SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q24145

- SHARK_DROME

UniProt

Q24145 - SHARK_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Tyrosine-protein kinase shark
Gene
shark, Tk7, CG18247
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

May be involved in signal transduction on the apical surface of ectodermal epithelia regulating their polarity during invagination. Crumbs (crb) may be the intracellular signal.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei698 – 6981ATP By similarity
Active sitei789 – 7891Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi668 – 6769ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: FlyBase
  3. protein tyrosine kinase activity Source: FlyBase

GO - Biological processi

  1. JNK cascade Source: FlyBase
  2. apoptotic cell clearance Source: FlyBase
  3. dorsal appendage formation Source: FlyBase
  4. dorsal closure Source: FlyBase
  5. dorsal closure, elongation of leading edge cells Source: FlyBase
  6. eggshell chorion assembly Source: FlyBase
  7. maintenance of epithelial cell apical/basal polarity Source: UniProtKB
  8. neuron projection morphogenesis Source: FlyBase
  9. peptidyl-tyrosine phosphorylation Source: GOC
  10. protein phosphorylation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
SignaLinkiQ24145.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase shark (EC:2.7.10.2)
Gene namesi
Name:shark
Synonyms:Tk7
ORF Names:CG18247
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015295. shark.

Subcellular locationi

Cytoplasm
Note: Apical expression in cephalic furrow and tracheal cells. Limited to luminal surface and absent from the basal surface.1 Publication

GO - Cellular componenti

  1. cell cortex Source: FlyBase
  2. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Tyrosine-protein kinase shark
PRO_0000088137Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei927 – 9271Phosphotyrosine Reviewed prediction

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24145.

Expressioni

Tissue specificityi

Gastrulation embryos show expression in ectodermal cells along the cephalic furrow and ventral midline. Proctodeum, stomodeum and their derived structures (foregut, atrium, pharynx, esophagus and hindgut) continue to show expression from stage 8-9 to late embryos. Other ectodermally derived structures (frontal sac, salivary gland and labium) and developing tracheal system also show expression.1 Publication

Developmental stagei

Embryos only.1 Publication

Gene expression databases

BgeeiQ24145.

Interactioni

Protein-protein interaction databases

BioGridi68999. 1 interaction.
DIPiDIP-59843N.

Structurei

3D structure databases

ProteinModelPortaliQ24145.
SMRiQ24145. Positions 10-274, 285-404, 614-928.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 10697SH2 1
Add
BLAST
Repeati153 – 18533ANK 1
Add
BLAST
Repeati186 – 21833ANK 2
Add
BLAST
Repeati220 – 25233ANK 3
Add
BLAST
Domaini288 – 403116SH2 2
Add
BLAST
Domaini662 – 921260Protein kinase
Add
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.
Contains 2 SH2 domains.

Keywords - Domaini

ANK repeat, Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115195.
InParanoidiQ24145.
KOiK17512.
OMAiHGEDAFF.
OrthoDBiEOG70W3CT.
PhylomeDBiQ24145.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00248. ANK. 4 hits.
SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q24145-1 [UniParc]FASTAAdd to Basket

« Hide

MSRDSDPMKW YHGNLSREAA DELLKQGYED GTFLVRESST AAGDFVLSLL    50
CQGEVCHYQV RRHGGEDAFF SIDDKVQTKI LHGLDTLVDY YQQAANGLPT 100
KLTVPLIRDL PPHNTRSHGV TNLLHRATSK NESKVVFELL KCGYRNFDAK 150
NQDGQTALHL AALHSDEDIL KHLLNAKVQV NSSDSFGCQP LHYAARSKPA 200
SFIRTLISAQ ANVQGRNIDN GYVPLHEAAK HGNLEAVQEL LLAEAPPLPR 250
TSSGEFPFDL AKEAGQTAVE EFLLNYKLPP ANTTRDQWYH GTLTREEAVA 300
ILKKHAKELL AKQPEVDTSG CFLVRYSESP AASGLVLTLL CDQVVKNFRI 350
SQADLYQNGN KVQSGGSKFL YIDDGPYWPS VEHLIAHFMR FSYGLPVSLK 400
YPVPPQPKPE VPSFATIPRS NMKPKAASPA TPPTPVSPHS HHQHPHVPAL 450
TITKKKQKEN SSSMFNTLRL TSPKKALFDM NSLRKNKSKG KRSDSESSVS 500
GSLAGTEQEL QAAAPMLKSL SFSTEFSTFN ADGVTGSGAA AAGEVYNVPR 550
NNTPIEIDLP PIAQKTEAEV EYFTKSDVAI ERERAGQWIG NGYQPTMDVL 600
SLLDQQIKAP AVARLNSLGP NASTESEMAS YLHRKCSGTP STPSATEVEA 650
AKLRFFIEPE KLVLDREIGH GEFGSVHSGW LLRKSGAGEE SRLEVAIKML 700
SDEHSNKQEF LREASVMMRL EHKCIVRLIG IAKGEMLMMV QELAPLGSML 750
QYILDHGHEI TANAELKVWA SQIACGMHYL ESQHFVHRDL AARNILLTAR 800
HQAKISDFGM SRSLRPGSTE YQFTQGGRWP IRWYAPESFN LGIFSHASDV 850
WSFGVTIWEM FSLGAPPYGE ISNVDAIKLV DSGERLPQPN LCPAYIYAVM 900
QSCWKERPKD RPTFVYLTEF FARDPDYQNL PELVQTVHI 939
Length:939
Mass (Da):104,273
Last modified:October 1, 2000 - v2
Checksum:i37CC2C3DA25D3F52
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → V in AAA79851. 1 Publication
Sequence conflicti39 – 391S → R in AAA79851. 1 Publication
Sequence conflicti50 – 501L → F in AAA79851. 1 Publication
Sequence conflicti85 – 851D → E in AAA79851. 1 Publication
Sequence conflicti129 – 1291S → T in AAA79851. 1 Publication
Sequence conflicti199 – 1991P → T in AAA79851. 1 Publication
Sequence conflicti341 – 3411C → S in AAA79851. 1 Publication
Sequence conflicti505 – 5062GT → RA in AAA79851. 1 Publication
Sequence conflicti597 – 5971M → V in AAA79851. 1 Publication
Sequence conflicti611 – 6111A → P in AAA79851. 1 Publication
Sequence conflicti732 – 7321A → S in AAA79851. 1 Publication
Sequence conflicti898 – 8981A → P in AAA79851. 1 Publication
Sequence conflicti935 – 9395QTVHI → KRFTFNPVSIFHFFRC in AAA79851. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37773 mRNA. Translation: AAA79851.1.
AE013599 Genomic DNA. Translation: AAF58044.1.
AY051937 mRNA. Translation: AAK93361.1.
S55982 Genomic DNA. Translation: AAB19909.1.
PIRiS18015.
RefSeqiNP_524743.2. NM_080004.3.
UniGeneiDm.6764.

Genome annotation databases

EnsemblMetazoaiFBtr0087244; FBpp0086382; FBgn0015295.
GeneIDi44353.
KEGGidme:Dmel_CG18247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37773 mRNA. Translation: AAA79851.1 .
AE013599 Genomic DNA. Translation: AAF58044.1 .
AY051937 mRNA. Translation: AAK93361.1 .
S55982 Genomic DNA. Translation: AAB19909.1 .
PIRi S18015.
RefSeqi NP_524743.2. NM_080004.3.
UniGenei Dm.6764.

3D structure databases

ProteinModelPortali Q24145.
SMRi Q24145. Positions 10-274, 285-404, 614-928.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 68999. 1 interaction.
DIPi DIP-59843N.

Proteomic databases

PaxDbi Q24145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0087244 ; FBpp0086382 ; FBgn0015295 .
GeneIDi 44353.
KEGGi dme:Dmel_CG18247.

Organism-specific databases

CTDi 44353.
FlyBasei FBgn0015295. shark.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115195.
InParanoidi Q24145.
KOi K17512.
OMAi HGEDAFF.
OrthoDBi EOG70W3CT.
PhylomeDBi Q24145.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 1994.
SignaLinki Q24145.

Miscellaneous databases

ChiTaRSi shark. drosophila.
GenomeRNAii 44353.
NextBioi 837182.

Gene expression databases

Bgeei Q24145.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.30.505.10. 2 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF00023. Ank. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00248. ANK. 4 hits.
SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Shark, a Src homology 2, ankyrin repeat, tyrosine kinase, is expressed on the apical surfaces of ectodermal epithelia."
    Ferrante A.W. Jr., Reinke R., Stanley E.R.
    Proc. Natl. Acad. Sci. U.S.A. 92:1911-1915(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Identification of seven novel protein-tyrosine kinase genes of Drosophila by the polymerase chain reaction."
    Shishido E., Emori Y., Saigo K.
    FEBS Lett. 289:235-238(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 793-849.

Entry informationi

Entry nameiSHARK_DROME
AccessioniPrimary (citable) accession number: Q24145
Secondary accession number(s): Q26299, Q9V7K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi