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Protein

Retinoblastoma family protein

Gene

Rbf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in cell cycle regulation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In follicle cells, the complex plays a central role in the site-specific DNA replication at the chorion loci. During development, the complex represses transcription of developmentally controlled E2F target genes.2 Publications

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • RNA polymerase II regulatory region DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase
  • transcription regulatory region DNA binding Source: FlyBase

GO - Biological processi

  • DNA endoreduplication Source: FlyBase
  • eggshell chorion gene amplification Source: FlyBase
  • endomitotic cell cycle Source: FlyBase
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: FlyBase
  • maintenance of mitotic sister chromatid cohesion Source: BHF-UCL
  • mitotic G2 DNA damage checkpoint Source: FlyBase
  • negative regulation of apoptotic process Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • negative regulation of DNA replication Source: FlyBase
  • negative regulation of G1/S transition of mitotic cell cycle Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of cell death Source: FlyBase
  • regulation of cell proliferation Source: FlyBase
  • regulation of cell size Source: FlyBase
  • regulation of chromosome segregation Source: BHF-UCL
  • regulation of cohesin localization to chromatin Source: BHF-UCL
  • regulation of DNA binding Source: FlyBase
  • regulation of execution phase of apoptosis Source: FlyBase
  • response to starvation Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DME-113501. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
R-DME-1538133. G0 and Early G1.
R-DME-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69200. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
R-DME-69202. Cyclin E associated events during G1/S transition.
R-DME-69231. Cyclin D associated events in G1.
R-DME-69656. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma family protein
Gene namesi
Name:Rbf
ORF Names:CG7413
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0015799. Rbf.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • chromatin Source: FlyBase
  • Myb complex Source: FlyBase
  • nucleoplasm Source: Reactome
  • nucleus Source: FlyBase
  • Rb-E2F complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845Retinoblastoma family proteinPRO_0000167844Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561Phosphothreonine1 Publication
Modified residuei359 – 3591Phosphothreonine1 Publication
Modified residuei760 – 7601Phosphoserine1 Publication
Modified residuei771 – 7711Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation by cyclin E-dependent kinases appears to negatively regulate RBF activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24472.

PTM databases

iPTMnetiQ24472.

Expressioni

Gene expression databases

BgeeiQ24472.
GenevisibleiQ24472. DM.

Interactioni

Subunit structurei

Forms a complex with the DRTF1/E2F transcription factor through binding to a C-terminal region of E2F. This binding inhibits the E2F-mediated transactivation activity. Component of the DREAM complex at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l3mbt.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DpQ243183EBI-145741,EBI-530830
E2f1Q273682EBI-145741,EBI-108384

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi57595. 16 interactions.
DIPiDIP-34025N.
IntActiQ24472. 10 interactions.
STRINGi7227.FBpp0070141.

Structurei

3D structure databases

ProteinModelPortaliQ24472.
SMRiQ24472. Positions 192-716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni376 – 706331PocketAdd
BLAST
Regioni376 – 560185Domain AAdd
BLAST
Regioni580 – 706127Domain BAdd
BLAST

Sequence similaritiesi

Belongs to the retinoblastoma protein (RB) family.Curated

Phylogenomic databases

eggNOGiKOG1010. Eukaryota.
ENOG410XQF7. LUCA.
GeneTreeiENSGT00530000063235.
InParanoidiQ24472.
KOiK04681.
OMAiSCMRNPM.
OrthoDBiEOG7P5T04.
PhylomeDBiQ24472.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR015652. RBR.
[Graphical view]
PANTHERiPTHR13742. PTHR13742. 1 hit.
PTHR13742:SF17. PTHR13742:SF17. 1 hit.
PfamiPF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
SM01367. DUF3452. 1 hit.
SM01368. RB_A. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.

Sequencei

Sequence statusi: Complete.

Q24472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPDPQELG AEVVSGLVAT SDDRLEMINA EYTNLCRDLN MDRQTELQGY
60 70 80 90 100
ETYLEVSQRC SMEGTASHWM CCAIYTACRR TSTPTVTGQN AVVKGNCVSL
110 120 130 140 150
NNLLRCCKMS IYEFKTKIKQ WCDMANLPQE FVNEIEDLDR KFSITFMLHK
160 170 180 190 200
RFRIIMDMIF SCPPNEKKHS KYISLHGNHA HGKCSYIKLD DICWRLFLCA
210 220 230 240 250
KNQKPSNTVD LVTSYNLMIC CIDLIYNNVL AEKRTDLINP KFEGLPSNWT
260 270 280 290 300
ELDFRHNPHC ILSNFCDMTE EAKAMKATTF RQIMSSFFQA STIYGNKDTM
310 320 330 340 350
LGLLANENFE RNLKSLNISY EQYVLSVGEF DERILSAYDA GEHTALNDQS
360 370 380 390 400
LRPPVTPLTR KQDLPAQPAM AGDKFEPVRN ATNNVKQLSA FGRITEPTDF
410 420 430 440 450
VKQAGEEVIA KLLSIIEEIE QKFLAKYPST EAKSRFQLAK SFFFYLLDQI
460 470 480 490 500
LQAEIRNKPD IDLKRLLVQK VSLVIFNITL MACCVELVLE AYKTELKFPW
510 520 530 540 550
VLDCFSISAF EFQKIIEIVV RHGSHEGCLN RSLIKHLNSI EETCLERLAW
560 570 580 590 600
ARNSTVWEMI ASAQLPLPTW LMVNLDRAAG PLQIFLRKVY LLGWLRIQKL
610 620 630 640 650
CSELSLCEKT PESIWHIFEH SITHETELMK DRHLDQNIMC AIYIYIRVKR
660 670 680 690 700
MEDPKFSDIM RAYRNQPQAV NSVYREVFID INEDGEPKVK DIIHFYNHTY
710 720 730 740 750
VPLMRQFVID YLNVTPDVSG RASDLQLSPH PKERAAQPKK VTQSHSLFVS
760 770 780 790 800
QMSKNEIQQS PNQMVYSFCR SPAKDLQAMN EKVRGGKRML SFGDEPGLGT
810 820 830 840
MAETKRSKIS QVKAVMDDPE LQSAEQQTAV TTEGCVGGEG GEHET
Length:845
Mass (Da):96,826
Last modified:September 13, 2005 - v2
Checksum:iC7AF38592C14FAD2
GO

Sequence cautioni

The sequence AAK77281.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA65661.1 differs from that shown. Reason: Frameshift at position 797. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45551.1.
AL031583 Genomic DNA. Translation: CAA20903.1.
AY047549 mRNA. Translation: AAK77281.1. Different initiation.
AY089532 mRNA. Translation: AAL90270.1.
X96975 mRNA. Translation: CAA65661.1. Frameshift.
PIRiT13480.
RefSeqiNP_525036.2. NM_080297.3.
UniGeneiDm.4654.

Genome annotation databases

EnsemblMetazoaiFBtr0070146; FBpp0070141; FBgn0015799.
GeneIDi31027.
KEGGidme:Dmel_CG7413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45551.1.
AL031583 Genomic DNA. Translation: CAA20903.1.
AY047549 mRNA. Translation: AAK77281.1. Different initiation.
AY089532 mRNA. Translation: AAL90270.1.
X96975 mRNA. Translation: CAA65661.1. Frameshift.
PIRiT13480.
RefSeqiNP_525036.2. NM_080297.3.
UniGeneiDm.4654.

3D structure databases

ProteinModelPortaliQ24472.
SMRiQ24472. Positions 192-716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57595. 16 interactions.
DIPiDIP-34025N.
IntActiQ24472. 10 interactions.
STRINGi7227.FBpp0070141.

PTM databases

iPTMnetiQ24472.

Proteomic databases

PaxDbiQ24472.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070146; FBpp0070141; FBgn0015799.
GeneIDi31027.
KEGGidme:Dmel_CG7413.

Organism-specific databases

CTDi31027.
FlyBaseiFBgn0015799. Rbf.

Phylogenomic databases

eggNOGiKOG1010. Eukaryota.
ENOG410XQF7. LUCA.
GeneTreeiENSGT00530000063235.
InParanoidiQ24472.
KOiK04681.
OMAiSCMRNPM.
OrthoDBiEOG7P5T04.
PhylomeDBiQ24472.

Enzyme and pathway databases

ReactomeiR-DME-113501. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
R-DME-1538133. G0 and Early G1.
R-DME-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69200. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
R-DME-69202. Cyclin E associated events during G1/S transition.
R-DME-69231. Cyclin D associated events in G1.
R-DME-69656. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

GenomeRNAii31027.
PROiQ24472.

Gene expression databases

BgeeiQ24472.
GenevisibleiQ24472. DM.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR002720. RB_A.
IPR002719. RB_B.
IPR028309. RB_fam.
IPR024599. RB_N.
IPR015652. RBR.
[Graphical view]
PANTHERiPTHR13742. PTHR13742. 1 hit.
PTHR13742:SF17. PTHR13742:SF17. 1 hit.
PfamiPF11934. DUF3452. 1 hit.
PF01858. RB_A. 1 hit.
PF01857. RB_B. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
SM01367. DUF3452. 1 hit.
SM01368. RB_A. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oregon-R.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "RBF, a novel RB-related gene that regulates E2F activity and interacts with cyclin E in Drosophila."
    Du W., Vidal M., Xie J.-E., Dyson N.
    Genes Dev. 10:1206-1218(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-816, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  6. "Native E2F/RBF complexes contain Myb-interacting proteins and repress transcription of developmentally controlled E2F target genes."
    Korenjak M., Taylor-Harding B., Binne U.K., Satterlee J.S., Stevaux O., Aasland R., White-Cooper H., Dyson N., Brehm A.
    Cell 119:181-193(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE DREAM COMPLEX.
  7. "Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex."
    Lewis P.W., Beall E.L., Fleischer T.C., Georlette D., Link A.J., Botchan M.R.
    Genes Dev. 18:2929-2940(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE DREAM COMPLEX.
  8. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-356; THR-359; SER-760 AND SER-771, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiRBF_DROME
AccessioniPrimary (citable) accession number: Q24472
Secondary accession number(s): O77272, Q961U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 13, 2005
Last modified: June 8, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.