1270 1280 1290 1300
KHDNNLSNNQ NNSLVHLIEG ETLTVELGKT LEPDEFKAKI HFLRLADIDN
1310 1320 1330 1340 1350
ETSKLPCVCE WVYNANTTAE QAKKELVAKL HRIDAKYATL SVQNCRIWLK
1360 1370 1380 1390 1400
GGRIPIKILS DDETLYCDMR SSIAAEFIVQ ECEEEVDPQP KDDSLTLFVR
1410 1420 1430 1440 1450
RWCPAKLEFG KFQEITLDQD SEIRLSLSQI SDIPIDKLSY MKLNSNFPCT
1460 1470 1480 1490 1500
SISALSVNES SSWYSVPTTL DKYPLNSTQT GNIYLYKDRT VPARELTLEE
1510 1520 1530 1540 1550
RRLMNAREKA RLDRVGCVST TRYAQRRERA LKIYLDSPEK SSNVTASAPM

DVHVNN
Length:1,556
Mass (Da):173,794
Last modified:September 13, 2005 - v2
Checksum:iF70AE5BB2914E8BE
GO
Isoform DImported (identifier: Q24574-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-289: Missing.

Note: No experimental confirmation available.
Show »
Length:1,538
Mass (Da):171,896
Checksum:i497CFA02811B7D18
GO

Sequence cautioni

The sequence AAL13901 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA67601 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence CAA67601 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti437 – 439NIP → KS in CAA67601 (PubMed:8816485).Curated3
Sequence conflicti513D → A in CAA67601 (PubMed:8816485).Curated1
Sequence conflicti519E → G in CAA67601 (PubMed:8816485).Curated1
Sequence conflicti525 – 526TR → NDC in CAA67601 (PubMed:8816485).Curated2
Sequence conflicti645D → DS in AAL13901 (PubMed:12537569).Curated1
Sequence conflicti791A → R in CAA67601 (PubMed:8816485).Curated1
Sequence conflicti910Missing in CAA67601 (PubMed:8816485).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054033272 – 289Missing in isoform D. CuratedAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF50752.2
AE014296 Genomic DNA Translation: AAN12105.1
AE014296 Genomic DNA Translation: AAS65076.2
BT003784 mRNA Translation: AAO41467.1
X99211 mRNA Translation: CAA67601.1 Sequence problems.
AY058672 mRNA Translation: AAL13901.1 Different initiation.
RefSeqiNP_523937.2, NM_079213.4 [Q24574-1]
NP_729087.1, NM_168129.4 [Q24574-1]
NP_996001.2, NM_206279.4 [Q24574-2]
UniGeneiDm.3704

Genome annotation databases

EnsemblMetazoaiFBtr0077095; FBpp0076802; FBgn0016756 [Q24574-1]
FBtr0077096; FBpp0076803; FBgn0016756 [Q24574-1]
FBtr0306259; FBpp0297369; FBgn0016756 [Q24574-2]
GeneIDi38644
KEGGidme:Dmel_CG5486

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBPE_DROME
AccessioniPrimary (citable) accession number: Q24574
Secondary accession number(s): A4V1I7, Q95TM9, Q9VRP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 13, 2005
Last modified: June 20, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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