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Protein

Probable histone-binding protein Caf1

Gene

Caf1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the nucleosome remodeling and deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the nucleosome remodeling factor (NURF) complex, which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin; and the polycomb group (PcG) repressor complex ESC-E(Z), which promotes repression of homeotic genes during development. Also required for transcriptional repression of E2F target genes by E2f2 and Rbf or Rbf2.5 Publications

GO - Molecular functioni

  • histone acetyltransferase binding Source: FlyBase
  • histone binding Source: FlyBase
  • histone deacetylase binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • chromatin assembly Source: FlyBase
  • chromatin silencing Source: FlyBase
  • DNA repair Source: FlyBase
  • eggshell chorion gene amplification Source: FlyBase
  • histone acetylation Source: FlyBase
  • histone H3-K27 methylation Source: GOC
  • histone H3-K9 methylation Source: GOC
  • histone methylation Source: FlyBase
  • mitotic cytokinesis Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • nucleosome assembly Source: FlyBase
  • nucleosome mobilization Source: FlyBase
  • nucleosome positioning Source: FlyBase
  • positive regulation of cell proliferation Source: FlyBase
  • regulation of mitotic cell cycle Source: FlyBase
  • segment specification Source: FlyBase
  • transcription, DNA-templated Source: FlyBase

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-212300 PRC2 methylates histones and DNA
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-3214815 HDACs deacetylate histones
R-DME-3214841 PKMTs methylate histone lysines
R-DME-3214847 HATs acetylate histones
R-DME-3214858 RMTs methylate histone arginines
R-DME-8943724 Regulation of PTEN gene transcription
R-DME-8951664 Neddylation
R-DME-8953750 Transcriptional Regulation by E2F6
SignaLinkiQ24572

Names & Taxonomyi

Protein namesi
Recommended name:
Probable histone-binding protein Caf1
Alternative name(s):
Chromatin assembly factor 1 p55 subunit
Short name:
CAF-1 p55 subunit
Nucleosome-remodeling factor 55 kDa subunit
Short name:
NURF-55
dCAF-1
Gene namesi
Name:Caf1
ORF Names:CG4236
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0263979 Caf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508951 – 430Probable histone-binding protein Caf1Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11Phosphoserine1 Publication1
Modified residuei100Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24572
PRIDEiQ24572

PTM databases

iPTMnetiQ24572

Expressioni

Developmental stagei

Highest level during early embryogenesis and then decreases in larvae, pupae and adults.1 Publication

Gene expression databases

BgeeiFBgn0263979
ExpressionAtlasiQ24572 baseline and differential
GenevisibleiQ24572 DM

Interactioni

Subunit structurei

Probably binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Self associates. Associates with chromatin. Component of the CAF-1 complex, composed of Caf1, Caf1-105 and Caf1-180. Within the CAF-1 complex, Caf1-180 interacts directly with both Caf1 and Caf1-105. Component of the NuRD complex, composed of at least Caf1, Mi-2, MTA1-like and Rpd3. Within the NuRD complex, Caf1 may interact directly with Mi-2, MTA1-like and Rpd3. The NuRD complex may also associate with the methyl-DNA binding protein MBD-like via Caf1 and Mi-2. Component of the NURF complex, composed of Caf1, E(bx), Nurf-38 and Iswi. Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)1, and possibly Pho1. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. Interacts with Rbf and Rbf2. Component of the DREAM complex at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l3mbt.13 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • histone acetyltransferase binding Source: FlyBase
  • histone binding Source: FlyBase
  • histone deacetylase binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi66900, 58 interactors
DIPiDIP-23697N
IntActiQ24572, 25 interactors
MINTiQ24572
STRINGi7227.FBpp0082511

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 35Combined sources24
Beta strandi36 – 43Combined sources8
Beta strandi51 – 53Combined sources3
Beta strandi66 – 73Combined sources8
Beta strandi77 – 79Combined sources3
Beta strandi81 – 90Combined sources10
Beta strandi119 – 129Combined sources11
Beta strandi132 – 137Combined sources6
Beta strandi140 – 147Combined sources8
Beta strandi149 – 151Combined sources3
Beta strandi153 – 157Combined sources5
Helixi158 – 160Combined sources3
Beta strandi174 – 178Combined sources5
Beta strandi187 – 189Combined sources3
Beta strandi191 – 193Combined sources3
Beta strandi196 – 200Combined sources5
Beta strandi206 – 210Combined sources5
Helixi217 – 219Combined sources3
Beta strandi220 – 222Combined sources3
Beta strandi224 – 227Combined sources4
Beta strandi234 – 239Combined sources6
Beta strandi246 – 251Combined sources6
Beta strandi254 – 260Combined sources7
Beta strandi266 – 268Combined sources3
Beta strandi270 – 274Combined sources5
Beta strandi280 – 285Combined sources6
Beta strandi292 – 297Combined sources6
Beta strandi300 – 306Combined sources7
Helixi307 – 309Combined sources3
Beta strandi314 – 318Combined sources5
Beta strandi324 – 329Combined sources6
Beta strandi336 – 341Combined sources6
Beta strandi342 – 344Combined sources3
Beta strandi347 – 350Combined sources4
Helixi351 – 353Combined sources3
Helixi360 – 365Combined sources6
Beta strandi370 – 373Combined sources4
Beta strandi381 – 386Combined sources6
Beta strandi388 – 390Combined sources3
Beta strandi393 – 398Combined sources6
Beta strandi401 – 408Combined sources8
Helixi410 – 413Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XYIX-ray1.75A1-430[»]
2YB8X-ray2.30B1-418[»]
2YBAX-ray2.55A/B1-418[»]
3C99X-ray2.90A1-430[»]
3C9CX-ray3.20A1-430[»]
ProteinModelPortaliQ24572
SMRiQ24572
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ24572

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati126 – 159WD 1Add BLAST34
Repeati179 – 210WD 2Add BLAST32
Repeati229 – 260WD 3Add BLAST32
Repeati275 – 306WD 4Add BLAST32
Repeati319 – 350WD 5Add BLAST32
Repeati376 – 407WD 6Add BLAST32

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0264 Eukaryota
ENOG410XNU9 LUCA
GeneTreeiENSGT00570000079069
InParanoidiQ24572
KOiK10752
OrthoDBiEOG091G0A20
PhylomeDBiQ24572

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR022052 Histone-bd_RBBP4_N
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12265 CAF1C_H4-bd, 1 hit
PF00400 WD40, 5 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q24572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDRSDNAAE SFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT
60 70 80 90 100
AQWLPDVTKQ DGKDYSVHRL ILGTHTSDEQ NHLLIASVQL PSEDAQFDGS
110 120 130 140 150
HYDNEKGEFG GFGSVCGKIE IEIKINHEGE VNRARYMPQN ACVIATKTPS
160 170 180 190 200
SDVLVFDYTK HPSKPEPSGE CQPDLRLRGH QKEGYGLSWN PNLNGYLLSA
210 220 230 240 250
SDDHTICLWD INATPKEHRV IDAKNIFTGH TAVVEDVAWH LLHESLFGSV
260 270 280 290 300
ADDQKLMIWD TRNNNTSKPS HTVDAHTAEV NCLSFNPYSE FILATGSADK
310 320 330 340 350
TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLHVWD
360 370 380 390 400
LSKIGEEQST EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWIICSVSED
410 420 430
NIMQVWQMAE NVYNDEEPEI PASELETNTA
Length:430
Mass (Da):48,635
Last modified:November 1, 1996 - v1
Checksum:i90C5FB959F1660D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62388 mRNA Translation: AAB37257.1
AM294392 Genomic DNA Translation: CAL26322.1
AM294393 Genomic DNA Translation: CAL26323.1
AM294394 Genomic DNA Translation: CAL26324.1
AM294395 Genomic DNA Translation: CAL26325.1
AM294396 Genomic DNA Translation: CAL26330.1
AM294397 Genomic DNA Translation: CAL26335.1
AM294398 Genomic DNA Translation: CAL26337.1
AM294399 Genomic DNA Translation: CAL26338.1
AM294400 Genomic DNA Translation: CAL26339.1
AM294401 Genomic DNA Translation: CAL26340.1
AM294402 Genomic DNA Translation: CAL26341.1
AM294403 Genomic DNA Translation: CAL26342.1
AE014297 Genomic DNA Translation: AAF55146.1
AY061408 mRNA Translation: AAL28956.1
RefSeqiNP_524354.1, NM_079630.4
UniGeneiDm.1657

Genome annotation databases

EnsemblMetazoaiFBtr0083052; FBpp0082511; FBgn0263979
GeneIDi41836
KEGGidme:Dmel_CG4236

Similar proteinsi

Entry informationi

Entry nameiCAF1_DROME
AccessioniPrimary (citable) accession number: Q24572
Secondary accession number(s): A0AP04, Q9VFB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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