Q24207 (BOULE_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein boule

Gene names

Name:	bol
ORF Names:	CG4760

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA-binding protein that plays a central role in spermatogenesis. Required for meiotic entry and germline differentiation, at the transition between G2 and M phases of meiosis I. Acts by regulating translation of specific mRNAs, possibly by binding to their 3'-UTR. Essential for translation of twine (twe) mRNA. Required for the expression of various genes such as CG6784, CG17210, CG15841 scpr-B, scpr-C, and rho-6. Ref.1 Ref.6 Ref.7
Subcellular location	Nucleus. Cytoplasm. Note: Nuclear in primary spermatocytes until near the end of the meiotic prophase and cytoplasmic localization from then onward. Ref.5
Tissue specificity	Testis specific. Ref.1
Developmental stage	Not expressed in embryos. Expressed in larvae, pupae and adult males. Expressed during spermatogenesis. Ref.1
Sequence similarities	Belongs to the RRM DAZ family. Contains 1 DAZ-like domain. Contains 1 RRM (RNA recognition motif) domain.
Sequence caution	The sequence AAL90061.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Translation regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 21635778. Source: FlyBase lateral inhibition Inferred from mutant phenotype PubMed 19363474. Source: FlyBase male meiosis Inferred from mutant phenotype PubMed 8244010. Source: FlyBase meiotic G2/MI transition Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of translation Inferred from genetic interaction Ref.6. Source: FlyBase spermatocyte division Inferred from mutant phenotype Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: UniProtKB nucleus Inferred from direct assay Ref.5. Source: UniProtKB perikaryon Inferred from direct assay PubMed 18550751. Source: FlyBase
Molecular_function	mRNA binding Inferred from sequence or structural similarity PubMed 10908586. Source: FlyBase nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q24207-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q24207-2)

The sequence of this isoform differs from the canonical sequence as follows:
     189-189: E → G
     190-190: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Protein boule		PRO_0000081497
Regions
	Domain	33 – 110	78	RRM
	Domain	159 – 188	30	DAZ-like
Natural variations
	Alternative sequence	189	1	E → G in isoform 2.		VSP_009450
	Alternative sequence	190	1	Missing in isoform 2.		VSP_009451

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2A5EA1C3223985C3	FASTA	228	24,682
	10 20 30 40 50 60 MHKIAAAPPP SATPGGGLET PLAAPKYGTL IPNRIFVGGI SGDTTEADLT RVFSAYGTVK 70 80 90 100 110 120 STKIIVDRAG VSKGYGFVTF ETEQEAQRLQ ADGECVVLRD RKLNIAPAIK KQPNPLQSIV 130 140 150 160 170 180 ATNGAVYYTT TPPAPISNIP MDQFAAAVYP PAAGVPAIYP PSAMQYQPFY QYYSVPMNVP 190 200 210 220 TIWPQNYQEN HSPLLHSPTS NPHSPHSQSH PQSPCWSIED LRDTLPRV
	Isoform 2 [UniParc]. Checksum: AD9C80E3D64852CA	FASTA	227	24,496
	10 20 30 40 50 60 MHKIAAAPPP SATPGGGLET PLAAPKYGTL IPNRIFVGGI SGDTTEADLT RVFSAYGTVK 70 80 90 100 110 120 STKIIVDRAG VSKGYGFVTF ETEQEAQRLQ ADGECVVLRD RKLNIAPAIK KQPNPLQSIV 130 140 150 160 170 180 ATNGAVYYTT TPPAPISNIP MDQFAAAVYP PAAGVPAIYP PSAMQYQPFY QYYSVPMNVP 190 200 210 220 TIWPQNYQGH SPLLHSPTSN PHSPHSQSHP QSPCWSIEDL RDTLPRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia." Eberhart C.G., Maines J.Z., Wasserman S.A. Nature 381:783-785(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 43-228 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-228 (ISOFORM 2). Strain: Berkeley. Tissue: Testis.
[5]	"Biphasic subcellular localization of the DAZL-related protein boule in Drosophila spermatogenesis." Cheng M.H., Maines J.Z., Wasserman S.A. Dev. Biol. 204:567-576(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule." Maines J.Z., Wasserman S.A. Nat. Cell Biol. 1:171-174(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Human BOULE gene rescues meiotic defects in infertile flies." Xu E.Y., Lee D.F., Klebes A., Turek P.J., Kornberg T.B., Reijo Pera R.A. Hum. Mol. Genet. 12:169-175(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51858 mRNA. Translation: AAC47133.1. AE014296 Genomic DNA. Translation: AAF50316.3. AE014296 Genomic DNA. Translation: AAF50317.3. AI063811 mRNA. No translation available. AY089323 mRNA. Translation: AAL90061.1. Sequence problems.
PIR	S68988.
RefSeq	NP_523989.1. NM_079265.3. NP_729456.1. NM_168318.3. NP_729457.1. NM_168319.2. NP_729458.1. NM_168320.1.
UniGene	Dm.20499.
3D structure databases
ProteinModelPortal	Q24207.
SMR	Q24207. Positions 34-118.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20744N.
IntAct	Q24207. 3 interactions.
MINT	MINT-911886.
Proteomic databases
PaxDb	Q24207.
PRIDE	Q24207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076538; FBpp0076265; FBgn0011206. FBtr0076540; FBpp0076267; FBgn0011206.
GeneID	39049.
KEGG	dme:Dmel_CG4760.
UCSC	CG4760-RA. d. melanogaster.
Organism-specific databases
CTD	39049.
FlyBase	FBgn0011206. bol.
Phylogenomic databases
eggNOG	NOG260394.
GeneTree	ENSGT00530000063480.
InParanoid	Q24207.
OMA	NVPTIWP.
OrthoDB	EOG4S1RQF.
PhylomeDB	Q24207.
Gene expression databases
Bgee	Q24207.
GermOnline	CG4760. Drosophila melanogaster.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	bol. drosophila.
GenomeRNAi	39049.
NextBio	811653.

Entry information

Entry name

BOULE_DROME

Accession

Primary (citable) accession number: Q24207
Secondary accession number(s): Q8T472, Q9VSU4, Q9VSU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project