Q24319 (OST48_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Alternative name(s):
DmOST50
Short name=DrOST
OST5OP

Gene names

Name:	Ost48
Synonyms:	OST50
ORF Names:	CG9022

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi organization Inferred from mutant phenotype. Source: FlyBase lipid particle organization Inferred from mutant phenotype. Source: FlyBase protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lipid particle Inferred from direct assay. Source: FlyBase
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 449	431	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000021956
Regions
	Topological domain	19 – 412	394	Lumenal Potential
	Transmembrane	413 – 433	21	Helical; Potential
	Topological domain	434 – 449	16	Cytoplasmic Potential
Experimental info
	Sequence conflict	94	1	R → A in CAA57525. Ref.1
	Sequence conflict	148 – 149	2	EH → DD in CAA57079. Ref.5
	Sequence conflict	175 – 176	2	AA → R in CAA57079. Ref.5
	Sequence conflict	225 – 226	2	LL → VV in CAA57079. Ref.5
	Sequence conflict	287 – 289	3	RLR → QVG in CAA57079. Ref.5
	Sequence conflict	289	1	R → V in CAA57525. Ref.1
	Sequence conflict	304	1	P → T in CAA57079. Ref.5
	Sequence conflict	329 – 330	2	RA → AR in CAA57525. Ref.1
	Sequence conflict	335	1	D → A in CAA57079. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q24319 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: E4BC30AF376DCCD9	FASTA	449	49,981
	10 20 30 40 50 60 MMWKALLIAV LAIAHCQAVL ETDANTLVLL DNLAIRETHS IFFKSLQDRG FKLTYKLADD 70 80 90 100 110 120 SSLLLSKYGE YLYKNVIIFA PSVEEFGGDV SVERLAQFVD DGGNVLVAGS EKSGDALREF 130 140 150 160 170 180 ASECGFELDE ENAAVIDHLH YDVSDAGEHT TILTSAKNLI QADTIVGKAN RQADAAPLLY 190 200 210 220 230 240 RGTGLIADKE NPLVLKLLTA ESTAYSYNPE ASVSDYPHAV GRGTLLIAAL QARNNARVVF 250 260 270 280 290 300 SGSLLFFSDE SFTTAVQYAQ SGVFHKLAGN RDVAESISKW VFGETGRLRV ASVQHHKEGE 310 320 330 340 350 360 LLPPDQAYTI TDPVVYTIGI EELVQGEWRA FKASDIQLEF VRIDPFVRTY LKQTNTGAYQ 370 380 390 400 410 420 AKFKIPDVYG VYQFKVDYNR VGYTHLYSTT QVSVRPLEHT QYERFIPSAF PYYTSAFSMM 430 440 IGVFVFSFVF LHFKDEPVGR AAKEDKKSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PCR-mediated cloning and sequencing of the DmOST50 gene, a WBP1/AvOST50/OST48 homologue, from Drosophila melanogaster." Stagljar I., Te Heesen S., Aebi M. Gene 158:209-212(1995) [PubMed: 7607543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Structure and expression of histone H3.3 genes in Drosophila melanogaster and Drosophila hydei." Akhmanova A.S., Bindels P.S.T., Xu J., Miedema K., Kremer H., Hennig W. Genome 38:586-600(1995) [PubMed: 7557364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-449.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81999 mRNA. Translation: CAA57525.1. AE014298 Genomic DNA. Translation: AAF46453.1. BT010050 mRNA. Translation: AAQ22519.1. X81207 Genomic DNA. Translation: CAA57079.1.
PIR	JC4132.
RefSeq	NP_511096.2. NM_078541.3.
UniGene	Dm.2930.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1764327.
STRING	Q24319.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0071344; FBpp0071279; FBgn0014868.
GeneID	31849.
KEGG	dme:Dmel_CG9022.
NMPDR	fig|7227.3.peg.17103.
Organism-specific databases
CTD	31849.
FlyBase	FBgn0014868. Ost48.
Phylogenomic databases
eggNOG	inNOG05743.
GeneTree	EMGT00050000011193.
InParanoid	Q24319.
OMA	GRWVPFD.
OrthoDB	EOG4280H1.
PhylomeDB	Q24319.
Gene expression databases
ArrayExpress	Q24319.
Bgee	Q24319.
GermOnline	CG9022. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	775646.

Entry information

Entry name

OST48_DROME

Accession

Primary (citable) accession number: Q24319
Secondary accession number(s): Q24355, Q9W382

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project