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Q24395 (MTK_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length52 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potent antifungal and antibacterial activity against Gram-positive bacteria.

Subcellular location

Secreted.

Tissue specificity

Highest expression in fat body. Maybe blood cells participate in the production of the peptide. In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks. Ref.7

Developmental stage

Expressed rapidly and strongly at all stages.

Polymorphism

There are 2 allelic forms (A1 and A2) varying in two positions. The isoform shownhere is A1.

Mass spectrometry

Molecular mass is 3045.4 Da from positions 27 - 52. Determined by MALDI. Variant Arg-29. Ref.7

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 262
PRO_0000004983
Peptide27 – 5226Metchnikowin Ref.1
PRO_0000004984

Natural variations

Natural variant191A → T in strain: 2CPA51. Ref.3
Natural variant291H → R in strain: 2CPA51. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q24395 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0B1E2112BAE03129

FASTA525,654
        10         20         30         40         50 
MQLNLGAIFL ALLGVMATAT SVLAEPHRHQ GPIFDTRPSP FNPNQPRPGP IY 

« Hide

References

« Hide 'large scale' references
[1]"Metchnikowin, a novel immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties."
Levashina E.A., Ohresser S., Bulet P., Reichhart J.-M., Hetru C., Hoffmann J.A.
Eur. J. Biochem. 233:694-700(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 27-52.
Strain: Oregon-R.
Tissue: Abdomen and Thorax.
[2]"Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin."
Levashina E.A., Ohresser S., Lemaitre B., Imler J.-L.
J. Mol. Biol. 278:515-527(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Oregon-R.
[3]"Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster."
Lazzaro B.P., Clark A.G.
Mol. Biol. Evol. 20:914-923(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, VARIANTS THR-19 AND ARG-29.
Strain: 2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14, 2CPA43, 2CPA46, 2CPA51 and 2CPA7.
[4]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[5]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Head.
[7]"Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91060 mRNA. Translation: CAA62511.1.
X91061 mRNA. Translation: CAA62512.1.
AF030959 Genomic DNA. Translation: AAC64659.1.
AY224619 Genomic DNA. Translation: AAO72478.1.
AY224620 Genomic DNA. Translation: AAO72479.1.
AY224621 Genomic DNA. Translation: AAO72480.1.
AY224622 Genomic DNA. Translation: AAO72481.1.
AY224623 Genomic DNA. Translation: AAO72482.1.
AY224624 Genomic DNA. Translation: AAO72483.1.
AY224625 Genomic DNA. Translation: AAO72484.1.
AY224626 Genomic DNA. Translation: AAO72485.1.
AY224627 Genomic DNA. Translation: AAO72486.1.
AY224628 Genomic DNA. Translation: AAO72487.1.
AY224629 Genomic DNA. Translation: AAO72488.1.
AY224630 Genomic DNA. Translation: AAO72489.1.
AE013599 Genomic DNA. Translation: AAF58139.1.
AY075536 mRNA. Translation: AAL68343.1.
PIRS63981.
RefSeqNP_523752.1. NM_079028.3.
UniGeneDm.19934.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EZSX-ray1.90B46-52[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-23804N.
MINTMINT-344578.

Proteomic databases

PRIDEQ24395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0087386; FBpp0086518; FBgn0014865.
GeneID36708.
KEGGdme:Dmel_CG8175.

Organism-specific databases

CTD36708.
FlyBaseFBgn0014865. Mtk.

Phylogenomic databases

eggNOGNOG246900.
InParanoidQ24395.
OMANQPRPGP.
OrthoDBEOG7FV3TH.
PhylomeDBQ24395.

Family and domain databases

InterProIPR012513. Antimicrobial10.
[Graphical view]
PfamPF08105. Antimicrobial10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi36708.
NextBio799983.

Entry information

Entry nameMTK_DROME
AccessionPrimary (citable) accession number: Q24395
Secondary accession number(s): Q24396, Q86BV8, Q9V7B9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase