Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exonuclease 1

Gene

tos

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

5'->3' double-stranded DNA exonuclease which may also contain a cryptic 3'->5' double-stranded DNA exonuclease activity. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for DNA mismatch repair (MMR) (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Magnesium 1By similarity
Metal bindingi78 – 781Magnesium 1By similarity
Metal bindingi150 – 1501Magnesium 1By similarity
Metal bindingi152 – 1521Magnesium 1By similarity
Metal bindingi171 – 1711Magnesium 2By similarity
Metal bindingi173 – 1731Magnesium 2By similarity
Metal bindingi226 – 2261Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB
  • mismatch repair Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Excision nuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Names & Taxonomyi

Protein namesi
Recommended name:
Exonuclease 1 (EC:3.1.-.-)
Alternative name(s):
Exonuclease I
Protein tosca
Gene namesi
Name:tos
Synonyms:exo1
ORF Names:CG10387
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015553. tos.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Exonuclease 1PRO_0000154043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei431 – 4311Phosphoserine1 Publication
Modified residuei433 – 4331Phosphoserine1 Publication
Modified residuei443 – 4431Phosphothreonine1 Publication
Modified residuei447 – 4471Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24558.
PRIDEiQ24558.

PTM databases

iPTMnetiQ24558.

Expressioni

Tissue specificityi

Specifically expressed in the female germline.1 Publication

Developmental stagei

Maternally expressed. Accumulates in the developing oocyte.1 Publication

Gene expression databases

BgeeiFBgn0015553.
GenevisibleiQ24558. DM.

Interactioni

Protein-protein interaction databases

BioGridi61114. 32 interactions.
IntActiQ24558. 3 interactions.
MINTiMINT-297328.
STRINGi7227.FBpp0080675.

Structurei

3D structure databases

ProteinModelPortaliQ24558.
SMRiQ24558. Positions 2-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9999N-domainAdd
BLAST
Regioni138 – 23093I-domainAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2518. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00510000047676.
InParanoidiQ24558.
KOiK10746.
OMAiMRRCVDV.
OrthoDBiEOG091G06DK.
PhylomeDBiQ24558.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR032641. Exo1.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PTHR11081:SF8. PTHR11081:SF8. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITGLIPFV GKASSQLHLK DIRGSTVAVD TYCWLHKGVF GCAEKLARGE
60 70 80 90 100
DTDVYIQYCL KYVNMLLSYD IKPILVFDGQ HLPAKALTEK RRRDSRKQSK
110 120 130 140 150
ERAAELLRLG RIEEARSHMR RCVDVTHDMA LRLIRECRSR NVDCIVAPYE
160 170 180 190 200
ADAQMAWLNR ADVAQYIITE DSDLTLFGAK NIIFKLDLNG SGLLVEAEKL
210 220 230 240 250
HLAMGCTEEK YHFDKFRRMC ILSGCDYLDS LPGIGLAKAC KFILKTEQED
260 270 280 290 300
MRIALKKIPS YLNMRNLEVD DDYIENFMKA EATFRHMFIY NPLERRMQRL
310 320 330 340 350
CALEDYETDE RYCSNAGTLL EDSEQALHLA LGNLNPFSMK RLDSWTPEKA
360 370 380 390 400
WPTPKNVKRS KHKSIWQTNF QSENTHTPKK ENPCALFFKK VDFVGKTLNE
410 420 430 440 450
EIEANQRLEQ AKQTEAELFN MYSFKAKRRR SPSREDSVDQ ERTPPPSPVH
460 470 480 490 500
KSRHNPFAKE RTGEEANQRS PVVCENASLL RLLSPKKASP LDGEAGVKKV
510 520 530 540 550
DSLKRSIFAK EQVQIRSRFF ATQDEQTRLQ REHLRDTEND DMDEQKLSSH
560 570 580 590 600
SGHKKLRLVC KDIPGKNPIR QRCSSQISDG ETDTDTTASS LLESQDKGVP
610 620 630 640 650
SPLESQEDLN NSQPQIPTEG NTNSTTIRIK SLDLLLENSP EPTQESDRNN
660 670 680 690 700
NDAIILLSDD SCSSDQRASS TSSSSQQRQN FLPTSKRRVG LSKPSTAKKG
710 720 730
TPKSRTNGKL GAVSQNQTKL SMFGFQTKPV LK
Length:732
Mass (Da):83,181
Last modified:November 1, 1996 - v1
Checksum:iA1AF71CDFC7813AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301A → T in CAA61430 (PubMed:8812111).Curated
Sequence conflicti701 – 7011T → I in AAK93218 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89021 mRNA. Translation: CAA61430.1.
X89022 Genomic DNA. Translation: CAA61431.1.
AE014134 Genomic DNA. Translation: AAF53687.1.
AY051794 mRNA. Translation: AAK93218.1.
BT058042 mRNA. Translation: ACM78484.1.
RefSeqiNP_477145.1. NM_057797.4.
UniGeneiDm.4197.

Genome annotation databases

EnsemblMetazoaiFBtr0081131; FBpp0080675; FBgn0015553.
GeneIDi35119.
KEGGidme:Dmel_CG10387.
UCSCiCG10387-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89021 mRNA. Translation: CAA61430.1.
X89022 Genomic DNA. Translation: CAA61431.1.
AE014134 Genomic DNA. Translation: AAF53687.1.
AY051794 mRNA. Translation: AAK93218.1.
BT058042 mRNA. Translation: ACM78484.1.
RefSeqiNP_477145.1. NM_057797.4.
UniGeneiDm.4197.

3D structure databases

ProteinModelPortaliQ24558.
SMRiQ24558. Positions 2-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61114. 32 interactions.
IntActiQ24558. 3 interactions.
MINTiMINT-297328.
STRINGi7227.FBpp0080675.

PTM databases

iPTMnetiQ24558.

Proteomic databases

PaxDbiQ24558.
PRIDEiQ24558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081131; FBpp0080675; FBgn0015553.
GeneIDi35119.
KEGGidme:Dmel_CG10387.
UCSCiCG10387-RA. d. melanogaster.

Organism-specific databases

CTDi35119.
FlyBaseiFBgn0015553. tos.

Phylogenomic databases

eggNOGiKOG2518. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00510000047676.
InParanoidiQ24558.
KOiK10746.
OMAiMRRCVDV.
OrthoDBiEOG091G06DK.
PhylomeDBiQ24558.

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Miscellaneous databases

GenomeRNAii35119.
PROiQ24558.

Gene expression databases

BgeeiFBgn0015553.
GenevisibleiQ24558. DM.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR032641. Exo1.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PTHR11081:SF8. PTHR11081:SF8. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXO1_DROME
AccessioniPrimary (citable) accession number: Q24558
Secondary accession number(s): B9EQW7, Q24557, Q960X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.