Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase shark

Gene

shark

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Following axon injury, required for recruitment of drpr and glial cells to severed axons and for glial clearance of severed axons from the central nervous system (PubMed:18432193). Together with Src42a and drpr, promotes the migration of macrophages to sites of wounding as part of a signaling cascade where Scr42a detects production of hydrogen peroxide at wound sites which triggers phosphorylation of drpr and subsequent recruitment and activation of shark (PubMed:26028435). May be involved in signal transduction on the apical surface of ectodermal epithelial cells, regulating their polarity during invagination (PubMed:7892198). Crumbs (crb) may be the intracellular signal (PubMed:7892198).3 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei698ATPPROSITE-ProRule annotation1
Active sitei789Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi668 – 676ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • apoptotic cell clearance Source: FlyBase
  • cell migration Source: GO_Central
  • dorsal appendage formation Source: FlyBase
  • dorsal closure Source: FlyBase
  • dorsal closure, elongation of leading edge cells Source: FlyBase
  • eggshell chorion assembly Source: FlyBase
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • maintenance of epithelial cell apical/basal polarity Source: UniProtKB
  • neuron projection morphogenesis Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • protein phosphorylation Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of glucose metabolic process Source: FlyBase
  • sleep Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-354192. Integrin alphaIIb beta3 signaling.
SignaLinkiQ24145.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase shark (EC:2.7.10.2)
Gene namesi
Name:shark
Synonyms:Tk7
ORF Names:CG18247
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015295. shark.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Apical expression in cephalic furrow and tracheal cells. Limited to luminal surface and absent from the basal surface.

GO - Cellular componenti

  • cell cortex Source: FlyBase
  • cytoplasm Source: UniProtKB
  • extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Strong reduction in the number of macrophages recruited to wound sites (PubMed:26028435). RNAi-mediated knockdown in glial cells abolishes a number of events which normally occur following axon injury including drpr localization at severed axons, glial recruitment to severed axons, up-regulation of drpr in glial cells and clearance of axonal debris from the central nervous system (PubMed:18432193).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi698K → R: Kinase-dead. No effect on interaction with drpr. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881371 – 939Tyrosine-protein kinase sharkAdd BLAST939

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei927PhosphotyrosineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24145.
PRIDEiQ24145.

Expressioni

Tissue specificityi

Gastrulation embryos show expression in ectodermal cells along the cephalic furrow and ventral midline. Proctodeum, stomodeum and their derived structures (foregut, atrium, pharynx, esophagus and hindgut) continue to show expression from stage 8-9 to late embryos. Other ectodermally derived structures (frontal sac, salivary gland and labium) and developing tracheal system also show expression.1 Publication

Developmental stagei

Embryos only.1 Publication

Gene expression databases

BgeeiFBgn0015295.
GenevisibleiQ24145. DM.

Interactioni

Subunit structurei

Interacts with drpr; this is required for the recruitment of drpr and glial cells to severed axons and for the phagocytosis of axonal debris by glial cells following axon injury.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68999. 1 interactor.
DIPiDIP-59843N.
IntActiQ24145. 1 interactor.
STRINGi7227.FBpp0086382.

Structurei

3D structure databases

ProteinModelPortaliQ24145.
SMRiQ24145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 106SH2 1PROSITE-ProRule annotationCuratedAdd BLAST97
Repeati153 – 185ANK 1CuratedAdd BLAST33
Repeati186 – 218ANK 2CuratedAdd BLAST33
Repeati220 – 252ANK 3CuratedAdd BLAST33
Domaini288 – 403SH2 2PROSITE-ProRule annotationCuratedAdd BLAST116
Domaini662 – 921Protein kinasePROSITE-ProRule annotationAdd BLAST260

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, SH2 domain

Phylogenomic databases

eggNOGiENOG410IH0T. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118799.
InParanoidiQ24145.
KOiK17512.
OMAiLCPAYIY.
OrthoDBiEOG091G04J0.
PhylomeDBiQ24145.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00248. ANK. 4 hits.
SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRDSDPMKW YHGNLSREAA DELLKQGYED GTFLVRESST AAGDFVLSLL
60 70 80 90 100
CQGEVCHYQV RRHGGEDAFF SIDDKVQTKI LHGLDTLVDY YQQAANGLPT
110 120 130 140 150
KLTVPLIRDL PPHNTRSHGV TNLLHRATSK NESKVVFELL KCGYRNFDAK
160 170 180 190 200
NQDGQTALHL AALHSDEDIL KHLLNAKVQV NSSDSFGCQP LHYAARSKPA
210 220 230 240 250
SFIRTLISAQ ANVQGRNIDN GYVPLHEAAK HGNLEAVQEL LLAEAPPLPR
260 270 280 290 300
TSSGEFPFDL AKEAGQTAVE EFLLNYKLPP ANTTRDQWYH GTLTREEAVA
310 320 330 340 350
ILKKHAKELL AKQPEVDTSG CFLVRYSESP AASGLVLTLL CDQVVKNFRI
360 370 380 390 400
SQADLYQNGN KVQSGGSKFL YIDDGPYWPS VEHLIAHFMR FSYGLPVSLK
410 420 430 440 450
YPVPPQPKPE VPSFATIPRS NMKPKAASPA TPPTPVSPHS HHQHPHVPAL
460 470 480 490 500
TITKKKQKEN SSSMFNTLRL TSPKKALFDM NSLRKNKSKG KRSDSESSVS
510 520 530 540 550
GSLAGTEQEL QAAAPMLKSL SFSTEFSTFN ADGVTGSGAA AAGEVYNVPR
560 570 580 590 600
NNTPIEIDLP PIAQKTEAEV EYFTKSDVAI ERERAGQWIG NGYQPTMDVL
610 620 630 640 650
SLLDQQIKAP AVARLNSLGP NASTESEMAS YLHRKCSGTP STPSATEVEA
660 670 680 690 700
AKLRFFIEPE KLVLDREIGH GEFGSVHSGW LLRKSGAGEE SRLEVAIKML
710 720 730 740 750
SDEHSNKQEF LREASVMMRL EHKCIVRLIG IAKGEMLMMV QELAPLGSML
760 770 780 790 800
QYILDHGHEI TANAELKVWA SQIACGMHYL ESQHFVHRDL AARNILLTAR
810 820 830 840 850
HQAKISDFGM SRSLRPGSTE YQFTQGGRWP IRWYAPESFN LGIFSHASDV
860 870 880 890 900
WSFGVTIWEM FSLGAPPYGE ISNVDAIKLV DSGERLPQPN LCPAYIYAVM
910 920 930
QSCWKERPKD RPTFVYLTEF FARDPDYQNL PELVQTVHI
Length:939
Mass (Da):104,273
Last modified:October 1, 2000 - v2
Checksum:i37CC2C3DA25D3F52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19A → V in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti39S → R in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti50L → F in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti85D → E in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti129S → T in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti199P → T in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti341C → S in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti505 – 506GT → RA in AAA79851 (PubMed:7892198).Curated2
Sequence conflicti597M → V in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti611A → P in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti732A → S in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti898A → P in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti935 – 939QTVHI → KRFTFNPVSIFHFFRC in AAA79851 (PubMed:7892198).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37773 mRNA. Translation: AAA79851.1.
AE013599 Genomic DNA. Translation: AAF58044.1.
AY051937 mRNA. Translation: AAK93361.1.
S55982 Genomic DNA. Translation: AAB19909.1.
PIRiS18015.
RefSeqiNP_524743.2. NM_080004.4.
UniGeneiDm.6764.

Genome annotation databases

EnsemblMetazoaiFBtr0087244; FBpp0086382; FBgn0015295.
GeneIDi44353.
KEGGidme:Dmel_CG18247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37773 mRNA. Translation: AAA79851.1.
AE013599 Genomic DNA. Translation: AAF58044.1.
AY051937 mRNA. Translation: AAK93361.1.
S55982 Genomic DNA. Translation: AAB19909.1.
PIRiS18015.
RefSeqiNP_524743.2. NM_080004.4.
UniGeneiDm.6764.

3D structure databases

ProteinModelPortaliQ24145.
SMRiQ24145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68999. 1 interactor.
DIPiDIP-59843N.
IntActiQ24145. 1 interactor.
STRINGi7227.FBpp0086382.

Proteomic databases

PaxDbiQ24145.
PRIDEiQ24145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087244; FBpp0086382; FBgn0015295.
GeneIDi44353.
KEGGidme:Dmel_CG18247.

Organism-specific databases

CTDi44353.
FlyBaseiFBgn0015295. shark.

Phylogenomic databases

eggNOGiENOG410IH0T. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118799.
InParanoidiQ24145.
KOiK17512.
OMAiLCPAYIY.
OrthoDBiEOG091G04J0.
PhylomeDBiQ24145.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-354192. Integrin alphaIIb beta3 signaling.
SignaLinkiQ24145.

Miscellaneous databases

ChiTaRSishark. fly.
GenomeRNAii44353.
PROiQ24145.

Gene expression databases

BgeeiFBgn0015295.
GenevisibleiQ24145. DM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00248. ANK. 4 hits.
SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHARK_DROME
AccessioniPrimary (citable) accession number: Q24145
Secondary accession number(s): Q26299, Q9V7K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.