Reviewed, UniProtKB/Swiss-Prot Q24174 (ABRU_DROME)

Names and origin

Protein names

Recommended name:
    Protein abrupt
Alternative name(s):
    Protein clueless

Gene names

Name:	ab
Synonyms:	clu
ORF Names:	CG4807

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	904 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Expression is vital for development; may be involved in transcriptional regulation. In embryos, muscle specific expression is required for segmental nerve b (SNb) motoneuron target recognition within ventral longitudinal muscles. Has a role in establishing and maintaining embryonic muscle attachments, adult sensory cell formation (macrochaetae) and morphogenesis of adult appendages (legs, antenna aristae and male external genitalia). Has a role in the morphogenesis of the class I dendritic neurons: selective expression of ab in class I da neurons plays a pivotal role in forming dendritic arbors, which are characteristic of the class I cells. The development of more complex arbors of class II-IV neurons depends on the absence of ab. Ref.1 Ref.5
Subcellular location	Nucleus.
Tissue specificity	Expressed in CNS midline cells during embryonic stages 9-13. Expression also seen in cells of the stomagastric nervous system. Segmentally repeated stripes of ectodermal expression appear at stage 11 that become uniform by stage 12 and throughout embryogenesis. Expressed at variable levels in somatic muscles from stage 16 and in all imaginal disks during larval development. Expression is seen in da neurons that grow in two-dimensional dendrites underneath the epidermis during late embryonic, larval, and pupal stages. Ref.1 Ref.5
Disruption phenotype	Flies exhibit disrupts to motoneuron guidance and connectivity. Loss of ab in class I neurons results in malformation of their typical comb-like arbor patterns and generation of supernumerary branch terminals. Ref.1
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axon choice point recognition Traceable author statement. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase lysosome organization Inferred from mutant phenotype. Source: FlyBase muscle attachment Ref.1 Inferred from mutant phenotype. Source: UniProtKB negative regulation of autophagy Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Ref.1 Non-traceable author statement. Source: UniProtKB sensory organ development Ref.1 Inferred from mutant phenotype. Source: UniProtKB synaptic target recognition Ref.1 Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro transcription factor activity Ref.1 Non-traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q24174-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q24174-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     356-365: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 904	904	Protein abrupt		PRO_0000046907
Regions
	Domain	103 – 168	66	BTB
	Zinc finger	544 – 567	24	C2H2-type 1
	Zinc finger	573 – 596	24	C2H2-type 2
	Compositional bias	196 – 369	174	Ala/Asn/Ser-rich
Amino acid modifications
	Modified residue	474	1	Phosphoserine Ref.6
	Modified residue	837	1	Phosphoserine Ref.6
	Modified residue	846	1	Phosphoserine Ref.6
	Modified residue	868	1	Phosphoserine Ref.6
	Modified residue	889	1	Phosphoserine Ref.6
	Modified residue	896	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	356 – 365	10	Missing in isoform B. Ref.1		VSP_006823
Experimental info
	Mutagenesis	585	1	R → C in allele AB-CLU2; lethal. Ref.1
	Sequence conflict	264	1	H → P in AAN71338. Ref.4
	Sequence conflict	695	1	S → T in AAA86639. Ref.1
	Sequence conflict	888	1	N → D in AAN71338. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (Long) [UniParc]. Last modified October 1, 2001. Version 2. Checksum: 9DF8F4EDDF8E5CF7	FASTA	904	95,093
	10 20 30 40 50 60 MTESTQLQTA ENNNAGVVKM EPPPPATSSV SVSAAAAAHA LSSLSSLTMA ATGSALSPAT 70 80 90 100 110 120 PPPSLNLSHQ QQQHQQHYAL KWNDFQSSIL SSFRHLRDEE DFVDVTLACD ERSFTAHKVV 130 140 150 160 170 180 LSACSPYFRR LLKANPCEHP IVILRDVRCD DVENLLSFMY NGEVNVSHEQ LPDFLKTAHL 190 200 210 220 230 240 LQIRGLADVN GGYPYSKALS AALSHNSSNN NNNNSSSNNS LSNNNNNNNN NAESSNHNKI 250 260 270 280 290 300 SSYLSPNQTS AACNNSSNSN SNNHSSSHNN SSSNNISGSL NSSLNSPFSA PQIPPPVTAS 310 320 330 340 350 360 SAAAAAAAAA SLTAAVAAAA AATAASAGSS SSAASGQTSG TPAIQELKAS SAASPVRNPN 370 380 390 400 410 420 PNPSKASSSN HWDMGEMEGS RKSHLTPPPQ KRIKSADLFR AQHGISPERL LLDREFPVAG 430 440 450 460 470 480 QHPLTRNRSG RDTSKDRERN LELRESLLGQ ALENSNGQQA NPKHELGQSA GEDSNSSDTE 490 500 510 520 530 540 PSDRGDGQHD GTLDGIDNQR SHSFPNAFLG LQGIPGLLPG PSGINSDFVS RRSLEMRVRA 550 560 570 580 590 600 TDPRPCPKCG KIYRSAHTLR THLEDKHTVC PGYRCVLCGT VAKSRNSLHS HMSRQHRGIS 610 620 630 640 650 660 TKDLPVLPMP SAFDPELASR LLAKAGVKIS PAELRARASP TGGSGSSGGG GGGGSSQAKL 670 680 690 700 710 720 DLSNASGGPM DDAEDSDDDP EDLTTGNGLY GMGGSSSDLS RYHESLLSNF GHARMRNEAA 730 740 750 760 770 780 AVAATAAALG QPKDLGVQLP NSNAPGQSLL DTYLQFITEN TFGMGMSQEQ AAAAALRAKM 790 800 810 820 830 840 AQLNAMGHSL DNLPPGLLPG QFDLSKLAAG NPAFGQSGPG LTIEPIMRHE QAAGNLSPNR 850 860 870 880 890 900 PLALNSGGRM MGHDEMAEND GDMRREGSEP MDLGLDNNQS GSNHEVANSD AEENYSEDEG VHNT
	Isoform B (Short). Checksum: 2124D0784FA03228	FASTA	894	93,989
	10 20 30 40 50 60 MTESTQLQTA ENNNAGVVKM EPPPPATSSV SVSAAAAAHA LSSLSSLTMA ATGSALSPAT 70 80 90 100 110 120 PPPSLNLSHQ QQQHQQHYAL KWNDFQSSIL SSFRHLRDEE DFVDVTLACD ERSFTAHKVV 130 140 150 160 170 180 LSACSPYFRR LLKANPCEHP IVILRDVRCD DVENLLSFMY NGEVNVSHEQ LPDFLKTAHL 190 200 210 220 230 240 LQIRGLADVN GGYPYSKALS AALSHNSSNN NNNNSSSNNS LSNNNNNNNN NAESSNHNKI 250 260 270 280 290 300 SSYLSPNQTS AACNNSSNSN SNNHSSSHNN SSSNNISGSL NSSLNSPFSA PQIPPPVTAS 310 320 330 340 350 360 SAAAAAAAAA SLTAAVAAAA AATAASAGSS SSAASGQTSG TPAIQELKAS SAASPASSSN 370 380 390 400 410 420 HWDMGEMEGS RKSHLTPPPQ KRIKSADLFR AQHGISPERL LLDREFPVAG QHPLTRNRSG 430 440 450 460 470 480 RDTSKDRERN LELRESLLGQ ALENSNGQQA NPKHELGQSA GEDSNSSDTE PSDRGDGQHD 490 500 510 520 530 540 GTLDGIDNQR SHSFPNAFLG LQGIPGLLPG PSGINSDFVS RRSLEMRVRA TDPRPCPKCG 550 560 570 580 590 600 KIYRSAHTLR THLEDKHTVC PGYRCVLCGT VAKSRNSLHS HMSRQHRGIS TKDLPVLPMP 610 620 630 640 650 660 SAFDPELASR LLAKAGVKIS PAELRARASP TGGSGSSGGG GGGGSSQAKL DLSNASGGPM 670 680 690 700 710 720 DDAEDSDDDP EDLTTGNGLY GMGGSSSDLS RYHESLLSNF GHARMRNEAA AVAATAAALG 730 740 750 760 770 780 QPKDLGVQLP NSNAPGQSLL DTYLQFITEN TFGMGMSQEQ AAAAALRAKM AQLNAMGHSL 790 800 810 820 830 840 DNLPPGLLPG QFDLSKLAAG NPAFGQSGPG LTIEPIMRHE QAAGNLSPNR PLALNSGGRM 850 860 870 880 890 MGHDEMAEND GDMRREGSEP MDLGLDNNQS GSNHEVANSD AEENYSEDEG VHNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila abrupt gene encodes a BTB-zinc finger regulatory protein that controls the specificity of neuromuscular connections." Hu S., Fambrough D., Atashi J.R., Goodman C.S., Crews S.T. Genes Dev. 9:2936-2948(1995) [PubMed: 7498790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-585, DISRUPTION PHENOTYPE. Strain: Canton-S. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
[5]	"Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt." Sugimura K., Satoh D., Estes P., Crews S., Uemura T. Neuron 43:809-822(2004) [PubMed: 15363392] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-837; SER-846; SER-868; SER-889 AND SER-896, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43733 mRNA. Translation: AAA86639.1. AE014134 Genomic DNA. Translation: AAF53087.2. AE014134 Genomic DNA. Translation: AAN10774.1. BT001583 mRNA. Translation: AAN71338.1.
RefSeq	NP_476562.1. NP_476563.1.
UniGene	Dm.1729
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19247N.
IntAct	Q24174. 5 interactions.
STRING	Q24174.
Genome annotation databases
Ensembl	FBtr0080203; FBpp0079792; FBgn0259750; Drosophila melanogaster. [Genome view] FBtr0080204; FBpp0079793; FBgn0259750; Drosophila melanogaster. [Genome view] FBtr0300472; FBpp0289699; FBgn0259750; Drosophila melanogaster. [Genome view] FBtr0300473; FBpp0289700; FBgn0259750; Drosophila melanogaster. [Genome view]
GeneID	34560.
KEGG	dme:Dmel_CG4807.
UCSC	CG4807-RA. d. melanogaster.
Organism-specific databases
CTD	34560.
FlyBase	FBgn0259750. ab.
Phylogenomic databases
OMA	REESHAF
OrthoDB	EOG9894TH
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-008543-MON.
Gene expression databases
GermOnline	CG4807. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	789065.

Entry information

Entry name

ABRU_DROME

Accession

Primary (citable) accession number: Q24174
Secondary accession number(s): Q9VKI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	October 1, 2001
Last modified:	November 24, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project