Q24246 (DYIN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytoplasmic dynein 1 intermediate chain
Short name=DH IC

Alternative name(s):
Dynein intermediate chain, cytosolic
Protein short wing

Gene names

Name:	sw
Synonyms:	Cdic, Dic19B
ORF Names:	CG18000

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the help dynein bind to dynactin 150 kDa component By similarity. Ref.2
Subunit structure	Homodimer By similarity. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs) By similarity.
Subcellular location	Cytoplasm › cytoskeleton Ref.1. Isoform 2c: Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Aggregates in cytoplasm around lysosomes. Ref.1 Isoform 2a: Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Aggregates in cytoplasm around the nucleus. Ref.1 Isoform 2b: Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Aggregates in cytoplasm around the nucleus. Ref.1
Tissue specificity	High levels of isoform 1b, isoform 1c, isoform 3a and isoform 4 accumulate in early egg chambers and at stage 9 become concentrated at the posterior of the oocyte. Isoform 5a and isoform 5b are highly expressed in adult head and to a lesser extent in adult torso. Isoform 1a, isoform 2a and isoform 2b are found in all tissues examined, including ovaries, midgut, torso and head. Ref.1 Ref.2
Developmental stage	Expressed both maternally and zygotically. Abundant in embryos and adults, low levels in larva and pupae. Isoform 1a, isoform 2a and isoform 2b are constitutively expressed at high levels and isoform 2c at low levels in embryos and adults. Ref.1
Sequence similarities	Belongs to the dynein intermediate chain family. Contains 7 WD repeats.
Sequence caution	The sequence AAC70942.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAC78306.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton Dynein Lysosome Membrane Microtubule Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Molecular function	Motor protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon cargo transport Inferred from mutant phenotype PubMed 18758451. Source: FlyBase centrosome localization Inferred from mutant phenotype PubMed 15888542. Source: FlyBase cytoplasmic transport Inferred from mutant phenotype PubMed 16077093. Source: FlyBase eye photoreceptor cell differentiation Inferred from genetic interaction PubMed 15329347. Source: FlyBase protein localization to kinetochore Inferred from mutant phenotype PubMed 16105886. Source: FlyBase sperm individualization Inferred from mutant phenotype PubMed 15829565. Source: FlyBase spindle organization Inferred from mutant phenotype PubMed 15888542. Source: FlyBase
Cellular_component	cytoplasmic dynein complex Inferred from electronic annotation. Source: InterPro lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from direct assay PubMed 18433294. Source: FlyBase nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 11 isoforms produced by alternative splicing. [Align] [Select]

Isoform 5a (identifier: Q24246-11)

Also known as: J;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1a (identifier: Q24246-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     144-187: VLSCHSSPLSGYMEDWWRPRKAHATDYYDEYNLNPGLEWEDEFT → AHATDYYVLAFDAQG

Isoform 1b (identifier: Q24246-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     144-187: VLSCHSSPLSGYMEDWWRPRKAHATDYYDEYNLNPGLEWEDEFT → AHATDYYG

Isoform 1c (identifier: Q24246-4)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     144-187: VLSCHSSPLSGYMEDWWRPRKAHATDYYDEYNLNPGLEWEDEFT → AHATDYY

Isoform 2a (identifier: Q24246-5)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     144-164: Missing.
     187-187: T → TG

Isoform 2b (identifier: Q24246-6)

Also known as: E;

The sequence of this isoform differs from the canonical sequence as follows:
     144-164: Missing.

Isoform 2c (identifier: Q24246-7)

Also known as: F;

The sequence of this isoform differs from the canonical sequence as follows:
     144-164: Missing.
     187-187: T → TVLAFDAQG

Isoform 3a (identifier: Q24246-8)

Also known as: G;

The sequence of this isoform differs from the canonical sequence as follows:
     144-171: Missing.
     187-187: T → TG

Isoform 3b (identifier: Q24246-9)

Also known as: H;

The sequence of this isoform differs from the canonical sequence as follows:
     144-171: Missing.

Isoform 4 (identifier: Q24246-10)

Also known as: I;

The sequence of this isoform differs from the canonical sequence as follows:
     146-187: SCHSSPLSGYMEDWWRPRKAHATDYYDEYNLNPGLEWEDEFT → AFDAQG

Isoform 5b (identifier: Q24246-12)

Also known as: K;

The sequence of this isoform differs from the canonical sequence as follows:
     144-153: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 663

663

Cytoplasmic dynein 1 intermediate chain

PRO_0000114661

Regions

Repeat

311 – 360

50

WD 1

Repeat

364 – 404

41

WD 2

Repeat

413 – 454

42

WD 3

Repeat

463 – 503

41

WD 4

Repeat

508 – 553

46

WD 5

Repeat

556 – 596

41

WD 6

Repeat

602 – 641

40

WD 7

Natural variations

Alternative sequence

144 – 187

44

VLSCH…EDEFT → AHATDYYVLAFDAQG in isoform 1a.

VSP_001345

Alternative sequence

144 – 187

44

VLSCH…EDEFT → AHATDYYG in isoform 1b.

VSP_001346

Alternative sequence

144 – 187

44

VLSCH…EDEFT → AHATDYY in isoform 1c.

VSP_001347

Alternative sequence

144 – 171

28

Missing in isoform 3a and isoform 3b.

VSP_001342

Alternative sequence

144 – 164

21

Missing in isoform 2a, isoform 2b and isoform 2c.

VSP_007686

Alternative sequence

144 – 153

10

Missing in isoform 5b.

VSP_007685

Alternative sequence

146 – 187

42

SCHSS…EDEFT → AFDAQG in isoform 4.

VSP_001343

Alternative sequence

187

1

T → TG in isoform 2a and isoform 3a.

VSP_001348

Alternative sequence

187

1

T → TVLAFDAQG in isoform 2c.

VSP_007687

Experimental info

Sequence conflict

47

1

D → G in AAC78306. Ref.1

Sequence conflict

47

1

D → G in AAC70933. Ref.1

Sequence conflict

47

1

D → G in AAC70934. Ref.1

Sequence conflict

47

1

D → G in AAC70935. Ref.1

Sequence conflict

47

1

D → G in AAC70936. Ref.1

Sequence conflict

47

1

D → G in AAC70937. Ref.1

Sequence conflict

47

1

D → G in AAC70938. Ref.1

Sequence conflict

47

1

D → G in AAC70939. Ref.1

Sequence conflict

47

1

D → G in AAC70940. Ref.1

Sequence conflict

47

1

D → G in AAC70941. Ref.1

Sequence conflict

47

1

D → G in AAC70942. Ref.1

Sequence conflict

47

1

D → G in AAC70943. Ref.1

Sequence conflict

139 – 145

7

GGNGDVL → AETAMV in AAB51185. Ref.6

Sequence conflict

235

1

V → L in AAB51185. Ref.6

Sequence conflict

296 – 297

2

HA → SM in AAB51185. Ref.6

Sequence conflict

521 – 522

2

NQ → KP in AAB51185. Ref.6

Sequence conflict

537 – 548

12

DWTIK…SLKDT → KIWSLKLPISSCQFSVVSGI NSN in AAB51185. Ref.6

Sequence conflict

579

1

G → A in AAB51185. Ref.6

Sequence conflict

593

1

E → K in AAB51185. Ref.6

Sequence conflict

628

1

L → Q in AAB51185. Ref.6

Sequence conflict

645 – 663

19

WSRFN…QSDEV → IKMNQSVRSRTI in AAB51185. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

663

Helix Strand Turn

Details...

Beta strand

110 – 112

3

Beta strand

128 – 133

6

Helix

244 – 251

8

Helix

254 – 273

20

Sequences

	Sequence		Length	Mass (Da)
	Isoform 5a (J) [UniParc]. Last modified July 3, 2003. Version 3. Checksum: 4C726D4C5F47FAF7	FASTA	663	73,917
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDVLSCHSS PLSGYMEDWW RPRKAHATDY YDEYNLNPGL 190 200 210 220 230 240 EWEDEFTDDE ESSLQNLGNG FTSKLPPGYL THGLPTVKDV APAITPLEIK KETEVKKEVN 250 260 270 280 290 300 ELSEEQKQMI ILSENFQRFV VRAGRVIERA LSENVDIYTD YIGGGDSEEA NDERSHARLS 310 320 330 340 350 360 LNRVFYDERW SKNRCITSMD WSTHFPELVV GSYHNNEESP NEPDGVVMVW NTKFKKSTPE 370 380 390 400 410 420 DVFHCQSAVM STCFAKFNPN LILGGTYSGQ IVLWDNRVQK RTPIQRTPLS AAAHTHPVYC 430 440 450 460 470 480 LQMVGTQNAH NVISISSDGK LCSWSLDMLS QPQDTLELQQ RQSKAIAITS MAFPANEINS 490 500 510 520 530 540 LVMGSEDGYV YSASRHGLRS GVNEVYERHL GPITGISTHY NQLSPDFGHL FLTSSIDWTI 550 560 570 580 590 600 KLWSLKDTKP LYSFEDNSDY VMDVAWSPVH PALFAAVDGS GRLDLWNLNQ DTEVPTASIV 610 620 630 640 650 660 VAGAPALNRV SWTPSGLHVC IGDEAGKLYV YDVAENLAQP SRDEWSRFNT HLSEIKMNQS DEV
	Isoform 1a (A) [UniParc]. Checksum: F3267D4090F25E05	FASTA	634	70,249
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY VLAFDAQGDD EESSLQNLGN GFTSKLPPGY 190 200 210 220 230 240 LTHGLPTVKD VAPAITPLEI KKETEVKKEV NELSEEQKQM IILSENFQRF VVRAGRVIER 250 260 270 280 290 300 ALSENVDIYT DYIGGGDSEE ANDERSHARL SLNRVFYDER WSKNRCITSM DWSTHFPELV 310 320 330 340 350 360 VGSYHNNEES PNEPDGVVMV WNTKFKKSTP EDVFHCQSAV MSTCFAKFNP NLILGGTYSG 370 380 390 400 410 420 QIVLWDNRVQ KRTPIQRTPL SAAAHTHPVY CLQMVGTQNA HNVISISSDG KLCSWSLDML 430 440 450 460 470 480 SQPQDTLELQ QRQSKAIAIT SMAFPANEIN SLVMGSEDGY VYSASRHGLR SGVNEVYERH 490 500 510 520 530 540 LGPITGISTH YNQLSPDFGH LFLTSSIDWT IKLWSLKDTK PLYSFEDNSD YVMDVAWSPV 550 560 570 580 590 600 HPALFAAVDG SGRLDLWNLN QDTEVPTASI VVAGAPALNR VSWTPSGLHV CIGDEAGKLY 610 620 630 VYDVAENLAQ PSRDEWSRFN THLSEIKMNQ SDEV
	Isoform 1b (B) [UniParc]. Checksum: AB436F70A431915B	FASTA	627	69,505
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY GDDEESSLQN LGNGFTSKLP PGYLTHGLPT 190 200 210 220 230 240 VKDVAPAITP LEIKKETEVK KEVNELSEEQ KQMIILSENF QRFVVRAGRV IERALSENVD 250 260 270 280 290 300 IYTDYIGGGD SEEANDERSH ARLSLNRVFY DERWSKNRCI TSMDWSTHFP ELVVGSYHNN 310 320 330 340 350 360 EESPNEPDGV VMVWNTKFKK STPEDVFHCQ SAVMSTCFAK FNPNLILGGT YSGQIVLWDN 370 380 390 400 410 420 RVQKRTPIQR TPLSAAAHTH PVYCLQMVGT QNAHNVISIS SDGKLCSWSL DMLSQPQDTL 430 440 450 460 470 480 ELQQRQSKAI AITSMAFPAN EINSLVMGSE DGYVYSASRH GLRSGVNEVY ERHLGPITGI 490 500 510 520 530 540 STHYNQLSPD FGHLFLTSSI DWTIKLWSLK DTKPLYSFED NSDYVMDVAW SPVHPALFAA 550 560 570 580 590 600 VDGSGRLDLW NLNQDTEVPT ASIVVAGAPA LNRVSWTPSG LHVCIGDEAG KLYVYDVAEN 610 620 LAQPSRDEWS RFNTHLSEIK MNQSDEV
	Isoform 1c (C) [UniParc]. Checksum: D96D9CD9AA89814A	FASTA	626	69,447
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY DDEESSLQNL GNGFTSKLPP GYLTHGLPTV 190 200 210 220 230 240 KDVAPAITPL EIKKETEVKK EVNELSEEQK QMIILSENFQ RFVVRAGRVI ERALSENVDI 250 260 270 280 290 300 YTDYIGGGDS EEANDERSHA RLSLNRVFYD ERWSKNRCIT SMDWSTHFPE LVVGSYHNNE 310 320 330 340 350 360 ESPNEPDGVV MVWNTKFKKS TPEDVFHCQS AVMSTCFAKF NPNLILGGTY SGQIVLWDNR 370 380 390 400 410 420 VQKRTPIQRT PLSAAAHTHP VYCLQMVGTQ NAHNVISISS DGKLCSWSLD MLSQPQDTLE 430 440 450 460 470 480 LQQRQSKAIA ITSMAFPANE INSLVMGSED GYVYSASRHG LRSGVNEVYE RHLGPITGIS 490 500 510 520 530 540 THYNQLSPDF GHLFLTSSID WTIKLWSLKD TKPLYSFEDN SDYVMDVAWS PVHPALFAAV 550 560 570 580 590 600 DGSGRLDLWN LNQDTEVPTA SIVVAGAPAL NRVSWTPSGL HVCIGDEAGK LYVYDVAENL 610 620 AQPSRDEWSR FNTHLSEIKM NQSDEV
	Isoform 2a (D) [UniParc]. Checksum: 900A25F62B24A451	FASTA	643	71,458
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY DEYNLNPGLE WEDEFTGDDE ESSLQNLGNG 190 200 210 220 230 240 FTSKLPPGYL THGLPTVKDV APAITPLEIK KETEVKKEVN ELSEEQKQMI ILSENFQRFV 250 260 270 280 290 300 VRAGRVIERA LSENVDIYTD YIGGGDSEEA NDERSHARLS LNRVFYDERW SKNRCITSMD 310 320 330 340 350 360 WSTHFPELVV GSYHNNEESP NEPDGVVMVW NTKFKKSTPE DVFHCQSAVM STCFAKFNPN 370 380 390 400 410 420 LILGGTYSGQ IVLWDNRVQK RTPIQRTPLS AAAHTHPVYC LQMVGTQNAH NVISISSDGK 430 440 450 460 470 480 LCSWSLDMLS QPQDTLELQQ RQSKAIAITS MAFPANEINS LVMGSEDGYV YSASRHGLRS 490 500 510 520 530 540 GVNEVYERHL GPITGISTHY NQLSPDFGHL FLTSSIDWTI KLWSLKDTKP LYSFEDNSDY 550 560 570 580 590 600 VMDVAWSPVH PALFAAVDGS GRLDLWNLNQ DTEVPTASIV VAGAPALNRV SWTPSGLHVC 610 620 630 640 IGDEAGKLYV YDVAENLAQP SRDEWSRFNT HLSEIKMNQS DEV
	Isoform 2b (E) [UniParc]. Checksum: 10271A56BFBC8B62	FASTA	642	71,400
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY DEYNLNPGLE WEDEFTDDEE SSLQNLGNGF 190 200 210 220 230 240 TSKLPPGYLT HGLPTVKDVA PAITPLEIKK ETEVKKEVNE LSEEQKQMII LSENFQRFVV 250 260 270 280 290 300 RAGRVIERAL SENVDIYTDY IGGGDSEEAN DERSHARLSL NRVFYDERWS KNRCITSMDW 310 320 330 340 350 360 STHFPELVVG SYHNNEESPN EPDGVVMVWN TKFKKSTPED VFHCQSAVMS TCFAKFNPNL 370 380 390 400 410 420 ILGGTYSGQI VLWDNRVQKR TPIQRTPLSA AAHTHPVYCL QMVGTQNAHN VISISSDGKL 430 440 450 460 470 480 CSWSLDMLSQ PQDTLELQQR QSKAIAITSM AFPANEINSL VMGSEDGYVY SASRHGLRSG 490 500 510 520 530 540 VNEVYERHLG PITGISTHYN QLSPDFGHLF LTSSIDWTIK LWSLKDTKPL YSFEDNSDYV 550 560 570 580 590 600 MDVAWSPVHP ALFAAVDGSG RLDLWNLNQD TEVPTASIVV AGAPALNRVS WTPSGLHVCI 610 620 630 640 GDEAGKLYVY DVAENLAQPS RDEWSRFNTH LSEIKMNQSD EV
	Isoform 2c (F) [UniParc]. Checksum: 937440311CA820DE	FASTA	650	72,202
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDAHATDYY DEYNLNPGLE WEDEFTVLAF DAQGDDEESS 190 200 210 220 230 240 LQNLGNGFTS KLPPGYLTHG LPTVKDVAPA ITPLEIKKET EVKKEVNELS EEQKQMIILS 250 260 270 280 290 300 ENFQRFVVRA GRVIERALSE NVDIYTDYIG GGDSEEANDE RSHARLSLNR VFYDERWSKN 310 320 330 340 350 360 RCITSMDWST HFPELVVGSY HNNEESPNEP DGVVMVWNTK FKKSTPEDVF HCQSAVMSTC 370 380 390 400 410 420 FAKFNPNLIL GGTYSGQIVL WDNRVQKRTP IQRTPLSAAA HTHPVYCLQM VGTQNAHNVI 430 440 450 460 470 480 SISSDGKLCS WSLDMLSQPQ DTLELQQRQS KAIAITSMAF PANEINSLVM GSEDGYVYSA 490 500 510 520 530 540 SRHGLRSGVN EVYERHLGPI TGISTHYNQL SPDFGHLFLT SSIDWTIKLW SLKDTKPLYS 550 560 570 580 590 600 FEDNSDYVMD VAWSPVHPAL FAAVDGSGRL DLWNLNQDTE VPTASIVVAG APALNRVSWT 610 620 630 640 650 PSGLHVCIGD EAGKLYVYDV AENLAQPSRD EWSRFNTHLS EIKMNQSDEV
	Isoform 3a (G) [UniParc]. Checksum: 10882999594FBDD9	FASTA	636	70,636
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDDEYNLNP GLEWEDEFTG DDEESSLQNL GNGFTSKLPP 190 200 210 220 230 240 GYLTHGLPTV KDVAPAITPL EIKKETEVKK EVNELSEEQK QMIILSENFQ RFVVRAGRVI 250 260 270 280 290 300 ERALSENVDI YTDYIGGGDS EEANDERSHA RLSLNRVFYD ERWSKNRCIT SMDWSTHFPE 310 320 330 340 350 360 LVVGSYHNNE ESPNEPDGVV MVWNTKFKKS TPEDVFHCQS AVMSTCFAKF NPNLILGGTY 370 380 390 400 410 420 SGQIVLWDNR VQKRTPIQRT PLSAAAHTHP VYCLQMVGTQ NAHNVISISS DGKLCSWSLD 430 440 450 460 470 480 MLSQPQDTLE LQQRQSKAIA ITSMAFPANE INSLVMGSED GYVYSASRHG LRSGVNEVYE 490 500 510 520 530 540 RHLGPITGIS THYNQLSPDF GHLFLTSSID WTIKLWSLKD TKPLYSFEDN SDYVMDVAWS 550 560 570 580 590 600 PVHPALFAAV DGSGRLDLWN LNQDTEVPTA SIVVAGAPAL NRVSWTPSGL HVCIGDEAGK 610 620 630 LYVYDVAENL AQPSRDEWSR FNTHLSEIKM NQSDEV
	Isoform 3b (H) [UniParc]. Checksum: 422B7524D4A50381	FASTA	635	70,579
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDDEYNLNP GLEWEDEFTD DEESSLQNLG NGFTSKLPPG 190 200 210 220 230 240 YLTHGLPTVK DVAPAITPLE IKKETEVKKE VNELSEEQKQ MIILSENFQR FVVRAGRVIE 250 260 270 280 290 300 RALSENVDIY TDYIGGGDSE EANDERSHAR LSLNRVFYDE RWSKNRCITS MDWSTHFPEL 310 320 330 340 350 360 VVGSYHNNEE SPNEPDGVVM VWNTKFKKST PEDVFHCQSA VMSTCFAKFN PNLILGGTYS 370 380 390 400 410 420 GQIVLWDNRV QKRTPIQRTP LSAAAHTHPV YCLQMVGTQN AHNVISISSD GKLCSWSLDM 430 440 450 460 470 480 LSQPQDTLEL QQRQSKAIAI TSMAFPANEI NSLVMGSEDG YVYSASRHGL RSGVNEVYER 490 500 510 520 530 540 HLGPITGIST HYNQLSPDFG HLFLTSSIDW TIKLWSLKDT KPLYSFEDNS DYVMDVAWSP 550 560 570 580 590 600 VHPALFAAVD GSGRLDLWNL NQDTEVPTAS IVVAGAPALN RVSWTPSGLH VCIGDEAGKL 610 620 630 YVYDVAENLA QPSRDEWSRF NTHLSEIKMN QSDEV
	Isoform 4 (I) [UniParc]. Checksum: 6D17F8435B8C8BF8	FASTA	627	69,428
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDVLAFDAQ GDDEESSLQN LGNGFTSKLP PGYLTHGLPT 190 200 210 220 230 240 VKDVAPAITP LEIKKETEVK KEVNELSEEQ KQMIILSENF QRFVVRAGRV IERALSENVD 250 260 270 280 290 300 IYTDYIGGGD SEEANDERSH ARLSLNRVFY DERWSKNRCI TSMDWSTHFP ELVVGSYHNN 310 320 330 340 350 360 EESPNEPDGV VMVWNTKFKK STPEDVFHCQ SAVMSTCFAK FNPNLILGGT YSGQIVLWDN 370 380 390 400 410 420 RVQKRTPIQR TPLSAAAHTH PVYCLQMVGT QNAHNVISIS SDGKLCSWSL DMLSQPQDTL 430 440 450 460 470 480 ELQQRQSKAI AITSMAFPAN EINSLVMGSE DGYVYSASRH GLRSGVNEVY ERHLGPITGI 490 500 510 520 530 540 STHYNQLSPD FGHLFLTSSI DWTIKLWSLK DTKPLYSFED NSDYVMDVAW SPVHPALFAA 550 560 570 580 590 600 VDGSGRLDLW NLNQDTEVPT ASIVVAGAPA LNRVSWTPSG LHVCIGDEAG KLYVYDVAEN 610 620 LAQPSRDEWS RFNTHLSEIK MNQSDEV
	Isoform 5b (K) [UniParc]. Checksum: 3073C8F8F3B2FB59	FASTA	653	72,906
	10 20 30 40 50 60 MDRKAELERK KAKLAALREE KDRRRREKEI KDMEEAAGRI GGGAGIDKDQ RKDLDEMLSS 70 80 90 100 110 120 LGVAPVSEVL SSLSSVNSMT SDNSNTQTPD ASLQATVNGQ SGGKKQPLNL SVYNVQATNI 130 140 150 160 170 180 PPKETLVYTK QTQTTSTGGG NGDGYMEDWW RPRKAHATDY YDEYNLNPGL EWEDEFTDDE 190 200 210 220 230 240 ESSLQNLGNG FTSKLPPGYL THGLPTVKDV APAITPLEIK KETEVKKEVN ELSEEQKQMI 250 260 270 280 290 300 ILSENFQRFV VRAGRVIERA LSENVDIYTD YIGGGDSEEA NDERSHARLS LNRVFYDERW 310 320 330 340 350 360 SKNRCITSMD WSTHFPELVV GSYHNNEESP NEPDGVVMVW NTKFKKSTPE DVFHCQSAVM 370 380 390 400 410 420 STCFAKFNPN LILGGTYSGQ IVLWDNRVQK RTPIQRTPLS AAAHTHPVYC LQMVGTQNAH 430 440 450 460 470 480 NVISISSDGK LCSWSLDMLS QPQDTLELQQ RQSKAIAITS MAFPANEINS LVMGSEDGYV 490 500 510 520 530 540 YSASRHGLRS GVNEVYERHL GPITGISTHY NQLSPDFGHL FLTSSIDWTI KLWSLKDTKP 550 560 570 580 590 600 LYSFEDNSDY VMDVAWSPVH PALFAAVDGS GRLDLWNLNQ DTEVPTASIV VAGAPALNRV 610 620 630 640 650 SWTPSGLHVC IGDEAGKLYV YDVAENLAQP SRDEWSRFNT HLSEIKMNQS DEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing." Nurminsky D.I., Nurminskaya M.V., Benevolenskaya E.V., Shevelyov Y.Y., Hartl D.L., Gvozdev V.A. Mol. Cell. Biol. 18:6816-6825(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1A; 1B; 1C; 2A; 2B; 2C; 3A; 3B; 4; 5A AND 5B), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Ovary.
[2]	"A molecular genetic analysis of the interaction between the cytoplasmic dynein intermediate chain and the glued (Dynactin) complex." Boylan K., Serr M., Hays T.S. Mol. Biol. Cell 11:3791-3803(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2B), FUNCTION, TISSUE SPECIFICITY. Tissue: Ovary.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1B). Strain: Berkeley. Tissue: Embryo.
[6]	"Cluster of tandem repeats in Drosophila genome comprising putative genes encoding cytoplasmic dynein intermediate chain and 3'-part of annexin X." Benevolenskaya E.V., Gvozdev V.A. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 101-663 (ISOFORM 4). Strain: GT WA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF070687 Genomic DNA. Translation: AAC78306.1. Sequence problems.
AF070689 mRNA. Translation: AAC70933.1.
AF070690 mRNA. Translation: AAC70934.1.
AF070691 mRNA. Translation: AAC70935.1.
AF070692 mRNA. Translation: AAC70936.1.
AF070693 mRNA. Translation: AAC70937.1.
AF070694 mRNA. Translation: AAC70938.1.
AF070695 mRNA. Translation: AAC70939.1.
AF070696 mRNA. Translation: AAC70940.1.
AF070697 mRNA. Translation: AAC70941.1.
AF070698 mRNA. Translation: AAC70942.1. Sequence problems.
AF070699 mRNA. Translation: AAC70943.1.
AF263371 mRNA. Translation: AAF73046.1.
AE014298 Genomic DNA. Translation: AAF50928.2.
AE014298 Genomic DNA. Translation: AAN09553.1.
AE014298 Genomic DNA. Translation: AAN09554.1.
AE014298 Genomic DNA. Translation: AAN09555.1.
AE014298 Genomic DNA. Translation: AAN09556.1.
AE014298 Genomic DNA. Translation: AAN09557.1.
AE014298 Genomic DNA. Translation: AAN09558.1.
AE014298 Genomic DNA. Translation: AAN09559.1.
AE014298 Genomic DNA. Translation: AAN09560.1.
AE014298 Genomic DNA. Translation: AAN09561.1.
AE014298 Genomic DNA. Translation: AAN09562.1.
BT016101 mRNA. Translation: AAV36986.1.
L41945 Genomic DNA. Translation: AAB51185.1.

RefSeq

NP_477069.1. NM_057721.3.
NP_477070.1. NM_057722.3.
NP_477071.1. NM_057723.3.
NP_477072.1. NM_057724.3.
NP_477073.1. NM_057725.3.
NP_477074.1. NM_057726.3.
NP_477075.2. NM_057727.4.
NP_477076.1. NM_057728.3.
NP_477077.1. NM_057729.3.
NP_477078.1. NM_057730.4.
NP_477079.1. NM_057731.3.
NP_996521.1. NM_206798.1.
NP_996522.1. NM_206799.1.

UniGene

Dm.20780.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P2T	X-ray	3.00	C	123-138	[»]
3FM7	X-ray	3.50	C/D	109-135	[»]
3L9K	X-ray	3.00	W/X/Y/Z	242-279	[»]

ProteinModelPortal

Q24246.

SMR

Q24246. Positions 109-135, 242-279, 320-637.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-19664N.

IntAct

Q24246. 1 interaction.

MINT

MINT-1717948.

Proteomic databases

PaxDb

Q24246.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0089406; FBpp0088428; FBgn0003654.

GeneID

44160.

KEGG

dme:Dmel_CG18000.

Organism-specific databases

CTD

44160.

FlyBase

FBgn0003654. sw.

Phylogenomic databases

eggNOG

NOG308180.

GeneTree

ENSGT00690000101848.

InParanoid

Q24246.

KO

K10415.

OMA

SAGHEDS.

OrthoDB

EOG4WWQ0J.

PhylomeDB

Q24246.

Gene expression databases

Bgee

Q24246.

GermOnline

CG18000. Drosophila melanogaster.

Family and domain databases

Gene3D

2.130.10.10. 1 hit.

InterPro

IPR025956. Dynein_IC_1/2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]

SMART

SM00320. WD40. 6 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SLC4A1. drosophila.

EvolutionaryTrace

Q24246.

GenomeRNAi

44160.

NextBio

836907.

Entry information

Entry name

DYIN_DROME

Accession

Primary (citable) accession number: Q24246
Secondary accession number(s): O96508 , O96510, O96511, O96512, O96513, O96514, O96515, O96516, Q5U0Z1, Q86BQ5, Q9NG49, Q9TZR7, Q9TZR8, Q9TZR9, Q9TZS0, Q9VR78

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 3, 2003
Last modified:	May 1, 2013
This is version 138 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project