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Q24395

- MTK_DROME

UniProt

Q24395 - MTK_DROME

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Protein
Metchnikowin
Gene
Mtk, CG8175
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Potent antifungal and antibacterial activity against Gram-positive bacteria.

GO - Biological processi

  1. antibacterial humoral response Source: FlyBase
  2. antifungal humoral response Source: FlyBase
  3. defense response Source: FlyBase
  4. defense response to Gram-negative bacterium Source: FlyBase
  5. defense response to Gram-positive bacterium Source: FlyBase
  6. defense response to fungus Source: FlyBase
  7. innate immune response Source: UniProtKB-KW
  8. killing of cells of other organism Source: UniProtKB-KW
  9. response to bacterium Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Metchnikowin
Gene namesi
Name:Mtk
ORF Names:CG8175
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0014865. Mtk.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Propeptidei25 – 262
PRO_0000004983
Peptidei27 – 5226Metchnikowin1 Publication
PRO_0000004984Add
BLAST

Proteomic databases

PRIDEiQ24395.

Expressioni

Tissue specificityi

Highest expression in fat body. Maybe blood cells participate in the production of the peptide. In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks.1 Publication

Developmental stagei

Expressed rapidly and strongly at all stages.

Interactioni

Protein-protein interaction databases

DIPiDIP-23804N.
MINTiMINT-344578.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EZSX-ray1.90B46-52[»]

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG246900.
InParanoidiQ24395.
OMAiPALMEIT.
OrthoDBiEOG7FV3TH.
PhylomeDBiQ24395.

Family and domain databases

InterProiIPR012513. Antimicrobial10.
[Graphical view]
PfamiPF08105. Antimicrobial10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q24395-1 [UniParc]FASTAAdd to Basket

« Hide

MQLNLGAIFL ALLGVMATAT SVLAEPHRHQ GPIFDTRPSP FNPNQPRPGP   50
IY 52
Length:52
Mass (Da):5,654
Last modified:November 1, 1997 - v1
Checksum:i0B1E2112BAE03129
GO

Mass spectrometryi

Molecular mass is 3045.4 Da from positions 27 - 52. Determined by MALDI. Variant Arg-29.1 Publication

Polymorphismi

There are 2 allelic forms (A1 and A2) varying in two positions. The isoform shown here is A1.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191A → T in strain: 2CPA51. 1 Publication
Natural varianti29 – 291H → R in strain: 2CPA51. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91060 mRNA. Translation: CAA62511.1.
X91061 mRNA. Translation: CAA62512.1.
AF030959 Genomic DNA. Translation: AAC64659.1.
AY224619 Genomic DNA. Translation: AAO72478.1.
AY224620 Genomic DNA. Translation: AAO72479.1.
AY224621 Genomic DNA. Translation: AAO72480.1.
AY224622 Genomic DNA. Translation: AAO72481.1.
AY224623 Genomic DNA. Translation: AAO72482.1.
AY224624 Genomic DNA. Translation: AAO72483.1.
AY224625 Genomic DNA. Translation: AAO72484.1.
AY224626 Genomic DNA. Translation: AAO72485.1.
AY224627 Genomic DNA. Translation: AAO72486.1.
AY224628 Genomic DNA. Translation: AAO72487.1.
AY224629 Genomic DNA. Translation: AAO72488.1.
AY224630 Genomic DNA. Translation: AAO72489.1.
AE013599 Genomic DNA. Translation: AAF58139.1.
AY075536 mRNA. Translation: AAL68343.1.
PIRiS63981.
RefSeqiNP_523752.1. NM_079028.3.
UniGeneiDm.19934.

Genome annotation databases

EnsemblMetazoaiFBtr0087386; FBpp0086518; FBgn0014865.
GeneIDi36708.
KEGGidme:Dmel_CG8175.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91060 mRNA. Translation: CAA62511.1 .
X91061 mRNA. Translation: CAA62512.1 .
AF030959 Genomic DNA. Translation: AAC64659.1 .
AY224619 Genomic DNA. Translation: AAO72478.1 .
AY224620 Genomic DNA. Translation: AAO72479.1 .
AY224621 Genomic DNA. Translation: AAO72480.1 .
AY224622 Genomic DNA. Translation: AAO72481.1 .
AY224623 Genomic DNA. Translation: AAO72482.1 .
AY224624 Genomic DNA. Translation: AAO72483.1 .
AY224625 Genomic DNA. Translation: AAO72484.1 .
AY224626 Genomic DNA. Translation: AAO72485.1 .
AY224627 Genomic DNA. Translation: AAO72486.1 .
AY224628 Genomic DNA. Translation: AAO72487.1 .
AY224629 Genomic DNA. Translation: AAO72488.1 .
AY224630 Genomic DNA. Translation: AAO72489.1 .
AE013599 Genomic DNA. Translation: AAF58139.1 .
AY075536 mRNA. Translation: AAL68343.1 .
PIRi S63981.
RefSeqi NP_523752.1. NM_079028.3.
UniGenei Dm.19934.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EZS X-ray 1.90 B 46-52 [» ]
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-23804N.
MINTi MINT-344578.

Proteomic databases

PRIDEi Q24395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0087386 ; FBpp0086518 ; FBgn0014865 .
GeneIDi 36708.
KEGGi dme:Dmel_CG8175.

Organism-specific databases

CTDi 36708.
FlyBasei FBgn0014865. Mtk.

Phylogenomic databases

eggNOGi NOG246900.
InParanoidi Q24395.
OMAi PALMEIT.
OrthoDBi EOG7FV3TH.
PhylomeDBi Q24395.

Miscellaneous databases

GenomeRNAii 36708.
NextBioi 799983.

Family and domain databases

InterProi IPR012513. Antimicrobial10.
[Graphical view ]
Pfami PF08105. Antimicrobial10. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Metchnikowin, a novel immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties."
    Levashina E.A., Ohresser S., Bulet P., Reichhart J.-M., Hetru C., Hoffmann J.A.
    Eur. J. Biochem. 233:694-700(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 27-52.
    Strain: Oregon-R.
    Tissue: Abdomen and Thorax.
  2. "Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin."
    Levashina E.A., Ohresser S., Lemaitre B., Imler J.-L.
    J. Mol. Biol. 278:515-527(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  3. "Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster."
    Lazzaro B.P., Clark A.G.
    Mol. Biol. Evol. 20:914-923(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, VARIANTS THR-19 AND ARG-29.
    Strain: 2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14, 2CPA43, 2CPA46, 2CPA51 and 2CPA7.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  7. "Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
    Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
    Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMTK_DROME
AccessioniPrimary (citable) accession number: Q24395
Secondary accession number(s): Q24396, Q86BV8, Q9V7B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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