Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q24395

- MTK_DROME

UniProt

Q24395 - MTK_DROME

Protein

Metchnikowin

Gene

Mtk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Potent antifungal and antibacterial activity against Gram-positive bacteria.

    GO - Biological processi

    1. antibacterial humoral response Source: FlyBase
    2. antifungal humoral response Source: FlyBase
    3. defense response Source: FlyBase
    4. defense response to fungus Source: FlyBase
    5. defense response to Gram-negative bacterium Source: FlyBase
    6. defense response to Gram-positive bacterium Source: FlyBase
    7. innate immune response Source: UniProtKB-KW
    8. killing of cells of other organism Source: UniProtKB-KW
    9. response to bacterium Source: FlyBase

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Fungicide

    Keywords - Biological processi

    Immunity, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metchnikowin
    Gene namesi
    Name:Mtk
    ORF Names:CG8175
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0014865. Mtk.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: FlyBase

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 2621 PublicationPRO_0000004983
    Peptidei27 – 5226MetchnikowinPRO_0000004984Add
    BLAST

    Proteomic databases

    PRIDEiQ24395.

    Expressioni

    Tissue specificityi

    Highest expression in fat body. Maybe blood cells participate in the production of the peptide. In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks.1 Publication

    Developmental stagei

    Expressed rapidly and strongly at all stages.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-23804N.
    MINTiMINT-344578.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EZSX-ray1.90B46-52[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG246900.
    InParanoidiQ24395.
    OMAiPALMEIT.
    OrthoDBiEOG7FV3TH.
    PhylomeDBiQ24395.

    Family and domain databases

    InterProiIPR012513. Antimicrobial10.
    [Graphical view]
    PfamiPF08105. Antimicrobial10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q24395-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLNLGAIFL ALLGVMATAT SVLAEPHRHQ GPIFDTRPSP FNPNQPRPGP   50
    IY 52
    Length:52
    Mass (Da):5,654
    Last modified:November 1, 1997 - v1
    Checksum:i0B1E2112BAE03129
    GO

    Mass spectrometryi

    Molecular mass is 3045.4 Da from positions 27 - 52. Determined by MALDI. Variant Arg-29.1 Publication

    Polymorphismi

    There are 2 allelic forms (A1 and A2) varying in two positions. The isoform shown here is A1.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti19 – 191A → T in strain: 2CPA51. 1 Publication
    Natural varianti29 – 291H → R in strain: 2CPA51. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91060 mRNA. Translation: CAA62511.1.
    X91061 mRNA. Translation: CAA62512.1.
    AF030959 Genomic DNA. Translation: AAC64659.1.
    AY224619 Genomic DNA. Translation: AAO72478.1.
    AY224620 Genomic DNA. Translation: AAO72479.1.
    AY224621 Genomic DNA. Translation: AAO72480.1.
    AY224622 Genomic DNA. Translation: AAO72481.1.
    AY224623 Genomic DNA. Translation: AAO72482.1.
    AY224624 Genomic DNA. Translation: AAO72483.1.
    AY224625 Genomic DNA. Translation: AAO72484.1.
    AY224626 Genomic DNA. Translation: AAO72485.1.
    AY224627 Genomic DNA. Translation: AAO72486.1.
    AY224628 Genomic DNA. Translation: AAO72487.1.
    AY224629 Genomic DNA. Translation: AAO72488.1.
    AY224630 Genomic DNA. Translation: AAO72489.1.
    AE013599 Genomic DNA. Translation: AAF58139.1.
    AY075536 mRNA. Translation: AAL68343.1.
    PIRiS63981.
    RefSeqiNP_523752.1. NM_079028.3.
    UniGeneiDm.19934.

    Genome annotation databases

    EnsemblMetazoaiFBtr0087386; FBpp0086518; FBgn0014865.
    GeneIDi36708.
    KEGGidme:Dmel_CG8175.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91060 mRNA. Translation: CAA62511.1 .
    X91061 mRNA. Translation: CAA62512.1 .
    AF030959 Genomic DNA. Translation: AAC64659.1 .
    AY224619 Genomic DNA. Translation: AAO72478.1 .
    AY224620 Genomic DNA. Translation: AAO72479.1 .
    AY224621 Genomic DNA. Translation: AAO72480.1 .
    AY224622 Genomic DNA. Translation: AAO72481.1 .
    AY224623 Genomic DNA. Translation: AAO72482.1 .
    AY224624 Genomic DNA. Translation: AAO72483.1 .
    AY224625 Genomic DNA. Translation: AAO72484.1 .
    AY224626 Genomic DNA. Translation: AAO72485.1 .
    AY224627 Genomic DNA. Translation: AAO72486.1 .
    AY224628 Genomic DNA. Translation: AAO72487.1 .
    AY224629 Genomic DNA. Translation: AAO72488.1 .
    AY224630 Genomic DNA. Translation: AAO72489.1 .
    AE013599 Genomic DNA. Translation: AAF58139.1 .
    AY075536 mRNA. Translation: AAL68343.1 .
    PIRi S63981.
    RefSeqi NP_523752.1. NM_079028.3.
    UniGenei Dm.19934.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EZS X-ray 1.90 B 46-52 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-23804N.
    MINTi MINT-344578.

    Proteomic databases

    PRIDEi Q24395.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0087386 ; FBpp0086518 ; FBgn0014865 .
    GeneIDi 36708.
    KEGGi dme:Dmel_CG8175.

    Organism-specific databases

    CTDi 36708.
    FlyBasei FBgn0014865. Mtk.

    Phylogenomic databases

    eggNOGi NOG246900.
    InParanoidi Q24395.
    OMAi PALMEIT.
    OrthoDBi EOG7FV3TH.
    PhylomeDBi Q24395.

    Miscellaneous databases

    GenomeRNAii 36708.
    NextBioi 799983.

    Family and domain databases

    InterProi IPR012513. Antimicrobial10.
    [Graphical view ]
    Pfami PF08105. Antimicrobial10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metchnikowin, a novel immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties."
      Levashina E.A., Ohresser S., Bulet P., Reichhart J.-M., Hetru C., Hoffmann J.A.
      Eur. J. Biochem. 233:694-700(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 27-52.
      Strain: Oregon-R.
      Tissue: Abdomen and Thorax.
    2. "Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin."
      Levashina E.A., Ohresser S., Lemaitre B., Imler J.-L.
      J. Mol. Biol. 278:515-527(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    3. "Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster."
      Lazzaro B.P., Clark A.G.
      Mol. Biol. Evol. 20:914-923(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, VARIANTS THR-19 AND ARG-29.
      Strain: 2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14, 2CPA43, 2CPA46, 2CPA51 and 2CPA7.
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.
    7. "Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
      Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
      Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMTK_DROME
    AccessioniPrimary (citable) accession number: Q24395
    Secondary accession number(s): Q24396, Q86BV8, Q9V7B9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3