Q24562 (U2AF2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Splicing factor U2AF 50 kDa subunit

Alternative name(s):
U2 auxiliary factor 50 kDa subunit
U2 snRNP auxiliary factor large subunit

Gene names

Name:	U2af50
ORF Names:	CG9998

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for the splicing of pre-mRNA. Binds to the polypyrimidine tract of introns early during spliceosome assembly By similarity.
Subunit structure	Forms a heterodimer with the U2AF small subunit.
Subcellular location	Nucleus.
Developmental stage	Present throughout development.
Sequence similarities	Belongs to the splicing factor SR family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitotic spindle organization Inferred from mutant phenotype. Source: FlyBase nuclear export Inferred from mutant phenotype. Source: FlyBase nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: FlyBase regulation of alternative nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: FlyBase
Cellular component	U2 snRNP Traceable author statement. Source: FlyBase precatalytic spliceosome Inferred from direct assay. Source: FlyBase
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro poly-pyrimidine tract binding Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Splicing factor U2AF 50 kDa subunit		PRO_0000081992
Regions
	Domain	93 – 175	83	RRM 1
	Domain	207 – 285	79	RRM 2
	Domain	318 – 408	91	RRM 3
	Compositional bias	6 – 41	36	Arg/Ser-rich (RS domain)

Sequences

	Sequence		Length	Mass (Da)
	Q24562 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 541F1544E276DFFE	FASTA	416	46,655
	10 20 30 40 50 60 MGYDDRERDR ERRRHRSRSR DRHRERSRDR RHHRNSRRKP SLYWDVPPPG FEHITPMQYK 70 80 90 100 110 120 AMQASGQIPA SVVPDTPQTA VPVVGSTITR QARRLYVGNI PFGVTEEEMM EFFNQQMHLV 130 140 150 160 170 180 GLAQAAGSPV LACQINLDKN FAFLEFRSID ETTQAMAFDG INLKGQSLKI RRPHDYQPMP 190 200 210 220 230 240 GITDTPAIKP AVVSSGVIST VVPDSPHKIF IGGLPNYLND DQVKELLLSF GKLRAFNLVK 250 260 270 280 290 300 DAATGLSKGY AFCEYVDLSI TDQSIAGLNG MQLGDKKLIV QRASVGAKNA QNAANTTQSV 310 320 330 340 350 360 MLQVPGLSNV VTSGPPTEVL CLLNMVTPDE LRDEEEYEDI LEDIKEECTK YGVVRSVEIP 370 380 390 400 410 RPIEGVEVPG CGKVFVEFNS VLDCQKAQQA LTGRKFSDRV VVTSYFDPDK YHRREF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability." Kanaar R., Roche S.E., Beall E.L., Green M.R., Rio D.C. Science 262:569-573(1993) [PubMed: 7692602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SB2040. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23404 mRNA. Translation: AAA03548.1. AE014298 Genomic DNA. Translation: AAF48596.1. AY069320 mRNA. Translation: AAL39465.1.
PIR	A48249.
RefSeq	NP_476891.1. NM_057543.3.
UniGene	Dm.5430.
3D structure databases
ProteinModelPortal	Q24562.
SMR	Q24562. Positions 92-290, 314-416.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-23323N.
IntAct	Q24562. 4 interactions.
MINT	MINT-790159.
STRING	Q24562.
Proteomic databases
PRIDE	Q24562.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074234; FBpp0074013; FBgn0005411. FBtr0307178; FBpp0298007; FBgn0005411.
GeneID	32602.
KEGG	dme:Dmel_CG9998.
NMPDR	fig|7227.3.peg.18159.
Organism-specific databases
CTD	32602.
FlyBase	FBgn0005411. U2af50.
Phylogenomic databases
eggNOG	inNOG06721.
GeneTree	EMGT00050000000048.
InParanoid	Q24562.
OMA	VEFNSIV.
OrthoDB	EOG40CFZH.
PhylomeDB	Q24562.
Gene expression databases
Bgee	Q24562.
GermOnline	CG9998. Drosophila melanogaster.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR006529. U2AF_lg. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 3 hits.
KO	K12837.
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
TIGRFAMs	TIGR01642. U2AF_lg. 1 hit.
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	779380.

Entry information

Entry name

U2AF2_DROME

Accession

Primary (citable) accession number: Q24562
Secondary accession number(s): Q9VXH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project