Reviewed, UniProtKB/Swiss-Prot Q24478 (CP190_DROME)

Names and origin

Protein names

Recommended name:
    Centrosome-associated zinc finger protein CP190
Alternative name(s):
    dMAP190
    Protein enhancer of mod(mdg4)4-1

Gene names

Name:	Cp190
Synonyms:	E(mod)4-1
ORF Names:	CG6384

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1096 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis. Ref.12 Ref.14 Ref.15
Subunit structure	Component of the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw). The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw). Interacts with Cp60 and microtubules. Ref.12 Ref.14 Ref.5 Ref.6 Ref.7 Ref.8 Ref.11
Subcellular location	Nucleus. Cytoplasm › cytoskeleton. Note: Nucleus in interphase. Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies. Ref.12 Ref.14 Ref.15 Ref.5 Ref.6 Ref.7 Ref.8 Ref.1 Ref.9 Ref.13 Ref.16
Tissue specificity	Expressed in spermatids but not in mature spermatozoa. Localizes within the spermatids to a sheath of microtubules around the nucleus and to microtubules within the tail. Ref.10
Developmental stage	Localizes to the centrosome throughout the nuclear division cycle in early syncytial embryos. Localization to the interphase nucleus is seen from nuclear cycle 9 onwards. Ref.5 Ref.6 Ref.8 Ref.1
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW nuclear axial expansion Ref.15 Inferred from mutant phenotype. Source: FlyBase regulation of cytoskeleton organization Ref.15 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Ref.1 Ref.9 Ref.12 Ref.15 Inferred from direct assay. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.1 Ref.9 Ref.12 Inferred from direct assay. Source: UniProtKB polytene chromosome Ref.1 Ref.14 Inferred from direct assay. Source: UniProtKB
Molecular function	DNA binding Ref.14 Inferred from direct assay. Source: UniProtKB chromatin binding Ref.1 Inferred from direct assay. Source: UniProtKB microtubule binding Ref.6 Ref.12 Inferred from direct assay. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1096	1096	Centrosome-associated zinc finger protein CP190		PRO_0000232629
Regions
	Domain	30 – 97	68	BTB
	Zinc finger	538 – 561	24	C2H2-type 1
	Zinc finger	567 – 590	24	C2H2-type 2
	Region	207 – 271	65	Nuclear localization
	Region	385 – 508	124	Centrosomal localization and interaction with microtubules
Amino acid modifications
	Modified residue	197	1	Phosphoserine Ref.18
	Modified residue	211	1	Phosphoserine Ref.17
	Modified residue	229	1	Phosphothreonine Ref.18
	Modified residue	233	1	Phosphoserine Ref.18
	Modified residue	298	1	Phosphoserine Ref.18
	Modified residue	319	1	Phosphoserine Ref.17
	Modified residue	603	1	Phosphothreonine Ref.18
	Modified residue	610	1	Phosphoserine Ref.18
	Modified residue	708	1	Phosphoserine Ref.18
	Modified residue	723	1	Phosphoserine Ref.18
	Modified residue	727	1	Phosphothreonine Ref.18
	Modified residue	745	1	Phosphoserine Ref.18
	Modified residue	748	1	Phosphoserine Ref.18
	Modified residue	757	1	Phosphoserine Ref.18
	Modified residue	760	1	Phosphoserine Ref.18
	Modified residue	817	1	Phosphothreonine Ref.18
	Modified residue	920	1	Phosphoserine Ref.18 Ref.17
	Modified residue	925	1	Phosphoserine Ref.17
	Modified residue	927	1	Phosphoserine Ref.18
	Modified residue	936	1	Phosphothreonine Ref.18
	Modified residue	938	1	Phosphoserine Ref.18
	Modified residue	1071	1	Phosphoserine Ref.18
	Modified residue	1074	1	Phosphoserine Ref.18
Experimental info
	Sequence conflict	1061	1	G → D in CAA90324. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24478-1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 5CD5C5492B948D39	FASTA	1,096	121,679
	10 20 30 40 50 60 MGEVKSVKVD NWGVFFLQKL QNFFNKTDYC DLTLQFRDNS QLKVHRLVLS ACTDYFNVLE 70 80 90 100 110 120 QTCEIVDDAL IMPNEFQADV VVPIVNFMYT GTLEFELKMY GKLLRTAKEM NMTVLLKLLE 130 140 150 160 170 180 AHRRTMENVN RQQRPPSPKG IRRRTVGQPS SGLPQQRVLG PSPQSRNVAT PIAQRANTQR 190 200 210 220 230 240 GSTGNTMSRT SGGSNRSPYG DSSNVKQEPT SPFEQLRKGY NNNKRPAQTS LLSPPSKKPS 250 260 270 280 290 300 LEEVKEFAEQ QRMRKQIAAE YGDNDPEYDG GMLYDDVHAG DDDDDDMPPQ PSTSKQQSPQ 310 320 330 340 350 360 GTQTQLEHGS TTIILKQDSP SQTPTIIVKD SSNAKLNHTK IIAEVLRQYP HIVKGHKNIK 370 380 390 400 410 420 LKIMPNTPAA PTEKSAPATV KPPANQSSAT TSPHKKLHVS FKADKSTPLI TAQQKAASSQ 430 440 450 460 470 480 QKSGTSQTTG NQGTGANPPA NTAAAQKRRI DSKTMHALIA QGAENTTGPW LCLRCGVNGR 490 500 510 520 530 540 PISIPSYRGF RRHLINTHKE TIDPALCEHC GWRSVNNREL HFHMYMEHQT KSLLYTFAEC 550 560 570 580 590 600 ALCNQSYRTK GELEAHINEV HTDDNKQQCI YCNKVFEQEL QLYRHMKSYH KEQALEDGII 610 620 630 640 650 660 DETDEEFLGS QDEEEEAEGD EEQEPEQTGK VRILSDISLP ATSAITVQQA QQEQLQEEDV 670 680 690 700 710 720 EQVQQEVKFV GADGNEVELT DEQRKEILSQ LNQQQAGATA GGVVMVLSEP EAEHVKQETD 730 740 750 760 770 780 EKSLAGTEEE YDDSQIYSEL GAADSVESAK KNIADESKES IDNLEWAENL IAESEEQSNK 790 800 810 820 830 840 EPKSDKPRDD ISEKLKELTG DWTEDENDDD VDDKPATAEL ASELANKDPE PTVHEEEDDI 850 860 870 880 890 900 DLALQSLHKG PEEATEEKAS EESVTSADDA VDAVPNINSQ PEKMDVDSEA ADEKASKAEV 910 920 930 940 950 960 QIKKEAELEN DQEEFIKEDS PIPHSDSVAE LREAVTASEG EDDVHLEADN IRKELLDELI 970 980 990 1000 1010 1020 AEAEKPDQEK DIVQSEENAT TEALDRSVTD EDDLVPPTQV STEQMEIDEP AAEKAAENNE 1030 1040 1050 1060 1070 1080 DTRTADEKEA VEDKPNQTQD VTTAEKPTLE SAKAGDEATS GEAASVDKVK SLISEWGDDD 1090 EDEDENGVSA AAKEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes." Whitfield W.G.F., Chaplin M.A., Oegema K., Parry H., Glover D.M. J. Cell Sci. 108:3377-3387(1995) [PubMed: 8586650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies." Kellogg D.R., Alberts B.M. Mol. Biol. Cell 3:1-11(1992) [PubMed: 1372522] [Abstract] Cited for: INTERACTION WITH CP60, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[6]	"Drosophila gamma-tubulin is part of a complex containing two previously identified centrosomal MAPs." Raff J.W., Kellogg D.R., Alberts B.M. J. Cell Biol. 121:823-835(1993) [PubMed: 8491775] [Abstract] Cited for: INTERACTION WITH CP60 AND MICROTUBULES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]	"The cell cycle-dependent localization of the CP190 centrosomal protein is determined by the coordinate action of two separable domains." Oegema K., Whitfield W.G.F., Alberts B.M. J. Cell Biol. 131:1261-1273(1995) [PubMed: 8522588] [Abstract] Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[8]	"CP60: a microtubule-associated protein that is localized to the centrosome in a cell cycle-specific manner." Kellogg D.R., Oegema K., Raff J., Schneider K., Alberts B.M. Mol. Biol. Cell 6:1673-1684(1995) [PubMed: 8590797] [Abstract] Cited for: INTERACTION WITH CP60, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[9]	"Two proteins that cycle asynchronously between centrosomes and nuclear structures: Drosophila CP60 and CP190." Oegema K., Marshall W.F., Sedat J.W., Alberts B.M. J. Cell Sci. 110:1573-1583(1997) [PubMed: 9247191] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Assembly of the zygotic centrosome in the fertilized Drosophila egg." Riparbelli M.G., Whitfield W.G.F., Dallai R., Callaini G. Mech. Dev. 65:135-144(1997) [PubMed: 9256351] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds." Moritz M., Zheng Y., Alberts B.M., Oegema K. J. Cell Biol. 142:775-786(1998) [PubMed: 9700165] [Abstract] Cited for: INTERACTION WITH CP60.
[12]	"The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell division." Butcher R.D.J., Chodagam S., Basto R., Wakefield J.G., Henderson D.S., Raff J.W., Whitfield W.G.F. J. Cell Sci. 117:1191-1199(2004) [PubMed: 14996941] [Abstract] Cited for: FUNCTION, INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[13]	"Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cells." Raynaud-Messina B., Mazzolini L., Moisand A., Cirinesi A.-M., Wright M. J. Cell Sci. 117:5497-5507(2004) [PubMed: 15479719] [Abstract] Cited for: SUBCELLULAR LOCATION.
[14]	"The centrosomal protein CP190 is a component of the gypsy chromatin insulator." Pai C.-Y., Lei E.P., Ghosh D., Corces V.G. Mol. Cell 16:737-748(2004) [PubMed: 15574329] [Abstract] Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH MOD(MDG4) AND SU(HW), SUBCELLULAR LOCATION.
[15]	"The centrosomal protein CP190 regulates myosin function during early Drosophila development." Chodagam S., Royou A., Whitfield W.G.F., Karess R., Raff J.W. Curr. Biol. 15:1308-1313(2005) [PubMed: 16051175] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[16]	"The ubiquitin ligase dTopors directs the nuclear organization of a chromatin insulator." Capelson M., Corces V.G. Mol. Cell 20:105-116(2005) [PubMed: 16209949] [Abstract] Cited for: SUBCELLULAR LOCATION.
[17]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-211; SER-319; SER-920 AND SER-925, MASS SPECTROMETRY.
[18]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197; THR-229; SER-233; SER-298; THR-603; SER-610; SER-708; SER-723; THR-727; SER-745; SER-748; SER-757; SER-760; THR-817; SER-920; SER-927; THR-936; SER-938; SER-1071 AND SER-1074, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50021 mRNA. Translation: CAA90324.1. AE014297 Genomic DNA. Translation: AAF55159.1. AE014297 Genomic DNA. Translation: AAN13643.1. BT010090 mRNA. Translation: AAQ22559.1.
PIR	T13802.
RefSeq	NP_524359.2. NP_731998.1.
UniGene	Dm.2423
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q24478. 7 interactions.
Genome annotation databases
Ensembl	FBgn0000283. Drosophila melanogaster. [Contig view]
GeneID	41848.
KEGG	dme:Dmel_CG6384.
Organism-specific databases
FlyBase	FBgn0000283. Cp190.
Phylogenomic databases
HOGENOM	Q24478.
OMA	Q24478. INTHKET.
Gene expression databases
ArrayExpress	Q24478.
GermOnline	CG6384. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 2 hits. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 4 hits. [Graphical view]
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	825881.

Entry information

Entry name

CP190_DROME

Accession

Primary (citable) accession number: Q24478
Secondary accession number(s): A4V2Y3, Q9VFA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project