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Q24238

- APH4_DROME

UniProt

Q24238 - APH4_DROME

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Protein
Alkaline phosphatase 4
Gene
Aph-4, CG1462
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Important role in neural and renal epithelial function.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion By similarity.
Binds 2 zinc ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi93 – 931Magnesium By similarity
Metal bindingi93 – 931Zinc 2 By similarity
Active sitei144 – 1441Phosphoserine intermediate By similarityBy similarity
Metal bindingi202 – 2021Magnesium By similarity
Metal bindingi204 – 2041Magnesium By similarity
Metal bindingi369 – 3691Magnesium By similarity
Metal bindingi374 – 3741Zinc 1 By similarity
Metal bindingi378 – 3781Zinc 1 By similarity
Metal bindingi415 – 4151Zinc 2 By similarity
Metal bindingi416 – 4161Zinc 2 By similarity
Metal bindingi504 – 5041Zinc 1 By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. epithelial fluid transport Source: UniProtKB
  2. multicellular organism reproduction Source: FlyBase
  3. nervous system development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 4 (EC:3.1.3.1)
Gene namesi
Name:Aph-4
ORF Names:CG1462
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0016123. Aph-4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei571 – 59121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: FlyBase
  3. extracellular space Source: FlyBase
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 570550Alkaline phosphatase 4
PRO_0000024049Add
BLAST
Propeptidei571 – 59626Removed in mature form
PRO_0000024050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi262 – 2621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi401 – 4011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi464 – 4641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi470 – 4701N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi539 ↔ 550 By similarityBy similarity
Lipidationi570 – 5701GPI-anchor amidated asparagine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ24238.

Expressioni

Tissue specificityi

Ellipsoid body ring neurons in the adult brain and in the lower Malpighian tubule and ureter.1 Publication

Developmental stagei

Highest abundance during larval stage (prior to the secretion of pupal cuticle) and adult stage.1 Publication

Gene expression databases

BgeeiQ24238.

Interactioni

Subunit structurei

Homodimer By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ24238.
SMRiQ24238. Positions 50-545.

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000028185.
InParanoidiQ24238.
KOiK01077.
OMAiWYADANM.
OrthoDBiEOG7SN8CH.
PhylomeDBiQ24238.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A1 Publication (identifier: Q24238-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MHCLVILGFL LGSLVAFSWA GVTTQPPPLI RTLSAGGDIG PQFDVGKTKE    50
PEDAEFWHNV GLRQLEKTIK QAQRVKEDSY QKKARNIIIF IGDGMGISTI 100
SAGRIYKGQY LKHGYGEEET LVFDDFPNTG MAKTYNVDKQ VPDSAGTATA 150
IFSGSKTHYG AIGMDATRSK KNGQQGRVQS VMEWAQKEGK RTGVVTTTRI 200
THATPAATYA HIYDRDWECD TEVPAESVGF HVDIARQLVE NAPGNRFNVI 250
LGGGMSPMGI LNASEVKTTI FEGPTETICT RGDNRNLPAE WLAHHANDTV 300
PPALVHNRKD LLNVNVKKVD HLMGLFRNNH ITYSIAREAG EPSLQEMTET 350
ALGILERGDE SNGFVLLVEG GRIDQGHHMN YARAALHELY EFDLAIQAAV 400
NNTDPDETLI LVTADHSHAV TFNGYALRGA DILGTANSHE KNDPMFYETI 450
SYANGPGYWD HLANDSRPQN SSNMWMPLKH FTAEERAAPT YRHLATVPRK 500
DETHGGEDVA VFAYGPGSSL IRGVFEQNYL AYVMSYAGCL GPAKDFDDSC 550
EDHKDGQKDR PLDKPNPKRN GATVVGASLI PILTAATAAI LRGRGL 596
Length:596
Mass (Da):65,262
Last modified:March 28, 2003 - v3
Checksum:i333F3345BEFBAEFB
GO
Isoform B1 Publication (identifier: Q24238-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.

Show »
Length:502
Mass (Da):54,887
Checksum:iA9E70E72FDAC8AAF
GO

Sequence cautioni

The sequence CAA67052.1 differs from that shown. Reason: Frameshift at position 538.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9494Missing in isoform B. 1 Publication
VSP_007002Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001I → N in AAL68351. 1 Publication
Sequence conflicti280 – 2801T → S in ABA81845. 1 Publication
Sequence conflicti358 – 3581G → D in CAA67052. 1 Publication
Sequence conflicti375 – 3751Q → H in CAA67052. 1 Publication
Sequence conflicti495 – 4962AT → EP in CAA67052. 1 Publication
Sequence conflicti570 – 5701N → S in CAA67052. 1 Publication
Sequence conflicti573 – 5731T → S in CAA67052. 1 Publication
Sequence conflicti593 – 5942GR → CH in CAA67052. 1 Publication
Sequence conflicti593 – 5942GR → CH in ABA81845. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98402 mRNA. Translation: CAA67052.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1.
AE014297 Genomic DNA. Translation: AAN14265.1.
AY075544 mRNA. Translation: AAL68351.1.
BT023911 mRNA. Translation: ABA81845.1.
BT057987 mRNA. Translation: ACM16697.1.
RefSeqiNP_524601.2. NM_079862.3. [Q24238-1]
NP_733413.1. NM_170534.2. [Q24238-2]
UniGeneiDm.5439.

Genome annotation databases

EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123. [Q24238-1]
GeneIDi43671.
KEGGidme:Dmel_CG1462.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98402 mRNA. Translation: CAA67052.1 . Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1 .
AE014297 Genomic DNA. Translation: AAN14265.1 .
AY075544 mRNA. Translation: AAL68351.1 .
BT023911 mRNA. Translation: ABA81845.1 .
BT057987 mRNA. Translation: ACM16697.1 .
RefSeqi NP_524601.2. NM_079862.3. [Q24238-1 ]
NP_733413.1. NM_170534.2. [Q24238-2 ]
UniGenei Dm.5439.

3D structure databases

ProteinModelPortali Q24238.
SMRi Q24238. Positions 50-545.
ModBasei Search...

Proteomic databases

PaxDbi Q24238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0085733 ; FBpp0085095 ; FBgn0016123 . [Q24238-1 ]
GeneIDi 43671.
KEGGi dme:Dmel_CG1462.

Organism-specific databases

CTDi 43671.
FlyBasei FBgn0016123. Aph-4.

Phylogenomic databases

eggNOGi COG1785.
GeneTreei ENSGT00390000008704.
HOGENOMi HOG000028185.
InParanoidi Q24238.
KOi K01077.
OMAi WYADANM.
OrthoDBi EOG7SN8CH.
PhylomeDBi Q24238.

Miscellaneous databases

GenomeRNAii 43671.
NextBioi 835185.

Gene expression databases

Bgeei Q24238.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Drosophila alkaline phosphatase specific to the ellipsoid body of the adult brain and the lower Malpighian (renal) tubule."
    Yang M.Y., Wang Z., MacPherson M., Dow J.A.T., Kaiser K.
    Genetics 154:285-297(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Head.
  5. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
    Strain: Berkeley.
    Tissue: Testis.

Entry informationi

Entry nameiAPH4_DROME
AccessioniPrimary (citable) accession number: Q24238
Secondary accession number(s): B9EQR2
, Q3KN28, Q8IMH0, Q8SXW6, Q9VA19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: July 9, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi