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Protein

Cullin homolog 1

Gene

Cul1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of multiple SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. During early metamorphosis, part of the SCF-slmb complex that negatively regulates the InR/PI3K/TOR pathway to activate the pruning of unnecessary larval ddaC dendrites and mushroom body axons (PubMed:24068890). The SCF-slmb complex also regulates asymmetrical division of neuroblasts and inhibits ectopic neuroblast formation partly through SAK and Akt1 (PubMed:24413555). Also part of an SCF complex required for caspase activation during sperm differentiation (PubMed:20392747).3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: FlyBase

GO - Biological processi

  • mitotic G2 DNA damage checkpoint Source: FlyBase
  • negative regulation of apoptotic process Source: FlyBase
  • negative regulation of insulin receptor signaling pathway Source: FlyBase
  • negative regulation of TOR signaling Source: FlyBase
  • neuron remodeling Source: FlyBase
  • protein catabolic process Source: FlyBase
  • protein ubiquitination Source: FlyBase
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • regulation of glucose metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-69231. Cyclin D associated events in G1.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ24311.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin homolog 1
Alternative name(s):
Lin-19 homolog protein
Gene namesi
Name:Cul1
Synonyms:cul-1, lin19
ORF Names:CG1877
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015509. Cul1.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: In the spermatid, colocalizes with ntc at the actin-based individualization complex and the cystic bulge.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: FlyBase
  • SCF ubiquitin ligase complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Severe dendrite pruning defects in ddaC neurons at 16 hours after puparium formation (APF) and axon pruning defects in mushroom body gamma neurons at 24 hours APF. RNAi-mediated knockdown results in a significant increase in Akt1 protein levels and activity in ddaC somas (PubMed:24068890). In the larval brain there is a large increase in the number of neuroblasts, 40% of which show a spindle misorientation at metaphase (PubMed:24413555).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197861 – 774Cullin homolog 1Add BLAST774

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki718Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ24311.
PRIDEiQ24311.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiFBgn0015509.
GenevisibleiQ24311. DM.

Interactioni

Subunit structurei

Component of SCF E3 ubiquitin-protein ligase complexes consisting of Skpa, Cul1, Roc1a and an F-box protein. In larval neuroblast self renewal and asymmetric division, as well as ddaC dendrite and mushroom body axon pruning, the complex contains the F-box protein slmb (SCF-slmb) (PubMed:24068890, PubMed:24413555). In caspase activation during sperm differentiation, the complex contains the F-box protein ntc (PubMed:20392747). Interacts directly with Roc1a (PubMed:11500045). Interacts with Fsn (PubMed:20123973).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rca1P916662EBI-136038,EBI-7085629
SkpAO774302EBI-136038,EBI-180180

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: FlyBase

Protein-protein interaction databases

BioGridi61606. 20 interactors.
DIPiDIP-19461N.
IntActiQ24311. 7 interactors.
MINTiMINT-989395.
STRINGi7227.FBpp0087921.

Structurei

3D structure databases

ProteinModelPortaliQ24311.
SMRiQ24311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000021491.
InParanoidiQ24311.
KOiK03347.
OMAiYLHESTL.
OrthoDBiEOG091G02WR.
PhylomeDBiQ24311.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSGNSQTT QKLVNLDDIW SELVEGIMQV FEHEKSLTRS QYMRFYTHVY
60 70 80 90 100
DYCTSVSAAP SGRSSGKTGG AQLVGKKLYD RLEQFLKSYL SELLTKFKAI
110 120 130 140 150
SGEEVLLSRY TKQWKSYQFS STVLDGICNY LNRNWVKREC EEGQKGIYKI
160 170 180 190 200
YRLALVAWKG HLFQVLNEPV TKAVLKSIEE ERQGKLINRS LVRDVIECYV
210 220 230 240 250
ELSFNEEDTD AEQQKLSVYK QNFENKFIAD TSAFYEKESD AFLSTNTVTE
260 270 280 290 300
YLKHVENRLE EETQRVRGFN SKNGLSYLHE TTADVLKSTC EEVLIEKHLK
310 320 330 340 350
IFHTEFQNLL NADRNDDLKR MYSLVALSSK NLTDLKSILE NHILHQGTEA
360 370 380 390 400
IAKCCTTDAA NDPKTYVQTI LDVHKKYNAL VLTAFNNDNG FVAALDKACG
410 420 430 440 450
KFINSNVVTI ANSASKSPEL LAKYCDLLLK KSSKNPEDKE LEDNLNQVMV
460 470 480 490 500
VFKYIEDKDV FQKYYSKMLA KRLVNHTSAS DDAEAMMISK LKQTCGYEYT
510 520 530 540 550
VKLQRMFQDI GVSKDLNSYF KQYLAEKNLT MEIDFGIEVL SSGSWPFQLS
560 570 580 590 600
NNFLLPSELE RSVRQFNEFY AARHSGRKLN WLYQMCKGEL IMNVNRNNSS
610 620 630 640 650
TYTLQASTFQ MSVLLQFNDQ LSFTVQQLQD NTQTQQENLI QVLQILLKAK
660 670 680 690 700
VLTSSDNENS LTPESTVELF LDYKNKKRRI NINQPLKTEL KVEQETVHKH
710 720 730 740 750
IEEDRKLLIQ AAIVRIMKMR KRLNHTNLIS EVLNQLSTRF KPKVPVIKKC
760 770
IDILIEKEYL ERMEGHKDTY SYLA
Length:774
Mass (Da):89,511
Last modified:November 2, 2001 - v2
Checksum:i1AF0D035DE85363C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266Missing in AAA85085 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41642 mRNA. Translation: AAA85085.1.
AF136343 mRNA. Translation: AAD33676.1.
AE013599 Genomic DNA. Translation: AAF59174.1.
AE013599 Genomic DNA. Translation: AAM68871.1.
AE013599 Genomic DNA. Translation: AAM68872.1.
BT010290 mRNA. Translation: AAQ23608.1.
RefSeqiNP_523655.1. NM_078931.4.
NP_724621.1. NM_165569.2.
NP_724622.1. NM_165570.3.
NP_724623.1. NM_165571.2.
UniGeneiDm.7926.

Genome annotation databases

EnsemblMetazoaiFBtr0088845; FBpp0087921; FBgn0015509.
FBtr0088846; FBpp0087922; FBgn0015509.
FBtr0088847; FBpp0087923; FBgn0015509.
FBtr0088848; FBpp0087924; FBgn0015509.
GeneIDi35742.
KEGGidme:Dmel_CG1877.
UCSCiCG1877-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41642 mRNA. Translation: AAA85085.1.
AF136343 mRNA. Translation: AAD33676.1.
AE013599 Genomic DNA. Translation: AAF59174.1.
AE013599 Genomic DNA. Translation: AAM68871.1.
AE013599 Genomic DNA. Translation: AAM68872.1.
BT010290 mRNA. Translation: AAQ23608.1.
RefSeqiNP_523655.1. NM_078931.4.
NP_724621.1. NM_165569.2.
NP_724622.1. NM_165570.3.
NP_724623.1. NM_165571.2.
UniGeneiDm.7926.

3D structure databases

ProteinModelPortaliQ24311.
SMRiQ24311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61606. 20 interactors.
DIPiDIP-19461N.
IntActiQ24311. 7 interactors.
MINTiMINT-989395.
STRINGi7227.FBpp0087921.

Proteomic databases

PaxDbiQ24311.
PRIDEiQ24311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088845; FBpp0087921; FBgn0015509.
FBtr0088846; FBpp0087922; FBgn0015509.
FBtr0088847; FBpp0087923; FBgn0015509.
FBtr0088848; FBpp0087924; FBgn0015509.
GeneIDi35742.
KEGGidme:Dmel_CG1877.
UCSCiCG1877-RA. d. melanogaster.

Organism-specific databases

CTDi8454.
FlyBaseiFBgn0015509. Cul1.

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000021491.
InParanoidiQ24311.
KOiK03347.
OMAiYLHESTL.
OrthoDBiEOG091G02WR.
PhylomeDBiQ24311.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-69231. Cyclin D associated events in G1.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ24311.

Miscellaneous databases

GenomeRNAii35742.
PROiQ24311.

Gene expression databases

BgeeiFBgn0015509.
GenevisibleiQ24311. DM.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUL1_DROME
AccessioniPrimary (citable) accession number: Q24311
Secondary accession number(s): A4UZ78, Q0E9G0, Q9V312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2001
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.