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Q24151 (STAT_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducer and transcription activator

Short name=d-STAT
Alternative name(s):
Protein marelle
Gene names
Name:Stat92E
Synonyms:MARE, mrL, Stat
ORF Names:CG4257
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length761 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression. The Janus kinase-STAT pathway is connected to drosophila early development.

Subunit structure

Forms a homodimer or a heterodimer with a related family member.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Translocated into the nucleus in response to phosphorylation By similarity.

Developmental stage

Expressed throughout embryonic, larval, pupal, and adult stages, with some decrease in the late embryonic stages. The expression is uniform in unfertilized or just fertilized eggs, suggesting maternally deposited mRNA. At blastoderm stage, expression pattern shows stripes, that are reminiscent of many pair rule genes pattern.

Post-translational modification

Tyrosine phosphorylated by hopscotch. Phosphorylation is required for DNA-binding activity and dimerization. Ref.2

Disruption phenotype

Flies exhibit aberrant expression of the pair rule gene even-skipped at the cellular blastoderm stage, leading to larval segmentation defects. Ref.1 Ref.2

Sequence similarities

Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.

Sequence caution

The sequence AAM11192.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainSH2 domain
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJAK-STAT cascade

Traceable author statement PubMed 12194841. Source: FlyBase

blastoderm segmentation

Inferred from mutant phenotype PubMed 11746233. Source: FlyBase

border follicle cell migration

Inferred from mutant phenotype PubMed 11779460PubMed 19350016. Source: FlyBase

cell dedifferentiation

Inferred from expression pattern PubMed 19664993. Source: FlyBase

cellular response to X-ray

Inferred from mutant phenotype PubMed 18779571. Source: FlyBase

cellular response to lipopolysaccharide

Inferred from direct assay PubMed 10764759. Source: FlyBase

compound eye development

Traceable author statement PubMed 11746233. Source: FlyBase

defense response

Inferred from direct assay PubMed 10764759. Source: FlyBase

epithelial cell fate determination, open tracheal system

Traceable author statement PubMed 14570584. Source: FlyBase

eye-antennal disc morphogenesis

Inferred from mutant phenotype PubMed 17079268. Source: FlyBase

germ-line stem cell division

Traceable author statement PubMed 12459723. Source: FlyBase

germ-line stem cell maintenance

Inferred from mutant phenotype PubMed 15143218. Source: FlyBase

hemocyte proliferation

Traceable author statement PubMed 14602069. Source: FlyBase

hemopoiesis

Non-traceable author statement PubMed 11746233. Source: FlyBase

hindgut morphogenesis

Inferred from mutant phenotype PubMed 12441298PubMed 14654218. Source: FlyBase

humoral immune response

Traceable author statement PubMed 15199955. Source: FlyBase

imaginal disc-derived wing morphogenesis

Non-traceable author statement PubMed 11746233. Source: FlyBase

lamellocyte differentiation

Non-traceable author statement PubMed 15199955. Source: FlyBase

larval lymph gland hemopoiesis

Inferred from mutant phenotype PubMed 19737914. Source: FlyBase

larval somatic muscle development

Inferred from mutant phenotype PubMed 19217429. Source: FlyBase

long-term memory

Inferred from mutant phenotype PubMed 21518857. Source: FlyBase

negative regulation of Wnt signaling pathway

Inferred from mutant phenotype PubMed 17079268. Source: FlyBase

negative regulation of antimicrobial humoral response

Inferred from mutant phenotype PubMed 17803358. Source: FlyBase

nervous system development

Inferred from mutant phenotype PubMed 14654218. Source: FlyBase

oocyte localization involved in germarium-derived egg chamber formation

Inferred from mutant phenotype PubMed 14536057. Source: FlyBase

oogenesis

Inferred from mutant phenotype PubMed 11846485PubMed 14536057. Source: FlyBase

open tracheal system development

Inferred from mutant phenotype PubMed 14654218. Source: FlyBase

ovarian follicle cell stalk formation

Inferred from mutant phenotype PubMed 14536057. Source: FlyBase

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 21176204. Source: FlyBase

positive regulation of defense response to virus by host

Inferred from mutant phenotype PubMed 19763182. Source: FlyBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10764759. Source: GOC

primary sex determination

Inferred from mutant phenotype PubMed 10882142. Source: FlyBase

regulation of JAK-STAT cascade

Inferred from mutant phenotype PubMed 20624926. Source: FlyBase

segment specification

Inferred from mutant phenotype PubMed 16277982. Source: FlyBase

sex determination

Non-traceable author statement PubMed 11746233. Source: FlyBase

somatic stem cell division

Traceable author statement PubMed 12459723. Source: FlyBase

somatic stem cell maintenance

Inferred from mutant phenotype PubMed 20143337. Source: FlyBase

stem cell division

Inferred from mutant phenotype PubMed 11752575. Source: FlyBase

stem cell maintenance

Inferred from mutant phenotype PubMed 11752574. Source: FlyBase

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12967563. Source: FlyBase

nucleus

Inferred from direct assay PubMed 19948068. Source: BHF-UCL

   Molecular_functionDNA binding

Traceable author statement PubMed 11746233. Source: FlyBase

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 10764759. Source: FlyBase

calcium ion binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 12586062. Source: FlyBase

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 12194841. Source: FlyBase

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform F (identifier: Q24151-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q24151-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.
     699-705: Missing.
Note: No experimental confirmation available.
Isoform C (identifier: Q24151-2)

Also known as: E; Short;

The sequence of this isoform differs from the canonical sequence as follows:
     699-705: Missing.
Isoform G (identifier: Q24151-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 761761Signal transducer and transcription activator
PRO_0000182435

Regions

Domain594 – 65865SH2

Amino acid modifications

Modified residue7111Phosphotyrosine; by JAK Ref.2 Ref.7

Natural variations

Alternative sequence1 – 126126Missing in isoform B and isoform G.
VSP_012752
Alternative sequence699 – 7057Missing in isoform B and isoform C.
VSP_006290

Experimental info

Sequence conflict1051S → T in AAB02195. Ref.2
Sequence conflict6481L → H in AAB02195. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform F (Long) [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: BF3A622A29899161

FASTA76186,415
        10         20         30         40         50         60 
MSLWKRISSH VDCEQRMAAY YEEKGMLELR LCLAPWIEDR IMSEQITPNT TDQLERVALK 

        70         80         90        100        110        120 
FNEDLQQKLL STRTASDQAL KFRVVELCAL IQRISAVELY THLRSGLQKE LQLVTEKSVA 

       130        140        150        160        170        180 
ATAGQSMPLN PYNMNNTPMV TGYMVDPSDL LAVSNSCNPP VVQGIGPIHN VQNTGIASPA 

       190        200        210        220        230        240 
LGMVTPKVEL YEVQHQIMQS LNEFGNCANA LKLLAQNYSY MLNSTSSPNA EAAYRSLIDE 

       250        260        270        280        290        300 
KAAIVLTMRR SFMYYESLHE MVIHELKNWR HQQAQAGNGA PFNEGSLDDI QRCFEMLESF 

       310        320        330        340        350        360 
IAHMLAAVKE LMRVRLVTEE PELTHLLEQV QNAQKNLVCS AFIVDKQPPQ VMKTNTRFAA 

       370        380        390        400        410        420 
SVRWLIGSQL GIHNNPPTVE CIIMSEIQSQ RFVTRNTQMD NSSLSGQSSG EIQNASSTME 

       430        440        450        460        470        480 
YQQNNHVFSA SFRNMQLKKI KRAEKKGTES VMDEKFALFF YTTTTVNDFQ IRVWTLSLPV 

       490        500        510        520        530        540 
VVIVHGNQEP QSWATITWDN AFAEIVRDPF MITDRVTWAQ LSVALNIKFG SCTGRSLTID 

       550        560        570        580        590        600 
NLDFLYEKLQ REERSEYITW NQFCKEPMPD RSFTFWEWFF AIMKLTKDHM LGMWKAGCIM 

       610        620        630        640        650        660 
GFINKTKAQT DLLRSVYGIG TFLLRFSDSE LGGVTIAYVN ENGLVTMLAP WTARDFQVLN 

       670        680        690        700        710        720 
LADRIRDLDV LCWLHPSDRN ASPVKRDVAF GEFYSKRQEP EPLVLDPVTG YVKSTLHVHV 

       730        740        750        760 
CRNGENGSTS GTPHHAQESM QLGNGDFGMA DFDTITNFEN F 

« Hide

Isoform B [UniParc].

Checksum: 841208C04B2A91D0
Show »

FASTA62871,198
Isoform C (E) (Short) [UniParc].

Checksum: B607B3328A4A4956
Show »

FASTA75485,637
Isoform G [UniParc].

Checksum: A3B0A8FFC32BCCF3
Show »

FASTA63571,976

References

« Hide 'large scale' references
[1]"Marelle acts downstream of the Drosophila HOP/JAK kinase and encodes a protein similar to the mammalian STATs."
Hou X.S., Melnick M.B., Perrimon N.
Cell 84:411-419(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM F), DISRUPTION PHENOTYPE.
Tissue: Embryo.
[2]"Identification of a Stat gene that functions in Drosophila development."
Yan R., Small S., Desplan C., Dearolf C.R., Darnell J.E. Jr.
Cell 84:421-430(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), PHOSPHORYLATION AT TYR-711, DISRUPTION PHENOTYPE.
Tissue: Embryo.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[5]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM F).
Strain: Berkeley.
Tissue: Embryo.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 267-761 (ISOFORM F).
Strain: Berkeley.
Tissue: Larva and Pupae.
[7]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-711, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U40070 mRNA. Translation: AAC46984.1.
U46688 mRNA. Translation: AAB02195.1.
AE014297 Genomic DNA. Translation: AAN14350.2.
AE014297 Genomic DNA. Translation: AAN14351.2.
AE014297 Genomic DNA. Translation: AAS65180.1.
AE014297 Genomic DNA. Translation: AAX52969.1.
BT021314 mRNA. Translation: AAX33462.1.
AY094839 mRNA. Translation: AAM11192.1. Different initiation.
RefSeqNP_001014643.1. NM_001014643.2. [Q24151-4]
NP_001262757.1. NM_001275828.1. [Q24151-4]
NP_001262762.1. NM_001275833.1. [Q24151-1]
NP_732516.2. NM_169899.3. [Q24151-2]
NP_732517.2. NM_169900.3. [Q24151-3]
NP_996242.1. NM_206520.2. [Q24151-2]
NP_996243.1. NM_206521.2. [Q24151-1]
UniGeneDm.2627.

3D structure databases

ProteinModelPortalQ24151.
SMRQ24151. Positions 235-695.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid67407. 18 interactions.
DIPDIP-29510N.

Proteomic databases

PaxDbQ24151.
PRIDEQ24151.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0089486; FBpp0088489; FBgn0016917. [Q24151-1]
GeneID42428.
KEGGdme:Dmel_CG4257.

Organism-specific databases

CTD42428.
FlyBaseFBgn0016917. Stat92E.

Phylogenomic databases

eggNOGNOG245085.
GeneTreeENSGT00740000115175.
InParanoidQ24151.
KOK11224.
OMAALNIKFG.
OrthoDBEOG73JKTT.
PhylomeDBQ24151.

Enzyme and pathway databases

SignaLinkQ24151.

Gene expression databases

BgeeQ24151.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERPTHR11801. PTHR11801. 1 hit.
PfamPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMSSF47655. SSF47655. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi42428.
NextBio828722.

Entry information

Entry nameSTAT_DROME
AccessionPrimary (citable) accession number: Q24151
Secondary accession number(s): Q0KI43 expand/collapse secondary AC list , Q24181, Q59DV7, Q5BIB0, Q8IN54, Q8SX55, Q9VDL8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase