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Protein

Semaphorin-2A

Gene

Sema-2a

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in growth cones guidance. Required for both proper adult behavior and survival. Can function in vivo as a selective target-derived signal that inhibits the formation of specific synaptic terminal arbors.1 Publication

GO - Biological processi

  • adult behavior Source: FlyBase
  • axon guidance Source: FlyBase
  • dendrite guidance Source: FlyBase
  • drinking behavior Source: FlyBase
  • flight behavior Source: FlyBase
  • olfactory bulb axon guidance Source: FlyBase
  • salivary gland development Source: FlyBase
  • sensory neuron axon guidance Source: FlyBase
  • synaptic target inhibition Source: FlyBase
  • visual behavior Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-2A
Alternative name(s):
Semaphorin-II
Short name:
Sema II
Gene namesi
Name:Sema-2a
Synonyms:dSema-II
ORF Names:CG4700
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011260. Sema-2a.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
  • endomembrane system Source: FlyBase
  • extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 724699Semaphorin-2APRO_0000032301Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...)1 Publication
Disulfide bondi118 ↔ 129By similarity
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence analysis
Disulfide bondi291 ↔ 399By similarity
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi315 ↔ 358By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi525 ↔ 541By similarity
Disulfide bondi535 ↔ 550By similarity
Glycosylationi563 – 5631N-linked (GlcNAc...)Sequence analysis
Disulfide bondi590 ↔ 647By similarity
Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence analysis
Glycosylationi670 – 6701N-linked (GlcNAc...)Sequence analysis
Glycosylationi708 – 7081N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ24323.

Expressioni

Tissue specificityi

Transiently expressed by a single large muscle during motoneuron outgrowth and synapse formation.1 Publication

Developmental stagei

Expression begins around stage 10 in weak epidermal stripes. Nervous system expression is first detected around stage 15 and at stage 16 appears to remain restricted to a small subset of neurons. Beginning around stage 14, it is also expressed at a very high level in a single ventral thoracic muscle fiber in segment T3. It is also expressed in the embryonic gonads and in anterior sensory organs, including the maxillary complex.1 Publication

Gene expression databases

BgeeiQ24323.
ExpressionAtlasiQ24323. differential.
GenevisibleiQ24323. DM.

Interactioni

Subunit structurei

Interacts with PlexB.1 Publication

Protein-protein interaction databases

BioGridi62559. 17 interactions.
IntActiQ24323. 56 interactions.
MINTiMINT-868206.
STRINGi7227.FBpp0086237.

Structurei

3D structure databases

ProteinModelPortaliQ24323.
SMRiQ24323. Positions 52-647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 522478SemaPROSITE-ProRule annotationAdd
BLAST
Domaini552 – 663112Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Belongs to the semaphorin family.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
GeneTreeiENSGT00760000119134.
InParanoidiQ24323.
OMAiAVNHEHN.
OrthoDBiEOG7Z3F3W.
PhylomeDBiQ24323.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
SSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q24323-1) [UniParc]FASTAAdd to basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLQLSPLL ALLLLLCSSV SETAADYENT WNFYYERPCC TGNDQGNNNY
60 70 80 90 100
GKHGADHVRE FNCGKLYYRT FHMNEDRDTL YVGAMDRVFR VNLQNISSSN
110 120 130 140 150
CNRDVINLEP TRDDVVSCVS KGKSQIFDCK NHVRVIQSMD QGDRLYVCGT
160 170 180 190 200
NAHNPKDYVI YANLTHLPRS EYVIGVGLGI AKCPYDPLDN STAIYVENGN
210 220 230 240 250
PGGLPGLYSG TNAEFTKADT VIFRTDLYNT SAKRLEYKFK RTLKYDSKWL
260 270 280 290 300
DKPNFVGSFD IGEYVYFFFR ETAVEYINCG KAVYSRIARV CKKDVGGKNL
310 320 330 340 350
LAHNWATYLK ARLNCSISGE FPFYFNEIQS VYQLPSDKSR FFATFTTSTN
360 370 380 390 400
GLIGSAVCSF HINEIQAAFN GKFKEQSSSN SAWLPVLNSR VPEPRPGTCV
410 420 430 440 450
NDTSNLPDTV LNFIRSHPLM DKAVNHEHNN PVYYKRDLVF TKLVVDKIRI
460 470 480 490 500
DILNQEYIVY YVGTNLGRIY KIVQYYRNGE SLSKLLDIFE VAPNEAIQVM
510 520 530 540 550
EISQTRKSLY IGTDHRIKQI DLAMCNRRYD NCFRCVRDPY CGWDKEANTC
560 570 580 590 600
RPYELDLLQD VANETSDICD SSVLKKKIVV TYGQSVHLGC FVKIPEVLKN
610 620 630 640 650
EQVTWYHHSK DKGRYEIRYS PTKYIETTER GLVVVSVNEA DGGRYDCHLG
660 670 680 690 700
GSLLCSYNIT VDAHRCTPPN KSNDYQKIYS DWCHEFEKYK TAMKSWEKKQ
710 720
AQCSTRQNFS SNQHPNEIFR KPNV
Length:724
Mass (Da):82,998
Last modified:November 8, 2005 - v2
Checksum:i600DB0AEA82E677E
GO
Isoform C (identifier: Q24323-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MSLLQLSPLL...GNNNYGKHGA → MNGIHWLLFGSCLMLISQIEAVTEELSP

Note: No experimental confirmation available.
Show »
Length:697
Mass (Da):80,030
Checksum:i6E8321694556D24B
GO
Isoform D (identifier: Q24323-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-125: Missing.

Note: No experimental confirmation available.
Show »
Length:701
Mass (Da):80,470
Checksum:i1F6C577FF7144CAC
GO
Isoform E (identifier: Q24323-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-127: Missing.

Show »
Length:706
Mass (Da):81,050
Checksum:i57159ACA90818E93
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051V → A in AAC37187 (PubMed:8269517).Curated
Sequence conflicti110 – 1101P → S in AAR99105 (Ref. 5) Curated
Sequence conflicti701 – 7011A → G in AAC37187 (PubMed:8269517).Curated
Sequence conflicti711 – 7111S → C in AAC37187 (PubMed:8269517).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5555MSLLQ…GKHGA → MNGIHWLLFGSCLMLISQIE AVTEELSP in isoform C. CuratedVSP_016075Add
BLAST
Alternative sequencei103 – 12523Missing in isoform D. CuratedVSP_016076Add
BLAST
Alternative sequencei110 – 12718Missing in isoform E. 1 PublicationVSP_016077Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26083 mRNA. Translation: AAC37187.1.
AE013599 Genomic DNA. Translation: AAF57989.1.
AE013599 Genomic DNA. Translation: AAM68500.1.
AE013599 Genomic DNA. Translation: AAS64837.1.
AY095026 mRNA. Translation: AAM11354.1.
AY128437 mRNA. Translation: AAM75030.1.
BT011447 mRNA. Translation: AAR99105.1.
PIRiC49423.
RefSeqiNP_477507.1. NM_058159.5. [Q24323-1]
NP_599130.1. NM_134303.4. [Q24323-1]
NP_725586.1. NM_166178.3. [Q24323-2]
NP_995856.2. NM_206134.2. [Q24323-1]
UniGeneiDm.14046.

Genome annotation databases

EnsemblMetazoaiFBtr0087089; FBpp0086237; FBgn0011260. [Q24323-1]
FBtr0087090; FBpp0086238; FBgn0011260. [Q24323-1]
FBtr0345339; FBpp0311494; FBgn0011260. [Q24323-1]
GeneIDi36846.
KEGGidme:Dmel_CG4700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26083 mRNA. Translation: AAC37187.1.
AE013599 Genomic DNA. Translation: AAF57989.1.
AE013599 Genomic DNA. Translation: AAM68500.1.
AE013599 Genomic DNA. Translation: AAS64837.1.
AY095026 mRNA. Translation: AAM11354.1.
AY128437 mRNA. Translation: AAM75030.1.
BT011447 mRNA. Translation: AAR99105.1.
PIRiC49423.
RefSeqiNP_477507.1. NM_058159.5. [Q24323-1]
NP_599130.1. NM_134303.4. [Q24323-1]
NP_725586.1. NM_166178.3. [Q24323-2]
NP_995856.2. NM_206134.2. [Q24323-1]
UniGeneiDm.14046.

3D structure databases

ProteinModelPortaliQ24323.
SMRiQ24323. Positions 52-647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62559. 17 interactions.
IntActiQ24323. 56 interactions.
MINTiMINT-868206.
STRINGi7227.FBpp0086237.

Proteomic databases

PaxDbiQ24323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087089; FBpp0086237; FBgn0011260. [Q24323-1]
FBtr0087090; FBpp0086238; FBgn0011260. [Q24323-1]
FBtr0345339; FBpp0311494; FBgn0011260. [Q24323-1]
GeneIDi36846.
KEGGidme:Dmel_CG4700.

Organism-specific databases

CTDi36846.
FlyBaseiFBgn0011260. Sema-2a.

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
GeneTreeiENSGT00760000119134.
InParanoidiQ24323.
OMAiAVNHEHN.
OrthoDBiEOG7Z3F3W.
PhylomeDBiQ24323.

Miscellaneous databases

ChiTaRSiSema-2a. fly.
GenomeRNAii36846.
PROiQ24323.

Gene expression databases

BgeeiQ24323.
ExpressionAtlasiQ24323. differential.
GenevisibleiQ24323. DM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
SSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The semaphorin genes encode a family of transmembrane and secreted growth cone guidance molecules."
    Kolodkin A.L., Matthes D.J., Goodman C.S.
    Cell 75:1389-1399(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Drosophila Plexin B is a Sema-2a receptor required for axon guidance."
    Ayoob J.C., Terman J.R., Kolodkin A.L.
    Development 133:2125-2135(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLEXB.
  7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-95, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSEM2A_DROME
AccessioniPrimary (citable) accession number: Q24323
Secondary accession number(s): Q0E954
, Q6NN47, Q7KRC9, Q8MLF1, Q9V7Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 8, 2005
Last modified: June 8, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.