Q24567 (NETA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Netrin-A

Gene names

Name:	NetA
ORF Names:	CG18657

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Netrins control guidance of CNS commissural axons at the midline and peripheral motor axons to their target muscles. Ref.1 Ref.2
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	At the midline of developing CNS at the time of commissure formation and in different subsets of neurons, muscles, and epidermal patches. Ref.1 Ref.2
Sequence similarities	Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain. Contains 1 NTR domain.
Sequence caution	The sequence AAL90318.1 differs from that shown. Reason: Frameshift at positions 514 and 533.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Extracellular matrix Secreted
Domain	Laminin EGF-like domain Repeat Signal
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dendrite guidance Inferred from genetic interaction PubMed 12592406. Source: FlyBase glial cell migration Inferred from mutant phenotype PubMed 20223758. Source: FlyBase motor neuron axon guidance Inferred from mutant phenotype PubMed 16085495. Source: FlyBase regulation of photoreceptor cell axon guidance Inferred from mutant phenotype PubMed 22794263. Source: FlyBase
Cellular_component	proteinaceous extracellular matrix Inferred from sequence or structural similarity PubMed 10908592. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 726	697	Netrin-A		PRO_0000017088
Regions
	Domain	46 – 312	267	Laminin N-terminal
	Domain	313 – 368	56	Laminin EGF-like 1
	Domain	369 – 431	63	Laminin EGF-like 2
	Domain	432 – 481	50	Laminin EGF-like 3
	Domain	533 – 725	193	NTR
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	652	1	N-linked (GlcNAc...) Potential
	Glycosylation	679	1	N-linked (GlcNAc...) Potential
	Disulfide bond	313 ↔ 322		By similarity
	Disulfide bond	315 ↔ 332		By similarity
	Disulfide bond	334 ↔ 343		By similarity
	Disulfide bond	346 ↔ 366		By similarity
	Disulfide bond	369 ↔ 378		By similarity
	Disulfide bond	371 ↔ 396		By similarity
	Disulfide bond	399 ↔ 408		By similarity
	Disulfide bond	411 ↔ 429		By similarity
	Disulfide bond	432 ↔ 444		By similarity
	Disulfide bond	434 ↔ 451		By similarity
	Disulfide bond	453 ↔ 462		By similarity
	Disulfide bond	465 ↔ 479		By similarity
	Disulfide bond	533 ↔ 671		By similarity
	Disulfide bond	549 ↔ 725		By similarity
Experimental info
	Sequence conflict	22	1	S → F Ref.1
	Sequence conflict	22	1	S → F Ref.2
	Sequence conflict	501	1	E → A Ref.1
	Sequence conflict	501	1	E → A Ref.2
	Sequence conflict	514 – 516	3	GAA → APP Ref.2
	Sequence conflict	517 – 532	16	GMAAQ…EGGRE → EWPPSLSTIAPRAAGVK Ref.1
	Sequence conflict	517 – 532	16	GMAAQ…EGGRE → EWPPSLSTIAPRAAGVK Ref.2
	Sequence conflict	636	1	S → T in AAB17533. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24567 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: FCDB6372B19E30E5	FASTA	726	80,366
	10 20 30 40 50 60 MIRGILLLLL GTTRFSPIQC ISNDVYFKMF SQQAPPEDPC YNKAHEPRAC IPDFVNAAYD 70 80 90 100 110 120 APVVASSTCG SSGAQRYCEY QDHERSCHTC DMTDPLRSFP ARSLTDLNNS NNVTCWRSEP 130 140 150 160 170 180 VTGSGDNVTL TLSLGKKFEL TYVILQLCPH APRPDSMVIY KSTDHGLSWQ PFQFFSSQCR 190 200 210 220 230 240 RLFGRPARQS TGRHNEHEAR CSDVTRPLVS RIAFSTLEGR PSSRDLDSSP VLQDWVTATD 250 260 270 280 290 300 IRVVFHRLQR PDPQALLSLE AGGATDLASG KYSVPLANGP AGNNIEANLG GDVATSGSGL 310 320 330 340 350 360 HYAISDFSVG GRCKCNGHAS KCSTDASGQL NCECSHNTAG RDCERCKPFH FDRPWARATA 370 380 390 400 410 420 KEANECKECN CNKHARQCRF NMEIFRLSQG VSGGVCQNCR HSTTGRNCHQ CKEGFYRDAT 430 440 450 460 470 480 KPLTHRKVCK ACDCHPIGSS GKICNSTSGQ CPCKDGVTGL TCNRCARGYQ QSRSHIAPCI 490 500 510 520 530 540 KQPPRMINML DTQNTAPEPD EPESSPGSGG DRNGAAGMAA QSQYYRTEGG RECGKCRVST 550 560 570 580 590 600 KRLNLNKFCK RDYAIMAKVI GRDTSSEAVS REVQRRAMDP DVADYEMDQV QPGSARSPIT 610 620 630 640 650 660 GVYEFQAADY PNPNPNPRGS EMERFDLQIQ AVFKRSRPGE SSGAGNVYGM PNTTLKRGPM 670 680 690 700 710 720 TWIIPTKDLE CRCPRIRVNR SYLILGRDSE APPGYLGIGP HSIVIEWKED WYRRMKRFQR RARTCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of netrin genes in Drosophila: netrins guide CNS commissural axons and peripheral motor axons." Mitchell K.J., Doyle J.L., Serafini T., Kennedy T., Tessier-Lavigne M., Goodman C.S., Dickson B.J. Neuron 17:203-215(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[2]	"Guidance cues at the Drosophila CNS midline: identification and characterization of two Drosophila Netrin/UNC-6 homologs." Harris R., Moore-Sabatelli L., Seeger M. Neuron 17:217-228(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60316 mRNA. Translation: AAB17533.1. U63736 mRNA. Translation: AAB17547.1. AE014298 Genomic DNA. Translation: AAF48380.3. AY089580 mRNA. Translation: AAL90318.1. Frameshift.
RefSeq	NP_001245667.1. NM_001258738.1. NP_511154.3. NM_078599.4.
UniGene	Dm.2915.
3D structure databases
ProteinModelPortal	Q24567.
SMR	Q24567. Positions 66-469.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q24567. 1 interaction.
MINT	MINT-285237.
Proteomic databases
PaxDb	Q24567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073942; FBpp0073759; FBgn0015773.
GeneID	32398.
KEGG	dme:Dmel_CG18657.
Organism-specific databases
CTD	32398.
FlyBase	FBgn0015773. NetA.
Phylogenomic databases
eggNOG	NOG244580.
GeneTree	ENSGT00700000104042.
InParanoid	Q24567.
OMA	AIMAKVI.
OrthoDB	EOG470RZN.
PhylomeDB	Q24567.
Gene expression databases
Bgee	Q24567.
GermOnline	CG18657. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002049. EGF_laminin. IPR008211. Laminin_N. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR008993. TIMP-like_OB-fold. [Graphical view]
Pfam	PF00053. Laminin_EGF. 3 hits. PF00055. Laminin_N. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00180. EGF_Lam. 3 hits. SM00136. LamNT. 1 hit. [Graphical view]
SUPFAM	SSF50242. TIMP_like. 1 hit.
PROSITE	PS00022. EGF_1. 2 hits. PS01248. EGF_LAM_1. 3 hits. PS50027. EGF_LAM_2. 3 hits. PS51117. LAMININ_NTER. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32398.
NextBio	778263.

Entry information

Entry name

NETA_DROME

Accession

Primary (citable) accession number: Q24567
Secondary accession number(s): Q7JXY0, Q94528, Q9VY25

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project