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Protein

Protein trachealess

Gene

trh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor, master regulator of tracheal cell fates in the embryo, necessary for the development of the salivary gland duct and the posterior spiracles. It may induce a general fate of branched tubular structures of epithelial origin. Heterodimers of tgo/trh are involved in the control of breathless expression.2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • dendrite morphogenesis Source: FlyBase
  • epithelial cell fate determination, open tracheal system Source: FlyBase
  • epithelial tube formation Source: FlyBase
  • lateral inhibition Source: FlyBase
  • limb development Source: FlyBase
  • Malpighian tubule morphogenesis Source: FlyBase
  • open tracheal system development Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • salivary gland development Source: UniProtKB
  • salivary gland morphogenesis Source: FlyBase
  • spiracle morphogenesis, open tracheal system Source: FlyBase
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein trachealess
Gene namesi
Name:trh
ORF Names:CG6883
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0262139. trh.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Tube-forming cells of the salivary gland, trachea, and filzkorper fail to invaginate to form tubes and remain on the embryo surface.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi579 – 5791S → A: No effect on phosphorylation by Akt1; no effect on transcriptional activity. 1 Publication
Mutagenesisi673 – 6731S → A: Abolishes phosphorylation by Akt1, nuclear localization, and transcriptional activity. 1 Publication
Mutagenesisi673 – 6731S → D: Slightly increases transcriptional activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10221022Protein trachealessPRO_0000127476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei673 – 6731Phosphoserine; by PKB/Akt11 Publication

Post-translational modificationi

Ser-673 phosphorylation by PKB/Akt1 is required for nuclear targeting and transcriptional activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24119.

PTM databases

iPTMnetiQ24119.

Expressioni

Tissue specificityi

Trachea, salivary gland ducts, posterior spiracles (Filzkoeper primordia) and a subset of cells in the CNS.2 Publications

Developmental stagei

During embryogenesis, first detected in the tracheal placodes at stage 8, and expression continues throughout embryonic and larval development. In the developing salivary gland, expression is observed in the entire gland at stage 9 and by stage 12, expression is confined to the salivary ducts.1 Publication

Gene expression databases

BgeeiFBgn0262139.
ExpressionAtlasiQ24119. differential.
GenevisibleiQ24119. DM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer with tgo.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi63620. 3 interactions.
STRINGi7227.FBpp0303179.

Structurei

3D structure databases

ProteinModelPortaliQ24119.
SMRiQ24119. Positions 84-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 13954bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini174 – 24471PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 46171PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini465 – 50844PACAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi629 – 6368Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi244 – 28138Gly-richAdd
BLAST
Compositional biasi976 – 9849His-rich

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3558. Eukaryota.
ENOG410YK57. LUCA.
GeneTreeiENSGT00760000118788.
InParanoidiQ24119.
KOiK09098.
OrthoDBiEOG091G02J4.

Family and domain databases

InterProiIPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: Q24119-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPYQAAVAM DYAGYQRQPT PGHPGSHMAT MGSLGMPAVP FTHSWMVPTQ
60 70 80 90 100
DLCAMPPYNK MTGHQQPPGA GMHAQQQPLE PGILELRKEK SRDAARSRRG
110 120 130 140 150
KENYEFYELA KMLPLPAAIT SQLDKASIIR LTISYLKLRD FSGHGDPPWT
160 170 180 190 200
REASSSSKLK SAAIRRSPAV DLFEQHQGTH ILQSLDGFAL AVAADGRFLY
210 220 230 240 250
ISETVSIYLG LSQVEMTGSS IFDYIHQADH SEIADQLGLS LTSGGGGGGG
260 270 280 290 300
SSSSGGGGGG AGGGMASPTS GASDDGSGTH GTNNPDVAAS MTQASTSGYK
310 320 330 340 350
GYDRSFCVRM KSTLTKRGCH FKSSGYRASD ATSNCNNGNN ASNNAKNVKN
360 370 380 390 400
PGSNYSVVLL LCKLRPQYTF SHSRKSQPPL LGMVALAIAL PPPSVHEIRL
410 420 430 440 450
ECDMFVTRVN FDLRVAHCEP RVSDLLDYSP EDLVNKSLYS LCHAEDANRL
460 470 480 490 500
RKSHSDLIEK GQVLTGYYRL MNKSGGYTWL QTCATVVCST KNADEQNIIC
510 520 530 540 550
VNYVISNREN ENMILDCCQL EPSPDSIKHE EGLGNDKSSG SPGGDASGEG
560 570 580 590 600
NSHLSAGDMK LNSPKTDSEG HSHRGRGRSA AASHGSSMNS LTMIKDSPTP
610 620 630 640 650
LGVEIDSGVL PTTVATPVPA ATPPVQSTKR KRKTKASQHA EDQGQEQVIS
660 670 680 690 700
EQPLPKLPTM EQRDQQPRSR LPSIVDEQPS SAADSAVKDL EQAMSKHLPS
710 720 730 740 750
PAAVVSVAPP NTDFSADSLL KQQQQQQQLD PNEKSSTIQW IGTPYQQPPA
760 770 780 790 800
PMPATALLRQ LYANRESVIR ATARQTPTGV GPGVFYGDQQ TGPLPTPPGS
810 820 830 840 850
ESSYENQYLQ LHSAASGGHP GGQKTSADAF TNLVSTYGGY HSSIDYHNAM
860 870 880 890 900
TPPSSVSPRD SNQPGKAAPV LASNGGYDYA PDPLRGQYAT SSGDVVPATL
910 920 930 940 950
PLKPQASYTA TMHPSGSTTT EGGVTYSNLD QPQYFAPHSS FHLYHKGSPA
960 970 980 990 1000
SGWYSTPSXV VDDQGQVPPS CQDQYHHHHH HHHHQDGSAG SSASQASERW
1010 1020
DFVGALGKVA RMFFSARKGN PG
Note: No experimental confirmation available.
Length:1,022
Mass (Da):109,260
Last modified:June 13, 2012 - v4
Checksum:iCDF4C8CE3FB38E37
GO
Isoform A (identifier: Q24119-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     959-1022: Missing.

Show »
Length:958
Mass (Da):102,242
Checksum:i8F9CF758F1370541
GO
Isoform B (identifier: Q24119-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-356: Missing.
     959-1022: Missing.

Show »
Length:929
Mass (Da):99,332
Checksum:iDA164A0B869F4BAF
GO
Isoform C (identifier: Q24119-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-82: LPYQAAVAMD...HAQQQPLEPG → EHHGSGFVAS...GAAQSSVNAR
     328-356: Missing.
     959-1022: Missing.

Note: No experimental confirmation available.
Show »
Length:966
Mass (Da):103,568
Checksum:i50144CA76E353D03
GO
Isoform E (identifier: Q24119-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-286: Missing.
     959-1022: Missing.

Show »
Length:952
Mass (Da):101,643
Checksum:i4B539CF8FBE67EA6
GO

Sequence cautioni

The sequence AAA96257 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA96754 differs from that shown. Reason: Frameshift at positions 22 and 35. Curated
The sequence AAM11264 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781P → A in AAA96257 (PubMed:8557198).Curated
Sequence conflicti250 – 2501G → GG in AAA96754 (PubMed:8557189).Curated
Sequence conflicti627 – 6271S → P in ABX00736 (Ref. 5) Curated
Sequence conflicti703 – 7031A → T in AAA96257 (PubMed:8557198).Curated
Sequence conflicti708 – 7081A → P in AAA96257 (PubMed:8557198).Curated
Sequence conflicti829 – 8291A → V in AAA96257 (PubMed:8557198).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 8281LPYQA…PLEPG → EHHGSGFVASPWAAVLGHHS MASDAGFAAAAAAAHVQNHS MHHPIHSHHHHHSHSHPHPH PHSHPHHHPHLGTAGGMPMD LHVPQGFPYYRYREDALCWG DRKSMEEIGAAQSSVNAR in isoform C. CuratedVSP_043931Add
BLAST
Alternative sequencei281 – 2866Missing in isoform E. 1 PublicationVSP_043932
Alternative sequencei328 – 35629Missing in isoform B and isoform C. 3 PublicationsVSP_043933Add
BLAST
Alternative sequencei959 – 102264Missing in isoform A, isoform B, isoform C and isoform E. 4 PublicationsVSP_043934Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33427 mRNA. Translation: AAA96257.1. Different initiation.
U42699 mRNA. Translation: AAA96754.1. Frameshift.
AE014296 Genomic DNA. Translation: AAF47386.1.
AE014296 Genomic DNA. Translation: ABI31226.1.
BT031114 mRNA. Translation: ABX00736.1.
AY094911 mRNA. Translation: AAM11264.1. Different initiation.
RefSeqiNP_001036575.1. NM_001043110.2. [Q24119-3]
NP_001097461.2. NM_001103991.2. [Q24119-6]
NP_001261207.1. NM_001274278.1. [Q24119-5]
NP_523872.2. NM_079148.3. [Q24119-1]
UniGeneiDm.7330.

Genome annotation databases

EnsemblMetazoaiFBtr0330146; FBpp0303179; FBgn0262139. [Q24119-5]
GeneIDi38065.
KEGGidme:Dmel_CG42865.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33427 mRNA. Translation: AAA96257.1. Different initiation.
U42699 mRNA. Translation: AAA96754.1. Frameshift.
AE014296 Genomic DNA. Translation: AAF47386.1.
AE014296 Genomic DNA. Translation: ABI31226.1.
BT031114 mRNA. Translation: ABX00736.1.
AY094911 mRNA. Translation: AAM11264.1. Different initiation.
RefSeqiNP_001036575.1. NM_001043110.2. [Q24119-3]
NP_001097461.2. NM_001103991.2. [Q24119-6]
NP_001261207.1. NM_001274278.1. [Q24119-5]
NP_523872.2. NM_079148.3. [Q24119-1]
UniGeneiDm.7330.

3D structure databases

ProteinModelPortaliQ24119.
SMRiQ24119. Positions 84-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63620. 3 interactions.
STRINGi7227.FBpp0303179.

PTM databases

iPTMnetiQ24119.

Proteomic databases

PaxDbiQ24119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0330146; FBpp0303179; FBgn0262139. [Q24119-5]
GeneIDi38065.
KEGGidme:Dmel_CG42865.

Organism-specific databases

CTDi7200.
FlyBaseiFBgn0262139. trh.

Phylogenomic databases

eggNOGiKOG3558. Eukaryota.
ENOG410YK57. LUCA.
GeneTreeiENSGT00760000118788.
InParanoidiQ24119.
KOiK09098.
OrthoDBiEOG091G02J4.

Miscellaneous databases

GenomeRNAii38065.
PROiQ24119.

Gene expression databases

BgeeiFBgn0262139.
ExpressionAtlasiQ24119. differential.
GenevisibleiQ24119. DM.

Family and domain databases

InterProiIPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRH_DROME
AccessioniPrimary (citable) accession number: Q24119
Secondary accession number(s): A8JNH0
, A8WHG8, Q0E8K2, Q24165, Q8SX13, Q9W0Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 13, 2012
Last modified: September 7, 2016
This is version 160 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Readthrough of the terminator UGA may occur between the codons for 958-Ser and 960-Val.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.