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Q24459

- PCL_DROME

UniProt

Q24459 - PCL_DROME

Protein

Polycomb protein Pcl

Gene

Pcl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (10 May 2004)
      Previous versions | rss
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    Functioni

    Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required. Required for the correct spatial expression of the homeotic genes of the Antennapedia and Bithorax complexes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri424 – 47249PHD-type 1CuratedPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri512 – 56049PHD-type 2CuratedPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: FlyBase
    2. DNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. chromatin modification Source: UniProtKB
    2. defense response to fungus Source: FlyBase
    3. negative regulation of transcription, DNA-templated Source: UniProtKB
    4. neurogenesis Source: FlyBase
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polycomb protein Pcl
    Alternative name(s):
    Polycomblike protein
    Gene namesi
    Name:Pcl
    ORF Names:CG5109
    OrganismiDrosophila melanogaster (Fruit fly)Imported
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0003044. Pcl.

    Subcellular locationi

    Nucleus 1 Publication. Chromosome 1 Publication
    Note: Associated with chromatin. Colocalizes with many PcG sites on polytene chromosomes. It also associates with many unique sites on polytene chromosomes.

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi517 – 5171C → S or A: Abolishes interaction with E(z). 1 Publication
    Mutagenesisi527 – 5304MLQC → QQQA: Abolishes interaction with E(z).

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10431043Polycomb protein PclPRO_0000059339Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei805 – 8051Phosphoserine1 Publication
    Modified residuei806 – 8061Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ24459.

    Expressioni

    Developmental stagei

    Ubiquitous expression in embryos.1 Publication

    Gene expression databases

    BgeeiQ24459.

    Interactioni

    Subunit structurei

    Component of a form of the Esc/E(z) complex present specifically during early embryogenesis which is composed of Caf1, esc, E(z), Su(z)12, Pcl and Rpd3. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    E(z)P4212414EBI-430086,EBI-112315
    Rpd3Q945175EBI-430086,EBI-302197

    Protein-protein interaction databases

    BioGridi62752. 19 interactions.
    IntActiQ24459. 24 interactions.
    MINTiMINT-5227221.

    Structurei

    Secondary structure

    1
    1043
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi341 – 3455
    Beta strandi356 – 3605
    Beta strandi366 – 3749
    Beta strandi379 – 3835
    Beta strandi388 – 3914
    Turni393 – 3953

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XK0NMR-A339-404[»]
    ProteinModelPortaliQ24459.
    SMRiQ24459. Positions 350-404, 424-473, 512-567.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ24459.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini349 – 40456TudorAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi237 – 25620Ser-richAdd
    BLAST
    Compositional biasi278 – 34770Pro-richAdd
    BLAST

    Domaini

    The PHD-type zinc fingers mediate the interaction with E(z).
    In contrast to vertebrate homologs (PHF1, PHF19 and MTF2), the Tudor domain does not bind H3K36me3 (PubMed:23104054 and PubMed:23142980).

    Sequence similaritiesi

    Belongs to the Polycomblike family.Curated
    Contains 2 PHD-type zinc fingers.CuratedPROSITE-ProRule annotation
    Contains 1 Tudor domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri424 – 47249PHD-type 1CuratedPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri512 – 56049PHD-type 2CuratedPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG244542.
    GeneTreeiENSGT00390000009222.
    InParanoidiQ24459.
    KOiK11485.
    OMAiDEIPIKQ.
    OrthoDBiEOG73V6JJ.
    PhylomeDBiQ24459.

    Family and domain databases

    Gene3Di3.30.40.10. 2 hits.
    InterProiIPR025894. Mtf2_C_dom.
    IPR002999. Tudor.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF14061. Mtf2_C. 1 hit.
    [Graphical view]
    SMARTiSM00249. PHD. 2 hits.
    SM00333. TUDOR. 1 hit.
    [Graphical view]
    SUPFAMiSSF57903. SSF57903. 2 hits.
    PROSITEiPS01359. ZF_PHD_1. 2 hits.
    PS50016. ZF_PHD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q24459-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNNHFHLQH DHPPQNVAHP FMQQPSTAVP SAPPATYGYL AQPAGQQPQW     50
    MTTTYQILPP SVGPATVAKR YYATTGPQTT HPTHPSTIQI TNSFAQQSTP 100
    PKQQAATSCS PFKANNIRII STAPSVYSLN KPPQEAHSTY APVQSYYLPS 150
    GGGQTAGQIN LLAASGTGKQ LQPPPLVPVT NSTSPPSTVV LDRINICINN 200
    HYTETPTSLS SSLTTAQQPS PIIPAIQHKA ILPLIDSSTA DSSSCSSSSV 250
    SSSSYSGTAT TSAAVVIVDE PDSTTTTPQT PPTTPEAMSS PGKSSPSPPL 300
    LATQSLLKGV NSMKPSFKTV EAAPPTPPTP PSPPPPPPAP PVAAPSPAVT 350
    YALQEDVFIK CNDGRFYLGT IIDQTSDQYL IRFDDQSEQW CEPDKLRKLG 400
    GGSSITAGGG GASTTESTNT SPSGPMCVAC KRSDIEDVVE ICERCGRGYH 450
    RGCTVEIVTG SGIWSCKRCA KPMKMQQPVS HKITKPAGIC RQLPYHADKL 500
    SWDEKHRVNE EQIYCYCGKP GKFDHNMLQC CKCRNWFHTQ CMQNFKKKLL 550
    RGDMFFVFCC TVCNNGIEFV RRMQIEWVDV LHIALYNLRK HQHQKYHHLL 600
    NDIWPFILEQ RHQLPICEKW RTLPETALME RLKQTLKDYS DRFVCGREFK 650
    RAPAFYALRH SGPPHIPKVF LEPHEELSDE LLEKRFKLML MPEEPDEGAN 700
    ELPKRVPKDV YEFNTDEDDP VETSEDEIPI KQIIEKAKKQ AAQKADKHDE 750
    LPLKPDLADD NANDGDPGKL PAPIPPLLDA NSSRKRKAFR LSKRYDNSRN 800
    HCDLSSDENS SSSRGTSSLD LIIPPPVNFL GRNNPFLMAT PKKASQGRSI 850
    SVGTGVGVNG IINSIFKLKG TSKEQPRMVR TIKRRLSAKD ITIGPNQEVR 900
    RRRTRRLTTA IEVISTTTIN PIPSHYLPIY AKDLQPPAPP MGKPTHGRLL 950
    RQRPQKQSPS QSRRNSTSST ATSSSSNGIG APGHSMLDLK QSVNKYFGGA 1000
    MNRIDAGEPF AIRAKRRMGN GQVQYLVEWG GDTATTAIGL LGN 1043
    Length:1,043
    Mass (Da):114,638
    Last modified:May 10, 2004 - v2
    Checksum:iBD3325D7835EEAC3
    GO

    Sequence cautioni

    The sequence AAA64457.1 differs from that shown. Reason: Frameshift at position 807.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931S → N in AAA64457. (PubMed:7956837)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35153 mRNA. Translation: AAA64457.1. Frameshift.
    AE013599 Genomic DNA. Translation: AAF57748.2.
    AY075585 mRNA. Translation: AAL68389.1.
    RefSeqiNP_001261067.1. NM_001274138.1.
    NP_476672.1. NM_057324.5.
    UniGeneiDm.4284.

    Genome annotation databases

    EnsemblMetazoaiFBtr0086735; FBpp0085914; FBgn0003044.
    FBtr0329998; FBpp0303033; FBgn0003044.
    GeneIDi37069.
    KEGGidme:Dmel_CG5109.
    UCSCiCG5109-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35153 mRNA. Translation: AAA64457.1 . Frameshift.
    AE013599 Genomic DNA. Translation: AAF57748.2 .
    AY075585 mRNA. Translation: AAL68389.1 .
    RefSeqi NP_001261067.1. NM_001274138.1.
    NP_476672.1. NM_057324.5.
    UniGenei Dm.4284.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XK0 NMR - A 339-404 [» ]
    ProteinModelPortali Q24459.
    SMRi Q24459. Positions 350-404, 424-473, 512-567.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 62752. 19 interactions.
    IntActi Q24459. 24 interactions.
    MINTi MINT-5227221.

    Proteomic databases

    PaxDbi Q24459.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0086735 ; FBpp0085914 ; FBgn0003044 .
    FBtr0329998 ; FBpp0303033 ; FBgn0003044 .
    GeneIDi 37069.
    KEGGi dme:Dmel_CG5109.
    UCSCi CG5109-RA. d. melanogaster.

    Organism-specific databases

    FlyBasei FBgn0003044. Pcl.

    Phylogenomic databases

    eggNOGi NOG244542.
    GeneTreei ENSGT00390000009222.
    InParanoidi Q24459.
    KOi K11485.
    OMAi DEIPIKQ.
    OrthoDBi EOG73V6JJ.
    PhylomeDBi Q24459.

    Miscellaneous databases

    EvolutionaryTracei Q24459.
    GenomeRNAii 37069.
    NextBioi 801791.

    Gene expression databases

    Bgeei Q24459.

    Family and domain databases

    Gene3Di 3.30.40.10. 2 hits.
    InterProi IPR025894. Mtf2_C_dom.
    IPR002999. Tudor.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF14061. Mtf2_C. 1 hit.
    [Graphical view ]
    SMARTi SM00249. PHD. 2 hits.
    SM00333. TUDOR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57903. SSF57903. 2 hits.
    PROSITEi PS01359. ZF_PHD_1. 2 hits.
    PS50016. ZF_PHD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterisation of the Polycomblike gene of Drosophila melanogaster, a trans-acting negative regulator of homeotic gene expression."
      Lonie A., D'andrea R., Paro R., Saint R.
      Development 120:2629-2636(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Tissue: Embryo1 Publication.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley1 Publication.
      Tissue: Embryo1 Publication.
    5. "Polycomblike PHD fingers mediate conserved interaction with enhancer of zeste protein."
      O'Connell S., Wang L., Robert S., Jones C.A., Saint R., Jones R.S.
      J. Biol. Chem. 276:43065-43073(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH E(Z), MUTAGENESIS OF CYS-517 AND 527-MET--CYS-530.
    6. "A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3."
      Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J.
      Mol. Cell. Biol. 23:3352-3362(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z); SU(Z)12 AND RPD3.
    7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-805 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.
    8. Cited for: DOMAIN.
    9. Cited for: DOMAIN.

    Entry informationi

    Entry nameiPCL_DROME
    AccessioniPrimary (citable) accession number: Q24459
    Secondary accession number(s): Q8T8P9, Q9V8C2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3