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Q24139 (COMM_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein commissureless
Gene names
Name:comm
ORF Names:CG17943
OrganismDrosophila melanogaster (Fruit fly)
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls axon guidance across the CNS midline by preventing the delivery of Robo to the growth cone. Ref.4 Ref.5

Subunit structure

Interacts with Nedd4 and Robo. Ref.4

Subcellular location

Cytoplasmic vesicle membrane; Single-pass membrane protein Potential. Cell membrane; Single-pass membrane protein Potential Ref.4 Ref.5.

Domain

The cytoplasmic domain is required for function in axon guidance.

Post-translational modification

According to Ref.4, ubiquitinated by Nedd4; which promotes endocytosis of the comm/robo complex and comm proteasomal degradation. According to Ref.5, not ubiquitinated by Nedd4.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Protein commissureless
PRO_0000090013

Regions

Topological domain1 – 136136Extracellular Potential
Transmembrane137 – 15721Helical; Potential
Topological domain158 – 370213Cytoplasmic Potential
Region108 – 13124Required for vesicular localization
Region220 – 2234Interaction with Nedd4
Region229 – 2324Interaction with Nedd4
Compositional bias12 – 154Poly-Thr
Compositional bias258 – 2625Poly-His
Compositional bias283 – 2864Poly-Ala
Compositional bias294 – 3018Poly-Ser
Compositional bias365 – 3684Poly-Val

Experimental info

Mutagenesis2211P → A: Abolishes interaction with Nedd4, ubiquitination and internalization; when associated with A-230. Ref.4
Mutagenesis2231Y → A: No effect on interaction with Nedd4. Ref.4
Mutagenesis2301P → A: Abolishes interaction with Nedd4, ubiquitination and internalization; when associated with A-221. Ref.4
Mutagenesis2321Y → A: No effect on interaction with Nedd4. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q24139 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EB298EA415D4FBDA

FASTA37040,311
        10         20         30         40         50         60 
MISTTDYPTV ETTTTAEELY AEYISAPASS MSPAAIAEHL QQNQITFEIP SAHDLRHIDA 

        70         80         90        100        110        120 
LNSFNALLQR IGNAAVSYDP APPSGWSPDG SISTEQLSKS VVLDLADLRD RSEESGESSW 

       130        140        150        160        170        180 
WSQIFGDADM HVIINYLWIG VVSSLVILSL VFILFSCYFY RKFRTWKKCN KDIRAQIHAA 

       190        200        210        220        230        240 
SDSYSSHLVG CDASRLLLHQ QMQHPHHRSS EAGFYQIESP PCYTIATGLP SYDEALHHQP 

       250        260        270        280        290        300 
RHFAYGMKFV YPSLAAVHHH HHCISNWEKQ EPLNKLQKCK LSAAAAVEED KADSSSSTSA 

       310        320        330        340        350        360 
SASPSSSESS NLATATPAIC INMPSGRQDE EVDNSDSDSA IAVAVAVAQS LQPAAPADDD 

       370 
CASLVVVVAA

« Hide

References

« Hide 'large scale' references
[1]"Commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein."
Tear G., Harris R., Sutaria S., Kilomanski K., Goodman C.S., Seeger M.A.
Neuron 16:501-514(1996) [PubMed: 8785048] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]"Drosophila Nedd4, a ubiquitin ligase, is recruited by Commissureless to control cell surface levels of the roundabout receptor."
Myat A., Henry P., McCabe V., Flintoft L., Rotin D., Tear G.
Neuron 35:447-459(2002) [PubMed: 12165468] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NEDD4 AND ROBO, UBIQUITINATION, MUTAGENESIS OF PRO-221; TYR-223; PRO-230 AND TYR-232, SUBCELLULAR LOCATION.
[5]"Comm function in commissural axon guidance: cell-autonomous sorting of Robo in vivo."
Keleman K., Ribeiro C., Dickson B.J.
Nat. Neurosci. 8:156-163(2005) [PubMed: 15657595] [Abstract]
Cited for: FUNCTION, LACK OF UBIQUITINATION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U35854 mRNA. Translation: AAC47019.1.
AE014296 Genomic DNA. Translation: AAF49584.1.
RefSeqNP_524095.1. NM_079371.3.
UniGeneDm.2546.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EZ5NMR-P227-237[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-22799N.
IntActQ24139. 1 interaction.
MINTMINT-345339.
STRINGQ24139.

Proteomic databases

PRIDEQ24139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0075579; FBpp0075332; FBgn0010105.
GeneID39717.
KEGGdme:Dmel_CG17943.
NMPDRfig|7227.3.peg.9993.
UCSCCG17943-RA. d. melanogaster.

Organism-specific databases

CTD39717.
FlyBaseFBgn0010105. comm.

Phylogenomic databases

GeneTreeEMGT00050000011363.
InParanoidQ24139.
OMANYLWIGV.
OrthoDBEOG437PWW.
PhylomeDBQ24139.

Gene expression databases

ArrayExpressQ24139.
BgeeQ24139.
GermOnlineCG17943. Drosophila melanogaster.

Family and domain databases

ProtoNetSearch...

Other

NextBio815023.

Entry information

Entry nameCOMM_DROME
AccessionPrimary (citable) accession number: Q24139
Secondary accession number(s): Q9VUT9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

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