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Protein

Homeobox protein B-H2

Gene

B-H2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

B-H1 and B-H2 are regulated by members of the wg signaling pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally required in R1 and R6 receptor cells and primary pigment cells for normal eye development. Coexpression is also required for the fate determination of external sensory organs, formation of notal microchaetae, formation of presutural macrochaetae, antennal development and for distal leg morphogenesis; segmentation and specification of tarsal segments 3-5.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi380 – 43960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • chaeta morphogenesis Source: UniProtKB
  • compound eye photoreceptor cell differentiation Source: FlyBase
  • eye-antennal disc morphogenesis Source: UniProtKB
  • eye pigment granule organization Source: UniProtKB
  • lateral inhibition Source: FlyBase
  • leg disc proximal/distal pattern formation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • sensory organ boundary specification Source: UniProtKB
  • visual perception Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Sensory transduction, Vision, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein B-H2
Alternative name(s):
Homeobox protein BarH2
Gene namesi
Name:B-H2
Synonyms:BarH2
ORF Names:CG5488
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004854. B-H2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Homeobox protein B-H2PRO_0000048833Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei593 – 5931Phosphothreonine1 Publication
Modified residuei595 – 5951Phosphoserine1 Publication
Modified residuei602 – 6021Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24256.

PTM databases

iPTMnetiQ24256.

Expressioni

Tissue specificityi

B-H1 and B-H2 are abundant in the eye-antenna imaginal disk. Expressed in R1 and R6 cells throughout larval stage until 30 hours after puparium formation, at which time expression is seen in the anterior and posterior primary pigment cells. Coexpressed in embryonic glial cells, neurons of the CNS and PNS, most latitudinal anterior cells of the developing notum and the central circular region of the leg and antennal imaginal disk throughout larval development.4 Publications

Developmental stagei

Coexpressed at high levels with B-H1 in embryo and pupae and at low levels in larvae.1 Publication

Gene expression databases

BgeeiFBgn0004854.
ExpressionAtlasiQ24256. differential.
GenevisibleiQ24256. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0074203.

Structurei

3D structure databases

ProteinModelPortaliQ24256.
SMRiQ24256. Positions 377-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 8576Ala-richAdd
BLAST
Compositional biasi101 – 204104Gln-richAdd
BLAST
Compositional biasi107 – 256150His-richAdd
BLAST
Compositional biasi272 – 32150Asn-richAdd
BLAST
Compositional biasi283 – 37795Ser-richAdd
BLAST
Compositional biasi447 – 578132Ala-richAdd
BLAST
Compositional biasi607 – 64438Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the Antp homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0488. Eukaryota.
ENOG411188D. LUCA.
GeneTreeiENSGT00840000129678.
InParanoidiQ24256.
OMAiEREREHY.
OrthoDBiEOG091G13SM.
PhylomeDBiQ24256.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMPPEMSA TTAAPVGSAP SATAHHPAAV GGGMPRPASP AVGSNTTTAT
60 70 80 90 100
ATTATRSRFM ITDILAGAAA ASAAAAAAAA ALAAASSGGG RGSPTDSERE
110 120 130 140 150
QSLVAQHHHH HQQQQQHHHH QQQQQQQQHQ QAALQQYIVQ QQQLLRFERE
160 170 180 190 200
REREREREHY RERHSPPGNN PYAHHPMPPH LLAHFPPAHY AVLQQQQQQQ
210 220 230 240 250
QQQQHPHPHH LQLERERLEA LHRHGHGLTG DPAQHLSHLS HLSHQQHHPH
260 270 280 290 300
LHHPMHDERS RSPLMLQQLG GNGGNNNNNN NNSSSASNNN NNNNNSASAN
310 320 330 340 350
SNIISGNSSS SNNNNGSGNG NMLLGGPGSS ISGDQASTID DSDSDDCGGK
360 370 380 390 400
DDDGDDSMKN GSSANGDSSS HLSLSLSKKQ RKARTAFTDH QLQTLEKSFE
410 420 430 440 450
RQKYLSVQDR MELANKLELS DCQVKTWYQN RRTKWKRQTA VGLELLAEAG
460 470 480 490 500
NYAAFQRLYG GATPYLSAWP YAAAAAAQSP HGATPSAIDI YYRQAAAAAA
510 520 530 540 550
MQKPSLPASY RMYQSSIPPG MSLPGMPAPP PPGAAPMLSG YYAAAAAAAA
560 570 580 590 600
SAGAQQQQQQ PPAASRSPAT SQSANSEADC ERTSSSSRQR LITPSPPLNP
610 620 630 640
GSPPHRERIN EEEDRERDEE RDIERERERE RERDEDDEEE LALEV
Length:645
Mass (Da):69,951
Last modified:June 1, 2003 - v3
Checksum:iCF99E12F7ECBB133
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1233Missing in AAB59218 (PubMed:1346120).Curated
Sequence conflicti229 – 2291T → P in AAB59218 (PubMed:1346120).Curated
Sequence conflicti288 – 2892Missing in AAB59218 (PubMed:1346120).Curated
Sequence conflicti375 – 3751S → N in AAB59218 (PubMed:1346120).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82884 mRNA. Translation: AAA28383.2.
M82887, M82885, M82886 Genomic DNA. Translation: AAB59218.1.
AE014298 Genomic DNA. Translation: AAF48725.1.
BT022144 mRNA. Translation: AAY51539.1.
PIRiA41726.
S27806.
RefSeqiNP_523386.1. NM_078662.3.
UniGeneiDm.2611.

Genome annotation databases

EnsemblMetazoaiFBtr0074429; FBpp0074203; FBgn0004854.
GeneIDi32723.
KEGGidme:Dmel_CG5488.
UCSCiCG5488-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82884 mRNA. Translation: AAA28383.2.
M82887, M82885, M82886 Genomic DNA. Translation: AAB59218.1.
AE014298 Genomic DNA. Translation: AAF48725.1.
BT022144 mRNA. Translation: AAY51539.1.
PIRiA41726.
S27806.
RefSeqiNP_523386.1. NM_078662.3.
UniGeneiDm.2611.

3D structure databases

ProteinModelPortaliQ24256.
SMRiQ24256. Positions 377-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0074203.

PTM databases

iPTMnetiQ24256.

Proteomic databases

PaxDbiQ24256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074429; FBpp0074203; FBgn0004854.
GeneIDi32723.
KEGGidme:Dmel_CG5488.
UCSCiCG5488-RA. d. melanogaster.

Organism-specific databases

CTDi32723.
FlyBaseiFBgn0004854. B-H2.

Phylogenomic databases

eggNOGiKOG0488. Eukaryota.
ENOG411188D. LUCA.
GeneTreeiENSGT00840000129678.
InParanoidiQ24256.
OMAiEREREHY.
OrthoDBiEOG091G13SM.
PhylomeDBiQ24256.

Miscellaneous databases

ChiTaRSiB-H2. fly.
GenomeRNAii32723.
PROiQ24256.

Gene expression databases

BgeeiFBgn0004854.
ExpressionAtlasiQ24256. differential.
GenevisibleiQ24256. DM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBARH2_DROME
AccessioniPrimary (citable) accession number: Q24256
Secondary accession number(s): Q24257, Q4V712, Q9VX55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.