Q24167 (SIMA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein similar

Gene names

Name:	sima
ORF Names:	CG7951

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1507 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possible DNA-binding transcriptional activator.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Interacts with VHL.
Subcellular location	Nucleus Potential.
Tissue specificity	Ubiquitously expressed in the embryo.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil Repeat
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to hypoxia Inferred from direct assay PubMed 12215541PubMed 16278294. Source: FlyBase cellular response to insulin stimulus Inferred from direct assay PubMed 16278294. Source: FlyBase negative regulation of cell growth Inferred from mutant phenotype PubMed 16179946. Source: FlyBase positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 12215541. Source: FlyBase response to DNA damage stimulus Inferred from mutant phenotype PubMed 19543366. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 12215541. Source: FlyBase nucleus Inferred from direct assay PubMed 12215541. Source: FlyBase
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1507	1507	Protein similar		PRO_0000127444
Regions
	Domain	72 – 125	54	bHLH
	Domain	167 – 240	74	PAS 1
	Domain	307 – 377	71	PAS 2
	Domain	381 – 422	42	PAC
	Coiled coil	880 – 908	29	Potential
	Coiled coil	982 – 1054	73	Potential
	Coiled coil	1110 – 1162	53	Potential
	Compositional bias	26 – 39	14	Poly-Ser
	Compositional bias	577 – 587	11	Pro-rich
	Compositional bias	718 – 725	8	Poly-Ser
	Compositional bias	759 – 763	5	Poly-Gln
	Compositional bias	767 – 776	10	Poly-Gln
	Compositional bias	907 – 918	12	Poly-Gln
	Compositional bias	945 – 948	4	Poly-Gln
	Compositional bias	990 – 998	9	Poly-Gln
	Compositional bias	1020 – 1038	19	Poly-Gln
	Compositional bias	1113 – 1126	14	Poly-Gln
	Compositional bias	1146 – 1162	17	Poly-Gln
	Compositional bias	1205 – 1208	4	Poly-Gln
	Compositional bias	1277 – 1284	8	Poly-Gln
	Compositional bias	1298 – 1301	4	Poly-Asp
Experimental info
	Sequence conflict	38	1	S → A in AAC47303. Ref.1
	Sequence conflict	345	1	S → L in AAC47303. Ref.1
	Sequence conflict	492	1	A → V in AAC47303. Ref.1
	Sequence conflict	588	1	T → I in AAC47303. Ref.1
	Sequence conflict	709	1	T → K in AAC47303. Ref.1
	Sequence conflict	776	1	Q → QQQQ Ref.1
	Sequence conflict	895	1	Q → QQ in AAC47303. Ref.1
	Sequence conflict	902	1	G → S in AAC47303. Ref.1
	Sequence conflict	982	1	A → T in AAC47303. Ref.1
	Sequence conflict	1125 – 1126	2	Missing Ref.1
	Sequence conflict	1154 – 1157	4	Missing Ref.1
	Sequence conflict	1444	1	F → L in AAC47303. Ref.1
	Sequence conflict	1447	1	G → C in AAC47303. Ref.1
	Sequence conflict	1451	1	S → N in AAC47303. Ref.1
	Sequence conflict	1494	1	D → G in AAC47303. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q24167 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: 4102939C8FBFB0C6	FASTA	1,507	165,824
	10 20 30 40 50 60 MVSLIDTIEA AAEKQKQSQA VVTNTSASSS SCSSSFSSSP PSSSVGSPSP GAPKTNLTAS 70 80 90 100 110 120 GKPKEKRRNN EKRKEKSRDA ARCRRSKETE IFMELSAALP LKTDDVNQLD KASVMRITIA 130 140 150 160 170 180 FLKIREMLQF VPSLRDCNDD IKQDIETAED QQEVKPKLEV GTEDWLNGAE ARELLKQTMD 190 200 210 220 230 240 GFLLVLSHEG DITYVSENVV EYLGITKIDT LGQQIWEYSH QCDHAEIKEA LSLKRELAQK 250 260 270 280 290 300 VKDEPQQNSG VSTHHRDLFV RLKCTLTSRG RSINIKSASY KVIHITGHLV VNAKGERLLM 310 320 330 340 350 360 AIGRPIPHPS NIEIPLGTST FLTKHSLDMR FTYVDDKMHD LLGYSPKDLL DTSLFSCQHG 370 380 390 400 410 420 ADSERLMATF KSVLSKGQGE TSRYRFLGKY GGYCWILSQA TIVYDKLKPQ SVVCVNYVIS 430 440 450 460 470 480 NLENKHEIYS LAQQTAASEQ KEQHHQAAET EKEPEKAADP EIIAQETKET VNTPIHTSEL 490 500 510 520 530 540 QAKPLQLESE KAEKTIEETK TIATIPPVTA TSTADQIKQL PESNPYKQIL QAELLIKREN 550 560 570 580 590 600 HSPGPRTITA QLLSGSSSGL RPEEKRPKSV TASVLRPSPA PPLTPPPTAV LCKKTPLGVE 610 620 630 640 650 660 PNLPPTTTAT AAIISSSNQQ LQIAQQTQLQ NPQQPAQDMS KGFCSLFADD GRGLTMLKEE 670 680 690 700 710 720 PDDLSHHLAS TNCIQLDEMT PFSDMLVGLM GTCLLPEDIN SLDSTTCSTT ASGQHYQSPS 730 740 750 760 770 780 SSSTSAPSNT SSSNNSYANS PLSPLTPNST ATASNPSHQQ QQQHHNQQQQ QQQQQQHHPQ 790 800 810 820 830 840 HHDNSNSSSN IDPLFNYREE SNDTSCSQHL HSPSITSKSP EDSSLPSLCS PNSLTQEDDF 850 860 870 880 890 900 SFEAFAMRAP YIPIDDDMPL LTETDLMWCP PEDLQTMVPK EIDAIQQQLQ QLQQQHHQQY 910 920 930 940 950 960 AGNTGYQQQQ QQPQLQQQHF SNSLCSSPAS TVSSLSPSPV QQHHQQQQAA VFTSDSSELA 970 980 990 1000 1010 1020 ALLCGSGNGT LSILAGSGVT VAEECNERLQ QHQQQQQQTS GNEFRTFQQL QQELQLQEEQ 1030 1040 1050 1060 1070 1080 QQRQQQQQQQ QQQQQQQQLL SLNIECKKEK YDVQMGGSLC HPMEDAFEND YSKDSANLDC 1090 1100 1110 1120 1130 1140 WDLIQMQVVD TEPVSPNAAS PTPCKVSAIQ LLQQQQQLQQ QQQQQQNIIL NAVPLITIQN 1150 1160 1170 1180 1190 1200 NKELMQQQQQ QQQQQQQEQL QQPAIKLLNG ASIAPVNTKA TIRLVESKPP TTTQSRMAKV 1210 1220 1230 1240 1250 1260 NLVPQQQQHG NKRHLNSATG AGNPVESKRL KSGTLCLDVQ SPQLLQQLIG KDPAQQQTQA 1270 1280 1290 1300 1310 1320 AKRAGSERWQ LSAESKQQKQ QQQQSNSVLK NLLVSGRDDD DSEAMIIDED NSLVQPIPLG 1330 1340 1350 1360 1370 1380 KYGLPLHCHT STSSVLRDYH NNPLISGTNF QLSPVFGGSD SSGGDGETGS VVSLDDSVPP 1390 1400 1410 1420 1430 1440 GLTACDTDAS SDSGIDENSL MDGASGSPRK RLSSTSNSTN QAESAPPALD VETPVTQKSV 1450 1460 1470 1480 1490 1500 EEEFEGGGSG SNAPSRKTSI SFLDSSNPLL HTPAMMDLVN DDYIMGEGGF EFSDNQLEQV LGWPEIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila melanogaster similar bHLH-PAS gene encodes a protein related to human hypoxia-inducible factor 1 alpha and Drosophila single-minded." Nambu J.R., Chen W., Hu S., Crews S.T. Gene 172:249-254(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43090 mRNA. Translation: AAC47303.1. AE014297 Genomic DNA. Translation: AAF57008.2.
PIR	JC4851.
RefSeq	NP_524584.2. NM_079845.4.
UniGene	Dm.6953.
3D structure databases
ProteinModelPortal	Q24167.
SMR	Q24167. Positions 74-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-21002N.
IntAct	Q24167. 3 interactions.
MINT	MINT-304504.
STRING	7227.FBpp0084931.
Proteomic databases
PaxDb	Q24167.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085565; FBpp0084931; FBgn0015542.
GeneID	43580.
KEGG	dme:Dmel_CG7951.
Organism-specific databases
CTD	43580.
FlyBase	FBgn0015542. sima.
Phylogenomic databases
eggNOG	NOG289264.
GeneTree	ENSGT00650000093281.
InParanoid	Q24167.
KO	K08268.
OMA	PGPRTIT.
OrthoDB	EOG4JQ2CF.
PhylomeDB	Q24167.
Gene expression databases
Bgee	Q24167.
GermOnline	CG7951. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011598. bHLH_dom. IPR001610. PAC. IPR000014. PAS. IPR013767. PAS_fold. IPR013655. PAS_fold_3. [Graphical view]
Pfam	PF00989. PAS. 1 hit. PF08447. PAS_3. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 2 hits. [Graphical view]
PROSITE	PS50888. BHLH. 1 hit. PS50113. PAC. False negative. PS50112. PAS. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	sima. drosophila.
GenomeRNAi	43580.
NextBio	834672.

Entry information

Entry name

SIMA_DROME

Accession

Primary (citable) accession number: Q24167
Secondary accession number(s): Q9VAA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 21, 2001
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project