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Protein

Exonuclease 1

Gene

tos

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

5'->3' double-stranded DNA exonuclease which may also contain a cryptic 3'->5' double-stranded DNA exonuclease activity. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for DNA mismatch repair (MMR) (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi78Magnesium 1By similarity1
Metal bindingi150Magnesium 1By similarity1
Metal bindingi152Magnesium 1By similarity1
Metal bindingi171Magnesium 2By similarity1
Metal bindingi173Magnesium 2By similarity1
Metal bindingi226Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB
  • mismatch repair Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Endonuclease, Excision nuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA excision, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)

Names & Taxonomyi

Protein namesi
Recommended name:
Exonuclease 1 (EC:3.1.-.-)
Alternative name(s):
Exonuclease I
Protein tosca
Gene namesi
Name:tos
Synonyms:exo1
ORF Names:CG10387
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015553 tos

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540431 – 732Exonuclease 1Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei431Phosphoserine1 Publication1
Modified residuei433Phosphoserine1 Publication1
Modified residuei443Phosphothreonine1 Publication1
Modified residuei447Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24558
PRIDEiQ24558

PTM databases

iPTMnetiQ24558

Expressioni

Tissue specificityi

Specifically expressed in the female germline.1 Publication

Developmental stagei

Maternally expressed. Accumulates in the developing oocyte.1 Publication

Gene expression databases

BgeeiFBgn0015553
GenevisibleiQ24558 DM

Interactioni

Protein-protein interaction databases

BioGridi61114, 32 interactors
IntActiQ24558, 32 interactors
STRINGi7227.FBpp0080675

Structurei

3D structure databases

ProteinModelPortaliQ24558
SMRiQ24558
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 99N-domainAdd BLAST99
Regioni138 – 230I-domainAdd BLAST93

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2518 Eukaryota
COG0258 LUCA
GeneTreeiENSGT00510000047676
InParanoidiQ24558
KOiK10746
OMAiMRRCVDV
OrthoDBiEOG091G06DK
PhylomeDBiQ24558

Family and domain databases

CDDicd09908 H3TH_EXO1, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR032641 Exo1
IPR037315 EXO1_H3TH
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PTHR11081:SF8 PTHR11081:SF8, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q24558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITGLIPFV GKASSQLHLK DIRGSTVAVD TYCWLHKGVF GCAEKLARGE
60 70 80 90 100
DTDVYIQYCL KYVNMLLSYD IKPILVFDGQ HLPAKALTEK RRRDSRKQSK
110 120 130 140 150
ERAAELLRLG RIEEARSHMR RCVDVTHDMA LRLIRECRSR NVDCIVAPYE
160 170 180 190 200
ADAQMAWLNR ADVAQYIITE DSDLTLFGAK NIIFKLDLNG SGLLVEAEKL
210 220 230 240 250
HLAMGCTEEK YHFDKFRRMC ILSGCDYLDS LPGIGLAKAC KFILKTEQED
260 270 280 290 300
MRIALKKIPS YLNMRNLEVD DDYIENFMKA EATFRHMFIY NPLERRMQRL
310 320 330 340 350
CALEDYETDE RYCSNAGTLL EDSEQALHLA LGNLNPFSMK RLDSWTPEKA
360 370 380 390 400
WPTPKNVKRS KHKSIWQTNF QSENTHTPKK ENPCALFFKK VDFVGKTLNE
410 420 430 440 450
EIEANQRLEQ AKQTEAELFN MYSFKAKRRR SPSREDSVDQ ERTPPPSPVH
460 470 480 490 500
KSRHNPFAKE RTGEEANQRS PVVCENASLL RLLSPKKASP LDGEAGVKKV
510 520 530 540 550
DSLKRSIFAK EQVQIRSRFF ATQDEQTRLQ REHLRDTEND DMDEQKLSSH
560 570 580 590 600
SGHKKLRLVC KDIPGKNPIR QRCSSQISDG ETDTDTTASS LLESQDKGVP
610 620 630 640 650
SPLESQEDLN NSQPQIPTEG NTNSTTIRIK SLDLLLENSP EPTQESDRNN
660 670 680 690 700
NDAIILLSDD SCSSDQRASS TSSSSQQRQN FLPTSKRRVG LSKPSTAKKG
710 720 730
TPKSRTNGKL GAVSQNQTKL SMFGFQTKPV LK
Length:732
Mass (Da):83,181
Last modified:November 1, 1996 - v1
Checksum:iA1AF71CDFC7813AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130A → T in CAA61430 (PubMed:8812111).Curated1
Sequence conflicti701T → I in AAK93218 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89021 mRNA Translation: CAA61430.1
X89022 Genomic DNA Translation: CAA61431.1
AE014134 Genomic DNA Translation: AAF53687.1
AY051794 mRNA Translation: AAK93218.1
BT058042 mRNA Translation: ACM78484.1
RefSeqiNP_477145.1, NM_057797.4
UniGeneiDm.4197

Genome annotation databases

EnsemblMetazoaiFBtr0081131; FBpp0080675; FBgn0015553
GeneIDi35119
KEGGidme:Dmel_CG10387
UCSCiCG10387-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiEXO1_DROME
AccessioniPrimary (citable) accession number: Q24558
Secondary accession number(s): B9EQW7, Q24557, Q960X5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health