Q24325 (TAF2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 2

Alternative name(s):
Transcription initiation factor TFIID 150 kDa subunit
Short name=TAF(II)150
Short name=TAFII-150
Short name=TAFII150

Gene names

Name:	Taf2
Synonyms:	TAF150
ORF Names:	CG6711

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. An essential subunit binds to core promoter DNA. Ref.1
Subunit structure	Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (TAFs). Interacts with Tbp, Taf1, Taf11 and Taf12. Ref.1 Ref.5
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TAF2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred from direct assay. Source: BHF-UCL positive regulation of transcription initiation from RNA polymerase II promoter Inferred from direct assay. Source: BHF-UCL
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1221	1221	Transcription initiation factor TFIID subunit 2		PRO_0000118866
Regions
	Region	845 – 1213	369	Binds to Tbp and Taf1
	Region	1138 – 1183	46	Highly charged
Amino acid modifications
	Modified residue	1135	1	Phosphoserine Ref.6
	Modified residue	1136	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	53	1	R → S in CAA55830. Ref.1
	Sequence conflict	88	1	H → P in CAA55830. Ref.1
	Sequence conflict	1175 – 1221	47	DKDKE…PMNLN → ERKDKDKRDPHISRLQAARQ PLRTLSARRTVATAIACRP AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24325 [UniParc]. Last modified January 27, 2003. Version 2. Checksum: C2DC066826B1AF6E	FASTA	1,221	139,499
	10 20 30 40 50 60 METQPEVPEV PLRPFKLAHQ VVSLTGISFE RRSIIGVVEL TIVPNSENLR LIRLNAKQLR 70 80 90 100 110 120 IYSVVLNDVC QADFTYFDPF QNICYKEHKS RALEVYSKHH LTAAQYTDPD VNNGELLIQV 130 140 150 160 170 180 PPEGYSMIQE GQGLRIRIEF SLENPKCGVH FVIPPASTDE ETQMNSSHMF TNCYENSSRL 190 200 210 220 230 240 WFPCVDSFAD PCTWRLEFTV DKNMTAVSCG ELLEVIMTPD LRKKTFHYSV STPVCAPNIA 250 260 270 280 290 300 LAVGQFEIYV DPHMHEVTHF CLPGLLPLLK NTVRYLHEAF EFYEETLSTR YPFSCYKQVF 310 320 330 340 350 360 VDELDTDISA YATMSIASVN LLHSIAIIDQ TYISRTFMSR AVAEQFFGCF ITSHHWSDTW 370 380 390 400 410 420 LAKGIAEYLC GLYSRKCFGN NEYRAWVQSE LARVVRYEEQ YGGIILDCSQ PPAPLPVSGT 430 440 450 460 470 480 NQSAASSKQQ EIVHYFPIKS LHTVSPKYVE AMRRKAHFVI RMLENRIGQE LLIQVFNKQL 490 500 510 520 530 540 ALASSAATTK IGAGLWSQLL ISTNIFIKAI FTVTGKDMSV FMDQWVRTGG HAKFSLTSVF 550 560 570 580 590 600 NRKRNTIELE IRQDYVNQRG IRKYNGPLMV QLQELDGTFK HTLQIESTLV KSDITCHSKS 610 620 630 640 650 660 RRNKKKKIPL CTGEEVDMDL SAMDDSPVLW IRLDPEMILL RDLIIEQPDF QWQYQLRHER 670 680 690 700 710 720 DVTAQFQAIQ ALQKYPTNAT RLALTDTIES ERCFYQVRCE AAHSLTKVAN QMVASWSGPP 730 740 750 760 770 780 AMLNIFRKFF GSFSAPHIIK LNNFSNFQLY FLQKAIPVAM AGLRTSHGIC PPEVMRFLFD 790 800 810 820 830 840 LFKYNENSRN HYTDAYYRAA LVEALGETLT PVVSVAIHGT QITTDSLSTD AKLVLDEVTR 850 860 870 880 890 900 LLNMEKHLPS YKYMVSVSCL KVIRKLQKFG HLPSLPHIYR SYAEYGIYLD LRIAAMECLV 910 920 930 940 950 960 DFVKVDGRSE DLEHLITLLE TDPDPAARHA LAQLLIDNPP FTRESRSRLD KPNLVDRLWF 970 980 990 1000 1010 1020 SINRLPYDTK LRCDIVDLYY ALYGTKRPNC LQAGENQSFY KDLMKDNNSS VGSVTGSFKK 1030 1040 1050 1060 1070 1080 TSDSKSHLPT PTNTLDNEPQ ERQKPAMVTI KRTATEAFEV GDEIIKLERS EEITVLDEPV 1090 1100 1110 1120 1130 1140 NVQAYDSETK VNALQADEEA RDTHQAAKRL KNEMYAEDDN SSTMLDVGDS TRYESSHEEG 1150 1160 1170 1180 1190 1200 KLKSGDGGLK KKKKKEKKKH KHKHKHRHSK DKDKDKDKER KDKDKRDPHI SRLQARETAT 1210 1220 PDTLSSEDSS NSNSLPPMNL N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA." Verrijzer C.P., Yokomori K., Chen J.-L., Tjian R. Science 264:933-941(1994) [PubMed: 8178153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, INTERACTION WITH TBP AND TAF1. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[5]	"Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID." Yokomori K., Chen J.L., Admon A., Zhou S., Tjian R. Genes Dev. 7:2587-2597(1993) [PubMed: 8276241] [Abstract] Cited for: INTERACTION WITH TAF11 AND TAF12.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1135 AND SER-1136, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79243 mRNA. Translation: CAA55830.1. AE014296 Genomic DNA. Translation: AAF50190.2. AY070564 mRNA. Translation: AAL48035.1.
PIR	A54063.
RefSeq	NP_729571.1. NM_168387.1.
UniGene	Dm.6380.
3D structure databases
ProteinModelPortal	Q24325.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-255N.
IntAct	Q24325. 1 interaction.
STRING	Q24325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076361; FBpp0076090; FBgn0011836.
GeneID	39164.
KEGG	dme:Dmel_CG6711.
NMPDR	fig|7227.3.peg.9180.
Organism-specific databases
CTD	6873.
FlyBase	FBgn0011836. Taf2.
Phylogenomic databases
eggNOG	inNOG04181.
GeneTree	EMGT00050000009269.
InParanoid	Q24325.
OMA	CHSESKQ.
OrthoDB	EOG4PC86W.
PhylomeDB	Q24325.
Gene expression databases
Bgee	Q24325.
GermOnline	CG6711. Drosophila melanogaster.
Family and domain databases
InterPro	IPR014782. Peptidase_M1_N. [Graphical view]
KO	K03128.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	812285.

Entry information

Entry name

TAF2_DROME

Accession

Primary (citable) accession number: Q24325
Secondary accession number(s): Q8SZR7, Q9VT64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 27, 2003
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project