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Protein

Centrosome-associated zinc finger protein CP190

Gene

Cp190

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri538 – 561C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri567 – 590C2H2-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • chromatin insulator sequence binding Source: FlyBase
  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • POZ domain binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • gene looping Source: FlyBase
  • microtubule-based process Source: FlyBase
  • nuclear axial expansion Source: FlyBase
  • positive regulation of chromatin silencing Source: FlyBase
  • regulation of cytoskeleton organization Source: UniProtKB
  • regulation of gene expression Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ24478.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosome-associated zinc finger protein CP190
Alternative name(s):
Protein enhancer of mod(mdg4)4-1
dMAP190
Gene namesi
Name:Cp190
Synonyms:E(mod)4-1
ORF Names:CG6384
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000283. Cp190.

Subcellular locationi

  • Nucleus
  • Cytoplasmcytoskeleton
  • Chromosome

  • Note: Nucleus in interphase. Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies.

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • condensed chromosome Source: FlyBase
  • cytoplasm Source: UniProtKB-KW
  • microtubule associated complex Source: FlyBase
  • nucleus Source: UniProtKB
  • polytene chromosome Source: UniProtKB
  • polytene chromosome band Source: FlyBase
  • spindle microtubule Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326291 – 1096Centrosome-associated zinc finger protein CP190Add BLAST1096

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197Phosphoserine1 Publication1
Modified residuei211Phosphoserine1 Publication1
Modified residuei229Phosphothreonine1 Publication1
Modified residuei233Phosphoserine1 Publication1
Modified residuei298Phosphoserine1 Publication1
Modified residuei319Phosphoserine1 Publication1
Modified residuei603Phosphothreonine1 Publication1
Modified residuei610Phosphoserine1 Publication1
Modified residuei708Phosphoserine1 Publication1
Modified residuei723Phosphoserine1 Publication1
Modified residuei727Phosphothreonine1 Publication1
Modified residuei745Phosphoserine1 Publication1
Modified residuei748Phosphoserine1 Publication1
Modified residuei757Phosphoserine1 Publication1
Modified residuei760Phosphoserine1 Publication1
Modified residuei817Phosphothreonine1 Publication1
Modified residuei920Phosphoserine2 Publications1
Modified residuei925Phosphoserine1 Publication1
Modified residuei927Phosphoserine1 Publication1
Modified residuei936Phosphothreonine1 Publication1
Modified residuei938Phosphoserine1 Publication1
Modified residuei1071Phosphoserine1 Publication1
Modified residuei1074Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24478.
PRIDEiQ24478.

PTM databases

iPTMnetiQ24478.

Expressioni

Tissue specificityi

Expressed in spermatids but not in mature spermatozoa. Localizes within the spermatids to a sheath of microtubules around the nucleus and to microtubules within the tail.1 Publication

Developmental stagei

Localizes to the centrosome throughout the nuclear division cycle in early syncytial embryos. Localization to the interphase nucleus is seen from nuclear cycle 9 onwards.4 Publications

Gene expression databases

BgeeiFBgn0000283.
GenevisibleiQ24478. DM.

Interactioni

Subunit structurei

Component of the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw). The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw). Interacts with Cp60 and microtubules. Interacts with inv.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTCFQ8TA445EBI-868840,EBI-466743
Ibf1Q9VHG53EBI-868840,EBI-141691
Ibf2Q9VHG63EBI-868840,EBI-157022
mod(mdg4)Q86B87-14EBI-868840,EBI-1433422
su(Hw)P089704EBI-868840,EBI-101373

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • POZ domain binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi66912. 11 interactors.
IntActiQ24478. 15 interactors.
MINTiMINT-1899376.
STRINGi7227.FBpp0082580.

Structurei

Secondary structure

11096
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 26Combined sources15
Beta strandi32 – 35Combined sources4
Beta strandi41 – 44Combined sources4
Helixi46 – 52Combined sources7
Helixi55 – 62Combined sources8
Beta strandi69 – 71Combined sources3
Helixi78 – 90Combined sources13
Helixi97 – 99Combined sources3
Helixi100 – 110Combined sources11
Helixi113 – 120Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4U77X-ray2.03A1-134[»]
5EUPX-ray2.50A1-135[»]
ProteinModelPortaliQ24478.
SMRiQ24478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 97BTBPROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 271Nuclear localizationAdd BLAST65
Regioni385 – 508Centrosomal localization and interaction with microtubulesAdd BLAST124

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri538 – 561C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri567 – 590C2H2-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
ENOG4111M3T. LUCA.
InParanoidiQ24478.
OMAiINEAHTD.
OrthoDBiEOG091G027B.
PhylomeDBiQ24478.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEVKSVKVD NWGVFFLQKL QNFFNKTDYC DLTLQFRDNS QLKVHRLVLS
60 70 80 90 100
ACTDYFNVLE QTCEIVDDAL IMPNEFQADV VVPIVNFMYT GTLEFELKMY
110 120 130 140 150
GKLLRTAKEM NMTVLLKLLE AHRRTMENVN RQQRPPSPKG IRRRTVGQPS
160 170 180 190 200
SGLPQQRVLG PSPQSRNVAT PIAQRANTQR GSTGNTMSRT SGGSNRSPYG
210 220 230 240 250
DSSNVKQEPT SPFEQLRKGY NNNKRPAQTS LLSPPSKKPS LEEVKEFAEQ
260 270 280 290 300
QRMRKQIAAE YGDNDPEYDG GMLYDDVHAG DDDDDDMPPQ PSTSKQQSPQ
310 320 330 340 350
GTQTQLEHGS TTIILKQDSP SQTPTIIVKD SSNAKLNHTK IIAEVLRQYP
360 370 380 390 400
HIVKGHKNIK LKIMPNTPAA PTEKSAPATV KPPANQSSAT TSPHKKLHVS
410 420 430 440 450
FKADKSTPLI TAQQKAASSQ QKSGTSQTTG NQGTGANPPA NTAAAQKRRI
460 470 480 490 500
DSKTMHALIA QGAENTTGPW LCLRCGVNGR PISIPSYRGF RRHLINTHKE
510 520 530 540 550
TIDPALCEHC GWRSVNNREL HFHMYMEHQT KSLLYTFAEC ALCNQSYRTK
560 570 580 590 600
GELEAHINEV HTDDNKQQCI YCNKVFEQEL QLYRHMKSYH KEQALEDGII
610 620 630 640 650
DETDEEFLGS QDEEEEAEGD EEQEPEQTGK VRILSDISLP ATSAITVQQA
660 670 680 690 700
QQEQLQEEDV EQVQQEVKFV GADGNEVELT DEQRKEILSQ LNQQQAGATA
710 720 730 740 750
GGVVMVLSEP EAEHVKQETD EKSLAGTEEE YDDSQIYSEL GAADSVESAK
760 770 780 790 800
KNIADESKES IDNLEWAENL IAESEEQSNK EPKSDKPRDD ISEKLKELTG
810 820 830 840 850
DWTEDENDDD VDDKPATAEL ASELANKDPE PTVHEEEDDI DLALQSLHKG
860 870 880 890 900
PEEATEEKAS EESVTSADDA VDAVPNINSQ PEKMDVDSEA ADEKASKAEV
910 920 930 940 950
QIKKEAELEN DQEEFIKEDS PIPHSDSVAE LREAVTASEG EDDVHLEADN
960 970 980 990 1000
IRKELLDELI AEAEKPDQEK DIVQSEENAT TEALDRSVTD EDDLVPPTQV
1010 1020 1030 1040 1050
STEQMEIDEP AAEKAAENNE DTRTADEKEA VEDKPNQTQD VTTAEKPTLE
1060 1070 1080 1090
SAKAGDEATS GEAASVDKVK SLISEWGDDD EDEDENGVSA AAKEEL
Length:1,096
Mass (Da):121,679
Last modified:April 18, 2006 - v2
Checksum:i5CD5C5492B948D39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1061G → D in CAA90324 (PubMed:8586650).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50021 mRNA. Translation: CAA90324.1.
AE014297 Genomic DNA. Translation: AAF55159.1.
AE014297 Genomic DNA. Translation: AAN13643.1.
BT010090 mRNA. Translation: AAQ22559.1.
PIRiT13802.
RefSeqiNP_524359.2. NM_079635.3.
NP_731998.1. NM_169632.2.
UniGeneiDm.2423.

Genome annotation databases

EnsemblMetazoaiFBtr0083126; FBpp0082580; FBgn0000283.
FBtr0083127; FBpp0082581; FBgn0000283.
GeneIDi41848.
KEGGidme:Dmel_CG6384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50021 mRNA. Translation: CAA90324.1.
AE014297 Genomic DNA. Translation: AAF55159.1.
AE014297 Genomic DNA. Translation: AAN13643.1.
BT010090 mRNA. Translation: AAQ22559.1.
PIRiT13802.
RefSeqiNP_524359.2. NM_079635.3.
NP_731998.1. NM_169632.2.
UniGeneiDm.2423.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4U77X-ray2.03A1-134[»]
5EUPX-ray2.50A1-135[»]
ProteinModelPortaliQ24478.
SMRiQ24478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66912. 11 interactors.
IntActiQ24478. 15 interactors.
MINTiMINT-1899376.
STRINGi7227.FBpp0082580.

PTM databases

iPTMnetiQ24478.

Proteomic databases

PaxDbiQ24478.
PRIDEiQ24478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083126; FBpp0082580; FBgn0000283.
FBtr0083127; FBpp0082581; FBgn0000283.
GeneIDi41848.
KEGGidme:Dmel_CG6384.

Organism-specific databases

CTDi41848.
FlyBaseiFBgn0000283. Cp190.

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
ENOG4111M3T. LUCA.
InParanoidiQ24478.
OMAiINEAHTD.
OrthoDBiEOG091G027B.
PhylomeDBiQ24478.

Enzyme and pathway databases

SignaLinkiQ24478.

Miscellaneous databases

GenomeRNAii41848.
PROiQ24478.

Gene expression databases

BgeeiFBgn0000283.
GenevisibleiQ24478. DM.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP190_DROME
AccessioniPrimary (citable) accession number: Q24478
Secondary accession number(s): A4V2Y3, Q9VFA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.