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Q24238

- APH4_DROME

UniProt

Q24238 - APH4_DROME

Protein

Alkaline phosphatase 4

Gene

Aph-4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (28 Mar 2003)
      Previous versions | rss
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    Functioni

    Important role in neural and renal epithelial function.1 Publication

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi93 – 931MagnesiumBy similarity
    Metal bindingi93 – 931Zinc 2By similarity
    Active sitei144 – 1441Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi202 – 2021MagnesiumBy similarity
    Metal bindingi204 – 2041MagnesiumBy similarity
    Metal bindingi369 – 3691MagnesiumBy similarity
    Metal bindingi374 – 3741Zinc 1By similarity
    Metal bindingi378 – 3781Zinc 1By similarity
    Metal bindingi415 – 4151Zinc 2By similarity
    Metal bindingi416 – 4161Zinc 2By similarity
    Metal bindingi504 – 5041Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. epithelial fluid transport Source: UniProtKB
    2. multicellular organism reproduction Source: FlyBase
    3. nervous system development Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase 4 (EC:3.1.3.1)
    Gene namesi
    Name:Aph-4
    ORF Names:CG1462
    OrganismiDrosophila melanogaster (Fruit fly)Imported
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0016123. Aph-4.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: FlyBase
    3. extracellular space Source: FlyBase
    4. integral component of membrane Source: UniProtKB-KW
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 570550Alkaline phosphatase 4PRO_0000024049Add
    BLAST
    Propeptidei571 – 59626Removed in mature formPRO_0000024050Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi539 ↔ 550By similarity
    Lipidationi570 – 5701GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ24238.

    Expressioni

    Tissue specificityi

    Ellipsoid body ring neurons in the adult brain and in the lower Malpighian tubule and ureter.1 Publication

    Developmental stagei

    Highest abundance during larval stage (prior to the secretion of pupal cuticle) and adult stage.1 Publication

    Gene expression databases

    BgeeiQ24238.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ24238.
    SMRiQ24238. Positions 50-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei571 – 59121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1785.
    GeneTreeiENSGT00390000008704.
    HOGENOMiHOG000028185.
    InParanoidiQ24238.
    KOiK01077.
    OMAiWYADANM.
    OrthoDBiEOG7SN8CH.
    PhylomeDBiQ24238.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A1 Publication (identifier: Q24238-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHCLVILGFL LGSLVAFSWA GVTTQPPPLI RTLSAGGDIG PQFDVGKTKE    50
    PEDAEFWHNV GLRQLEKTIK QAQRVKEDSY QKKARNIIIF IGDGMGISTI 100
    SAGRIYKGQY LKHGYGEEET LVFDDFPNTG MAKTYNVDKQ VPDSAGTATA 150
    IFSGSKTHYG AIGMDATRSK KNGQQGRVQS VMEWAQKEGK RTGVVTTTRI 200
    THATPAATYA HIYDRDWECD TEVPAESVGF HVDIARQLVE NAPGNRFNVI 250
    LGGGMSPMGI LNASEVKTTI FEGPTETICT RGDNRNLPAE WLAHHANDTV 300
    PPALVHNRKD LLNVNVKKVD HLMGLFRNNH ITYSIAREAG EPSLQEMTET 350
    ALGILERGDE SNGFVLLVEG GRIDQGHHMN YARAALHELY EFDLAIQAAV 400
    NNTDPDETLI LVTADHSHAV TFNGYALRGA DILGTANSHE KNDPMFYETI 450
    SYANGPGYWD HLANDSRPQN SSNMWMPLKH FTAEERAAPT YRHLATVPRK 500
    DETHGGEDVA VFAYGPGSSL IRGVFEQNYL AYVMSYAGCL GPAKDFDDSC 550
    EDHKDGQKDR PLDKPNPKRN GATVVGASLI PILTAATAAI LRGRGL 596
    Length:596
    Mass (Da):65,262
    Last modified:March 28, 2003 - v3
    Checksum:i333F3345BEFBAEFB
    GO
    Isoform B1 Publication (identifier: Q24238-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-94: Missing.

    Note: No experimental confirmation available.1 Publication

    Show »
    Length:502
    Mass (Da):54,887
    Checksum:iA9E70E72FDAC8AAF
    GO

    Sequence cautioni

    The sequence CAA67052.1 differs from that shown. Reason: Frameshift at position 538.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti200 – 2001I → N in AAL68351. (PubMed:12537569)Curated
    Sequence conflicti280 – 2801T → S in ABA81845. 1 PublicationCurated
    Sequence conflicti358 – 3581G → D in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti375 – 3751Q → H in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti495 – 4962AT → EP in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti570 – 5701N → S in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti573 – 5731T → S in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti593 – 5942GR → CH in CAA67052. (PubMed:10628988)Curated
    Sequence conflicti593 – 5942GR → CH in ABA81845. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9494Missing in isoform B. 2 PublicationsVSP_007002Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98402 mRNA. Translation: CAA67052.1. Frameshift.
    AE014297 Genomic DNA. Translation: AAF57106.1.
    AE014297 Genomic DNA. Translation: AAN14265.1.
    AY075544 mRNA. Translation: AAL68351.1.
    BT023911 mRNA. Translation: ABA81845.1.
    BT057987 mRNA. Translation: ACM16697.1.
    RefSeqiNP_524601.2. NM_079862.3. [Q24238-1]
    NP_733413.1. NM_170534.2. [Q24238-2]
    UniGeneiDm.5439.

    Genome annotation databases

    EnsemblMetazoaiFBtr0085733; FBpp0085095; FBgn0016123. [Q24238-1]
    GeneIDi43671.
    KEGGidme:Dmel_CG1462.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98402 mRNA. Translation: CAA67052.1 . Frameshift.
    AE014297 Genomic DNA. Translation: AAF57106.1 .
    AE014297 Genomic DNA. Translation: AAN14265.1 .
    AY075544 mRNA. Translation: AAL68351.1 .
    BT023911 mRNA. Translation: ABA81845.1 .
    BT057987 mRNA. Translation: ACM16697.1 .
    RefSeqi NP_524601.2. NM_079862.3. [Q24238-1 ]
    NP_733413.1. NM_170534.2. [Q24238-2 ]
    UniGenei Dm.5439.

    3D structure databases

    ProteinModelPortali Q24238.
    SMRi Q24238. Positions 50-545.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q24238.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0085733 ; FBpp0085095 ; FBgn0016123 . [Q24238-1 ]
    GeneIDi 43671.
    KEGGi dme:Dmel_CG1462.

    Organism-specific databases

    CTDi 43671.
    FlyBasei FBgn0016123. Aph-4.

    Phylogenomic databases

    eggNOGi COG1785.
    GeneTreei ENSGT00390000008704.
    HOGENOMi HOG000028185.
    InParanoidi Q24238.
    KOi K01077.
    OMAi WYADANM.
    OrthoDBi EOG7SN8CH.
    PhylomeDBi Q24238.

    Miscellaneous databases

    GenomeRNAii 43671.
    NextBioi 835185.

    Gene expression databases

    Bgeei Q24238.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel Drosophila alkaline phosphatase specific to the ellipsoid body of the adult brain and the lower Malpighian (renal) tubule."
      Yang M.Y., Wang Z., MacPherson M., Dow J.A.T., Kaiser K.
      Genetics 154:285-297(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Head.
    5. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
      Strain: Berkeley.
      Tissue: Testis.

    Entry informationi

    Entry nameiAPH4_DROME
    AccessioniPrimary (citable) accession number: Q24238
    Secondary accession number(s): B9EQR2
    , Q3KN28, Q8IMH0, Q8SXW6, Q9VA19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: March 28, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3