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Protein

Chromatin-remodeling complex ATPase chain Iswi

Gene

Iswi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Energy-transducing component of the chromatin-remodeling complexes NURF (nucleosome-remodeling factor), ACF (ATP-utilizing chromatin assembly and remodeling factor), and CHRAC (chromatin accessibility complex). NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi153 – 160ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: FlyBase
  • DNA binding Source: InterPro
  • DNA-dependent ATPase activity Source: FlyBase
  • DNA helicase activity Source: FlyBase
  • nucleosome binding Source: InterPro
  • nucleosome-dependent ATPase activity Source: FlyBase
  • nucleotide binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: FlyBase
  • chromatin assembly or disassembly Source: FlyBase
  • chromatin modification Source: UniProtKB-KW
  • chromatin organization Source: FlyBase
  • chromatin remodeling Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • ecdysone receptor-mediated signaling pathway Source: FlyBase
  • muscle organ development Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • nuclear speck organization Source: FlyBase
  • nucleosome assembly Source: FlyBase
  • nucleosome mobilization Source: FlyBase
  • nucleosome positioning Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of circadian rhythm Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • sensory perception of pain Source: FlyBase
  • spermatogenesis Source: FlyBase
  • sperm chromatin condensation Source: FlyBase
  • sperm chromatin decondensation Source: FlyBase
  • transcription, DNA-templated Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin-remodeling complex ATPase chain Iswi (EC:3.6.4.-)
Alternative name(s):
CHRAC 140 kDa subunit
Nucleosome-remodeling factor 140 kDa subunit
Short name:
NURF-140
Protein imitation swi
Gene namesi
Name:Iswi
ORF Names:CG8625
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011604. Iswi.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • ACF complex Source: FlyBase
  • CHRAC Source: FlyBase
  • ISWI-type complex Source: FlyBase
  • nucleus Source: FlyBase
  • NURF complex Source: FlyBase
  • polytene chromosome Source: FlyBase
  • RSF complex Source: FlyBase
  • transcription factor complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743331 – 1027Chromatin-remodeling complex ATPase chain IswiAdd BLAST1027

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24368.
PRIDEiQ24368.

PTM databases

iPTMnetiQ24368.

Expressioni

Developmental stagei

Present throughout embryonic, larval and pupal development and in female adults. Present at low levels in adult males (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0011604.
GenevisibleiQ24368. DM.

Interactioni

Subunit structurei

Component of the NURF complex composed of Caf1, E(bx), Nurf-38 and Iswi.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
armP188242EBI-367628,EBI-216128

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi62163. 14 interactors.
DIPiDIP-24067N.
IntActiQ24368. 6 interactors.
MINTiMINT-252539.
STRINGi7227.FBpp0086954.

Structurei

Secondary structure

11027
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi698 – 705Combined sources8
Helixi727 – 729Combined sources3
Helixi733 – 749Combined sources17
Helixi766 – 776Combined sources11
Helixi783 – 792Combined sources10
Helixi802 – 815Combined sources14
Helixi820 – 823Combined sources4
Helixi832 – 845Combined sources14
Helixi846 – 848Combined sources3
Helixi852 – 881Combined sources30
Helixi886 – 889Combined sources4
Helixi904 – 917Combined sources14
Helixi924 – 934Combined sources11
Helixi936 – 938Combined sources3
Helixi942 – 945Combined sources4
Helixi949 – 974Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OFCX-ray1.90X691-991[»]
ProteinModelPortaliQ24368.
SMRiQ24368.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ24368.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 305Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini435 – 586Helicase C-terminalPROSITE-ProRule annotationAdd BLAST152
Domaini795 – 847SANT 1PROSITE-ProRule annotationAdd BLAST53
Domaini898 – 962SANT 2PROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi256 – 259DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi978 – 981Poly-Lys4
Compositional biasi1023 – 1027Poly-Lys5

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0385. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00860000133761.
InParanoidiQ24368.
KOiK11654.
OMAiVCTRFEE.
OrthoDBiEOG091G01GJ.
PhylomeDBiQ24368.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR020838. DBINO.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF13892. DBINO. 1 hit.
PF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTDTAAVE ATEENSNETT SDAATSSSGE KEAEFDNKIE ADRSRRFDFL
60 70 80 90 100
LKQTEIFTHF MTNSAKSPTK PKGRPKKIKD KDKEKDVADH RHRKTEQEED
110 120 130 140 150
EELLAEDSAT KEIFRFDASP AYIKSGEMRD YQIRGLNWMI SLYENGINGI
160 170 180 190 200
LADEMGLGKT LQTISLLGYL KHFKNQAGPH IVIVPKSTLQ NWVNEFKKWC
210 220 230 240 250
PSLRAVCLIG DQDTRNTFIR DVLMPGEWDV CVTSYEMCIR EKSVFKKFNW
260 270 280 290 300
RYLVIDEAHR IKNEKSKLSE ILREFKTANR LLITGTPLQN NLHELWALLN
310 320 330 340 350
FLLPDVFNSS EDFDEWFNTN TCLGDDALIT RLHAVLKPFL LRRLKAEVEK
360 370 380 390 400
RLKPKKEMKI FVGLSKMQRD WYTKVLLKDI DVVNGAGKVE KMRLQNILMQ
410 420 430 440 450
LRKCTNHPYL FDGAEPGPPY TTDTHLVYNS GKMAILDKLL PKLQEQGSRV
460 470 480 490 500
LIFSQMTRML DILEDYCHWR NYNYCRLDGQ TPHEDRNRQI QEFNMDNSAK
510 520 530 540 550
FLFMLSTRAG GLGINLATAD VVIIYDSDWN PQMDLQAMDR AHRIGQKKQV
560 570 580 590 600
RVFRLITEST VEEKIVERAE VKLRLDKMVI QGGRLVDNRS NQLNKDEMLN
610 620 630 640 650
IIRFGANQVF SSKETDITDE DIDVILERGE AKTAEQKAAL DSLGESSLRT
660 670 680 690 700
FTMDTNGEAG TSSVYQFEGE DWREKQKLNA LGNWIEPPKR ERKANYAVDA
710 720 730 740 750
YFREALRVSE PKAPKAPRPP KQPIVQDFQF FPPRLFELLD QEIYYFRKTV
760 770 780 790 800
GYKVPKNTEL GSDATKVQRE EQRKIDEAEP LTEEEIQEKE NLLSQGFTAW
810 820 830 840 850
TKRDFNQFIK ANEKYGRDDI DNIAKDVEGK TPEEVIEYNA VFWERCTELQ
860 870 880 890 900
DIERIMGQIE RGEGKIQRRL SIKKALDQKM SRYRAPFHQL RLQYGNNKGK
910 920 930 940 950
NYTEIEDRFL VCMLHKLGFD KENVYEELRA AIRASPQFRF DWFIKSRTAL
960 970 980 990 1000
ELQRRCNTLI TLIERENIEL EEKERAEKKK KAPKGSVSAG SGSASSNTPA
1010 1020
PAPQPKASQK RKSEVVATSS NSKKKKK
Length:1,027
Mass (Da):118,873
Last modified:November 1, 1996 - v1
Checksum:i008FC81AE15E071F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti645E → D in AAM11261 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27127 mRNA. Translation: AAA19868.1.
AE013599 Genomic DNA. Translation: AAF58479.1.
AE013599 Genomic DNA. Translation: AAM68638.1.
AY094908 mRNA. Translation: AAM11261.1.
BT044562 mRNA. Translation: ACI15757.1.
PIRiA56533.
RefSeqiNP_523719.1. NM_078995.2.
NP_725203.1. NM_165930.2.
NP_725204.1. NM_165931.2.
UniGeneiDm.2581.

Genome annotation databases

EnsemblMetazoaiFBtr0087841; FBpp0086954; FBgn0011604.
FBtr0087842; FBpp0086955; FBgn0011604.
FBtr0087843; FBpp0086956; FBgn0011604.
GeneIDi36390.
KEGGidme:Dmel_CG8625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27127 mRNA. Translation: AAA19868.1.
AE013599 Genomic DNA. Translation: AAF58479.1.
AE013599 Genomic DNA. Translation: AAM68638.1.
AY094908 mRNA. Translation: AAM11261.1.
BT044562 mRNA. Translation: ACI15757.1.
PIRiA56533.
RefSeqiNP_523719.1. NM_078995.2.
NP_725203.1. NM_165930.2.
NP_725204.1. NM_165931.2.
UniGeneiDm.2581.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OFCX-ray1.90X691-991[»]
ProteinModelPortaliQ24368.
SMRiQ24368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62163. 14 interactors.
DIPiDIP-24067N.
IntActiQ24368. 6 interactors.
MINTiMINT-252539.
STRINGi7227.FBpp0086954.

PTM databases

iPTMnetiQ24368.

Proteomic databases

PaxDbiQ24368.
PRIDEiQ24368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087841; FBpp0086954; FBgn0011604.
FBtr0087842; FBpp0086955; FBgn0011604.
FBtr0087843; FBpp0086956; FBgn0011604.
GeneIDi36390.
KEGGidme:Dmel_CG8625.

Organism-specific databases

CTDi36390.
FlyBaseiFBgn0011604. Iswi.

Phylogenomic databases

eggNOGiKOG0385. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00860000133761.
InParanoidiQ24368.
KOiK11654.
OMAiVCTRFEE.
OrthoDBiEOG091G01GJ.
PhylomeDBiQ24368.

Miscellaneous databases

EvolutionaryTraceiQ24368.
GenomeRNAii36390.
PROiQ24368.

Gene expression databases

BgeeiFBgn0011604.
GenevisibleiQ24368. DM.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR020838. DBINO.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF13892. DBINO. 1 hit.
PF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISWI_DROME
AccessioniPrimary (citable) accession number: Q24368
Secondary accession number(s): A4UZF0
, B5X0J3, Q0E9A4, Q8SX14, Q9V6E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.