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Protein

Eukaryotic translation initiation factor 2 subunit 3

Gene

eIF-2gamma

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548GTPBy similarity
Nucleotide bindingi133 – 1375GTPBy similarity
Nucleotide bindingi189 – 1924GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-382556. ABC-family proteins mediated transport.
R-DME-72649. Translation initiation complex formation.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-DME-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 3
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit gamma
Short name:
eIF-2-gamma
Gene namesi
Name:eIF-2gamma
Synonyms:EIF2-GAMMA, eIF2g
ORF Names:CG6476
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0263740. eIF-2gamma.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Eukaryotic translation initiation factor 2 subunit 3PRO_0000137444Add
BLAST

Proteomic databases

PaxDbiQ24208.
PRIDEiQ24208.

Expressioni

Developmental stagei

Isoform B expressed maternally and zygotically. Isoform B expressed throughout development, in larvae, in pupae and in adult flies.1 Publication

Gene expression databases

BgeeiQ24208.
ExpressionAtlasiQ24208. differential.
GenevisibleiQ24208. DM.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.By similarity

Protein-protein interaction databases

BioGridi66907. 9 interactions.
STRINGi7227.FBpp0302537.

Structurei

3D structure databases

ProteinModelPortaliQ24208.
SMRiQ24208. Positions 38-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 247210tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 548G1PROSITE-ProRule annotation
Regioni75 – 795G2PROSITE-ProRule annotation
Regioni133 – 1364G3PROSITE-ProRule annotation
Regioni189 – 1924G4PROSITE-ProRule annotation
Regioni224 – 2263G5PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0466. Eukaryota.
COG5257. LUCA.
GeneTreeiENSGT00550000074801.
KOiK11419.
OMAiRCTNESC.
OrthoDBiEOG7H1JK8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: Q24208-1) [UniParc]FASTAAdd to basket

Also known as: eIF-2gamma

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAEAQIGV NRNLQKQDLS NLDVSKLTPL SPEVISRQAT INIGTIGHVA
60 70 80 90 100
HGKSTVVKAI SGVQTVRFKN ELERNITIKL GYANAKIYKC DNPKCPRPAS
110 120 130 140 150
FVSDASSKDD SLPCTRLNCS GNFRLVRHVS FVDCPGHDIL MATMLNGAAV
160 170 180 190 200
MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKQILILQNK IDLIKESQAK
210 220 230 240 250
EQYEEITKFV QGTVAEGAPI IPISAQLKYN IDVLCEYIVN KIPVPPRDFN
260 270 280 290 300
APPRLIVIRS FDVNKPGCEV ADLKGGVAGG SILSGVLKVG QEIEVRPGVV
310 320 330 340 350
TKDSDGNITC RPIFSRIVSL FAEQNELQYA VPGGLIGVGT KIDPTLCRAD
360 370 380 390 400
RLVGQVLGAV GQLPDIYQEL EISYYLLRRL LGVRTDGDKK GARVEKLQKN
410 420 430 440 450
EILLVNIGSL STGGRISATK GDLAKIVLTT PVCTEKGEKI ALSRRVENHW
460 470
RLIGWGQIFG GKTITPVLDS QVAKK
Length:475
Mass (Da):51,485
Last modified:November 1, 1996 - v1
Checksum:i8370B07089DD5422
GO
Isoform C (identifier: Q24208-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MATAEAQIGVNRNLQKQDLSNL → MHLRGDVLLGGVAA

Note: No experimental confirmation available.
Show »
Length:467
Mass (Da):50,479
Checksum:i86EED8A178B745D7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222MATAE…DLSNL → MHLRGDVLLGGVAA in isoform C. CuratedVSP_001432Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80069 mRNA. Translation: CAA56375.1.
AJ290956 Genomic DNA. Translation: CAB93767.1.
AE014297 Genomic DNA. Translation: AAF55153.1.
AE014297 Genomic DNA. Translation: AAN13641.1.
AY061102 mRNA. Translation: AAL28650.1.
PIRiS47005. S46941.
RefSeqiNP_001262587.1. NM_001275658.1. [Q24208-1]
NP_731993.1. NM_169629.2. [Q24208-1]
NP_731994.1. NM_169630.2. [Q24208-2]
UniGeneiDm.3299.

Genome annotation databases

EnsemblMetazoaiFBtr0310386; FBpp0302537; FBgn0263740. [Q24208-1]
FBtr0337054; FBpp0307983; FBgn0263740. [Q24208-1]
GeneIDi41843.
KEGGidme:Dmel_CG43665.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80069 mRNA. Translation: CAA56375.1.
AJ290956 Genomic DNA. Translation: CAB93767.1.
AE014297 Genomic DNA. Translation: AAF55153.1.
AE014297 Genomic DNA. Translation: AAN13641.1.
AY061102 mRNA. Translation: AAL28650.1.
PIRiS47005. S46941.
RefSeqiNP_001262587.1. NM_001275658.1. [Q24208-1]
NP_731993.1. NM_169629.2. [Q24208-1]
NP_731994.1. NM_169630.2. [Q24208-2]
UniGeneiDm.3299.

3D structure databases

ProteinModelPortaliQ24208.
SMRiQ24208. Positions 38-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66907. 9 interactions.
STRINGi7227.FBpp0302537.

Proteomic databases

PaxDbiQ24208.
PRIDEiQ24208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0310386; FBpp0302537; FBgn0263740. [Q24208-1]
FBtr0337054; FBpp0307983; FBgn0263740. [Q24208-1]
GeneIDi41843.
KEGGidme:Dmel_CG43665.

Organism-specific databases

CTDi41843.
FlyBaseiFBgn0263740. eIF-2gamma.

Phylogenomic databases

eggNOGiKOG0466. Eukaryota.
COG5257. LUCA.
GeneTreeiENSGT00550000074801.
KOiK11419.
OMAiRCTNESC.
OrthoDBiEOG7H1JK8.

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-382556. ABC-family proteins mediated transport.
R-DME-72649. Translation initiation complex formation.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-DME-72731. Recycling of eIF2:GDP.

Miscellaneous databases

GenomeRNAii41843.
PROiQ24208.

Gene expression databases

BgeeiQ24208.
ExpressionAtlasiQ24208. differential.
GenevisibleiQ24208. DM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The protein encoded by the Drosophila position-effect variegation suppressor gene Su(var)3-9 combines domains of antagonistic regulators of homeotic gene complexes."
    Tschiersch B., Hofmann A., Krauss V., Dorn R., Korge G., Reuter G.
    EMBO J. 13:3822-3831(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
  2. "Two genes become one: the genes encoding heterochromatin protein Su(var)3-9 and translation initiation factor subunit eIF-2gamma are joined to a dicistronic unit in holometabolic insects."
    Krauss V., Reuter G.
    Genetics 156:1157-1167(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Karsnas.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiIF2G_DROME
AccessioniPrimary (citable) accession number: Q24208
Secondary accession number(s): Q9VFA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.