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Protein

Protein similar

Gene

sima

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional regulator of the adaptive response to hypoxia. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • cellular response to hypoxia Source: FlyBase
  • cellular response to insulin stimulus Source: FlyBase
  • negative regulation of cell growth Source: FlyBase
  • oogenesis Source: FlyBase
  • positive regulation of autophagy Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of cell cycle Source: FlyBase
  • regulation of cell migration Source: FlyBase
  • regulation of innate immune response Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • trachea development Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-1234158. Regulation of gene expression by Hypoxia-inducible Factor.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-8951664. Neddylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein similar
Gene namesi
Name:sima
ORF Names:CG45051
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266411. sima.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nucleus Source: FlyBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274441 – 1507Protein similarAdd BLAST1507

Proteomic databases

PaxDbiQ24167.
PRIDEiQ24167.

Expressioni

Tissue specificityi

Ubiquitously expressed in the embryo.1 Publication

Inductioni

By hypoxia.2 Publications

Gene expression databases

BgeeiFBgn0015542.
ExpressionAtlasiQ24167. differential.
GenevisibleiQ24167. DM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Interacts with Vhl.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68436. 7 interactors.
DIPiDIP-21002N.
IntActiQ24167. 4 interactors.
MINTiMINT-304504.
STRINGi7227.FBpp0084931.

Structurei

3D structure databases

ProteinModelPortaliQ24167.
SMRiQ24167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 125bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini167 – 240PAS 1PROSITE-ProRule annotationAdd BLAST74
Domaini307 – 377PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini381 – 422PACAdd BLAST42

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni692 – 863ODDAdd BLAST172

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili880 – 908Sequence analysisAdd BLAST29
Coiled coili982 – 1054Sequence analysisAdd BLAST73
Coiled coili1110 – 1162Sequence analysisAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 39Poly-SerAdd BLAST14
Compositional biasi577 – 587Pro-richAdd BLAST11
Compositional biasi718 – 725Poly-Ser8
Compositional biasi759 – 763Poly-Gln5
Compositional biasi767 – 776Poly-Gln10
Compositional biasi907 – 918Poly-GlnAdd BLAST12
Compositional biasi945 – 948Poly-Gln4
Compositional biasi990 – 998Poly-Gln9
Compositional biasi1020 – 1038Poly-GlnAdd BLAST19
Compositional biasi1113 – 1126Poly-GlnAdd BLAST14
Compositional biasi1146 – 1162Poly-GlnAdd BLAST17
Compositional biasi1205 – 1208Poly-Gln4
Compositional biasi1277 – 1284Poly-Gln8
Compositional biasi1298 – 1301Poly-Asp4

Domaini

The oxygen-dependent degradation (ODD) domain is required for cytoplasmic localization in normoxia.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3558. Eukaryota.
ENOG410YK57. LUCA.
InParanoidiQ24167.
KOiK08268.
OrthoDBiEOG091G0486.
PhylomeDBiQ24167.

Family and domain databases

CDDicd00083. HLH. 1 hit.
cd00130. PAS. 2 hits.
Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
IPR015943. WD40/YVTN_repeat-like_dom.
PfamiView protein in Pfam
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.

Sequencei

Sequence statusi: Complete.

Q24167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLIDTIEA AAEKQKQSQA VVTNTSASSS SCSSSFSSSP PSSSVGSPSP
60 70 80 90 100
GAPKTNLTAS GKPKEKRRNN EKRKEKSRDA ARCRRSKETE IFMELSAALP
110 120 130 140 150
LKTDDVNQLD KASVMRITIA FLKIREMLQF VPSLRDCNDD IKQDIETAED
160 170 180 190 200
QQEVKPKLEV GTEDWLNGAE ARELLKQTMD GFLLVLSHEG DITYVSENVV
210 220 230 240 250
EYLGITKIDT LGQQIWEYSH QCDHAEIKEA LSLKRELAQK VKDEPQQNSG
260 270 280 290 300
VSTHHRDLFV RLKCTLTSRG RSINIKSASY KVIHITGHLV VNAKGERLLM
310 320 330 340 350
AIGRPIPHPS NIEIPLGTST FLTKHSLDMR FTYVDDKMHD LLGYSPKDLL
360 370 380 390 400
DTSLFSCQHG ADSERLMATF KSVLSKGQGE TSRYRFLGKY GGYCWILSQA
410 420 430 440 450
TIVYDKLKPQ SVVCVNYVIS NLENKHEIYS LAQQTAASEQ KEQHHQAAET
460 470 480 490 500
EKEPEKAADP EIIAQETKET VNTPIHTSEL QAKPLQLESE KAEKTIEETK
510 520 530 540 550
TIATIPPVTA TSTADQIKQL PESNPYKQIL QAELLIKREN HSPGPRTITA
560 570 580 590 600
QLLSGSSSGL RPEEKRPKSV TASVLRPSPA PPLTPPPTAV LCKKTPLGVE
610 620 630 640 650
PNLPPTTTAT AAIISSSNQQ LQIAQQTQLQ NPQQPAQDMS KGFCSLFADD
660 670 680 690 700
GRGLTMLKEE PDDLSHHLAS TNCIQLDEMT PFSDMLVGLM GTCLLPEDIN
710 720 730 740 750
SLDSTTCSTT ASGQHYQSPS SSSTSAPSNT SSSNNSYANS PLSPLTPNST
760 770 780 790 800
ATASNPSHQQ QQQHHNQQQQ QQQQQQHHPQ HHDNSNSSSN IDPLFNYREE
810 820 830 840 850
SNDTSCSQHL HSPSITSKSP EDSSLPSLCS PNSLTQEDDF SFEAFAMRAP
860 870 880 890 900
YIPIDDDMPL LTETDLMWCP PEDLQTMVPK EIDAIQQQLQ QLQQQHHQQY
910 920 930 940 950
AGNTGYQQQQ QQPQLQQQHF SNSLCSSPAS TVSSLSPSPV QQHHQQQQAA
960 970 980 990 1000
VFTSDSSELA ALLCGSGNGT LSILAGSGVT VAEECNERLQ QHQQQQQQTS
1010 1020 1030 1040 1050
GNEFRTFQQL QQELQLQEEQ QQRQQQQQQQ QQQQQQQQLL SLNIECKKEK
1060 1070 1080 1090 1100
YDVQMGGSLC HPMEDAFEND YSKDSANLDC WDLIQMQVVD TEPVSPNAAS
1110 1120 1130 1140 1150
PTPCKVSAIQ LLQQQQQLQQ QQQQQQNIIL NAVPLITIQN NKELMQQQQQ
1160 1170 1180 1190 1200
QQQQQQQEQL QQPAIKLLNG ASIAPVNTKA TIRLVESKPP TTTQSRMAKV
1210 1220 1230 1240 1250
NLVPQQQQHG NKRHLNSATG AGNPVESKRL KSGTLCLDVQ SPQLLQQLIG
1260 1270 1280 1290 1300
KDPAQQQTQA AKRAGSERWQ LSAESKQQKQ QQQQSNSVLK NLLVSGRDDD
1310 1320 1330 1340 1350
DSEAMIIDED NSLVQPIPLG KYGLPLHCHT STSSVLRDYH NNPLISGTNF
1360 1370 1380 1390 1400
QLSPVFGGSD SSGGDGETGS VVSLDDSVPP GLTACDTDAS SDSGIDENSL
1410 1420 1430 1440 1450
MDGASGSPRK RLSSTSNSTN QAESAPPALD VETPVTQKSV EEEFEGGGSG
1460 1470 1480 1490 1500
SNAPSRKTSI SFLDSSNPLL HTPAMMDLVN DDYIMGEGGF EFSDNQLEQV

LGWPEIA
Length:1,507
Mass (Da):165,824
Last modified:February 21, 2001 - v2
Checksum:i4102939C8FBFB0C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38S → A in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti345S → L in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti492A → V in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti588T → I in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti709T → K in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti776Q → QQQQ in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti895Q → QQ in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti902G → S in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti982A → T in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti1125 – 1126Missing in AAC47303 (PubMed:8682312).Curated2
Sequence conflicti1154 – 1157Missing in AAC47303 (PubMed:8682312).Curated4
Sequence conflicti1444F → L in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti1447G → C in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti1451S → N in AAC47303 (PubMed:8682312).Curated1
Sequence conflicti1494D → G in AAC47303 (PubMed:8682312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43090 mRNA. Translation: AAC47303.1.
AE014297 Genomic DNA. Translation: AAF57008.2.
PIRiJC4851.
RefSeqiNP_524584.2. NM_079845.4.
UniGeneiDm.6953.

Genome annotation databases

EnsemblMetazoaiFBtr0344374; FBpp0310747; FBgn0266411.
GeneIDi43580.
KEGGidme:Dmel_CG45051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43090 mRNA. Translation: AAC47303.1.
AE014297 Genomic DNA. Translation: AAF57008.2.
PIRiJC4851.
RefSeqiNP_524584.2. NM_079845.4.
UniGeneiDm.6953.

3D structure databases

ProteinModelPortaliQ24167.
SMRiQ24167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68436. 7 interactors.
DIPiDIP-21002N.
IntActiQ24167. 4 interactors.
MINTiMINT-304504.
STRINGi7227.FBpp0084931.

Proteomic databases

PaxDbiQ24167.
PRIDEiQ24167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0344374; FBpp0310747; FBgn0266411.
GeneIDi43580.
KEGGidme:Dmel_CG45051.

Organism-specific databases

CTDi43580.
FlyBaseiFBgn0266411. sima.

Phylogenomic databases

eggNOGiKOG3558. Eukaryota.
ENOG410YK57. LUCA.
InParanoidiQ24167.
KOiK08268.
OrthoDBiEOG091G0486.
PhylomeDBiQ24167.

Enzyme and pathway databases

ReactomeiR-DME-1234158. Regulation of gene expression by Hypoxia-inducible Factor.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-8951664. Neddylation.

Miscellaneous databases

ChiTaRSisima. fly.
GenomeRNAii43580.
PROiPR:Q24167.

Gene expression databases

BgeeiFBgn0015542.
ExpressionAtlasiQ24167. differential.
GenevisibleiQ24167. DM.

Family and domain databases

CDDicd00083. HLH. 1 hit.
cd00130. PAS. 2 hits.
Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
IPR015943. WD40/YVTN_repeat-like_dom.
PfamiView protein in Pfam
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSIMA_DROME
AccessioniPrimary (citable) accession number: Q24167
Secondary accession number(s): Q9VAA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: June 7, 2017
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.