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Protein

Alkaline phosphatase 4

Gene

Aph4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Important role in neural and renal epithelial function.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi93MagnesiumBy similarity1
Metal bindingi93Zinc 2By similarity1
Active sitei144Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi202MagnesiumBy similarity1
Metal bindingi204MagnesiumBy similarity1
Metal bindingi369MagnesiumBy similarity1
Metal bindingi374Zinc 1By similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi415Zinc 2By similarity1
Metal bindingi416Zinc 2By similarity1
Metal bindingi504Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: FlyBase
  • epithelial fluid transport Source: UniProtKB
  • multicellular organism reproduction Source: FlyBase
  • nervous system development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-1483166. Synthesis of PA.
R-DME-6811438. Intra-Golgi traffic.
R-DME-8935690. Miscellaneous digestion events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 4 (EC:3.1.3.1)
Gene namesi
Name:Aph4Imported
Synonyms:Aph-4Imported
ORF Names:CG1462Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0016123. Aph4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei571 – 591HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002404921 – 570Alkaline phosphatase 4Add BLAST550
PropeptideiPRO_0000024050571 – 596Removed in mature formAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi401N-linked (GlcNAc...)Sequence analysis1
Glycosylationi464N-linked (GlcNAc...)Sequence analysis1
Glycosylationi470N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi539 ↔ 550By similarity
Lipidationi570GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ24238.
PRIDEiQ24238.

Expressioni

Tissue specificityi

Ellipsoid body ring neurons in the adult brain and in the lower Malpighian tubule and ureter.1 Publication

Developmental stagei

Highest abundance during larval stage (prior to the secretion of pupal cuticle) and adult stage.1 Publication

Gene expression databases

BgeeiFBgn0016123.
GenevisibleiQ24238. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ24238. 1 interactor.
STRINGi7227.FBpp0085095.

Structurei

3D structure databases

ProteinModelPortaliQ24238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000028185.
InParanoidiQ24238.
KOiK01077.
OMAiHSPERNW.
OrthoDBiEOG091G067H.
PhylomeDBiQ24238.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A1 Publication (identifier: Q24238-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHCLVILGFL LGSLVAFSWA GVTTQPPPLI RTLSAGGDIG PQFDVGKTKE
60 70 80 90 100
PEDAEFWHNV GLRQLEKTIK QAQRVKEDSY QKKARNIIIF IGDGMGISTI
110 120 130 140 150
SAGRIYKGQY LKHGYGEEET LVFDDFPNTG MAKTYNVDKQ VPDSAGTATA
160 170 180 190 200
IFSGSKTHYG AIGMDATRSK KNGQQGRVQS VMEWAQKEGK RTGVVTTTRI
210 220 230 240 250
THATPAATYA HIYDRDWECD TEVPAESVGF HVDIARQLVE NAPGNRFNVI
260 270 280 290 300
LGGGMSPMGI LNASEVKTTI FEGPTETICT RGDNRNLPAE WLAHHANDTV
310 320 330 340 350
PPALVHNRKD LLNVNVKKVD HLMGLFRNNH ITYSIAREAG EPSLQEMTET
360 370 380 390 400
ALGILERGDE SNGFVLLVEG GRIDQGHHMN YARAALHELY EFDLAIQAAV
410 420 430 440 450
NNTDPDETLI LVTADHSHAV TFNGYALRGA DILGTANSHE KNDPMFYETI
460 470 480 490 500
SYANGPGYWD HLANDSRPQN SSNMWMPLKH FTAEERAAPT YRHLATVPRK
510 520 530 540 550
DETHGGEDVA VFAYGPGSSL IRGVFEQNYL AYVMSYAGCL GPAKDFDDSC
560 570 580 590
EDHKDGQKDR PLDKPNPKRN GATVVGASLI PILTAATAAI LRGRGL
Length:596
Mass (Da):65,262
Last modified:March 28, 2003 - v3
Checksum:i333F3345BEFBAEFB
GO
Isoform B1 Publication (identifier: Q24238-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.1 Publication
Show »
Length:502
Mass (Da):54,887
Checksum:iA9E70E72FDAC8AAF
GO

Sequence cautioni

The sequence CAA67052 differs from that shown. Reason: Frameshift at position 538.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200I → N in AAL68351 (PubMed:12537569).Curated1
Sequence conflicti280T → S in ABA81845 (Ref. 5) Curated1
Sequence conflicti358G → D in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti375Q → H in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti495 – 496AT → EP in CAA67052 (PubMed:10628988).Curated2
Sequence conflicti570N → S in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti573T → S in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti593 – 594GR → CH in CAA67052 (PubMed:10628988).Curated2
Sequence conflicti593 – 594GR → CH in ABA81845 (Ref. 5) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0070021 – 94Missing in isoform B. 2 PublicationsAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98402 mRNA. Translation: CAA67052.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1.
AE014297 Genomic DNA. Translation: AAN14265.1.
AY075544 mRNA. Translation: AAL68351.1.
BT023911 mRNA. Translation: ABA81845.1.
BT057987 mRNA. Translation: ACM16697.1.
RefSeqiNP_524601.2. NM_079862.3. [Q24238-1]
NP_733413.1. NM_170534.2. [Q24238-2]
UniGeneiDm.5439.

Genome annotation databases

EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123. [Q24238-1]
GeneIDi43671.
KEGGidme:Dmel_CG1462.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98402 mRNA. Translation: CAA67052.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1.
AE014297 Genomic DNA. Translation: AAN14265.1.
AY075544 mRNA. Translation: AAL68351.1.
BT023911 mRNA. Translation: ABA81845.1.
BT057987 mRNA. Translation: ACM16697.1.
RefSeqiNP_524601.2. NM_079862.3. [Q24238-1]
NP_733413.1. NM_170534.2. [Q24238-2]
UniGeneiDm.5439.

3D structure databases

ProteinModelPortaliQ24238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ24238. 1 interactor.
STRINGi7227.FBpp0085095.

Proteomic databases

PaxDbiQ24238.
PRIDEiQ24238.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123. [Q24238-1]
GeneIDi43671.
KEGGidme:Dmel_CG1462.

Organism-specific databases

CTDi43671.
FlyBaseiFBgn0016123. Aph4.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000028185.
InParanoidiQ24238.
KOiK01077.
OMAiHSPERNW.
OrthoDBiEOG091G067H.
PhylomeDBiQ24238.

Enzyme and pathway databases

ReactomeiR-DME-1483166. Synthesis of PA.
R-DME-6811438. Intra-Golgi traffic.
R-DME-8935690. Miscellaneous digestion events.

Miscellaneous databases

GenomeRNAii43671.
PROiQ24238.

Gene expression databases

BgeeiFBgn0016123.
GenevisibleiQ24238. DM.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPH4_DROME
AccessioniPrimary (citable) accession number: Q24238
Secondary accession number(s): B9EQR2
, Q3KN28, Q8IMH0, Q8SXW6, Q9VA19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 30, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.