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Q24238

- APH4_DROME

UniProt

Q24238 - APH4_DROME

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Protein

Alkaline phosphatase 4

Gene

Aph-4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Important role in neural and renal epithelial function.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Binds 1 magnesium ion.By similarity
Binds 2 zinc ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi93 – 931MagnesiumBy similarity
Metal bindingi93 – 931Zinc 2By similarity
Active sitei144 – 1441Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi202 – 2021MagnesiumBy similarity
Metal bindingi204 – 2041MagnesiumBy similarity
Metal bindingi369 – 3691MagnesiumBy similarity
Metal bindingi374 – 3741Zinc 1By similarity
Metal bindingi378 – 3781Zinc 1By similarity
Metal bindingi415 – 4151Zinc 2By similarity
Metal bindingi416 – 4161Zinc 2By similarity
Metal bindingi504 – 5041Zinc 1By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. epithelial fluid transport Source: UniProtKB
  2. multicellular organism reproduction Source: FlyBase
  3. nervous system development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 4 (EC:3.1.3.1)
Gene namesi
Name:Aph-4
ORF Names:CG1462
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0016123. Aph-4.

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: FlyBase
  3. extracellular space Source: FlyBase
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 570550Alkaline phosphatase 4PRO_0000024049Add
BLAST
Propeptidei571 – 59626Removed in mature formPRO_0000024050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi539 ↔ 550By similarity
Lipidationi570 – 5701GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ24238.

Expressioni

Tissue specificityi

Ellipsoid body ring neurons in the adult brain and in the lower Malpighian tubule and ureter.1 Publication

Developmental stagei

Highest abundance during larval stage (prior to the secretion of pupal cuticle) and adult stage.1 Publication

Gene expression databases

BgeeiQ24238.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ24238.
SMRiQ24238. Positions 50-545.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei571 – 59121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000028185.
InParanoidiQ24238.
KOiK01077.
OMAiWYADANM.
OrthoDBiEOG7SN8CH.
PhylomeDBiQ24238.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A1 Publication (identifier: Q24238-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHCLVILGFL LGSLVAFSWA GVTTQPPPLI RTLSAGGDIG PQFDVGKTKE
60 70 80 90 100
PEDAEFWHNV GLRQLEKTIK QAQRVKEDSY QKKARNIIIF IGDGMGISTI
110 120 130 140 150
SAGRIYKGQY LKHGYGEEET LVFDDFPNTG MAKTYNVDKQ VPDSAGTATA
160 170 180 190 200
IFSGSKTHYG AIGMDATRSK KNGQQGRVQS VMEWAQKEGK RTGVVTTTRI
210 220 230 240 250
THATPAATYA HIYDRDWECD TEVPAESVGF HVDIARQLVE NAPGNRFNVI
260 270 280 290 300
LGGGMSPMGI LNASEVKTTI FEGPTETICT RGDNRNLPAE WLAHHANDTV
310 320 330 340 350
PPALVHNRKD LLNVNVKKVD HLMGLFRNNH ITYSIAREAG EPSLQEMTET
360 370 380 390 400
ALGILERGDE SNGFVLLVEG GRIDQGHHMN YARAALHELY EFDLAIQAAV
410 420 430 440 450
NNTDPDETLI LVTADHSHAV TFNGYALRGA DILGTANSHE KNDPMFYETI
460 470 480 490 500
SYANGPGYWD HLANDSRPQN SSNMWMPLKH FTAEERAAPT YRHLATVPRK
510 520 530 540 550
DETHGGEDVA VFAYGPGSSL IRGVFEQNYL AYVMSYAGCL GPAKDFDDSC
560 570 580 590
EDHKDGQKDR PLDKPNPKRN GATVVGASLI PILTAATAAI LRGRGL
Length:596
Mass (Da):65,262
Last modified:March 28, 2003 - v3
Checksum:i333F3345BEFBAEFB
GO
Isoform B1 Publication (identifier: Q24238-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.1 Publication

Show »
Length:502
Mass (Da):54,887
Checksum:iA9E70E72FDAC8AAF
GO

Sequence cautioni

The sequence CAA67052.1 differs from that shown. Reason: Frameshift at position 538.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001I → N in AAL68351. (PubMed:12537569)Curated
Sequence conflicti280 – 2801T → S in ABA81845. 1 PublicationCurated
Sequence conflicti358 – 3581G → D in CAA67052. (PubMed:10628988)Curated
Sequence conflicti375 – 3751Q → H in CAA67052. (PubMed:10628988)Curated
Sequence conflicti495 – 4962AT → EP in CAA67052. (PubMed:10628988)Curated
Sequence conflicti570 – 5701N → S in CAA67052. (PubMed:10628988)Curated
Sequence conflicti573 – 5731T → S in CAA67052. (PubMed:10628988)Curated
Sequence conflicti593 – 5942GR → CH in CAA67052. (PubMed:10628988)Curated
Sequence conflicti593 – 5942GR → CH in ABA81845. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9494Missing in isoform B. 2 PublicationsVSP_007002Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98402 mRNA. Translation: CAA67052.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1.
AE014297 Genomic DNA. Translation: AAN14265.1.
AY075544 mRNA. Translation: AAL68351.1.
BT023911 mRNA. Translation: ABA81845.1.
BT057987 mRNA. Translation: ACM16697.1.
RefSeqiNP_524601.2. NM_079862.3. [Q24238-1]
NP_733413.1. NM_170534.2. [Q24238-2]
UniGeneiDm.5439.

Genome annotation databases

EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123. [Q24238-1]
GeneIDi43671.
KEGGidme:Dmel_CG1462.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98402 mRNA. Translation: CAA67052.1 . Frameshift.
AE014297 Genomic DNA. Translation: AAF57106.1 .
AE014297 Genomic DNA. Translation: AAN14265.1 .
AY075544 mRNA. Translation: AAL68351.1 .
BT023911 mRNA. Translation: ABA81845.1 .
BT057987 mRNA. Translation: ACM16697.1 .
RefSeqi NP_524601.2. NM_079862.3. [Q24238-1 ]
NP_733413.1. NM_170534.2. [Q24238-2 ]
UniGenei Dm.5439.

3D structure databases

ProteinModelPortali Q24238.
SMRi Q24238. Positions 50-545.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q24238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0085733 ; FBpp0085095 ; FBgn0016123 . [Q24238-1 ]
GeneIDi 43671.
KEGGi dme:Dmel_CG1462.

Organism-specific databases

CTDi 43671.
FlyBasei FBgn0016123. Aph-4.

Phylogenomic databases

eggNOGi COG1785.
GeneTreei ENSGT00390000008704.
HOGENOMi HOG000028185.
InParanoidi Q24238.
KOi K01077.
OMAi WYADANM.
OrthoDBi EOG7SN8CH.
PhylomeDBi Q24238.

Miscellaneous databases

GenomeRNAii 43671.
NextBioi 835185.

Gene expression databases

Bgeei Q24238.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Drosophila alkaline phosphatase specific to the ellipsoid body of the adult brain and the lower Malpighian (renal) tubule."
    Yang M.Y., Wang Z., MacPherson M., Dow J.A.T., Kaiser K.
    Genetics 154:285-297(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Head.
  5. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
    Strain: Berkeley.
    Tissue: Testis.

Entry informationi

Entry nameiAPH4_DROME
AccessioniPrimary (citable) accession number: Q24238
Secondary accession number(s): B9EQR2
, Q3KN28, Q8IMH0, Q8SXW6, Q9VA19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: October 29, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3