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Protein

Beta-1,3-galactosyltransferase brn

Gene

brn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Neurogenic protein essential for the development and maintenance of epithelial structure. Required in the germline for establishing the follicular epithelium and for determining the dorsal-ventral polarity. Collaborates with Notch on the apical surface of follicle cells to mediate germline-follicle cell adhesion. Brn has a role in chorion formation.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + an N-acetyl-beta-D-galactosaminyl-(1->4)-(alpha-N-acetylneuraminyl-(2->3))-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-(alpha-N-acetylneuraminyl-(2->3))-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

GO - Molecular functioni

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • glycosphingolipid biosynthetic process Source: FlyBase
  • maintenance of polarity of follicular epithelium Source: FlyBase
  • morphogenesis of follicular epithelium Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • Notch signaling pathway Source: UniProtKB-KW
  • oogenesis Source: FlyBase
  • optic lobe placode formation Source: FlyBase
  • ovarian follicle cell-cell adhesion Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • protein glycosylation Source: InterPro

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
Biological processNotch signaling pathway

Enzyme and pathway databases

BRENDAi2.4.1.62. 1994.
SignaLinkiQ24157.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyltransferase brn (EC:2.4.1.62)
Alternative name(s):
Brainiac protein
Neurogenic secreted-signaling protein brn
Gene namesi
Name:brn
ORF Names:CG4934
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000221. brn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 325LumenalSequence analysisAdd BLAST297

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191741 – 325Beta-1,3-galactosyltransferase brnAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ24157.
PRIDEiQ24157.

Expressioni

Gene expression databases

BgeeiFBgn0000221.
GenevisibleiQ24157. DM.

Interactioni

Protein-protein interaction databases

BioGridi57876. 2 interactors.
IntActiQ24157. 2 interactors.
MINTiMINT-283889.
STRINGi7227.FBpp0070606.

Structurei

3D structure databases

ProteinModelPortaliQ24157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00880000138012.
InParanoidiQ24157.
KOiK02175.
OMAiIRRTWGY.
OrthoDBiEOG091G0BM6.
PhylomeDBiQ24157.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR002659. Glyco_trans_31.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiView protein in Pfam
PF01762. Galactosyl_T. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q24157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSKHRKLLL RCLLVLPLIL LVDYCGLLTH LHELNFERHF HYPLNDDTGS
60 70 80 90 100
GSASSGLDKF AYLRVPSFTA EVPVDQPARL TMLIKSAVGN SRRREAIRRT
110 120 130 140 150
WGYEGRFSDV HLRRVFLLGT AEDSEKDVAW ESREHGDILQ AEFTDAYFNN
160 170 180 190 200
TLKTMLGMRW ASDQFNRSEF YLFVDDDYYV SAKNVLKFLG RGRQSHQPEL
210 220 230 240 250
LFAGHVFQTS PLRHKFSKWY VSLEEYPFDR WPPYVTAGAF ILSQKALRQL
260 270 280 290 300
YAASVHLPLF RFDDVYLGIV ALKAGISLQH CDDFRFHRPA YKGPDSYSSV
310 320
IASHEFGDPE EMTRVWNECR SANYA
Length:325
Mass (Da):37,620
Last modified:May 10, 2004 - v2
Checksum:i0DF89B720F43657B
GO

Sequence cautioni

The sequence AAL48007 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142E → D in AAA85211 (PubMed:9012507).Curated1
Sequence conflicti163D → E in AAA85211 (PubMed:9012507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41449 mRNA. Translation: AAA85211.1.
AE014298 Genomic DNA. Translation: AAF45918.1.
AL033125 Genomic DNA. Translation: CAA21833.1.
BT021250 mRNA. Translation: AAX33398.1.
AY070536 mRNA. Translation: AAL48007.1. Different initiation.
RefSeqiNP_476901.1. NM_057553.5.
UniGeneiDm.1589.

Genome annotation databases

EnsemblMetazoaiFBtr0070638; FBpp0070606; FBgn0000221.
GeneIDi31358.
KEGGidme:Dmel_CG4934.

Similar proteinsi

Entry informationi

Entry nameiBRN_DROME
AccessioniPrimary (citable) accession number: Q24157
Secondary accession number(s): Q5BIH4, Q8SZS9, Q9W4N0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: August 30, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families