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Protein

Beta-1,3-galactosyltransferase brn

Gene

brn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Neurogenic protein essential for the development and maintenance of epithelial structure. Required in the germline for establishing the follicular epithelium and for determining the dorsal-ventral polarity. Collaborates with Notch on the apical surface of follicle cells to mediate germline-follicle cell adhesion. Brn has a role in chorion formation.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + an N-acetyl-beta-D-galactosaminyl-(1->4)-(alpha-N-acetylneuraminyl-(2->3))-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-(alpha-N-acetylneuraminyl-(2->3))-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

GO - Molecular functioni

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • glycosphingolipid biosynthetic process Source: FlyBase
  • maintenance of polarity of follicular epithelium Source: FlyBase
  • morphogenesis of follicular epithelium Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • Notch signaling pathway Source: UniProtKB-KW
  • oogenesis Source: FlyBase
  • optic lobe placode formation Source: FlyBase
  • ovarian follicle cell-cell adhesion Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • protein glycosylation Source: InterPro

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
Biological processNotch signaling pathway

Enzyme and pathway databases

BRENDAi2.4.1.62 1994
SignaLinkiQ24157

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyltransferase brn (EC:2.4.1.62)
Alternative name(s):
Brainiac protein
Neurogenic secreted-signaling protein brn
Gene namesi
Name:brn
ORF Names:CG4934
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000221 brn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 325LumenalSequence analysisAdd BLAST297

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191741 – 325Beta-1,3-galactosyltransferase brnAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ24157
PRIDEiQ24157

Expressioni

Gene expression databases

BgeeiFBgn0000221
GenevisibleiQ24157 DM

Interactioni

Protein-protein interaction databases

BioGridi578762 interactors.
IntActiQ24157 2 interactors.
STRINGi7227.FBpp0070606

Structurei

3D structure databases

ProteinModelPortaliQ24157
SMRiQ24157
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00900000141150
InParanoidiQ24157
KOiK02175
OMAiIRRTWGY
OrthoDBiEOG091G0BM6
PhylomeDBiQ24157

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q24157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSKHRKLLL RCLLVLPLIL LVDYCGLLTH LHELNFERHF HYPLNDDTGS
60 70 80 90 100
GSASSGLDKF AYLRVPSFTA EVPVDQPARL TMLIKSAVGN SRRREAIRRT
110 120 130 140 150
WGYEGRFSDV HLRRVFLLGT AEDSEKDVAW ESREHGDILQ AEFTDAYFNN
160 170 180 190 200
TLKTMLGMRW ASDQFNRSEF YLFVDDDYYV SAKNVLKFLG RGRQSHQPEL
210 220 230 240 250
LFAGHVFQTS PLRHKFSKWY VSLEEYPFDR WPPYVTAGAF ILSQKALRQL
260 270 280 290 300
YAASVHLPLF RFDDVYLGIV ALKAGISLQH CDDFRFHRPA YKGPDSYSSV
310 320
IASHEFGDPE EMTRVWNECR SANYA
Length:325
Mass (Da):37,620
Last modified:May 10, 2004 - v2
Checksum:i0DF89B720F43657B
GO

Sequence cautioni

The sequence AAL48007 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142E → D in AAA85211 (PubMed:9012507).Curated1
Sequence conflicti163D → E in AAA85211 (PubMed:9012507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41449 mRNA Translation: AAA85211.1
AE014298 Genomic DNA Translation: AAF45918.1
AL033125 Genomic DNA Translation: CAA21833.1
BT021250 mRNA Translation: AAX33398.1
AY070536 mRNA Translation: AAL48007.1 Different initiation.
RefSeqiNP_476901.1, NM_057553.5
UniGeneiDm.1589

Genome annotation databases

EnsemblMetazoaiFBtr0070638; FBpp0070606; FBgn0000221
GeneIDi31358
KEGGidme:Dmel_CG4934

Similar proteinsi

Entry informationi

Entry nameiBRN_DROME
AccessioniPrimary (citable) accession number: Q24157
Secondary accession number(s): Q5BIH4, Q8SZS9, Q9W4N0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: February 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome