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Protein

Calcineurin subunit B type 2

Gene

CanB2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcineurin is a calcium-binding and calmodulin-binding protein found in all cells from yeast to mammals, and a calcium-dependent, calmodulin-stimulated protein phosphatase.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi31 – 42121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi63 – 74122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi100 – 111123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi141 – 152124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium-dependent protein serine/threonine phosphatase regulator activity Source: FlyBase
  • calcium ion binding Source: FlyBase
  • calmodulin binding Source: FlyBase

GO - Biological processi

  • meiotic nuclear division Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • sarcomere organization Source: FlyBase
  • skeletal muscle myosin thick filament assembly Source: FlyBase
  • sleep Source: FlyBase
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-2871809. FCERI mediated Ca+2 mobilization.
R-DME-4086398. Ca2+ pathway.
R-DME-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 2
Alternative name(s):
Protein phosphatase 2B regulatory subunit 2
dCNB2
Gene namesi
Name:CanB2
Synonyms:CNB2
ORF Names:CG11217
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015614. CanB2.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: FlyBase
  • synaptic vesicle Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 170170Calcineurin subunit B type 2PRO_0000073489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24214.

PTM databases

iPTMnetiQ24214.

Expressioni

Gene expression databases

BgeeiQ24214.
GenevisibleiQ24214. DM.

Interactioni

Subunit structurei

Composed of a catalytic subunit (A) and a regulatory subunit (B) (By similarity). Interacts with sra.By similarity1 Publication

GO - Molecular functioni

  • calmodulin binding Source: FlyBase

Protein-protein interaction databases

BioGridi70250. 2 interactions.
IntActiQ24214. 1 interaction.
STRINGi7227.FBpp0301600.

Structurei

3D structure databases

ProteinModelPortaliQ24214.
SMRiQ24214. Positions 16-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 5134EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini128 – 16336EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
InParanoidiQ24214.
KOiK06268.
OMAiYIGNGEL.
OrthoDBiEOG75QR5D.
PhylomeDBiQ24214.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNETSLPME MCSNFDADEI RRLGKRFRKL DLDNSGALSV DEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDADGNGEV DFKEFIQGVS QFSVKGDKLS KLRFAFRIYD
110 120 130 140 150
MDNDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIGFA DKDEDGKISF
160 170
DEFCSVVGNT DIHKKMVVDV
Length:170
Mass (Da):19,267
Last modified:January 11, 2001 - v2
Checksum:iFDB1BD9DB5A4BDEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56245 Genomic DNA. Translation: AAC47350.1.
AE013599 Genomic DNA. Translation: AAF59195.2.
AY089602 mRNA. Translation: AAL90340.1.
BT003768 mRNA. Translation: AAO41447.1.
PIRiJC5174.
RefSeqiNP_001246192.1. NM_001259263.2.
NP_524874.2. NM_080135.4.
UniGeneiDm.1774.

Genome annotation databases

EnsemblMetazoaiFBtr0088930; FBpp0088004; FBgn0015614.
FBtr0309866; FBpp0301600; FBgn0015614.
GeneIDi46456.
KEGGidme:Dmel_CG11217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56245 Genomic DNA. Translation: AAC47350.1.
AE013599 Genomic DNA. Translation: AAF59195.2.
AY089602 mRNA. Translation: AAL90340.1.
BT003768 mRNA. Translation: AAO41447.1.
PIRiJC5174.
RefSeqiNP_001246192.1. NM_001259263.2.
NP_524874.2. NM_080135.4.
UniGeneiDm.1774.

3D structure databases

ProteinModelPortaliQ24214.
SMRiQ24214. Positions 16-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70250. 2 interactions.
IntActiQ24214. 1 interaction.
STRINGi7227.FBpp0301600.

PTM databases

iPTMnetiQ24214.

Proteomic databases

PaxDbiQ24214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088930; FBpp0088004; FBgn0015614.
FBtr0309866; FBpp0301600; FBgn0015614.
GeneIDi46456.
KEGGidme:Dmel_CG11217.

Organism-specific databases

CTDi46456.
FlyBaseiFBgn0015614. CanB2.

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
InParanoidiQ24214.
KOiK06268.
OMAiYIGNGEL.
OrthoDBiEOG75QR5D.
PhylomeDBiQ24214.

Enzyme and pathway databases

ReactomeiR-DME-2871809. FCERI mediated Ca+2 mobilization.
R-DME-4086398. Ca2+ pathway.
R-DME-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Miscellaneous databases

GenomeRNAii46456.
PROiQ24214.

Gene expression databases

BgeeiQ24214.
GenevisibleiQ24214. DM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila melanogaster contains both X-linked and autosomal homologues of the gene encoding calcineurin B."
    Warren W.D., Phillips A.M., Howells A.J.
    Gene 177:149-153(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Canton-S.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.
  7. "Shaggy/glycogen synthase kinase 3beta and phosphorylation of Sarah/regulator of calcineurin are essential for completion of Drosophila female meiosis."
    Takeo S., Swanson S.K., Nandanan K., Nakai Y., Aigaki T., Washburn M.P., Florens L., Hawley R.S.
    Proc. Natl. Acad. Sci. U.S.A. 109:6382-6389(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRA.

Entry informationi

Entry nameiCANB2_DROME
AccessioniPrimary (citable) accession number: Q24214
Secondary accession number(s): Q9V315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.