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Protein

Alkaline phosphatase 4

Gene

Alp4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Important role in neural and renal epithelial function.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi93MagnesiumBy similarity1
Metal bindingi93Zinc 2By similarity1
Active sitei144Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi202MagnesiumBy similarity1
Metal bindingi204MagnesiumBy similarity1
Metal bindingi369MagnesiumBy similarity1
Metal bindingi374Zinc 1By similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi415Zinc 2By similarity1
Metal bindingi416Zinc 2By similarity1
Metal bindingi504Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: FlyBase
  • epithelial fluid transport Source: UniProtKB
  • multicellular organism reproduction Source: FlyBase
  • nervous system development Source: UniProtKB

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-1483166 Synthesis of PA
R-DME-8935690 Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 41 Publication (EC:3.1.3.1)
Gene namesi
Name:Alp4Imported
Synonyms:aph-41 Publication
ORF Names:CG1462Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0016123 Alp4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei571 – 591HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002404921 – 570Alkaline phosphatase 4Add BLAST550
PropeptideiPRO_0000024050571 – 596Removed in mature formAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi464N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi470N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi539 ↔ 550By similarity
Lipidationi570GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ24238
PRIDEiQ24238

Expressioni

Tissue specificityi

Ellipsoid body ring neurons in the adult brain and in the lower Malpighian tubule and ureter.1 Publication

Developmental stagei

Highest abundance during larval stage (prior to the secretion of pupal cuticle) and adult stage.1 Publication

Gene expression databases

BgeeiFBgn0016123
GenevisibleiQ24238 DM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ24238, 1 interactor
STRINGi7227.FBpp0085095

Structurei

3D structure databases

ProteinModelPortaliQ24238
SMRiQ24238
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000028185
InParanoidiQ24238
KOiK01077
OMAiFEQNYIA
OrthoDBiEOG091G067H
PhylomeDBiQ24238

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A1 Publication (identifier: Q24238-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHCLVILGFL LGSLVAFSWA GVTTQPPPLI RTLSAGGDIG PQFDVGKTKE
60 70 80 90 100
PEDAEFWHNV GLRQLEKTIK QAQRVKEDSY QKKARNIIIF IGDGMGISTI
110 120 130 140 150
SAGRIYKGQY LKHGYGEEET LVFDDFPNTG MAKTYNVDKQ VPDSAGTATA
160 170 180 190 200
IFSGSKTHYG AIGMDATRSK KNGQQGRVQS VMEWAQKEGK RTGVVTTTRI
210 220 230 240 250
THATPAATYA HIYDRDWECD TEVPAESVGF HVDIARQLVE NAPGNRFNVI
260 270 280 290 300
LGGGMSPMGI LNASEVKTTI FEGPTETICT RGDNRNLPAE WLAHHANDTV
310 320 330 340 350
PPALVHNRKD LLNVNVKKVD HLMGLFRNNH ITYSIAREAG EPSLQEMTET
360 370 380 390 400
ALGILERGDE SNGFVLLVEG GRIDQGHHMN YARAALHELY EFDLAIQAAV
410 420 430 440 450
NNTDPDETLI LVTADHSHAV TFNGYALRGA DILGTANSHE KNDPMFYETI
460 470 480 490 500
SYANGPGYWD HLANDSRPQN SSNMWMPLKH FTAEERAAPT YRHLATVPRK
510 520 530 540 550
DETHGGEDVA VFAYGPGSSL IRGVFEQNYL AYVMSYAGCL GPAKDFDDSC
560 570 580 590
EDHKDGQKDR PLDKPNPKRN GATVVGASLI PILTAATAAI LRGRGL
Length:596
Mass (Da):65,262
Last modified:March 28, 2003 - v3
Checksum:i333F3345BEFBAEFB
GO
Isoform B1 Publication (identifier: Q24238-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.1 Publication
Show »
Length:502
Mass (Da):54,887
Checksum:iA9E70E72FDAC8AAF
GO

Sequence cautioni

The sequence CAA67052 differs from that shown. Reason: Frameshift at position 538.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200I → N in AAL68351 (PubMed:12537569).Curated1
Sequence conflicti280T → S in ABA81845 (Ref. 5) Curated1
Sequence conflicti358G → D in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti375Q → H in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti495 – 496AT → EP in CAA67052 (PubMed:10628988).Curated2
Sequence conflicti570N → S in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti573T → S in CAA67052 (PubMed:10628988).Curated1
Sequence conflicti593 – 594GR → CH in CAA67052 (PubMed:10628988).Curated2
Sequence conflicti593 – 594GR → CH in ABA81845 (Ref. 5) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0070021 – 94Missing in isoform B. 2 PublicationsAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98402 mRNA Translation: CAA67052.1 Frameshift.
AE014297 Genomic DNA Translation: AAF57106.1
AE014297 Genomic DNA Translation: AAN14265.1
AY075544 mRNA Translation: AAL68351.1
BT023911 mRNA Translation: ABA81845.1
BT057987 mRNA Translation: ACM16697.1
RefSeqiNP_524601.2, NM_079862.3 [Q24238-1]
NP_733413.1, NM_170534.2 [Q24238-2]
UniGeneiDm.5439

Genome annotation databases

EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123 [Q24238-1]
FBtr0085734; FBpp0085096; FBgn0016123 [Q24238-2]
GeneIDi43671
KEGGidme:Dmel_CG1462

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPH4_DROME
AccessioniPrimary (citable) accession number: Q24238
Secondary accession number(s): B9EQR2
, Q3KN28, Q8IMH0, Q8SXW6, Q9VA19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: March 28, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health