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Q24574 (UBPE_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 64E

EC=3.4.19.12
Alternative name(s):
Ubiquitin thioesterase 64E
Ubiquitin-specific-processing protease 64E
Short name=Deubiquitinating enzyme 64E
Gene names
Name:Ubp64E
Synonyms:D-UBP-64E
ORF Names:CG5486
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1556 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has an effect on position-effect variegation.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence AAL13901.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAA67601.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAA67601.1 differs from that shown. Reason: Frameshift at several positions.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q24574-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform D (identifier: Q24574-2)

The sequence of this isoform differs from the canonical sequence as follows:
     272-289: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15561556Ubiquitin carboxyl-terminal hydrolase 64E
PRO_0000080687

Regions

Domain396 – 779384USP

Sites

Active site4051Nucleophile By similarity
Active site7201Proton acceptor By similarity

Amino acid modifications

Modified residue1721Phosphoserine Ref.6
Modified residue1731Phosphoserine Ref.6
Modified residue2381Phosphoserine Ref.6
Modified residue11311Phosphoserine Ref.6
Modified residue11321Phosphoserine Ref.6
Modified residue11401Phosphoserine Ref.6
Modified residue11411Phosphoserine Ref.6
Modified residue11991Phosphoserine Ref.6
Modified residue12011Phosphoserine Ref.6
Modified residue12051Phosphoserine Ref.6

Natural variations

Alternative sequence272 – 28918Missing in isoform D.
VSP_054033

Experimental info

Sequence conflict437 – 4393NIP → KS in CAA67601. Ref.4
Sequence conflict5131D → A in CAA67601. Ref.4
Sequence conflict5191E → G in CAA67601. Ref.4
Sequence conflict525 – 5262TR → NDC in CAA67601. Ref.4
Sequence conflict6451D → DS in AAL13901. Ref.5
Sequence conflict7911A → R in CAA67601. Ref.4
Sequence conflict9101Missing in CAA67601. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform A (B) [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: F70AE5BB2914E8BE

FASTA1,556173,794
        10         20         30         40         50         60 
MTDKESEQCT VSVFDQTPGS EQKKINVVVR SHFTVKRVID LIGTQFSYEK FELLLQPHDN 

        70         80         90        100        110        120 
KDLVNLNALE SQLMYEVAGF EPQLKNHLIL LPSGSWDGDV TKRFELPIKR VVVKKVMKSD 

       130        140        150        160        170        180 
GEKAKSPATG EKKKRVVGEK TKKKPASGSS SPSKAKTTSE DSLAKTSISS ESSPEKTSKI 

       190        200        210        220        230        240 
KTTAAKISKP GSEKAPRASP EECPELSTEI NSKNTSSESP VAKKTAKVTS KPTLELLSPI 

       250        260        270        280        290        300 
KPSSPIKELD CEPVDTLSKQ QLSEQLQLYP QGRNLISPVD DAPSDLFISD AEQLSDDDLA 

       310        320        330        340        350        360 
LGASASPTML GPGYDYGAPT GDSDVEGVTG VTDPSTIGTD DGTYPALSNF YRRKYGGDEL 

       370        380        390        400        410        420 
RAWQRVNTTG ADFVSSATTE TEAEARQASL GPRGYVGLVN QAMTCYLNSL LQALFMTPEF 

       430        440        450        460        470        480 
RNALYRWEFD NDNEAKNIPY QLQKLFLNLQ TSPKAAVETT DLTRSFGWDS TEAWQQHDIQ 

       490        500        510        520        530        540 
ELCRVMFDAL EHKFKNTKQA NLISNLYEGK MNDYVKCLEC NTEKTREDTF LDIPLPVRPF 

       550        560        570        580        590        600 
GSSSAYGSIE EALRAFVQPE TLDGNNQYLC EKCKKKCDAH KGLHFKSFPY ILTLHLKRFD 

       610        620        630        640        650        660 
FDYQTMHRIK LNDRVTFPQT LNLNTFINRS GNSGEQNSQL NGTVDDCSTA DSGSAMEDDN 

       670        680        690        700        710        720 
LSSGVVTTAS SSQHENDLND EDEGIDMSSS TSKSAKQGSG PYLYELFAIM IHSGSASGGH 

       730        740        750        760        770        780 
YYAYIKDFDN NEWFCFNDQN VTSITQEDIQ RSFGGPNGSY YSSAYTSSTN AYMLMYRQVD 

       790        800        810        820        830        840 
AKRNELVAKV ADFPEHIKTL LPKLHSEEET RVSRLGRHIT VTDLALPDLY KPRVYFYNPS 

       850        860        870        880        890        900 
LKKMKITRVY VSQSFNINLV LMSAYEMLNV EQFAPLSRCR LVAYNSSMDT IIQSLESCTD 

       910        920        930        940        950        960 
PALTELRAAQ NYSLDFLLEY RAEDQEFEVY PPNGITWYVF KVDLSTMAMD GPFLVYSAAR 

       970        980        990       1000       1010       1020 
EREASDVLRR SIALRLHISE QQFLLATVRA TVPKAFVSYD PHPTPEALQH LQNMANTQFK 

      1030       1040       1050       1060       1070       1080 
SITYFYLNVP NTDAATLEML GVPTVESVEC ASGGDVVDAA MMNGVAPGHM SSSNDYDWRR 

      1090       1100       1110       1120       1130       1140 
YKRDLVEPMS QPSPSHGHES NSEDSSLSDG DRTLVETDNM AHRGGGDSQV SSTSHSPQLS 

      1150       1160       1170       1180       1190       1200 
SPEDEAASHD AMMRVHAYCN GNGSYAAADV VDPLLLPTST NHFFYATKVE CVDVVGTGSS 

      1210       1220       1230       1240       1250       1260 
SGHQSDEEAQ LRKPTRAYKL LVGTHMRMGA FKKHIEQLIQ VPAAHFKLQR KHDNNLSNNQ 

      1270       1280       1290       1300       1310       1320 
NNSLVHLIEG ETLTVELGKT LEPDEFKAKI HFLRLADIDN ETSKLPCVCE WVYNANTTAE 

      1330       1340       1350       1360       1370       1380 
QAKKELVAKL HRIDAKYATL SVQNCRIWLK GGRIPIKILS DDETLYCDMR SSIAAEFIVQ 

      1390       1400       1410       1420       1430       1440 
ECEEEVDPQP KDDSLTLFVR RWCPAKLEFG KFQEITLDQD SEIRLSLSQI SDIPIDKLSY 

      1450       1460       1470       1480       1490       1500 
MKLNSNFPCT SISALSVNES SSWYSVPTTL DKYPLNSTQT GNIYLYKDRT VPARELTLEE 

      1510       1520       1530       1540       1550 
RRLMNAREKA RLDRVGCVST TRYAQRRERA LKIYLDSPEK SSNVTASAPM DVHVNN 

« Hide

Isoform D [UniParc].

Checksum: 497CFA02811B7D18
Show »

FASTA1,538171,896

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[4]"The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster."
Henchoz S., de Rubertis F., Pauli D., Spierer P.
Mol. Cell. Biol. 16:5717-5725(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 318-1556 (ISOFORMS A/D).
Tissue: Embryo.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-1556 (ISOFORMS A/D).
Strain: Berkeley.
Tissue: Embryo.
[6]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172; SER-173; SER-238; SER-1131; SER-1132; SER-1140; SER-1141; SER-1199; SER-1201 AND SER-1205, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014296 Genomic DNA. Translation: AAF50752.2.
AE014296 Genomic DNA. Translation: AAN12105.1.
AE014296 Genomic DNA. Translation: AAS65076.2.
BT003784 mRNA. Translation: AAO41467.1.
X99211 mRNA. Translation: CAA67601.1. Sequence problems.
AY058672 mRNA. Translation: AAL13901.1. Different initiation.
RefSeqNP_523937.2. NM_079213.4.
NP_729087.1. NM_168129.3.
NP_996001.2. NM_206279.3.
UniGeneDm.3704.

3D structure databases

ProteinModelPortalQ24574.
SMRQ24574. Positions 393-812.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid64105. 7 interactions.
IntActQ24574. 4 interactions.
MINTMINT-971166.

Protein family/group databases

MEROPSC19.105.

Proteomic databases

PaxDbQ24574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0077095; FBpp0076802; FBgn0016756.
FBtr0077096; FBpp0076803; FBgn0016756.
GeneID38644.
KEGGdme:Dmel_CG5486.

Organism-specific databases

CTD38644.
FlyBaseFBgn0016756. Ubp64E.

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00740000115066.
InParanoidQ24574.
KOK11857.
OMALECNTEK.
OrthoDBEOG7M3HZD.
PhylomeDBQ24574.

Gene expression databases

BgeeQ24574.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUbp64E. drosophila.
GenomeRNAi38644.
NextBio809673.
PROQ24574.

Entry information

Entry nameUBPE_DROME
AccessionPrimary (citable) accession number: Q24574
Secondary accession number(s): A4V1I7, Q95TM9, Q9VRP1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase